Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,200,913 T→A 5.8% K40N (AAA→AAT) ‡ xisE ← e14 prophage; putative excisionase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,200,9130TA 5.8% 399.1 / 10.3 139K40N (AAA→AAT) ‡xisEe14 prophage; putative excisionase
Reads supporting (aligned to +/- strand):  ref base T (57/74);  new base A (4/4);  total (61/78)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01

Only 100 of 142 total aligned reads displayed.

GTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGC  >  NC_000913/1200765‑1201062
                                                                                                                                                    |                                                                                                                                                     
gTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGtt                                                                                                                                                      >  1:1484101/1‑150 (MQ=255)
  tAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTc                                                                                                                                                    <  2:286489/150‑1 (MQ=255)
    gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCAt                                                                                                                                                  >  1:1423502/1‑150 (MQ=255)
     ggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATg                                                                                                                                                 <  2:323686/150‑1 (MQ=255)
        cTGAAACATTATTCTTCCGCGGGCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGcc                                                                                                                                              <  2:1668842/150‑1 (MQ=255)
          gAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCgg                                                                                                                                            >  1:1388692/1‑150 (MQ=255)
             aCATTATTCTTACGCGGGCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTgg                                                                                                                                         <  1:871365/150‑1 (MQ=255)
              cATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCATGAAACAAGGCCGGttat                                                                                                                                        <  2:299/150‑4 (MQ=255)
               attattCTTACGCGGTCTGGCTGTCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                                                                       <  2:885804/150‑1 (MQ=255)
                 tattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaa                                                                                                                                     >  2:1270024/1‑150 (MQ=255)
                 tattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCATGAAACAAGGCCGGttatggc                                                                                                                                     <  1:1367704/150‑7 (MQ=255)
                   ttCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaaa                                                                                                                                   >  1:868360/1‑150 (MQ=255)
                    tCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                                                                  >  2:803231/1‑150 (MQ=255)
                     cTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaata                                                                                                                                 >  1:1679937/1‑150 (MQ=255)
                     cTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaata                                                                                                                                 <  2:144415/150‑1 (MQ=255)
                       tACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCGTGTTTCATGGCCGGTGGACAaaataaa                                                                                                                               >  2:475461/1‑150 (MQ=255)
                        aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaataaat                                                                                                                              >  1:359011/1‑150 (MQ=255)
                           cgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATCAGTCATTTTTCTTGATTACAGGTGTTACCAACTACCCAACTAACTCGGCGTCCTAACGCACTAGCCATAACCGGCCTTGTTTCATGTCAGGTGGACAAAATAAAttat                                                                                                                           >  2:1479298/1‑148 (MQ=255)
                                 ggCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                                                                     >  1:665152/1‑150 (MQ=255)
                                 ggCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAg                                                                                                                     <  2:240840/150‑1 (MQ=255)
                                     gCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATa                                                                                                                 <  2:1403582/150‑1 (MQ=255)
                                     gCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATa                                                                                                                 >  2:814302/1‑150 (MQ=255)
                                     gCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATa                                                                                                                 <  2:912086/150‑1 (MQ=255)
                                      ccATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATaa                                                                                                                >  1:1664376/1‑150 (MQ=255)
                                       cATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAac                                                                                                               <  2:855807/150‑1 (MQ=255)
                                       cATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAac                                                                                                               <  2:1354620/150‑1 (MQ=255)
                                       cATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGAGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAac                                                                                                               <  2:1347831/150‑1 (MQ=255)
                                       cATTACTCAAAATCCGTTGCAAAAGTAATGAGGCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAac                                                                                                               <  1:1269884/150‑1 (MQ=255)
                                        aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacg                                                                                                              >  1:1499377/1‑150 (MQ=255)
                                          tACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                                                                            <  2:359011/150‑1 (MQ=255)
                                           aCTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                                                                           >  2:1050451/1‑150 (MQ=255)
                                           aCTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                                                                           >  2:140907/1‑150 (MQ=255)
                                           aCTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                                                                           >  1:582711/1‑150 (MQ=255)
                                                aaaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAtg                                                                                                      <  1:658498/150‑1 (MQ=255)
                                                 aaTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAtgt                                                                                                     <  2:1500564/150‑1 (MQ=255)
                                                  aTCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAtgtg                                                                                                    <  1:474280/150‑1 (MQ=255)
                                                   tCCGTTGCAAAAGTAATGAGTGATTTTTCTTGATTACAGGTGTTCCCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTgg                                                                                                   <  2:1151038/150‑1 (MQ=255)
                                                   tCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTgg                                                                                                   <  1:1044645/150‑1 (MQ=255)
                                                    ccGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                                                                  <  1:1050451/150‑1 (MQ=255)
                                                     cGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGac                                                                                                 >  1:1270391/1‑150 (MQ=255)
                                                          cAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACt                                                                                            <  2:1580063/150‑1 (MQ=255)
                                                          cAAAAGTAATGAGTCATTTTTATTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTATGCTTAGCATAAATACGCAATGTGGACACACt                                                                                            >  2:354623/1‑150 (MQ=255)
                                                              aGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgga                                                                                        <  1:1238488/150‑1 (MQ=255)
                                                                tAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTggagg                                                                                      <  1:803231/150‑1 (MQ=255)
                                                                   tGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGAtt                                                                                   <  2:299736/150‑1 (MQ=255)
                                                                      gTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACt                                                                                <  2:151825/150‑1 (MQ=255)
                                                                             ttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTAt                                                                         <  1:1516983/150‑1 (MQ=255)
                                                                               cTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTAttt                                                                       >  1:713016/1‑150 (MQ=255)
                                                                                ttGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTAtttt                                                                      <  2:1270391/150‑1 (MQ=255)
                                                                                ttGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTAtttt                                                                      <  2:1044881/150‑1 (MQ=255)
                                                                                ttGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTAtttt                                                                      <  1:1229549/150‑1 (MQ=255)
                                                                                  gATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTc                                                                    >  2:493382/1‑150 (MQ=255)
                                                                                  caTTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAATCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTc                                                                    <  1:1507603/149‑1 (MQ=255)
                                                                                   aTTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTca                                                                   >  1:402390/1‑150 (MQ=255)
                                                                                    ttACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcag                                                                  <  2:173428/150‑1 (MQ=255)
                                                                                     tACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                                                                 <  2:38291/150‑1 (MQ=255)
                                                                                     tACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagc                                                                 <  1:230072/150‑1 (MQ=255)
                                                                                      aCAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAAGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagcc                                                                >  1:930571/1‑149 (MQ=255)
                                                                                       cAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagcag                                                               <  1:824957/150‑1 (MQ=255)
                                                                                       cAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcagcag                                                               >  1:598981/1‑150 (MQ=255)
                                                                                         ggTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGcc                                                             >  1:657694/1‑150 (MQ=255)
                                                                                         ggTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGcc                                                             >  2:1427544/1‑150 (MQ=255)
                                                                                              tACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttc                                                        >  1:1045328/1‑150 (MQ=255)
                                                                                              tACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttc                                                        >  1:484528/1‑150 (MQ=255)
                                                                                               aCCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttct                                                       >  2:505712/1‑150 (MQ=255)
                                                                                                  aaCTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCa                                                    <  1:1479295/150‑1 (MQ=255)
                                                                                                   aCTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCaa                                                   <  2:769522/150‑1 (MQ=255)
                                                                                                    cTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAag                                                  <  2:665152/150‑1 (MQ=255)
                                                                                                    cTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAag                                                  <  2:627186/150‑1 (MQ=255)
                                                                                                    cTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAag                                                  <  1:1485970/150‑1 (MQ=255)
                                                                                                    cTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAag                                                  <  2:860364/150‑1 (MQ=255)
                                                                                                    cTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAag                                                  <  1:1153338/150‑1 (MQ=255)
                                                                                                        ccAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGtt                                              >  1:1398345/1‑150 (MQ=255)
                                                                                                         cAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTa                                             <  2:713016/150‑1 (MQ=255)
                                                                                                              aactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACAtt                                        >  1:1418598/1‑150 (MQ=255)
                                                                                                               actCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGCCGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACAttt                                       >  1:911512/1‑150 (MQ=255)
                                                                                                               actCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTTGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACAttt                                       <  2:1490070/150‑1 (MQ=255)
                                                                                                                   ggCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAg                                   <  2:657694/150‑1 (MQ=255)
                                                                                                                   ggCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAg                                   >  1:1485412/1‑150 (MQ=255)
                                                                                                                    gCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGc                                  >  1:1472673/1‑150 (MQ=255)
                                                                                                                      gTCCTCACGCACTCTCCATAACCGGGCTTGTTTCATGGCCTGTGGACAAAATAAATTCTGCTTATCATAACGACGCTATTTGGACTCACTTGGATGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCat                                <  1:354623/150‑1 (MQ=255)
                                                                                                                         cTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATg                             >  1:1177043/1‑150 (MQ=255)
                                                                                                                         cTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATg                             >  1:1503103/1‑150 (MQ=255)
                                                                                                                           cACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCg                           >  2:1226608/1‑150 (MQ=255)
                                                                                                                            aCGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGa                          <  1:814302/150‑1 (MQ=255)
                                                                                                                              gCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATc                        <  2:1679937/150‑1 (MQ=255)
                                                                                                                              gCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATc                        <  1:492422/150‑1 (MQ=255)
                                                                                                                               cACTCGCCATAACCGCCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCa                       <  1:1610748/150‑1 (MQ=255)
                                                                                                                                 cTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCAcc                     <  1:1226608/150‑1 (MQ=255)
                                                                                                                                  tCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCt                    <  2:148911/150‑1 (MQ=255)
                                                                                                                                    ttgtccaCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCtta                  >  2:496373/7‑150 (MQ=255)
                                                                                                                                      cATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCttatt                <  2:1045328/150‑1 (MQ=255)
                                                                                                                                        ccaCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtac              >  1:627615/3‑150 (MQ=255)
                                                                                                                                         caCCGGCCATGAAACAAGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtact             >  2:1456418/2‑150 (MQ=255)
                                                                                                                                         aaCCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtact             <  2:1484101/150‑1 (MQ=255)
                                                                                                                                           ccGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATtacaac           >  2:126710/1‑150 (MQ=255)
                                                                                                                                             ggCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTacac         <  2:484528/150‑1 (MQ=255)
                                                                                                                                              gCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATACTACACTa       >  1:208085/1‑150 (MQ=255)
                                                                                                                                                 ttGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAAc     <  2:402390/150‑1 (MQ=255)
                                                                                                                                                    tttCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGc  <  1:493382/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGTTAACATTTGAAGCATATGCGATCACCTTATTACTACACTAACTGC  >  NC_000913/1200765‑1201062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: