Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,205,787 A→T 5.3% pseudogene (57/412 nt) beeE → pseudogene, portal protein family, e14 prophage;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,205,7870AT 5.3% 272.3 / 4.4 94pseudogene (57/412 nt)beeEpseudogene, portal protein family, e14 prophage;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base A (45/44);  new base T (3/2);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

TTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCACC  >  NC_000913/1205639‑1205935
                                                                                                                                                    |                                                                                                                                                    
tttGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCcagcag                                                                                                                                                     <  1:442156/150‑1 (MQ=255)
   gttgttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGc                                                                                                                                                  <  1:531905/150‑1 (MQ=255)
   gttgttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGAGACGCACCGGTGACCACGCCAGCAGAGc                                                                                                                                                  <  2:894714/150‑1 (MQ=255)
     tgttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTg                                                                                                                                                <  1:1581483/150‑1 (MQ=255)
      gttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTgg                                                                                                                                               <  2:499681/150‑1 (MQ=255)
       ttgCCGGGGCGCGGTGCCTGTTCTGGTCGTGGCTGGTGGCGCTATATCTCGACCGTACACAGTCGGCTGTCGGCGGATGTAAATAGTGTTCTTTTCGGGATTATTTGAACGAAAAAGTTACGCACCGGTGACCACGCCAGCAGAGCTGGc                                                                                                                                              <  1:388541/150‑1 (MQ=255)
           cGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCTCTGCTGGCgtgg                                                                                                                                          <  2:526564/150‑4 (MQ=255)
             gggCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGTCCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGc                                                                                                                                        <  2:984636/150‑1 (MQ=255)
              ggCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGcc                                                                                                                                       <  2:1550064/150‑1 (MQ=255)
                 gcTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCAta                                                                                                                                    >  2:1310361/1‑149 (MQ=255)
                   tgtgACTGGTCTGGTCGTGGCGGGAGGCGCGATATCTCGACCGTATACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCgg                                                                                                                                  <  2:301445/150‑1 (MQ=255)
                    gtgCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGGAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCCGTGACCACGCCACCAGAGCTGGCGGCTGCCCACggg                                                                                                                                 >  1:1408258/1‑150 (MQ=255)
                     tgCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGc                                                                                                                                <  1:1259966/150‑1 (MQ=255)
                      gCCTGTTCTGTTAGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCCGTCGGCGGAGGTATATAGTGTTCTTTTCGGGATTATTTCACCGAAAAAGTGACGCACAGGTGACCACGCCAGCAGAGCTGTCGGATGCCATCCGGCt                                                                                                                               >  1:111130/1‑150 (MQ=255)
                      gCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCTTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGCCGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCt                                                                                                                               >  1:212871/1‑150 (MQ=255)
                      gCCTGTTCTGGTCGTGGCTGGGGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCt                                                                                                                               <  2:923870/150‑1 (MQ=255)
                       ccTGTTCTGGTCGTGGCTGGTGGTGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                                                                                                              >  1:811773/1‑150 (MQ=255)
                       ccTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTGAACGAAAAAGTGACGCACCGGTGCCCACGCCAGCAGAGCTGGCGGATGGCATCGGGcag                                                                                                                              >  2:1136688/1‑148 (MQ=255)
                       ccTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCATAGCTGGCGGCAGCCATCGCGCTg                                                                                                                              >  1:387718/1‑150 (MQ=255)
                       ccTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                                                                                                              >  1:975723/1‑150 (MQ=255)
                         tGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCTCTGCTGGCgtgg                                                                                                                                          <  2:1164103/136‑4 (MQ=255)
                         tGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCTCTGCTGGCgtgg                                                                                                                                          >  1:1164103/1‑133 (MQ=255)
                         tGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTc                                                                                                                            >  1:526881/1‑150 (MQ=255)
                                gTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGac                                                                                                                     <  2:1475204/150‑1 (MQ=255)
                                gTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGac                                                                                                                     >  2:1464589/1‑150 (MQ=255)
                                gTCGTGGCTGGTGGCGCGATATCACGACCGTACACAGTCGTCTGTCGGCCGAGGTAAATAGGGATCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACCACAGCAGAGCTGGCGGAGGCCATCGCGCTGTCGTATGaa                                                                                                                     >  1:296498/1‑149 (MQ=255)
                                  cGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGacac                                                                                                                   >  1:1368743/1‑150 (MQ=255)
                                   gTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACAcc                                                                                                                  >  1:468428/1‑150 (MQ=255)
                                      gCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCtat                                                                                                               <  2:683828/150‑1 (MQ=255)
                                      gCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCAATCGGGCTGTCGTATGACACCtat                                                                                                               >  1:1059897/1‑150 (MQ=255)
                                           gggCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCgg                                                                                                          <  2:1513025/149‑1 (MQ=255)
                                            ggCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGa                                                                                                         >  1:1582619/1‑150 (MQ=255)
                                              cgcgATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGaaa                                                                                                       >  2:593492/1‑150 (MQ=255)
                                              cgcgATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTAAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGaaa                                                                                                       >  2:375717/1‑150 (MQ=255)
                                              cgcgATATCTCGACCGTACACAGTCGTCTGTCGGCCGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGAACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGaaa                                                                                                       >  2:1157043/1‑150 (MQ=255)
                                               gcgATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAg                                                                                                      <  1:473579/150‑1 (MQ=255)
                                                 tatatCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCa                                                                                                    <  2:1631396/149‑1 (MQ=255)
                                                 gATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCa                                                                                                    <  2:1590431/150‑1 (MQ=255)
                                                   tatCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGa                                                                                                  <  1:1549553/150‑1 (MQ=255)
                                                    atCTCGACCGTAGACCGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGAt                                                                                                 <  2:1509942/150‑1 (MQ=255)
                                                    atCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGAt                                                                                                 >  2:270546/1‑150 (MQ=255)
                                                     tctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATc                                                                                                <  2:468428/150‑1 (MQ=255)
                                                     tctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATc                                                                                                <  1:438097/150‑1 (MQ=255)
                                                      ctcGACCGTACACAGTCGTCTGTCGGCGGCGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGCCGCACCGGTGCCCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGGCGTATGCCACCTATACCGGAAAGCAGATca                                                                                               >  1:1242240/1‑150 (MQ=255)
                                                      ctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCCTCGGGCTGTCGTATGACACCATTACCGGAAAGCAGATca                                                                                               >  1:375361/1‑150 (MQ=255)
                                                      ctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATca                                                                                               >  1:852093/1‑150 (MQ=255)
                                                      ctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATca                                                                                               >  1:963313/1‑150 (MQ=255)
                                                        cGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCCGCAGAGCGGGCGGATGCCCTCGGGCTGTCGTATGACACCTAGACCGGAAAGCAGCTaagc                                                                                             >  2:1310929/1‑150 (MQ=255)
                                                             gTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCa                                                                                        <  1:473816/150‑1 (MQ=255)
                                                                cacaGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCg                                                                                     <  2:1368743/150‑1 (MQ=255)
                                                                cacaGTCGTCAGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTTTTTCAACGCAAAAGTGACGCACCGGTGCCCACGCCAGAAGAGCTGGCGGTTGGCATCGGGGTGTCGTATGTCTCCTATAGCGGACAGCTGATCAGCAGTGAggg                                                                                     >  2:866828/1‑148 (MQ=255)
                                                                  caGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCggg                                                                                   <  2:139589/150‑1 (MQ=255)
                                                                  caGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCggg                                                                                   >  2:992605/1‑150 (MQ=255)
                                                                           gTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGAc                                                                          <  1:1464589/150‑1 (MQ=255)
                                                                            gcggcggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACt                                                                         <  1:1296196/149‑1 (MQ=255)
                                                                             cggcggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTg                                                                        <  1:164578/150‑1 (MQ=255)
                                                                                cggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcg                                                                     <  1:1489772/150‑1 (MQ=255)
                                                                                 ggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGCcgg                                                                    >  2:760632/1‑150 (MQ=255)
                                                                                 ggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcgg                                                                    <  1:811825/150‑1 (MQ=255)
                                                                                  gAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcggc                                                                   <  2:1243836/150‑1 (MQ=255)
                                                                                   agctAAATAGTCTTCTATTCGGGATTATATCAACGAAAAAGTGACGCACCGGTGACCACTCCAGCAGAGCTGGCGGATTCCATCGCGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcggcg                                                                  <  2:315461/147‑1 (MQ=255)
                                                                                    ggTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcggcgg                                                                 <  2:549158/150‑1 (MQ=255)
                                                                                      tAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGGCTTCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcggcgggt                                                               >  1:174609/1‑148 (MQ=255)
                                                                                        aaTAGTGTTCTTTTCGGGATTCTTTTAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATGGGGCTGTCGTTTGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGtttt                                                             <  2:296498/150‑1 (MQ=255)
                                                                                        aaTAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGtttt                                                             <  2:1083017/150‑1 (MQ=255)
                                                                                        aaTAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGtttt                                                             <  2:1364205/150‑1 (MQ=255)
                                                                                             tgtTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGCTCAGCAGTCCGCGGGCTATGCGACTGACGGCGGTTTTTTccg                                                        >  2:239756/1‑149 (MQ=255)
                                                                                              gtTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTg                                                       >  2:1613446/1‑150 (MQ=255)
                                                                                              gtTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTg                                                       >  1:870083/1‑150 (MQ=255)
                                                                                                    ttCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCag                                                 <  2:967909/150‑1 (MQ=255)
                                                                                                     tCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCaga                                                >  2:805891/1‑150 (MQ=255)
                                                                                                        ggATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTg                                             >  2:1592259/1‑150 (MQ=255)
                                                                                                        ggATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTg                                             >  2:286107/1‑150 (MQ=255)
                                                                                                            tattTCCACGAAAAAGGGAGGCCCCGGTGACCCCGCCAGCAGAGCTGGCGGATGCCATCGGGCTGGCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTgg                                         <  2:320114/150‑1 (MQ=255)
                                                                                                                 cAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCagag                                    >  1:550188/1‑150 (MQ=255)
                                                                                                                 cAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCagag                                    >  2:1074156/1‑150 (MQ=255)
                                                                                                                  aaCGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACTGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAgt                                   >  2:401855/1‑150 (MQ=255)
                                                                                                                    cGAAAAAGTGACGCACCGGTGACCACGCCAGCAGCGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAgtcg                                 >  1:642662/1‑150 (MQ=255)
                                                                                                                        aaaGTGACGCACTGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAgtcggtcg                             <  1:1383773/150‑1 (MQ=255)
                                                                                                                          aGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCggg                           <  2:157020/150‑1 (MQ=255)
                                                                                                                           gggACGCCCCGGGGTCCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGGATGACACCTATACCGGAACGCAGCTCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGGCGGGa                          <  1:46336/148‑1 (MQ=255)
                                                                                                                             gccGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATg                        <  1:872134/148‑1 (MQ=255)
                                                                                                                             gACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATg                        >  1:983355/1‑150 (MQ=255)
                                                                                                                              aCGCACCGGTGACCACGCCGGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGt                       >  2:1656524/1‑150 (MQ=255)
                                                                                                                              aCGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGt                       <  1:1058351/150‑1 (MQ=255)
                                                                                                                                 cACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCGTGCGTATGACACCTATACCGTAAATCAGATCAGCAGTCAGCGGGCTATGCGACTTACGGCGGTTTTATCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGc                    <  1:722515/150‑1 (MQ=255)
                                                                                                                                   ccGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTTAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGccc                  >  1:950099/1‑150 (MQ=255)
                                                                                                                                   ccGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGccc                  >  1:499375/1‑150 (MQ=255)
                                                                                                                                         catccGCCAGCTCTGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAAt            >  2:427014/5‑150 (MQ=255)
                                                                                                                                          atccGCCAGCTCTGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCCGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATc           >  1:1527073/4‑150 (MQ=255)
                                                                                                                                            aCGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTg         >  1:945740/1‑150 (MQ=255)
                                                                                                                                            aCGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTg         <  1:270546/150‑1 (MQ=255)
                                                                                                                                             cGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGt        >  2:43312/1‑150 (MQ=255)
                                                                                                                                             cGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGt        >  1:1238221/1‑150 (MQ=255)
                                                                                                                                              gCCAGCAGCGCGGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTa       <  2:174609/150‑1 (MQ=255)
                                                                                                                                                  gcagAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCAc   <  2:1408258/150‑1 (MQ=255)
                                                                                                                                                   cagAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCAcc  <  1:1613446/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTGTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCACC  >  NC_000913/1205639‑1205935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: