Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,205,789 A→T 5.2% pseudogene (59/412 nt) beeE → pseudogene, portal protein family, e14 prophage;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,205,7890AT 5.2% 256.9 / 4.3 96pseudogene (59/412 nt)beeEpseudogene, portal protein family, e14 prophage;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base A (44/45);  new base T (3/2);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

GTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCACCTGA  >  NC_000913/1205642‑1205938
                                                                                                                                                   |                                                                                                                                                     
gttgttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGc                                                                                                                                                     <  1:531905/150‑1 (MQ=255)
gttgttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGAGACGCACCGGTGACCACGCCAGCAGAGc                                                                                                                                                     <  2:894714/150‑1 (MQ=255)
  tgttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTg                                                                                                                                                   <  1:1581483/150‑1 (MQ=255)
   gttgCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTgg                                                                                                                                                  <  2:499681/150‑1 (MQ=255)
    ttgCCGGGGCGCGGTGCCTGTTCTGGTCGTGGCTGGTGGCGCTATATCTCGACCGTACACAGTCGGCTGTCGGCGGATGTAAATAGTGTTCTTTTCGGGATTATTTGAACGAAAAAGTTACGCACCGGTGACCACGCCAGCAGAGCTGGc                                                                                                                                                 <  1:388541/150‑1 (MQ=255)
        cGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCTCTGCTGGCgtgg                                                                                                                                             <  2:526564/150‑4 (MQ=255)
          gggCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGTCCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGc                                                                                                                                           <  2:984636/150‑1 (MQ=255)
           ggCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGcc                                                                                                                                          <  2:1550064/150‑1 (MQ=255)
              gcTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCAta                                                                                                                                       >  2:1310361/1‑149 (MQ=255)
                tgtgACTGGTCTGGTCGTGGCGGGAGGCGCGATATCTCGACCGTATACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCgg                                                                                                                                     <  2:301445/150‑1 (MQ=255)
                 gtgCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGGAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCCGTGACCACGCCACCAGAGCTGGCGGCTGCCCACggg                                                                                                                                    >  1:1408258/1‑150 (MQ=255)
                  tgCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGc                                                                                                                                   <  1:1259966/150‑1 (MQ=255)
                   gCCTGTTCTGTTAGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCCGTCGGCGGAGGTATATAGTGTTCTTTTCGGGATTATTTCACCGAAAAAGTGACGCACAGGTGACCACGCCAGCAGAGCTGTCGGATGCCATCCGGCt                                                                                                                                  >  1:111130/1‑150 (MQ=255)
                   gCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCTTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGCCGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCt                                                                                                                                  >  1:212871/1‑150 (MQ=255)
                   gCCTGTTCTGGTCGTGGCTGGGGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCt                                                                                                                                  <  2:923870/150‑1 (MQ=255)
                    ccTGTTCTGGTCGTGGCTGGTGGTGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                                                                                                                 >  1:811773/1‑150 (MQ=255)
                    ccTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTGAACGAAAAAGTGACGCACCGGTGCCCACGCCAGCAGAGCTGGCGGATGGCATCGGGcag                                                                                                                                 >  2:1136688/1‑148 (MQ=255)
                    ccTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCATAGCTGGCGGCAGCCATCGCGCTg                                                                                                                                 >  1:387718/1‑150 (MQ=255)
                    ccTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                                                                                                                 >  1:975723/1‑150 (MQ=255)
                      tGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCTCTGCTGGCgtgg                                                                                                                                             >  1:1164103/1‑133 (MQ=255)
                      tGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCTCTGCTGGCgtgg                                                                                                                                             <  2:1164103/136‑4 (MQ=255)
                      tGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTc                                                                                                                               >  1:526881/1‑150 (MQ=255)
                             gTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGac                                                                                                                        <  2:1475204/150‑1 (MQ=255)
                             gTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGac                                                                                                                        >  2:1464589/1‑150 (MQ=255)
                             gTCGTGGCTGGTGGCGCGATATCACGACCGTACACAGTCGTCTGTCGGCCGAGGTAAATAGGGATCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACCACAGCAGAGCTGGCGGAGGCCATCGCGCTGTCGTATGaa                                                                                                                        >  1:296498/1‑149 (MQ=255)
                               cGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGacac                                                                                                                      >  1:1368743/1‑150 (MQ=255)
                                gTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACAcc                                                                                                                     >  1:468428/1‑150 (MQ=255)
                                   gCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCtat                                                                                                                  <  2:683828/150‑1 (MQ=255)
                                   gCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCAATCGGGCTGTCGTATGACACCtat                                                                                                                  >  1:1059897/1‑150 (MQ=255)
                                        gggCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCgg                                                                                                             <  2:1513025/149‑1 (MQ=255)
                                         ggCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGa                                                                                                            >  1:1582619/1‑150 (MQ=255)
                                           cgcgATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGaaa                                                                                                          >  2:593492/1‑150 (MQ=255)
                                           cgcgATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTAAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGaaa                                                                                                          >  2:375717/1‑150 (MQ=255)
                                           cgcgATATCTCGACCGTACACAGTCGTCTGTCGGCCGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGAACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGaaa                                                                                                          >  2:1157043/1‑150 (MQ=255)
                                            gcgATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAg                                                                                                         <  1:473579/150‑1 (MQ=255)
                                              tatatCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCa                                                                                                       <  2:1631396/149‑1 (MQ=255)
                                              gATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCa                                                                                                       <  2:1590431/150‑1 (MQ=255)
                                                tatCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGa                                                                                                     <  1:1549553/150‑1 (MQ=255)
                                                 atCTCGACCGTAGACCGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGAt                                                                                                    <  2:1509942/150‑1 (MQ=255)
                                                 atCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGAt                                                                                                    >  2:270546/1‑150 (MQ=255)
                                                  tctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATc                                                                                                   <  1:438097/150‑1 (MQ=255)
                                                  tctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATc                                                                                                   <  2:468428/150‑1 (MQ=255)
                                                   ctcGACCGTACACAGTCGTCTGTCGGCGGCGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGCCGCACCGGTGCCCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGGCGTATGCCACCTATACCGGAAAGCAGATca                                                                                                  >  1:1242240/1‑150 (MQ=255)
                                                   ctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCCTCGGGCTGTCGTATGACACCATTACCGGAAAGCAGATca                                                                                                  >  1:375361/1‑150 (MQ=255)
                                                   ctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATca                                                                                                  >  1:852093/1‑150 (MQ=255)
                                                   ctcGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATca                                                                                                  >  1:963313/1‑150 (MQ=255)
                                                     cGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCCGCAGAGCGGGCGGATGCCCTCGGGCTGTCGTATGACACCTAGACCGGAAAGCAGCTaagc                                                                                                >  2:1310929/1‑150 (MQ=255)
                                                          gTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCa                                                                                           <  1:473816/150‑1 (MQ=255)
                                                             cacaGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCg                                                                                        <  2:1368743/150‑1 (MQ=255)
                                                             cacaGTCGTCAGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTTTTTCAACGCAAAAGTGACGCACCGGTGCCCACGCCAGAAGAGCTGGCGGTTGGCATCGGGGTGTCGTATGTCTCCTATAGCGGACAGCTGATCAGCAGTGAggg                                                                                        >  2:866828/1‑148 (MQ=255)
                                                               caGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCggg                                                                                      <  2:139589/150‑1 (MQ=255)
                                                               caGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCggg                                                                                      >  2:992605/1‑150 (MQ=255)
                                                                        gTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGAc                                                                             <  1:1464589/150‑1 (MQ=255)
                                                                         gcggcggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACt                                                                            <  1:1296196/149‑1 (MQ=255)
                                                                          cggcggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTg                                                                           <  1:164578/150‑1 (MQ=255)
                                                                             cggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcg                                                                        <  1:1489772/150‑1 (MQ=255)
                                                                              ggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGCcgg                                                                       >  2:760632/1‑150 (MQ=255)
                                                                              ggAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcgg                                                                       <  1:811825/150‑1 (MQ=255)
                                                                               gAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcggc                                                                      <  2:1243836/150‑1 (MQ=255)
                                                                                agctAAATAGTCTTCTATTCGGGATTATATCAACGAAAAAGTGACGCACCGGTGACCACTCCAGCAGAGCTGGCGGATTCCATCGCGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcggcg                                                                     <  2:315461/147‑1 (MQ=255)
                                                                                 ggTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcggcgg                                                                    <  2:549158/150‑1 (MQ=255)
                                                                                   tAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGGCTTCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGAcggcgggt                                                                  >  1:174609/1‑148 (MQ=255)
                                                                                     aaTAGTGTTCTTTTCGGGATTCTTTTAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATGGGGCTGTCGTTTGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGtttt                                                                <  2:296498/150‑1 (MQ=255)
                                                                                     aaTAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGtttt                                                                <  2:1083017/150‑1 (MQ=255)
                                                                                     aaTAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGtttt                                                                <  2:1364205/150‑1 (MQ=255)
                                                                                          tgtTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGCTCAGCAGTCCGCGGGCTATGCGACTGACGGCGGTTTTTTccg                                                           >  2:239756/1‑149 (MQ=255)
                                                                                           gtTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTg                                                          >  2:1613446/1‑150 (MQ=255)
                                                                                           gtTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTg                                                          >  1:870083/1‑150 (MQ=255)
                                                                                                 ttCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCag                                                    <  2:967909/150‑1 (MQ=255)
                                                                                                  tCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCaga                                                   >  2:805891/1‑150 (MQ=255)
                                                                                                     ggATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTg                                                >  2:1592259/1‑150 (MQ=255)
                                                                                                     ggATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTg                                                >  2:286107/1‑150 (MQ=255)
                                                                                                         tattTCCACGAAAAAGGGAGGCCCCGGTGACCCCGCCAGCAGAGCTGGCGGATGCCATCGGGCTGGCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTgg                                            <  2:320114/150‑1 (MQ=255)
                                                                                                              cAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCagag                                       >  1:550188/1‑150 (MQ=255)
                                                                                                              cAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCagag                                       >  2:1074156/1‑150 (MQ=255)
                                                                                                               aaCGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACTGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAgt                                      >  2:401855/1‑150 (MQ=255)
                                                                                                                 cGAAAAAGTGACGCACCGGTGACCACGCCAGCAGCGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAgtcg                                    >  1:642662/1‑150 (MQ=255)
                                                                                                                     aaaGTGACGCACTGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAgtcggtcg                                <  1:1383773/150‑1 (MQ=255)
                                                                                                                       aGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCggg                              <  2:157020/150‑1 (MQ=255)
                                                                                                                        gggACGCCCCGGGGTCCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGGATGACACCTATACCGGAACGCAGCTCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGGCGGGa                             <  1:46336/148‑1 (MQ=255)
                                                                                                                          gccGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATg                           <  1:872134/148‑1 (MQ=255)
                                                                                                                          gACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATg                           >  1:983355/1‑150 (MQ=255)
                                                                                                                           aCGCACCGGTGACCACGCCGGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGt                          >  2:1656524/1‑150 (MQ=255)
                                                                                                                           aCGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGt                          <  1:1058351/150‑1 (MQ=255)
                                                                                                                              cACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCGTGCGTATGACACCTATACCGTAAATCAGATCAGCAGTCAGCGGGCTATGCGACTTACGGCGGTTTTATCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGc                       <  1:722515/150‑1 (MQ=255)
                                                                                                                                ccGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTTAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGccc                     >  1:950099/1‑150 (MQ=255)
                                                                                                                                ccGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGccc                     >  1:499375/1‑150 (MQ=255)
                                                                                                                                      catccGCCAGCTCTGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAAt               >  2:427014/5‑150 (MQ=255)
                                                                                                                                       atccGCCAGCTCTGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCCGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATc              >  1:1527073/4‑150 (MQ=255)
                                                                                                                                         aCGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTg            <  1:270546/150‑1 (MQ=255)
                                                                                                                                         aCGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTg            >  1:945740/1‑150 (MQ=255)
                                                                                                                                          cGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGt           >  2:43312/1‑150 (MQ=255)
                                                                                                                                          cGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGt           >  1:1238221/1‑150 (MQ=255)
                                                                                                                                           gCCAGCAGCGCGGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTa          <  2:174609/150‑1 (MQ=255)
                                                                                                                                               gcagAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCAc      <  2:1408258/150‑1 (MQ=255)
                                                                                                                                                cagAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCAcc     <  1:1613446/150‑1 (MQ=255)
                                                                                                                                                  gagCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCACCTg   >  2:810528/1‑150 (MQ=255)
                                                                                                                                                  gagCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCACCTg   >  2:1495348/1‑150 (MQ=255)
                                                                                                                                                   agCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCACCTGa  >  2:1334016/1‑150 (MQ=255)
                                                                                                                                                   agCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCACCTGa  >  2:805794/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTTGTTGCCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCTATGCGACTGACGGCGGTTTTTTCCTGCGTCAGAGTGCTGGCAGAGTCGGTCGGGATGTTGCCCTGCAATCTGTATCACCTGA  >  NC_000913/1205642‑1205938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: