Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,285,193 T→A 8.3% W19R (TGG→AGG)  narJ → molybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,285,1930TA 8.3% 327.9 / 13.9 117W19R (TGG→AGG) narJmolybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1
Reads supporting (aligned to +/- strand):  ref base T (60/47);  new base A (5/5);  total (65/52)
Fisher's exact test for biased strand distribution p-value = 7.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCG  >  NC_000913/1285044‑1285341
                                                                                                                                                     |                                                                                                                                                     
ggTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTAt                                                                                                                                                       >  1:666585/1‑150 (MQ=255)
ggTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTAt                                                                                                                                                       >  1:590137/1‑150 (MQ=255)
ggTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTAt                                                                                                                                                       >  2:337910/1‑150 (MQ=255)
ggTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTAt                                                                                                                                                       >  2:1593739/1‑150 (MQ=255)
 gTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATg                                                                                                                                                      <  1:1292664/150‑1 (MQ=255)
  tGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATgg                                                                                                                                                     <  1:321065/150‑1 (MQ=255)
   gATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgc                                                                                                                                                    >  1:446266/1‑150 (MQ=255)
   gATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgc                                                                                                                                                    <  1:586834/150‑1 (MQ=255)
   gATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgc                                                                                                                                                    <  2:1654498/150‑1 (MQ=255)
   gATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgc                                                                                                                                                    >  2:1436736/1‑150 (MQ=255)
   gATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGgc                                                                                                                                                    <  1:1596068/150‑1 (MQ=255)
      ggCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCACATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGgcagc                                                                                                                                                 >  1:505851/1‑150 (MQ=255)
         tGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGGCAGCATc                                                                                                                                              <  2:1106310/150‑1 (MQ=255)
         tGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGGCAGCATc                                                                                                                                              <  2:31362/150‑1 (MQ=255)
          gCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATca                                                                                                                                             >  2:41182/1‑150 (MQ=255)
          gCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATca                                                                                                                                             >  2:960582/1‑150 (MQ=255)
          gCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATca                                                                                                                                             <  2:1253548/150‑1 (MQ=255)
          gCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGGCAGCATcc                                                                                                                                             >  2:1264192/1‑149 (MQ=255)
          gCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGGCAGCATcc                                                                                                                                             >  2:471303/1‑149 (MQ=255)
           ccACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATcaa                                                                                                                                            >  2:911191/1‑150 (MQ=255)
            cACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCATAAGGCAGCATccgg                                                                                                                                           <  1:471303/150‑4 (MQ=255)
             aCGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATcaaca                                                                                                                                          <  2:1366806/150‑1 (MQ=255)
             aCGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATcaaca                                                                                                                                          <  1:1638159/150‑1 (MQ=255)
             aCGGCTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATcaaca                                                                                                                                          <  1:677577/150‑1 (MQ=255)
                  tCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAgaga                                                                                                                                     <  1:1395897/150‑1 (MQ=255)
                   cAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGAt                                                                                                                                    <  1:1306363/150‑1 (MQ=255)
                        aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGTCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTg                                                                                                                               <  1:1111501/150‑1 (MQ=255)
                        aCCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTg                                                                                                                               >  2:101208/1‑150 (MQ=255)
                         ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGa                                                                                                                              >  2:586031/1‑150 (MQ=255)
                         ccAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGa                                                                                                                              >  2:410755/1‑150 (MQ=255)
                          cAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAg                                                                                                                             <  1:1520045/150‑1 (MQ=255)
                          cAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAg                                                                                                                             <  1:1596511/150‑1 (MQ=255)
                           aaaTTCAATCTGTTCAACAGCCGTCGTATCGATGCCAACGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTAGAATATCCGGATGCAGCATTATGGCAGCATCAACAAGAGAAGTTTGacg                                                                                                                            >  2:919245/1‑148 (MQ=255)
                            aaTTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGc                                                                                                                           <  1:101208/150‑1 (MQ=255)
                              ttCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGa                                                                                                                         >  1:1087075/1‑150 (MQ=255)
                                cAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGAtt                                                                                                                       >  1:120095/1‑150 (MQ=255)
                                cAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGAtt                                                                                                                       >  2:559835/1‑150 (MQ=255)
                                 aaTCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTg                                                                                                                      >  1:1191377/1‑150 (MQ=255)
                                 aaTCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTg                                                                                                                      >  1:1552085/1‑150 (MQ=255)
                                  aTCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTCTCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGc                                                                                                                     <  1:938445/150‑1 (MQ=255)
                                  aTCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGc                                                                                                                     >  1:1606813/1‑150 (MQ=255)
                                    cTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCcg                                                                                                                   >  2:628052/1‑150 (MQ=255)
                                      tttCAACAGACGTCGTATCGATGCCATCGATGTGACCAGCAAAACTGAGCAGCATCCATTATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGATATGTTTGAGGCGATTGCcgcg                                                                                                                 <  1:575385/149‑1 (MQ=255)
                                      gTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCcgcg                                                                                                                 <  2:665541/150‑1 (MQ=255)
                                          aaCAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGa                                                                                                             >  1:4902/1‑150 (MQ=255)
                                          aaCAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGa                                                                                                             >  2:523611/1‑150 (MQ=255)
                                             aGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGaaaa                                                                                                          >  1:1352002/1‑150 (MQ=255)
                                             aGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCACCTTGAATATCCGCATGCTGCCTTATGGCAGCATCAACAAGAGAAGTTTGAGGCGATTGCCGCGTCGaaaa                                                                                                          >  1:1345246/1‑150 (MQ=255)
                                                      aTCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCaaa                                                                                                 >  2:62556/1‑150 (MQ=255)
                                                      aTCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCaa                                                                                                 >  1:998858/1‑150 (MQ=255)
                                                      aTCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCaa                                                                                                 >  2:336040/1‑150 (MQ=255)
                                                            gCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAgg                                                                                           >  2:1548675/1‑150 (MQ=255)
                                                             ccATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGa                                                                                          <  1:1226370/150‑1 (MQ=255)
                                                             ccATCGATGTGACCAGCAAAACGGAGCCGCATCAATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGa                                                                                          >  1:345001/1‑150 (MQ=255)
                                                                  gaggtgACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGccc                                                                                     <  2:1635815/147‑1 (MQ=255)
                                                                    tgtgACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCAt                                                                                   >  2:730965/1‑150 (MQ=255)
                                                                     gtgACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATg                                                                                  <  1:1548675/150‑1 (MQ=255)
                                                                     gtgACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATg                                                                                  <  1:419451/150‑1 (MQ=255)
                                                                         ccAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCt                                                                             >  2:1146966/1‑150 (MQ=255)
                                                                          cAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTg                                                                            >  2:1201604/1‑150 (MQ=255)
                                                                           aGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTgg                                                                           <  2:1156952/150‑1 (MQ=255)
                                                                           aGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTgg                                                                           >  2:1131794/1‑150 (MQ=255)
                                                                           aGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTgg                                                                           >  1:1552964/1‑150 (MQ=255)
                                                                            tcAAAACGGAGCCGCATCCATGCTCGAACTCGTGATTGTATCGAGTCTCCTTGAATATGCGGATGCTGGCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTAGTAAAATCTGCCAAAAGAGGATGCCCATGCGCTggg                                                                          <  1:112696/149‑1 (MQ=255)
                                                                            gCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCACTggg                                                                          <  2:590137/150‑1 (MQ=255)
                                                                             cAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGATTGAGGCGATTGCCGCGTCGAAAAATCTGCCATAAGAGGATGCCCATGCGCTGGGc                                                                         >  1:717786/1‑150 (MQ=255)
                                                                              aaaaCGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCa                                                                        >  2:1227700/1‑150 (MQ=255)
                                                                              aaaaCGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCa                                                                        <  2:120095/150‑1 (MQ=255)
                                                                              aaaaCGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGGCGAAAAATCTGCCAAAAGAGGATGCCCAGCGCATGGGCa                                                                        <  1:1406535/150‑1 (MQ=255)
                                                                                 aCGGAGCCGCATACATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCAttt                                                                     >  1:11331/1‑150 (MQ=255)
                                                                                    gAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTcc                                                                  >  2:882571/1‑150 (MQ=255)
                                                                                    gAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGATTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGAAGCCCATGCGCTGGGCATTTTcc                                                                  >  1:302782/1‑150 (MQ=255)
                                                                                    gAGCCGCATCCATGATCGAACTAGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTcc                                                                  <  1:1104781/150‑1 (MQ=255)
                                                                                      gCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTg                                                                >  1:778597/1‑150 (MQ=255)
                                                                                      gCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTg                                                                >  2:1685644/1‑150 (MQ=255)
                                                                                      gCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTg                                                                >  2:1302778/1‑150 (MQ=255)
                                                                                             ccATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGAtt                                                         <  2:11331/150‑1 (MQ=255)
                                                                                              cATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGAttt                                                        <  1:1319515/150‑1 (MQ=255)
                                                                                                tGATCGAACTCGTGATTGTTTCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTaa                                                      >  2:818217/1‑150 (MQ=255)
                                                                                                    cGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAacgac                                                  >  2:1042116/1‑150 (MQ=255)
                                                                                                        cTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATg                                              >  1:662944/1‑150 (MQ=255)
                                                                                                             gATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATcc                                         <  2:4902/150‑1 (MQ=255)
                                                                                                                  tATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCg                                    <  1:337910/150‑1 (MQ=255)
                                                                                                                   aTCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGATGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGa                                   >  2:1687773/1‑150 (MQ=255)
                                                                                                                    tCGCGTCTCCTTGAATTTCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGGCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGAt                                  <  2:319993/150‑1 (MQ=255)
                                                                                                                     cgcgTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATg                                 <  2:1191377/150‑1 (MQ=255)
                                                                                                                     cgcgTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATg                                 <  2:1552085/150‑1 (MQ=255)
                                                                                                                          ctcCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAg                            >  2:1030643/1‑150 (MQ=255)
                                                                                                                           tcCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAgg                           <  1:1182686/150‑1 (MQ=255)
                                                                                                                           tcCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAgg                           >  2:387002/1‑150 (MQ=255)
                                                                                                                               tGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTAGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCa                       >  1:1566078/1‑150 (MQ=255)
                                                                                                                                gAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAg                      <  1:336040/150‑1 (MQ=255)
                                                                                                                                  atatCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTa                    >  2:1691484/1‑150 (MQ=255)
                                                                                                                                   cttgttgATGCTGCCATAAGGCAGCATCAACAAGAGAAGTTTGAGGCGATTGCCGCCTCGAAAAATATTCCAAAAGAGGATGCCCAAGCCCTGGGCATTTTCCTGGGCGATTTAACGACGAAGGATCCGCTCGATGCCCAGGCGCAGTAc                   >  2:584749/7‑150 (MQ=255)
                                                                                                                                     tCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAg                 <  1:1436736/150‑1 (MQ=255)
                                                                                                                                     tCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAg                 >  1:1510698/1‑150 (MQ=255)
                                                                                                                                      ccGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGc                >  2:1184911/1‑150 (MQ=255)
                                                                                                                                          aTGCTGCCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAAc            >  1:384700/1‑150 (MQ=255)
                                                                                                                                               gcCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGAACAGCGAACTGTTc       >  2:1570870/1‑150 (MQ=255)
                                                                                                                                               gcCATAAGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTc       >  1:1718587/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGATGGCTGCCACGGTTCAGATACCAAATTCAATCTGTTCAACAGCCGTCGTATCGATGCCATCGATGTGACCAGCAAAACGGAGCCGCATCCATGATCGAACTCGTGATTGTATCGCGTCTCCTTGAATATCCGGATGCTGCCTTATGGCAGCATCAACAAGAGATGTTTGAGGCGATTGCCGCGTCGAAAAATCTGCCAAAAGAGGATGCCCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCG  >  NC_000913/1285044‑1285341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: