Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,286,965 A→G 10.6% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,286,9650AG10.6% 99.3 / 3.7 49intergenic (+439/+101)narI/rttRnitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base A (19/22);  new base G (3/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCG  >  NC_000913/1286817‑1287111
                                                                                                                                                    |                                                                                                                                                  
tcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGAc                                                                                                                                                   >  1:1147272/1‑150 (MQ=255)
   tCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                                                >  2:1102979/1‑150 (MQ=255)
        ccccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGc                                                                                                                                           <  2:473672/150‑1 (MQ=255)
        ccccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGc                                                                                                                                           <  2:1147272/150‑1 (MQ=255)
         cccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGcc                                                                                                                                          <  1:250174/150‑1 (MQ=255)
                gggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAAAGCTCTCTAGCTGCCGCTCGAGGCGAAccat                                                                                                                                   >  1:1709540/1‑148 (MQ=14)
                gggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCAGGGGCTTTTGAAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCATGAGGCAACGCTCTAACGCTGACGCTCGAGGCGAACCtt                                                                                                                                   >  2:1321099/1‑150 (MQ=11)
                  gCAGAATCTTTGATTGCGGCTTCGTTTGTTAATTCCTGGGCTTTTGAAATGGATGGTGGTGGTGTAATGATTACTCAGCGCCGCGCGCTTAGGCGTTCGGGTTGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAg                                                                                                                                 <  2:109220/150‑1 (MQ=14)
                  gCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTACGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGACGAAcataag                                                                                                                                 >  1:995433/1‑145 (MQ=11)
                       ataaTTGATTGCGTATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                            <  2:1602880/150‑1 (MQ=255)
                          tttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGca                                                                                                                         >  2:1577356/1‑149 (MQ=255)
                                        cGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAgt                                                                                                                                           <  1:1330971/118‑2 (MQ=255)
                                        cGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAgt                                                                                                                                           >  2:1330971/1‑117 (MQ=255)
                                        cGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTcccc                                                                                                           <  1:1102979/150‑1 (MQ=255)
                                            tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTATCTCGCAGACCCTACAGACGAACCTTAGTCAAAACTTAACATCCTAcccaaaag                                                                                                       >  2:1663718/1‑145 (MQ=14)
                                                taTTCCGTGTCTTTTGAAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                   <  1:553215/149‑1 (MQ=255)
                                                                 aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCgg                                                                                  >  1:495435/1‑150 (MQ=39)
                                                                 aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCgg                                                                                  >  1:1714493/1‑150 (MQ=39)
                                                                       tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGc                                                                            >  1:487519/1‑150 (MQ=40)
                                                                        ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCgg                                                                                  <  2:757346/143‑1 (MQ=39)
                                                                        ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCgg                                                                                  >  1:757346/1‑143 (MQ=39)
                                                                                    acgcttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                               <  2:1182910/146‑1 (MQ=255)
                                                                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAg                                                                                                                                            <  1:1097881/66‑1 (MQ=35)
                                                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                                   <  2:487519/150‑1 (MQ=255)
                                                                                                    gcgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTTGAAAGTTAAGGGACATTTCaaaataaa                                               >  2:1391813/1‑145 (MQ=11)
                                                                                                      gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtgg                                             >  2:634619/1‑150 (MQ=255)
                                                                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGTAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                                          <  1:1103924/134‑1 (MQ=255)
                                                                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGTAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                                          >  2:1103924/1‑134 (MQ=255)
                                                                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtgg                                          >  2:354601/1‑150 (MQ=255)
                                                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                                         <  1:401607/150‑1 (MQ=255)
                                                                                                           tCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCAACCTTACCCCCATGGGCAGAATATTTTATTGCGGATTCGGTTGTAAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                        >  2:1256739/1‑150 (MQ=11)
                                                                                                           tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                        <  2:495435/150‑1 (MQ=255)
                                                                                                           tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                        <  2:1714493/150‑1 (MQ=255)
                                                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATg                                                                                                      >  1:1203005/1‑86 (MQ=255)
                                                                                                              ctcTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                     <  2:379876/148‑1 (MQ=255)
                                                                                                              cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                     <  2:979003/150‑1 (MQ=255)
                                                                                                                cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGa                                   >  2:1218402/1‑150 (MQ=255)
                                                                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGc                                                                                                                                                 <  1:479834/36‑1 (MQ=40)
                                                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACGTTAGTCGAAGCTTCTCAGCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGGTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                          >  1:265439/1‑150 (MQ=255)
                                                                                                                                 cGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTg                  <  1:354601/150‑1 (MQ=255)
                                                                                                                                  ggCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTccc                                                                                                            >  2:216199/1‑59 (MQ=255)
                                                                                                                                  ggCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTccc                                                                                                            <  1:216199/59‑1 (MQ=255)
                                                                                                                                   gCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtgg                                             >  2:299178/1‑121 (MQ=255)
                                                                                                                                   gCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtgg                                             <  1:299178/121‑1 (MQ=255)
                                                                                                                                    cAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgc               >  1:148445/1‑150 (MQ=255)
                                                                                                                                          tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg         <  1:634619/150‑1 (MQ=255)
                                                                                                                                                gCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCgttg                                                                          <  2:502928/79‑4 (MQ=255)
                                                                                                                                                gCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCgttg                                                                          >  1:502928/1‑76 (MQ=255)
                                                                                                                                                 cTGACGCTCGAGCCTAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCg  <  2:265439/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCG  >  NC_000913/1286817‑1287111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑