Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,292,404 A→C 5.1% intergenic (+39/+105) galU → / ← hns glucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,292,4040AC 5.1% 311.2 / 6.1 99intergenic (+39/+105)galU/hnsglucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS
Reads supporting (aligned to +/- strand):  ref base A (49/45);  new base C (2/3);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 102 total aligned reads displayed.

ATTAGGTTACATGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCAT  >  NC_000913/1292263‑1292552
                                                                                                                                             |                                                                                                                                                    
aTTAGGTTACATGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGcc                                                                                                                                              <  2:1177994/150‑1 (MQ=255)
     gTTACATGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGtttt                                                                                                                                         >  1:1042538/1‑150 (MQ=255)
      ttACATGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTCGATTGCTCAACGCCGtttcg                                                                                                                                        <  2:1637275/150‑3 (MQ=255)
      ttACATGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTCGATTGCTCAACGCCGtttcg                                                                                                                                        <  1:1413733/150‑3 (MQ=255)
        aCATGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAATAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGttttttt                                                                                                                                      <  2:723904/150‑1 (MQ=255)
          aTGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAATCCTGGCGTGAAGAAGAGATGGGAATTAAGAAGTAACATCCGTATCGGTGTTATCAACGAAACGGCGTTGAGCAATCGACGCCGTATTTTtat                                                                                                                                    >  1:1104719/1‑150 (MQ=255)
           tGCAGGCCTTCGTTGAATACGGTATTCTTCATAACAACATTGGCACGGAATTTAAAGACTTGCTTGAAGAAGAGATGGGCATTAAGAAGTAACAAACGTATCGGTTTTATACACTAAACTTAGTTTAGAAATCGACGCCGTTATTTtata                                                                                                                                   >  1:1015577/1‑150 (MQ=255)
           tGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTCGATTGCTCAACGCCGtttcgtggat                                                                                                                                   <  1:149858/150‑8 (MQ=255)
           tGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGCCGTTGAGCAATCGACGCCGTTTTTTtata                                                                                                                                   >  1:841333/1‑150 (MQ=255)
             cAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGc                                                                                                                                 >  2:392798/1‑150 (MQ=255)
              aGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGct                                                                                                                                <  1:696086/150‑1 (MQ=255)
               ggCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGctt                                                                                                                               <  2:678643/150‑1 (MQ=255)
               ggCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGctt                                                                                                                               >  2:1645677/1‑150 (MQ=255)
                 ccTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGcttat                                                                                                                             >  1:572027/1‑150 (MQ=255)
                 ccTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCAGTTTTTTTATAGcttat                                                                                                                             >  1:1299472/1‑150 (MQ=255)
                  cTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGcttatt                                                                                                                            >  2:599911/1‑150 (MQ=255)
                       ttGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGcttattcttat                                                                                                                       >  2:311165/1‑150 (MQ=255)
                       ttGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGcttattcttat                                                                                                                       <  2:547726/150‑1 (MQ=255)
                         gAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCttatta                                                                                                                     <  1:1106627/150‑1 (MQ=255)
                         gAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCttatta                                                                                                                     >  1:270129/1‑150 (MQ=255)
                           aTACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTaaa                                                                                                                   >  1:810931/1‑150 (MQ=255)
                            tACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGAATCGGTGTTATCCACGAAACGTCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAAt                                                                                                                  <  1:95984/150‑1 (MQ=255)
                                 tATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCt                                                                                                             <  2:1487842/150‑1 (MQ=255)
                                     cGTCATTACACCCTTGGCACGGAATTTAAAGCCAGTCTTGAAGAAGAGATGGCCAATAAGAAGTAACATCCGAATCGGTGTAATCCACGAAACCCCGTTGAGCAAACGACACCGTATTTTTATAGCTTATTCTTATTAAAATGTCTTaca                                                                                                         >  1:1098928/1‑148 (MQ=255)
                                     cGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGAATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGACTTaaa                                                                                                         >  2:302252/1‑150 (MQ=255)
                                        cATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCg                                                                                                      >  2:559154/1‑150 (MQ=255)
                                        cATAACACCCTTGGCACGGAATTAAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCg                                                                                                      <  2:1375708/150‑1 (MQ=255)
                                         aTAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCgg                                                                                                     <  2:1060258/150‑1 (MQ=255)
                                          tAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGa                                                                                                    <  2:944937/150‑1 (MQ=255)
                                           aaCACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGAc                                                                                                   >  2:704557/1‑150 (MQ=255)
                                           aaCACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGAc                                                                                                   >  2:441230/1‑150 (MQ=255)
                                             cacCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACaa                                                                                                 >  2:1248393/1‑150 (MQ=255)
                                             cacCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACaa                                                                                                 <  2:572027/150‑1 (MQ=255)
                                              acCCTTGGCTCGTAATTTAAAGCCTGGCTTGAAGAAGAGAAGGGCATTAAGAAGTAACATCCGTATCGGTTTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAAt                                                                                                <  2:347084/150‑1 (MQ=255)
                                              acCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAAt                                                                                                >  1:1136960/1‑150 (MQ=255)
                                              acCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATa                                                                                               >  2:432455/1‑150 (MQ=255)
                                                ccTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATaa                                                                                              <  1:666327/150‑1 (MQ=255)
                                                   tGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATaaaaa                                                                                           <  2:1168066/150‑1 (MQ=255)
                                                      cACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTAGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATc                                                                                        <  1:269201/150‑1 (MQ=255)
                                                        cGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATccc                                                                                      <  1:1697011/150‑1 (MQ=255)
                                                         gtaaTTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACCTCCGAATCGGTTTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCccg                                                                                     <  1:819466/148‑1 (MQ=255)
                                                         ggAAGTTCACGCCTGGCTTGAAGACTCGATGCGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAATCCGGACAATAAAAAATCccg                                                                                     <  2:153933/150‑1 (MQ=255)
                                                                aaaGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTgg                                                                              >  1:241747/1‑150 (MQ=255)
                                                                aaaGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTgg                                                                              >  1:1421434/1‑150 (MQ=255)
                                                                  aGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCg                                                                            <  1:392798/150‑1 (MQ=255)
                                                                   gcctggcctgAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCgg                                                                           >  2:1186416/1‑150 (MQ=255)
                                                                    ccTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACAGGACAATAAAAAATCCCGCCGCTGGCggg                                                                          >  1:738110/1‑150 (MQ=255)
                                                                     cTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGa                                                                         >  2:896731/1‑150 (MQ=255)
                                                                       ggCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTATTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAtt                                                                       >  1:1039682/1‑150 (MQ=255)
                                                                         cTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAtttt                                                                     >  2:19331/1‑150 (MQ=255)
                                                                         cTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAtttt                                                                     <  1:572913/150‑1 (MQ=255)
                                                                          ctGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTa                                                                    <  1:1186416/149‑1 (MQ=255)
                                                                           tGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTaa                                                                   <  1:28409/150‑1 (MQ=255)
                                                                            gaagaaGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCAGCGGCGGGAtttttta                                                                  <  2:1136960/150‑4 (MQ=255)
                                                                             aagaagCGATCGGCATTAAGAAGTAACATCCGTATCGGTGTTAACCACGAAACCGCGTTGAGCAATCGACGCCGTTTTTTTAAAGCTTATACTTATTAAATTGTCTTAAACCGGACCATAAAAAATCCCGCCGCTGGCGGGATTTTAAGc                                                                 <  1:1171628/150‑1 (MQ=255)
                                                                              agaagaTATGGGCATTAAGTAGTAACATCCGTATCGGTGTTATCCAAGAAACGGCGTTTAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCa                                                                <  1:336349/150‑1 (MQ=255)
                                                                              agaagaGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCa                                                                >  1:625354/1‑150 (MQ=255)
                                                                                aagaGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTATTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGTGATTTTAAGCaat                                                              >  1:450812/1‑149 (MQ=255)
                                                                                 agagATGGGCATTAAGAAGTAACATCCGTATTGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGt                                                             >  2:1336837/1‑150 (MQ=255)
                                                                                   agaTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAg                                                                  <  1:1422465/143‑1 (MQ=255)
                                                                                   agaTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAg                                                                  >  2:1422465/1‑143 (MQ=255)
                                                                                    caTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCa                                                          <  1:340917/149‑1 (MQ=255)
                                                                                      tGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAAt                                                        <  1:19331/150‑1 (MQ=255)
                                                                                       tggCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATc                                                       <  1:1459451/149‑1 (MQ=255)
                                                                                        ggCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTGTAAGCAAGTGCAATCt                                                      >  2:34385/1‑150 (MQ=255)
                                                                                              aagaagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACaaaa                                                <  1:355474/150‑1 (MQ=255)
                                                                                                gaagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGa                                              >  2:1677185/1‑150 (MQ=255)
                                                                                                 aagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGat                                             <  2:1680021/150‑1 (MQ=255)
                                                                                                        aTCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCt                                      <  1:1645677/150‑1 (MQ=255)
                                                                                                        aTCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCt                                      <  2:270129/150‑1 (MQ=255)
                                                                                                          ccGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTg                                    <  2:1421434/150‑1 (MQ=255)
                                                                                                          acGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTg                                    <  2:1219160/149‑1 (MQ=255)
                                                                                                             tATCTGTTTTATCCACGAAACTGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATc                                 <  1:311165/150‑1 (MQ=255)
                                                                                                                cGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAgg                              <  2:625354/150‑1 (MQ=255)
                                                                                                                 ggTTTTATCCAAGAAACGGCGTTGAGCTATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGa                             <  2:235243/150‑1 (MQ=255)
                                                                                                                  gtgtTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGaa                            >  1:465939/1‑150 (MQ=255)
                                                                                                                     ttATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtc                         >  2:134611/1‑150 (MQ=255)
                                                                                                                      tATCCACGAAACGGCGTTGAGCAATCGACGCCCTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcg                        >  2:1282414/1‑150 (MQ=255)
                                                                                                                       ttCCAAGAAACGGCGTTGAGAAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGTCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcgt                       <  2:841333/149‑1 (MQ=255)
                                                                                                                         ccACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcgtcg                     >  2:1701374/1‑150 (MQ=255)
                                                                                                                         ccACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcgtcg                     >  2:1323149/1‑150 (MQ=255)
                                                                                                                          cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                    >  1:86361/1‑150 (MQ=255)
                                                                                                                          cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                    >  1:413697/1‑150 (MQ=255)
                                                                                                                           aCGAAACGGCGTTGATCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAg                   <  2:1253049/150‑1 (MQ=255)
                                                                                                                           aCGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAg                   <  2:1634177/150‑1 (MQ=255)
                                                                                                                           aCGAAACGGAGTTTAGCAATCGACGCCCAATTATAATAGCTAATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCACTGGCGCGATTTAAAGCAAGTGCAAACTACAAAACATTATTGCTTGAACAGGAAATcgacgaa                   >  1:1374542/1‑149 (MQ=255)
                                                                                                                            cGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAgg                  >  1:842333/1‑150 (MQ=255)
                                                                                                                            cGAAACGGAGTTGAGAAATCGACTCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAgg                  <  2:450812/150‑1 (MQ=255)
                                                                                                                            aaaaaCGGCGTCGATTGCTCAACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAgg                  >  1:1274359/3‑150 (MQ=255)
                                                                                                                               aaCGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGAt               >  2:1300947/1‑150 (MQ=255)
                                                                                                                                aCGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGAtt              >  1:781834/1‑150 (MQ=255)
                                                                                                                                 cGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCt                                      >  1:1216827/1‑125 (MQ=255)
                                                                                                                                 cGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCt                                      <  2:1216827/125‑1 (MQ=255)
                                                                                                                                   gCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTAc           >  2:1556905/1‑150 (MQ=255)
                                                                                                                                     gTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGAAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGTATTTACCt         >  1:1056392/1‑150 (MQ=255)
                                                                                                                                         aGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCt     >  2:1090670/1‑150 (MQ=255)
                                                                                                                                          gCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTc    >  2:696832/1‑150 (MQ=255)
                                                                                                                                            aaTCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCTGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGTGATTTACCTTGCTCAt  >  1:251787/1‑150 (MQ=255)
                                                                                                                                            aaTCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAATATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCaa  >  1:1159001/1‑149 (MQ=255)
                                                                                                                                            aaTCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCAt  >  1:1153045/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
ATTAGGTTACATGCAGGCCTTCGTTGAATACGGTATTCGTCATAACACCCTTGGCACGGAATTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCAT  >  NC_000913/1292263‑1292552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: