Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,306,788 T→C 13.0% intergenic (+20/+33) oppF → / ← yciU oligopeptide ABC transporter ATPase/UPF0263 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,306,7880TC13.0% 239.0 / 26.3 101intergenic (+20/+33)oppF/yciUoligopeptide ABC transporter ATPase/UPF0263 family protein
Reads supporting (aligned to +/- strand):  ref base T (37/49);  new base C (4/9);  total (42/59)
Fisher's exact test for biased strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

Only 100 of 102 total aligned reads displayed.

CCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTT  >  NC_000913/1306643‑1306936
                                                                                                                                                 |                                                                                                                                                    
ccGCCTTCCGGTTGTGTTTTCCGTTCCCGTTGCCCGATTGCCGCTCCAGAGTGCGCCAAAACAAGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGACTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTc                                                                                                                                                  <  1:87985/150‑1 (MQ=255)
ccGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGGGTCTTGCCTGACAGTCGATCCGCTTTAAAACCAATAAGGGCTGACAGTTGTc                                                                                                                                                  <  1:83558/150‑1 (MQ=255)
ccGCCTTCCGGTTGTGGTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTc                                                                                                                                                  <  1:1488290/150‑1 (MQ=255)
   ccTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGc                                                                                                                                               <  1:591068/150‑1 (MQ=255)
     ttCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCGTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGCCAGATGTCAGccc                                                                                                                                             >  2:1021758/1‑150 (MQ=255)
      tCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGTTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCt                                                                                                                                            <  2:1173742/150‑1 (MQ=255)
      tCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCt                                                                                                                                            <  1:338618/150‑1 (MQ=255)
      tCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCt                                                                                                                                            <  1:159732/150‑1 (MQ=255)
        cGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCtta                                                                                                                                          <  1:1429787/150‑2 (MQ=255)
         ggTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCtttt                                                                                                                                         >  2:1207295/1‑150 (MQ=255)
         ggTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCttat                                                                                                                                         <  1:604466/150‑3 (MQ=255)
          gTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAACCACGTCCTGTTCTGGAGGGGAGATTCAGACACTCCGTTTCTTGCCTGAAAGGCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCttatt                                                                                                                                        <  1:587682/150‑1 (MQ=255)
           ttGTGTTTTCCGTACCCGTTGCCCGATTGCCGGGCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAAGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCttattg                                                                                                                                       <  1:1477433/150‑2 (MQ=255)
           ttGTGTGTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCttattg                                                                                                                                       <  2:506272/150‑2 (MQ=255)
             ttggtttCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGTGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCAc                                                                                                                                     <  1:180032/146‑1 (MQ=255)
             gtgtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCttattgtt                                                                                                                                     <  1:897415/150‑4 (MQ=255)
               gtctaccGTACCCGTTGCCCGATTGCCGGTCCAGAGTCCGCCAAAACACGTCCTGTTCTGGATGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTTACGc                                                                                                                                   <  2:1616769/145‑1 (MQ=255)
               gtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGc                                                                                                                                   <  1:702298/150‑1 (MQ=255)
                   tCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTaaa                                                                                                                               >  2:1546020/1‑150 (MQ=255)
                   tCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTaaa                                                                                                                               >  2:276503/1‑150 (MQ=255)
                     cGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTATTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAg                                                                                                                             >  1:1528869/1‑150 (MQ=255)
                        aCCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGa                                                                                                                          >  2:611144/1‑150 (MQ=255)
                               gCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttattta                                                                                                                   >  1:382997/1‑150 (MQ=255)
                                cccGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATGCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttatttat                                                                                                                  >  2:1628503/1‑150 (MQ=255)
                                cccGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAATCGAttatttat                                                                                                                  >  2:1286196/1‑150 (MQ=255)
                                cccGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttatttat                                                                                                                  >  1:1389072/1‑150 (MQ=255)
                                 ccGACTGCCGGGCCAGAGGTCGCCAAACCACGACCTGTTCTGGAGGGTAGTTTCAGACACTCCGTTTCTTGCCGGAAAGTCGATCCGCTTTAAAAACAATAATGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATtatttatt                                                                                                                 <  1:111503/150‑1 (MQ=255)
                                   gATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTcc                                                                                                               <  1:386269/150‑1 (MQ=255)
                                    aTTGCCGGTGCAGAGTGCGACAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTccc                                                                                                              <  1:325457/150‑1 (MQ=255)
                                       gCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATACGGGCTGACAGTGGTCAGCCCTTTGTCCCGCTAAGAGCGATTATTTTTTCCCGcc                                                                                                           >  2:230370/1‑150 (MQ=255)
                                         cGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAg                                                                                                         <  2:763473/150‑1 (MQ=255)
                                          ggTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAga                                                                                                        >  1:3652/1‑150 (MQ=255)
                                           gTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgat                                                                                                       <  1:594429/150‑1 (MQ=255)
                                            tCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatg                                                                                                      <  1:1478950/150‑1 (MQ=255)
                                             ccAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatga                                                                                                     <  1:1136178/150‑1 (MQ=255)
                                             ccAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatga                                                                                                     <  1:1587724/150‑1 (MQ=255)
                                              cAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatgat                                                                                                    <  2:382997/150‑1 (MQ=255)
                                               agagTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGata                                                                                                   <  2:1405192/150‑1 (MQ=255)
                                                 agTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGGTTCAGACACTCCGTTTCTTGCCGGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATACCCGCCAGATGATATg                                                                                                 <  1:1550048/150‑1 (MQ=255)
                                                      gcCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaaa                                                                                            >  1:434643/1‑150 (MQ=255)
                                                      gcCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGAGGATATGGCaaa                                                                                            >  2:1365429/1‑150 (MQ=255)
                                                       ccAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCATTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAg                                                                                           >  1:142336/1‑150 (MQ=255)
                                                       ccAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTAAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAg                                                                                           >  1:235409/1‑150 (MQ=255)
                                                         aaaaCACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGCTCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGtt                                                                                         >  1:224390/1‑150 (MQ=255)
                                                         aaaaCACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGtt                                                                                         <  2:758316/150‑1 (MQ=255)
                                                          aaaCACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATTGCAAAGttt                                                                                        >  2:487226/1‑150 (MQ=255)
                                                           aaCACGTCCTGTTCTGGAGGTGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACATTAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtg                                                                                       <  1:1561670/150‑1 (MQ=255)
                                                            acacGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgt                                                                                      <  2:918318/150‑1 (MQ=255)
                                                             cagggccTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGTGCTGTCAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgtg                                                                                     <  2:890970/145‑1 (MQ=255)
                                                                     gTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttttt                                                                             <  1:1428370/150‑1 (MQ=255)
                                                                     gTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttttt                                                                             >  1:166586/1‑150 (MQ=255)
                                                                       tCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcg                                                                           >  1:727983/1‑150 (MQ=255)
                                                                       tCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcg                                                                           <  2:3652/150‑1 (MQ=255)
                                                                          gaaacggAGTGTCTGAAACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAATCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCgg                                                                        >  2:1067742/6‑150 (MQ=255)
                                                                             ggggAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCAt                                                                     >  2:1171834/1‑150 (MQ=255)
                                                                                gagaTTCAGACACTCCGTTTCTTGCCTGAAAGTCGTTCCGCTTTAAAAACAATAAGGGCTGACTGTTTTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAAc                                                                  <  1:1021758/150‑1 (MQ=255)
                                                                                gAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAAc                                                                  <  1:276503/150‑1 (MQ=255)
                                                                                gAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAAc                                                                  <  1:1546020/150‑1 (MQ=255)
                                                                                 aGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCACTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACa                                                                 >  2:903519/1‑150 (MQ=255)
                                                                                    ttCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGaa                                                              >  2:1293807/1‑150 (MQ=255)
                                                                                     tCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAAt                                                             <  1:1688337/150‑1 (MQ=255)
                                                                                     acAGACACACCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCATTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATCATATGGCAAAGTTTGTGATCTTTTTCGAGCCATAACAGAAt                                                             >  2:289220/2‑150 (MQ=255)
                                                                                      aaGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATg                                                            <  1:1567979/149‑1 (MQ=255)
                                                                                        gACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTTAAAACAATAAGGGCTGACAGTTGGCAGCCCGTTTTCACGCTAAATGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCg                                                          <  1:248461/150‑1 (MQ=255)
                                                                                        gACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCg                                                          <  1:1447509/150‑1 (MQ=255)
                                                                                        gACACTCAGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCg                                                          >  2:1210199/1‑150 (MQ=255)
                                                                                         acacTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCgg                                                         <  1:1402840/150‑1 (MQ=255)
                                                                                          cacTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCggg                                                        <  2:1700391/150‑1 (MQ=255)
                                                                                           aatCCGTTTCTTGCATGTAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGc                                                       <  2:759993/148‑1 (MQ=255)
                                                                                                  ttCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACa                                                <  2:1023955/150‑1 (MQ=255)
                                                                                                    cTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACTGAATGCGGGCAAAAACATc                                              >  2:214533/1‑150 (MQ=255)
                                                                                                     ttGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATTGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCg                                             <  1:1288074/150‑1 (MQ=255)
                                                                                                         cTGAAAGTCGATCCGCTTTAAAAACAATCAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTACAAGAGATTATTTAATCCCGCCAGATGATATGGCATAGTTTGTGATCTTTTTCGCGGCATAACAGTATGCGGGCAAAAACATCGTTgt                                         <  1:113373/150‑2 (MQ=255)
                                                                                                          tGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGc                                                       >  1:1198384/1‑135 (MQ=255)
                                                                                                          tGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGc                                                       <  2:1198384/135‑1 (MQ=255)
                                                                                                          tGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGAt                                        >  2:701906/1‑150 (MQ=255)
                                                                                                             aaGTCGATCCGCTTTAATAACAATAAGTGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAACCGAGTATTTATTCCCGCCAGATGATATGGCAAAGTTTGGGATCTTTATCGCGGCATAACAGAATGCGGGCAAAAACATCGGTGATTTc                                     <  1:318212/150‑1 (MQ=255)
                                                                                                               gTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGATTGCGGGCAAAAACATCGTTGATTTCAc                                   >  2:78014/1‑150 (MQ=255)
                                                                                                               gTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCAc                                   <  2:166586/150‑1 (MQ=255)
                                                                                                               gTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCAc                                   <  2:1528869/150‑1 (MQ=255)
                                                                                                                tCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCAcc                                  <  1:611144/150‑1 (MQ=255)
                                                                                                                 agATCCGCTTTAAAAACAATAAGGTCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCa                                 <  1:1379663/149‑1 (MQ=255)
                                                                                                                      cGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTc                            <  2:1389072/150‑1 (MQ=255)
                                                                                                                         tttAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACt                         <  2:224390/150‑1 (MQ=255)
                                                                                                                           tAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGt                       >  1:1283669/1‑150 (MQ=255)
                                                                                                                            aaaaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTc                      >  1:1711675/1‑150 (MQ=255)
                                                                                                                             aaaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTcc                     >  1:122713/1‑150 (MQ=255)
                                                                                                                                 gaaaaaGGGCAGACAACTGTCAGCCATTTTTAACCCTAAAAGCGATTAATTAATCCCGCCAGATGAAAAGGCAAACTTAGAGATCTTTTTCGCGGCATAACAGAATGCCGGCAAAAAAATCGTTTAATTCACCAACTTCACTCTCCGCCa                 >  2:441836/5‑150 (MQ=255)
                                                                                                                                  aaTAAGGGCTGACAGTTGTCAGCCCTTTTTCAAGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAg                <  1:872135/150‑1 (MQ=255)
                                                                                                                                   aaaaGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGa               >  1:500462/3‑150 (MQ=255)
                                                                                                                                     ttcggatGACAGTTGTCAGACCTTTTTCACGCTAAAAGCTATTATTTATTCCCGACAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAATACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAcc             <  1:289220/144‑1 (MQ=255)
                                                                                                                                     aaGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAcc             >  2:49756/1‑150 (MQ=255)
                                                                                                                                     aaGGGCTGACAACTGTCAGACCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCCCCAGAcc             >  2:1315614/1‑150 (MQ=255)
                                                                                                                                      aGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAccg            >  2:452822/1‑149 (MQ=255)
                                                                                                                                           tGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCAGACCAATcacc      <  2:727983/150‑1 (MQ=255)
                                                                                                                                           tGACAGGTGGCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATcac       <  2:1384571/150‑1 (MQ=255)
                                                                                                                                            gACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATcacc      >  2:1596078/1‑150 (MQ=255)
                                                                                                                                             aCAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAcc             >  2:803080/1‑142 (MQ=255)
                                                                                                                                             aCAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAcc             <  1:803080/142‑1 (MQ=255)
                                                                                                                                                cttGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATACCCGGCAGATGATATGGCAAACTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATAGTTGATTTCACAATCTTCACTGTACGCCAGACCAATcaccacat  >  1:72212/2‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTT  >  NC_000913/1306643‑1306936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: