Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,311,286 G→T 5.8% E66D (GAG→GAT) ‡ tonB → membrane spanning protein in TonB‑ExbB‑ExbD transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,311,2860GT 5.8% 269.0 / 5.2 86E66D (GAG→GAT) ‡tonBmembrane spanning protein in TonB‑ExbB‑ExbD transport complex
Reads supporting (aligned to +/- strand):  ref base G (37/44);  new base T (2/3);  total (39/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

CTGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAAC  >  NC_000913/1311139‑1311434
                                                                                                                                                   |                                                                                                                                                    
cTGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCGGCGCAGCCGATTTCTGTCACGATGGTTACGCCTACTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGcc                                                                                                                                                    <  1:324034/150‑1 (MQ=255)
 tGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCCCAAGCCGTTCAGCCGCCCCCGGAGCCg                                                                                                                                                   >  2:288464/1‑150 (MQ=255)
  gCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCgg                                                                                                                                                  >  2:876458/1‑150 (MQ=255)
   cATTCATGGTGCTTTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCggt                                                                                                                                                 <  2:1309200/150‑1 (MQ=255)
   cATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACCAGCCGTTCAGCCGCCACCGGAGCCggt                                                                                                                                                 >  2:138328/1‑150 (MQ=255)
   cATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCggt                                                                                                                                                 <  1:862536/150‑1 (MQ=255)
   cATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCggt                                                                                                                                                 <  2:103008/150‑1 (MQ=255)
      tCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggc                                                                                                                                              <  2:854704/150‑2 (MQ=255)
        aTGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTag                                                                                                                                            <  1:967694/150‑1 (MQ=255)
        aTGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTag                                                                                                                                            <  1:66657/150‑1 (MQ=255)
         tGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggcggc                                                                                                                                           <  1:424013/150‑5 (MQ=255)
             gCTGTTGTCGCGGGGCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTGCAGCCGCCACCGGAGCCGGTGGTAGAGCCa                                                                                                                                       <  1:288464/150‑1 (MQ=255)
              cTGTTGTCGCGGGTCTGCTCTATACCTCCGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGAGGTAGAGCCAg                                                                                                                                      >  1:273210/1‑150 (MQ=255)
                gttgtGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGaa                                                                                                                                    <  1:495322/150‑1 (MQ=255)
                 gtgtgGCGGGTCTGCGCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAAc                                                                                                                                   <  1:136013/149‑1 (MQ=255)
                  tgtgGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAAcc                                                                                                                                  <  2:404560/150‑1 (MQ=255)
                  tgtgGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAAcc                                                                                                                                  <  1:400008/150‑1 (MQ=255)
                   gtgGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACCAGCCGTTCAGCCGCCCCCGGCGCCGGTGGTAGCGCCAGAACCg                                                                                                                                 >  2:1241930/1‑150 (MQ=255)
                   gtgGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCg                                                                                                                                 >  2:1470037/1‑150 (MQ=255)
                      gCGGGTCTGCGCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCACCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGaa                                                                                                                              <  2:434996/150‑1 (MQ=255)
                          gTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTg                                                                                                                          >  1:104729/1‑150 (MQ=255)
                            cTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAg                                                                                                                        <  1:1591047/150‑1 (MQ=255)
                            cTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAg                                                                                                                        >  1:1424461/1‑150 (MQ=255)
                             tGCTCTATACCTCGGTACATCAGGGTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGc                                                                                                                       <  1:1214211/150‑1 (MQ=255)
                                   ataCCTCGTTACTTCAGTTTATTGAACTTCCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCAGTTCAGCGGCCTCCGGAGCCGGTGGTAGATCCAGAACCGGAACCTGAGCCGATcc                                                                                                                 <  2:665352/150‑1 (MQ=255)
                                   ataCCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCAGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATcc                                                                                                                 <  2:81790/150‑1 (MQ=255)
                                      ccTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACACGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGCACCGGAACCTGAGCCGATCCCCg                                                                                                              >  2:779288/1‑150 (MQ=255)
                                      ccTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggcggct                                                                                                                                          <  1:1182342/122‑6 (MQ=255)
                                      ccTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggcggct                                                                                                                                          >  2:1182342/1‑117 (MQ=255)
                                      ccTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCg                                                                                                              >  2:808120/1‑150 (MQ=255)
                                         cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGACCCGGAACCTGAGCCGATCCCCGAAc                                                                                                           <  1:481601/150‑1 (MQ=255)
                                                  aGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGCGCCTGAGCCGATCCCCGCACCGCCAAaag                                                                                                  >  1:1169403/1‑150 (MQ=255)
                                                  aGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaag                                                                                                  >  1:1433409/1‑150 (MQ=255)
                                                  aGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAAACGCCACAAGCCGTTCAGCCGCCACAGGAGCCGGTGGTAGAGCCAGAACCTGAACCTGAGCCGATCCCCGCACCGCCAAaag                                                                                                  >  1:1219268/1‑150 (MQ=255)
                                                     ttATTGAACTACCTGCGACTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaagaag                                                                                               <  2:484551/150‑1 (MQ=255)
                                                      tATTGAACTACCTGCGCCTGCGCAGCCGAGTTCTGTCACGATGGTGACGCCTGCTGATCTCGCACCGCCCCACGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGCGCCAGCACCGGCACCTGAGCCGCTCCCCGCACCGCCCATAGAAGc                                                                                              >  1:136545/1‑150 (MQ=255)
                                                         tGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACACGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCAcc                                                                                           >  1:453673/1‑150 (MQ=255)
                                                               aCCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCTCGAACCGCCAAAAGAAGCACCggtggt                                                                                     >  2:1198738/1‑150 (MQ=255)
                                                                cctgcgcctgcgCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTc                                                                                    >  1:1356582/1‑150 (MQ=255)
                                                                 ctgcgcctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCa                                                                                   <  1:530136/150‑1 (MQ=255)
                                                                    cgcctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGCACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGCACCGCCAAAAGCAGCACCGGTGGTCATTg                                                                                >  2:1164937/1‑150 (MQ=255)
                                                                     ccctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGa                                                                               <  1:769271/149‑1 (MQ=255)
                                                                      cctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGaa                                                                              >  1:1536929/1‑150 (MQ=255)
                                                                         gcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaag                                                                           >  2:1187486/1‑150 (MQ=255)
                                                                           gcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTTGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagcc                                                                         <  1:438147/150‑1 (MQ=255)
                                                                           gcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagcc                                                                         >  2:1438519/1‑150 (MQ=255)
                                                                           gcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagcc                                                                         <  2:1433409/150‑1 (MQ=255)
                                                                            cAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagccg                                                                        >  2:1528135/1‑150 (MQ=255)
                                                                              gCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagccgaa                                                                      >  1:674969/1‑150 (MQ=255)
                                                                               ccGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagccgaag                                                                     <  1:1427540/150‑1 (MQ=255)
                                                                                cGATTTCTGTCACGATGGTTACGCCTGCTGAACTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagccgaagc                                                                    <  2:945292/150‑1 (MQ=255)
                                                                                cGATTTCTGGCACGAGGGTTACGCCTGCTTATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagccgaagc                                                                    <  1:1582569/150‑1 (MQ=255)
                                                                                      cTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAAc                                                              >  1:1092598/1‑150 (MQ=255)
                                                                                         tCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGACGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTa                                                           <  1:553941/150‑1 (MQ=255)
                                                                                           ccGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTATAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTatg                                                         <  2:1639252/149‑3 (MQ=255)
                                                                                            cGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGc                                                        <  1:122269/150‑1 (MQ=255)
                                                                                                ggTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCaaa                                                    <  1:1187486/150‑1 (MQ=255)
                                                                                                   tACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAAcc                                                 <  1:1262762/150‑1 (MQ=255)
                                                                                                    aCGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCg                                                >  1:739114/1‑150 (MQ=255)
                                                                                                     cGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGCACCGGACCCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCAGTGAAAAGCCGAAGCCGAAGCCGCAGCCAAACCCGa                                               >  2:94706/1‑150 (MQ=255)
                                                                                                         tgctgATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAGCCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTACGCCAAAACCGTAGcc                                           >  2:93670/1‑150 (MQ=255)
                                                                                                         tgctgATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGcc                                           <  2:1092598/150‑1 (MQ=255)
                                                                                                          gctgATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCg                                          >  2:1516314/1‑150 (MQ=255)
                                                                                                             gATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTg                                       <  2:674969/150‑1 (MQ=255)
                                                                                                              aTCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGa                                      <  1:1470037/150‑1 (MQ=255)
                                                                                                                  cGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGaaaaa                                  <  2:1698852/150‑1 (MQ=255)
                                                                                                                  cGAACCGCCACAAGCCGTGCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGACCCTGAGCCGAGCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGaaaaa                                  <  2:1019299/150‑1 (MQ=255)
                                                                                                                   gAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAACCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAg                                 >  1:363775/1‑150 (MQ=255)
                                                                                                                     aCCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGt                               <  1:418475/150‑1 (MQ=255)
                                                                                                                        gCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACa                            <  2:273210/150‑1 (MQ=255)
                                                                                                                         ccACAAGCCGTACAGCCGCCACCGGAGCCGGTGGTAGCGCCAGAACCCGAACCTGAGCCGATCCCCGACCCGCCAAAAGAAGCCCCGGTGGTCATTGACAAGCCGCAGCCGCAACCTCAGCCACAACCGCAGCCGGTGAAACAGGTACAg                           >  1:133275/1‑150 (MQ=255)
                                                                                                                           acaAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGaaaaa                                  >  1:1504017/1‑141 (MQ=255)
                                                                                                                           acaAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGaaaaa                                  <  2:1504017/141‑1 (MQ=255)
                                                                                                                                ccGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGagcagc                    <  1:525426/150‑1 (MQ=255)
                                                                                                                                 cGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGcc                   >  2:1681590/1‑150 (MQ=255)
                                                                                                                                 cGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGcc                   >  1:1387120/1‑150 (MQ=255)
                                                                                                                                     gctctaccACCGGCTCCGGTGGTAGAGCCAGAACCGGCACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCAGTGAAAAGCCGAAGCCGAAGCCTAAGCCAAAACCGAAGCCGGAGAAAAAGGTACAGGAGCAGCCataa               >  1:36912/7‑150 (MQ=255)
                                                                                                                                      aGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGCACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAAc              >  1:1009064/1‑150 (MQ=255)
                                                                                                                                      aGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAAc              >  2:739018/1‑150 (MQ=255)
                                                                                                                                       gccgccACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGCAGCCGGAGAAAAAGGTACAGGAGCAGCCAAACcg             >  2:1302973/1‑150 (MQ=255)
                                                                                                                                         cgccACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAAcgcg           <  2:1536929/150‑1 (MQ=255)
                                                                                                                                           ccACCGGAGCCGGTGGTAGAGCCAGAACCGGCACCTTAGCCGATCCCCGAAACGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCAGAATCCGAAACCTAAGCCAAAACCGACGCCGGTGAATAAGGTACAGGAGCCGCCAAACCGCGag         >  2:220190/1‑149 (MQ=255)
                                                                                                                                           caaCCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGAt         <  1:753226/148‑1 (MQ=255)
                                                                                                                                            cACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATg        <  2:1568290/150‑1 (MQ=255)
                                                                                                                                              ccGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTc      <  2:453673/150‑1 (MQ=255)
                                                                                                                                                  aGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAAc  <  1:1528135/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAAC  >  NC_000913/1311139‑1311434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: