Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,314,684 A→T 12.5% intergenic (+26/+34) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,314,6840AT12.5% 202.9 / 17.7 80intergenic (+26/+34)ompW/yciEouter membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (32/38);  new base T (7/3);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

TCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTT  >  NC_000913/1314539‑1314833
                                                                                                                                                 |                                                                                                                                                     
tCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACg                                                                                                                                                   <  2:1091468/150‑1 (MQ=255)
gcAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACg                                                                                                                                                   <  2:1097482/149‑1 (MQ=255)
  aGTGTGGTACATGGATATCGATACCACCGCTAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACggg                                                                                                                                                 <  1:908173/150‑1 (MQ=255)
     gtgGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTc                                                                                                                                              <  2:890445/150‑1 (MQ=255)
      tgGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcg                                                                                                                                             <  1:382487/150‑2 (MQ=255)
           cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAg                                                                                                                                        >  2:612089/1‑150 (MQ=255)
              gtttatCGATCCCACCGCAAATTATAAGCTGGGCGTTGCACATCAACACGATAGCGAACGCGTCGATCCGTGGGTGTTTATGTTCTCTGCAGGATATCGTTTTTAATCCCGAAAAAAAACGACCCCGAAATAAACGGGGTAAATAAGGAc                                                                                                                                     <  2:558338/147‑1 (MQ=255)
              ggATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGAc                                                                                                                                     <  2:1443719/150‑1 (MQ=255)
              ggATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGACAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGAc                                                                                                                                     <  1:578590/150‑1 (MQ=255)
               gATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGGCAATAAGGACa                                                                                                                                    >  2:1397054/1‑150 (MQ=255)
                atatCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACAt                                                                                                                                   >  2:1450591/1‑150 (MQ=255)
                  atCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCTTACGCCTCGATCCGTGTGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                                                                                 <  1:1198017/150‑1 (MQ=255)
                    cGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                                                                                                               <  1:601501/150‑1 (MQ=255)
                    cGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGGGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                                                                                                               <  1:607614/150‑1 (MQ=255)
                     gATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtat                                                                                                                              <  2:412814/150‑1 (MQ=255)
                         ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                                                                          >  2:624916/1‑150 (MQ=255)
                         ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                                                                          <  1:108882/150‑1 (MQ=255)
                          caccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTACTTACGCACAAAAACGACCCCGTAATATAGGGGGTCATTAAGGTCATGGTTTAAAgg                                                                                                                         >  1:820580/1‑150 (MQ=255)
                              gCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                                                                     >  1:702082/1‑150 (MQ=255)
                              gCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                                                                     >  1:759383/1‑150 (MQ=255)
                              gCCAATTATAAGCTGGGCGGTGCACAGAAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                                                                     <  1:1022322/150‑1 (MQ=255)
                                  aTTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTAGGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTattatt                                                                                                                 <  2:884038/150‑1 (MQ=255)
                                        aGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCg                                                                                                           >  2:531585/1‑150 (MQ=255)
                                        aGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCg                                                                                                           <  2:1242863/150‑1 (MQ=255)
                                                ggTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTACTATTTCTTCGCTTCTACg                                                                                                   <  2:1363669/150‑1 (MQ=255)
                                                ggTGCACAGCAACACGATAGCATACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACg                                                                                                   <  2:773329/150‑1 (MQ=255)
                                                  tGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGcc                                                                                                 >  2:177766/1‑150 (MQ=255)
                                                   gCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCa                                                                                                >  1:1599271/1‑150 (MQ=255)
                                                    cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                                                                                               >  2:1303711/1‑150 (MQ=255)
                                                        gCAACACGATAGCGTACGCCTAGATCCGTGGGTGTTTATGTTCTCAGCAGGATTTCGTTTTTAATTCCGCACAAAATCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATTAAGGGTATTTTTTCTTCGCTTGTACGCCATCAGt                                                                                           >  2:1312551/1‑150 (MQ=255)
                                                           acacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTc                                                                                        >  2:644279/1‑150 (MQ=255)
                                                            cacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAATTTCa                                                                                       <  1:660401/150‑1 (MQ=255)
                                                              cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATTGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga                                                                                     >  1:1067479/1‑150 (MQ=255)
                                                               gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCagag                                                                                    >  1:493761/1‑150 (MQ=255)
                                                               gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCagag                                                                                    <  2:1293552/150‑1 (MQ=255)
                                                                aTAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGc                                                                                   <  2:87020/150‑1 (MQ=255)
                                                                 tAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCg                                                                                  <  1:792648/150‑1 (MQ=255)
                                                                    cGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAAt                                                                               >  1:1482465/1‑150 (MQ=255)
                                                                     gTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAAtt                                                                              <  2:1651096/150‑1 (MQ=255)
                                                                        cGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaa                                                                           >  2:1088443/1‑150 (MQ=255)
                                                                         gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                          <  1:166172/150‑1 (MQ=255)
                                                                              gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGTGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                                                                     <  1:1088443/150‑1 (MQ=255)
                                                                                 ccGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCa                                                                  <  2:493761/150‑1 (MQ=255)
                                                                                        tgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGttt                                                           >  1:1639814/1‑150 (MQ=255)
                                                                                         gtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTg                                                          >  2:1199748/1‑150 (MQ=255)
                                                                                          tttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                         <  1:531585/150‑1 (MQ=255)
                                                                                          tttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                         <  1:1303711/150‑1 (MQ=255)
                                                                                          tttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                         >  1:933624/1‑150 (MQ=255)
                                                                                             aTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGAAATATACGGGGTCAATAATGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGa                                                      <  2:1366478/150‑1 (MQ=255)
                                                                                              tGTTCTCAGCAGGATATCGTTTTTAATTCCTCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGGAGTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGaa                                                     <  1:353833/150‑1 (MQ=255)
                                                                                               gTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                                    >  1:870056/1‑150 (MQ=255)
                                                                                               gTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                                    >  1:278521/1‑150 (MQ=255)
                                                                                               gTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                                    <  2:1701662/150‑1 (MQ=255)
                                                                                                    cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCt                                               <  2:1247726/150‑1 (MQ=255)
                                                                                                     agcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTg                                              <  2:759383/150‑1 (MQ=255)
                                                                                                      gcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGa                                             <  1:947583/150‑1 (MQ=255)
                                                                                                         ggATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATc                                          >  1:1004710/1‑150 (MQ=255)
                                                                                                           atatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAg                                        >  1:66385/1‑150 (MQ=255)
                                                                                                                gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGt                                   >  1:469217/1‑150 (MQ=255)
                                                                                                                gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGt                                   >  2:1377061/1‑150 (MQ=255)
                                                                                                                       aTTCCGCACAAAAACGACCCCGTAATATACTGGGTCAATAAGGACATCGAAAAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAt                            <  1:969142/150‑1 (MQ=255)
                                                                                                                          ccGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGcc                              <  2:712940/145‑1 (MQ=255)
                                                                                                                          ccGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGcc                              >  1:712940/1‑145 (MQ=255)
                                                                                                                           cGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTgg                        >  1:1687110/1‑149 (MQ=255)
                                                                                                                                cttattgACCCCGTATATTACGGGGTCGATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                      <  2:917572/125‑1 (MQ=255)
                                                                                                                                cttattgACCCCGTATATTACGGGGTCGATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                      >  1:917572/7‑131 (MQ=255)
                                                                                                                                cttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtttt                    <  1:796478/143‑1 (MQ=255)
                                                                                                                                cttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtttt                    >  2:796478/7‑149 (MQ=255)
                                                                                                                                 aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                  >  1:1556579/1‑150 (MQ=255)
                                                                                                                                 aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                  <  2:702082/150‑1 (MQ=255)
                                                                                                                                    ttgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCa               >  2:1124156/3‑150 (MQ=255)
                                                                                                                                    ttgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCa               >  2:1129989/3‑150 (MQ=255)
                                                                                                                                     tgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAt              >  1:1223495/2‑150 (MQ=255)
                                                                                                                                     cGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAt              >  2:197103/1‑150 (MQ=255)
                                                                                                                                     cGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAt              <  1:644279/150‑1 (MQ=255)
                                                                                                                                      gACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAtt             >  1:1690265/1‑150 (MQ=255)
                                                                                                                                      gACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAtt             <  1:624916/150‑1 (MQ=255)
                                                                                                                                       aCCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAttt            >  1:675585/1‑150 (MQ=255)
                                                                                                                                        ccccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCATTTGTTTGAGGAATATTCTGAATAAGCCAGTAGGACATTTGCTTTTCATCATTTa           >  2:174953/1‑150 (MQ=255)
                                                                                                                                          ccGTAATATACGGGGTCAATAAGGACATGGTATAAATGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTGGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa         >  2:99420/1‑150 (MQ=255)
                                                                                                                                               atatACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGc    <  2:1639814/150‑1 (MQ=255)
                                                                                                                                                tataCGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCt   >  2:561955/1‑150 (MQ=255)
                                                                                                                                                 ataCGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCtt  >  2:1407617/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTT  >  NC_000913/1314539‑1314833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: