Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,411,845 A→T 5.4% M35K (ATG→AAG) ‡ ttcA ← tRNA s(2)C32 thioltransferase, iron sulfur cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,411,8450AT 5.4% 269.2 / 4.6 93M35K (ATG→AAG) ‡ttcAtRNA s(2)C32 thioltransferase, iron sulfur cluster protein
Reads supporting (aligned to +/- strand):  ref base A (41/46);  new base T (2/3);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GCCCGGTTGCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTGGGGGCAAACT  >  NC_000913/1411700‑1411993
                                                                                                                                                 |                                                                                                                                                    
gCCCGGTTGCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATAtt                                                                                                                                                  >  2:984888/1‑150 (MQ=255)
 cccGGTTGCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTg                                                                                                                                                 <  2:98863/150‑1 (MQ=255)
    ggTTGCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAg                                                                                                                                              >  1:1107031/1‑150 (MQ=255)
    ggTTGCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAg                                                                                                                                              >  1:1112772/1‑150 (MQ=255)
    ggTTGCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAg                                                                                                                                              <  2:1008989/150‑1 (MQ=255)
       tGCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCa                                                                                                                                           >  1:712504/1‑150 (MQ=255)
        gCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAg                                                                                                                                          <  2:871946/150‑1 (MQ=255)
        gCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAg                                                                                                                                          <  2:1259772/150‑1 (MQ=255)
        gCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAg                                                                                                                                          <  1:1674835/150‑1 (MQ=255)
        gCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATATGATTGAAgaagg                                                                                                                                          <  1:1062096/150‑5 (MQ=255)
        gCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATATGATTGAAgaagg                                                                                                                                          <  2:1497417/150‑5 (MQ=255)
         cTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCatat                                                                                                                                                   >  1:336975/1‑140 (MQ=255)
         cTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCatat                                                                                                                                                   <  2:336975/140‑1 (MQ=255)
         cTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGc                                                                                                                                         >  2:1537524/1‑150 (MQ=255)
         cTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGc                                                                                                                                         >  1:913546/1‑150 (MQ=255)
          ttttGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCa                                                                                                                                        >  2:1481547/1‑150 (MQ=255)
             tGATCGATCTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATg                                                                                                                                     <  2:1008728/150‑1 (MQ=255)
               aTCGAGGTTAACAGCAACAAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGTATCTCGAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTATTCAATCATATTGAAGTCAGCAATGGc                                                                                                                                   <  2:1617007/150‑1 (MQ=255)
                tCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCt                                                                                                                                  <  2:736322/150‑1 (MQ=255)
                 cGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGAGATCGCATTCTTCAATCATTTTGAAGTCAGCAATGGCtt                                                                                                                                 >  1:234849/1‑150 (MQ=255)
                    ggTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGc                                                                                                                              <  1:817625/150‑1 (MQ=255)
                     gTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCACGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGcc                                                                                                                             >  1:145985/1‑150 (MQ=255)
                         aCAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACg                                                                                                                         >  1:392286/1‑150 (MQ=255)
                           aGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGtt                                                                                                                       <  2:775755/150‑1 (MQ=255)
                            gCCACCAGCGAAAAATTGATTGTCTCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCTATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTa                                                                                                                      <  1:477449/150‑1 (MQ=255)
                              caccaGCGAAAAATTGATTGGCGCGCTTAGCTGCAAATTGCGCAGAATCTCCAGCATGTATAGCTGTCTTTACCCCCGGAGAGGCCAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTacga                                                                                                                   >  1:270558/1‑150 (MQ=255)
                                ccaGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTacgac                                                                                                                  >  2:836788/1‑150 (MQ=255)
                                 caGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTacgacg                                                                                                                 >  2:518663/1‑150 (MQ=255)
                                 caGCGAAAAATTGATTGGCGCGCGTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTacgacg                                                                                                                 <  2:1035404/150‑1 (MQ=255)
                                  aGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGc                                                                                                                <  1:1333914/150‑1 (MQ=255)
                                     gAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGa                                                                                                             >  1:394057/1‑150 (MQ=255)
                                          attgattgGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGttt                                                                                                        >  2:1194436/1‑150 (MQ=255)
                                                  gcgcgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAAtt                                                                                                <  2:712504/150‑1 (MQ=255)
                                                  gcgcgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAAtt                                                                                                <  2:1538457/150‑1 (MQ=255)
                                                    gcgcTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTg                                                                                              >  1:1090891/1‑150 (MQ=255)
                                                      ggtttGCTGCAAATTGCGCAGAATCTCCTGAATTGTTTAGCTTTCTTTTCCGCCGGAGAGGCATACCATGATGCGATCGCATTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGTCGTTTTTGTAATTTGtt                                                                                            <  1:1280542/148‑1 (MQ=255)
                                                            ctgcAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGtt                                                                                      >  1:19873/1‑150 (MQ=255)
                                                            ctgcAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACACCCGGAGAGGCAAACCAAGATGCGATCGCCTTCTTAAATCATATTGAAGTCAGCAATGGCTACGCACACGTAACGACGCAGAAGTTTTTGAAATTTGTTCAGGtt                                                                                      >  1:622694/1‑150 (MQ=255)
                                                            ctgcAAATTGCGCAGAATCTCCAGCATGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTg                                                                                     <  2:270558/150‑1 (MQ=255)
                                                             tgcAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTg                                                                                     >  1:1574158/1‑150 (MQ=255)
                                                              gcAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGt                                                                                    >  1:1240425/1‑150 (MQ=255)
                                                                  aTTGCGCAGAATCTCCAGCATCGTATAGCTGACTTTACCCCCGGAGAGGTAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCTATGGCTTCGCCCACGTTACGACGCAGCCGTTTTTGAAATTTGTTCAGGTTGTATTg                                                                                >  2:1407015/1‑150 (MQ=255)
                                                                    tGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGtt                                                                              <  1:434257/150‑1 (MQ=255)
                                                                       gtaGATTCTCCATCATGGTATAGCTGTCTTTACCCCCTGAGAGGCAAACCATGATGCGAACGACTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGttctt                                                                           <  2:1636831/148‑1 (MQ=255)
                                                                         aGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGttctttc                                                                         <  2:1574158/150‑1 (MQ=255)
                                                                          gAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGttctttct                                                                        <  1:984888/150‑1 (MQ=255)
                                                                            aTCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTctttcttt                                                                      >  1:1629184/1‑150 (MQ=255)
                                                                              ctcCAGCATGGTATAGCTGTCTTTACCCGCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCACACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGt                                                                    <  2:145985/150‑1 (MQ=255)
                                                                                ccAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTaa                                                                  <  2:1107031/150‑1 (MQ=255)
                                                                                ccAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTaa                                                                  <  2:1112772/150‑1 (MQ=255)
                                                                                  aGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTATTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAAtt                                                                >  1:763214/1‑150 (MQ=255)
                                                                                   gCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAAttt                                                               >  1:34022/1‑150 (MQ=255)
                                                                                    cATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATttg                                                              <  2:596263/150‑1 (MQ=255)
                                                                                     aTGGTATAGCTGTCTTTACCCACGGAGAGGCAATCCATGATGCGATCGCCTTCTTCAATCATTTTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATttgt                                                             <  1:481531/150‑1 (MQ=255)
                                                                                         tataGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGAt                                                         >  1:478222/1‑150 (MQ=255)
                                                                                          ataGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGAtt                                                        >  2:5809/1‑150 (MQ=255)
                                                                                            aGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGAtttt                                                      <  1:351725/150‑1 (MQ=255)
                                                                                            aGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGAtttt                                                      >  2:970669/1‑150 (MQ=255)
                                                                                             gCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCATGTGGTATTGTTCTTTCTTTGTAATTTGTTGAttttg                                                     >  1:305236/1‑149 (MQ=255)
                                                                                             gCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTc                                                     >  1:1495002/1‑150 (MQ=255)
                                                                                              cTGTCTTTACCCCCGGAGAGGCAATCCATGATGCGATCCCCTTCTTCTATCATATTGAAGTCAGCAATGGCTTCGCCCTCGTTACGACGCTGACGTTTTTGTTATTTGTGCAGGGTGTTTTGTTCTTTCTTTGTAATTTGATGATTTTCt                                                    <  2:840245/150‑1 (MQ=255)
                                                                                              cTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCt                                                    <  2:1240425/150‑1 (MQ=255)
                                                                                               tGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCtt                                                   <  1:414655/150‑1 (MQ=255)
                                                                                                gTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTg                                                  <  1:836788/150‑1 (MQ=255)
                                                                                                     tACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCatta                                             <  2:1090891/150‑1 (MQ=255)
                                                                                                         cccGGAGAGGCAAACCATGATGCTATCGCCTTCTTCAATCATATTTAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTc                                         <  1:308026/150‑1 (MQ=255)
                                                                                                         cccGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTc                                         <  2:788626/150‑1 (MQ=255)
                                                                                                         cccGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTc                                         <  1:988419/150‑1 (MQ=255)
                                                                                                           cGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTTTTTCAg                                       >  2:953202/1‑150 (MQ=255)
                                                                                                            ggAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGt                                      <  1:1537524/150‑1 (MQ=255)
                                                                                                             gagaGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGtt                                     >  1:1349257/1‑150 (MQ=255)
                                                                                                                 ggCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTctct                                 >  1:642342/1‑150 (MQ=255)
                                                                                                                 ggCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTctct                                 >  2:1402786/1‑150 (MQ=255)
                                                                                                                   cAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTgg                               <  1:1194436/150‑1 (MQ=255)
                                                                                                                   cAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTGCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTgg                               <  2:913546/150‑1 (MQ=255)
                                                                                                                   cAAACCATGATGCAATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTgg                               <  2:1624815/150‑1 (MQ=255)
                                                                                                                       ccATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTAca                           >  1:16420/1‑149 (MQ=255)
                                                                                                                       ccATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACt                           >  1:1614419/1‑150 (MQ=255)
                                                                                                                               ctctctccTTCTTCAATCCAATAGAAGTCAGCTATGGGTTCGCCCACGTTCCGACGCAGACGTTTTTGTAATTTGGTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTAGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATgggg                   <  2:1335286/144‑1 (MQ=255)
                                                                                                                               cGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATgggg                   <  1:338876/150‑1 (MQ=255)
                                                                                                                                gATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGc                  >  2:1098094/1‑150 (MQ=255)
                                                                                                                                     ccTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAAtt             >  2:1688329/1‑150 (MQ=255)
                                                                                                                                     ccTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAAtt             >  2:1673618/1‑150 (MQ=255)
                                                                                                                                     ccTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAAtt             >  1:423476/1‑150 (MQ=255)
                                                                                                                                      tgacttcAATATGATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTg            >  1:467759/4‑150 (MQ=255)
                                                                                                                                      cttcttCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTg            >  1:1334516/1‑150 (MQ=255)
                                                                                                                                       gacttcAATATGATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCa                                        <  2:1174772/119‑1 (MQ=255)
                                                                                                                                       gacttcAATATGATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCa                                        >  1:1174772/3‑121 (MQ=255)
                                                                                                                                         cttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTgggg         <  2:394057/150‑1 (MQ=255)
                                                                                                                                         cttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTgggg         <  1:1092086/150‑1 (MQ=255)
                                                                                                                                         cttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGAAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTgggg         >  2:1455017/1‑150 (MQ=255)
                                                                                                                                         attcAATCATATTTAAGTCAGCAATGGCTTCGCGCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTCTTGTTCTTTCTTTGTAATTTGTTGATTTTCTAGCTTTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTgggg         <  1:171845/149‑1 (MQ=255)
                                                                                                                                          ttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGGAGAGGTTTTTGTAATTTGTTCTGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTggggg        <  1:1416356/150‑1 (MQ=255)
                                                                                                                                            aaaTCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTGGGGGCa      <  2:392286/149‑1 (MQ=255)
                                                                                                                                                cATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTGGGGGCAAACt  <  2:642342/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCCCGGTTGCTTTTGATCGAGGTTAACAGCCACCAGCGAAAAATTGATTGGCGCGCTTTGCTGCAAATTGCGCAGAATCTCCAGCATGGTATAGCTGTCTTTACCCCCGGAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTGTTGATTTTCTTGCATTATTTCAGTTCTCTGGTACTAAATGGGGCAAATTGGGGGCAAACT  >  NC_000913/1411700‑1411993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: