Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,417,469 C→A 7.3% intergenic (‑83/+19) recE ← / ← racC Rac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease/Rac prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,417,4690CA 7.3% 244.2 / 8.2 90intergenic (‑83/+19)recE/racCRac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease/Rac prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (45/38);  new base A (2/5);  total (47/43)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

GACGACGTCAGGTTCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTG  >  NC_000913/1417321‑1417617
                                                                                                                                                    |                                                                                                                                                    
gacgacGTCAGGTTCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCa                                                                                                                                                     >  1:1356311/1‑150 (MQ=255)
 acgacgTCAGGTTCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCAt                                                                                                                                                    >  1:612047/1‑150 (MQ=255)
       tCAGGTTCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAg                                                                                                                                              >  2:1029733/1‑150 (MQ=255)
        cAGGTTCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAgg                                                                                                                                             >  1:1167411/1‑150 (MQ=255)
            ttCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAGATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGTATGGCGCAGGCTAt                                                                                                                                         <  2:425186/150‑1 (MQ=255)
            ttCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGTATGGCGCAGGCTAt                                                                                                                                         <  2:1520955/150‑1 (MQ=255)
            ttCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGTATGGCGCAGGCTAt                                                                                                                                         <  1:337557/150‑1 (MQ=255)
            ttCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGTATGGCGCAGGCTAt                                                                                                                                         <  1:337787/150‑1 (MQ=255)
             tCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTAtt                                                                                                                                        >  1:1440717/1‑150 (MQ=255)
              cACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGTATGGCGCAGGCTAt                                                                                                                                         <  2:598409/148‑1 (MQ=255)
              cACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGTATGGCGCAGGCTAt                                                                                                                                         >  1:598409/1‑148 (MQ=255)
              cACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTAttt                                                                                                                                       >  1:1100933/1‑150 (MQ=255)
                 cGGATGATTTTTTCGCTTTCCGTAACAGGATGAGTGGTTTTGTGCTCAGTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGAAGGCAAtttctt                                                                                                                                    >  1:1322127/1‑150 (MQ=255)
                   gatgatTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCTCCATACGCAGGCTAtttctttc                                                                                                                                  <  2:1610293/150‑1 (MQ=255)
                   gatgatTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTAtttctttc                                                                                                                                  >  1:1122476/1‑150 (MQ=255)
                     tgatTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAg                                                                                                                                >  1:433292/1‑150 (MQ=255)
                          ttttCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTc                                                                                                                           <  1:466559/150‑1 (MQ=255)
                           tttCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCa                                                                                                                          >  1:395142/1‑150 (MQ=255)
                             tCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCAcc                                                                                                                        <  2:1679344/150‑1 (MQ=255)
                                   tCCGTAACAGTAATAGTGGTTTTGGGCTCATTGTTTTTTAACCTCAACTCAGAGTAAACTTCGTTTTGTTCATTGAATGAGCTTGCCGGATACACACTGTTCATAGCATGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTaa                                                                                                                  <  1:830815/150‑1 (MQ=255)
                                     cGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAAtt                                                                                                                <  1:1396353/150‑1 (MQ=255)
                                     cGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCACCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAAtt                                                                                                                >  1:648457/1‑150 (MQ=255)
                                       tAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTc                                                                                                              >  1:622672/1‑150 (MQ=255)
                                       tAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACAATGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCATATTTCACCTTTTTAttta                                                                                                              >  1:1623574/1‑149 (MQ=255)
                                          cAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCAtt                                                                                                           <  1:1064426/150‑1 (MQ=255)
                                           aGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTg                                                                                                          >  2:1536915/1‑150 (MQ=255)
                                              aaGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCaa                                                                                                       <  2:441411/150‑1 (MQ=255)
                                                 agTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTa                                                                                                    <  2:1100933/150‑1 (MQ=255)
                                                  gTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTag                                                                                                   >  1:461082/1‑150 (MQ=255)
                                                        ttGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGc                                                                                             <  2:1122476/150‑1 (MQ=255)
                                                         tgtgCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGcc                                                                                            >  1:1657073/1‑150 (MQ=255)
                                                         tgtgCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCCATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGcc                                                                                            <  1:1092704/150‑1 (MQ=255)
                                                         tgtgCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATAAGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGcc                                                                                            <  2:932364/150‑1 (MQ=255)
                                                          gtgCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCTGTGAATGATCTTGCCGGATACACACTGTTCATTGCCTGCGCCATACGCAGGCGTGTTCGTTCAGATTTCCCCTTGTATGTTCTATGCAAGTAGAGTTGcgt                                                                                           >  2:1617059/1‑148 (MQ=255)
                                                           tgCTCATTTTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAg                                                                                          <  2:1656192/150‑1 (MQ=255)
                                                            gCTCATTGTTTTTTAACCTCAACTCAGATTAAAAATCGTTTTGTTCAGAGAATGATCTTGCAGGATACACACTGTTCATAGCCTGCGCCAAACGCAGGCTATAACTATCAGATATCACAATTTAATTTCATTGAAATTAGAGATGACAGa                                                                                         >  1:1408042/1‑150 (MQ=255)
                                                              tCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGaaa                                                                                       >  2:1450844/1‑150 (MQ=255)
                                                                   gTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCgg                                                                                  <  2:1389250/150‑1 (MQ=255)
                                                                     tttttAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCt                                                                                <  1:307514/150‑1 (MQ=255)
                                                                      ttttAACCTCAACTCAGATTAAAATTCGTTTAGTTCAGTGAATGATCTTGCCGCATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTAAGATTTCTCCTTTTAATTTCATTGCAATTAGAGATGCCAGAAATTAGGCtt                                                                               >  2:115670/1‑150 (MQ=255)
                                                                         tAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCttttt                                                                            >  1:644247/1‑150 (MQ=255)
                                                                           aCCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCg                                                                                   >  1:1669668/1‑141 (MQ=255)
                                                                           aCCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCg                                                                                   <  2:1669668/141‑1 (MQ=255)
                                                                            ccTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCtttttttt                                                                         <  2:879288/150‑1 (MQ=255)
                                                                             cTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTc                                                                        >  2:447663/1‑150 (MQ=255)
                                                                              tCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCt                                                                       <  1:378292/150‑1 (MQ=255)
                                                                                aaCTCTTATTAAAATTCGTTTTGTTCAGTGAATTATCTTGCCGGATACACACTATTAATAGCCTGCGCCATACGCAGGATATTTCTTTCTGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGATATTCGGCTTTTTTTTCTGc                                                                     <  2:6476/150‑1 (MQ=255)
                                                                                 aCTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCg                                                                    <  2:251356/150‑1 (MQ=255)
                                                                                 aCTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCg                                                                    <  2:1600981/150‑1 (MQ=255)
                                                                                 aCTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCg                                                                    <  1:1450844/150‑1 (MQ=255)
                                                                                 aCTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCg                                                                    >  2:286794/1‑150 (MQ=255)
                                                                                      gATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAg                                                               >  2:1620627/1‑150 (MQ=255)
                                                                                       aTTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCTGGCAGa                                                              >  1:1190202/1‑150 (MQ=255)
                                                                                        ttAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGAt                                                             >  2:557728/1‑150 (MQ=255)
                                                                                               tCGTTTTGTACAGTTATTGATCTTGCCGGATTCACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGTTTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGTCTTTTTTTTCTGCGGGCAGATTCTTTcc                                                      <  1:925944/150‑1 (MQ=255)
                                                                                                 gTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGa                                                    >  1:1034418/1‑150 (MQ=255)
                                                                                                 gTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGa                                                    <  2:1440717/150‑1 (MQ=255)
                                                                                                 gTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGa                                                    >  2:1080515/1‑150 (MQ=255)
                                                                                                   tttGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGata                                                  <  2:622672/150‑1 (MQ=255)
                                                                                                     tGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATg                                                >  1:247310/1‑150 (MQ=255)
                                                                                                     tGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATg                                                >  2:977347/1‑150 (MQ=255)
                                                                                                     tGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATg                                                <  1:1139520/150‑1 (MQ=255)
                                                                                                     tGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATg                                                >  2:1634299/1‑150 (MQ=255)
                                                                                                     tGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATAAGCAGGCTAATTCTTTAAGATTTCACCTTTTAATTTCATTCCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTATGAGTGCAGATTCTTTCCGaaatc                                                >  2:1287526/1‑149 (MQ=255)
                                                                                                       ttgaGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCa                                              <  2:1578151/147‑1 (MQ=255)
                                                                                                       ttCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGAAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCa                                              <  1:70930/150‑1 (MQ=255)
                                                                                                           gTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAg                                          >  1:477880/1‑150 (MQ=255)
                                                                                                             gAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGc                                        >  2:377537/1‑150 (MQ=255)
                                                                                                               aTGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGcac                                      >  2:662809/1‑150 (MQ=255)
                                                                                                                    cTTGCCGGATACACACTGTTCATACCCTGCGCCATACGCAGGCTAATTCTTTCAGATTACACCTAATAATTTCATAGCAATTAGAGTTGCCAGAAATTCCCCTTTTATATCTGCGGGCAGATTCTTTCCGAAATGCACCAGGCACAattt                                 >  2:897116/1‑150 (MQ=255)
                                                                                                                      tGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAtttttt                               <  2:1167411/150‑1 (MQ=255)
                                                                                                                       gCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttttttt                              <  1:1029733/150‑1 (MQ=255)
                                                                                                                       gCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttttttt                              >  1:1489583/1‑150 (MQ=255)
                                                                                                                        ccGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTg                             <  1:1411267/150‑1 (MQ=255)
                                                                                                                        ccGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTg                             <  2:433292/150‑1 (MQ=255)
                                                                                                                         cGGATACACACTGTTCATAGCCTGCTCCATACGAAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGa                            <  2:1408042/150‑1 (MQ=255)
                                                                                                                          ggATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGac                           >  1:513841/1‑150 (MQ=255)
                                                                                                                           gATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGaca                          <  1:1634299/150‑1 (MQ=255)
                                                                                                                               cacacTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCtt                      >  1:763696/1‑150 (MQ=255)
                                                                                                                               cacacTGTTCATAGCCTGCACCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTATAGTTGCCAGAAATTCTGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCtt                      <  2:648457/150‑1 (MQ=255)
                                                                                                                                acacTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTtc                     >  1:1085082/1‑150 (MQ=255)
                                                                                                                                  acTGTTCATAGCCTGCGCCATTCGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTtcat                   >  2:1455883/1‑150 (MQ=255)
                                                                                                                                   cTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTtcatc                  <  1:666783/150‑1 (MQ=255)
                                                                                                                                    ttttCATATCCTGAGCCATACGCAGGCTATTTCTTTCATATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGACAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTtcatca                 <  2:1623574/148‑1 (MQ=255)
                                                                                                                                       tCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATAGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAATTTTTTGACACCTTAATCAAgt              >  2:1585236/1‑150 (MQ=255)
                                                                                                                                         aTAGCCTGCGCAATACGCAGGGTATTTCTTTCAGATTTCACCTTGTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCTGATTCATTCCGATATGCACCCGGCACTTTTTTTTGACTCCTTCTTGAAgtgt            >  1:265416/1‑150 (MQ=255)
                                                                                                                                          tAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGtt           >  1:650667/1‑150 (MQ=255)
                                                                                                                                            gCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGtttt         >  2:744226/1‑150 (MQ=255)
                                                                                                                                             ccTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGttttt        <  2:1657073/150‑1 (MQ=255)
                                                                                                                                              cTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTa       <  1:1620627/150‑1 (MQ=255)
                                                                                                                                                   ccATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTg  >  1:1679591/1‑150 (MQ=255)
                                                                                                                                                   ccATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTg  >  2:591023/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GACGACGTCAGGTTCACCGGATGATTTTTTCGCTTTCCGTAACAGGAAGAGTGGTTTTGTGCTCATTGTTTTTTAACCTCAACTCAGATTAAAATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTG  >  NC_000913/1417321‑1417617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: