Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,457,528 G→A 6.9% R11Q (CGA→CAA)  paaF → 2,3‑dehydroadipyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,457,5280GA 6.9% 315.6 / 6.2 100R11Q (CGA→CAA) paaF2,3‑dehydroadipyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base G (47/46);  new base A (2/5);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

Only 100 of 105 total aligned reads displayed.

CGATGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTGCCG  >  NC_000913/1457379‑1457677
                                                                                                                                                     |                                                                                                                                                     
cGATGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACg                                                                                                                                                       >  2:1258121/1‑150 (MQ=255)
cGATGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACg                                                                                                                                                       >  1:64544/1‑150 (MQ=255)
cGATGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGAAACg                                                                                                                                                       >  1:330192/1‑150 (MQ=255)
cGATGGAAACCAATTACAGTCTGGAACCAGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACg                                                                                                                                                       >  1:1592297/1‑150 (MQ=255)
        aCCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgt                                                                                                                                               <  1:1013940/150‑1 (MQ=255)
        aCCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgt                                                                                                                                               >  2:1115945/1‑150 (MQ=255)
         ccAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgtt                                                                                                                                              >  2:1655179/1‑150 (MQ=255)
         ccAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgtt                                                                                                                                              >  2:447335/1‑150 (MQ=255)
         ccAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgtt                                                                                                                                              >  2:749164/1‑150 (MQ=255)
          cAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTAttgttg                                                                                                                                             >  1:809839/1‑150 (MQ=255)
               aCAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACAATACTCGTTGCTGAc                                                                                                                                        <  2:11603/150‑1 (MQ=255)
               aCAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACAATACTCGTTGCTGAc                                                                                                                                        <  1:510599/150‑1 (MQ=255)
               aCAGTCTGGAACCGGAGGAACTCGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACAATACTCGTTGCTGAc                                                                                                                                        <  2:643974/150‑1 (MQ=255)
                cAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGAcc                                                                                                                                       <  2:620229/150‑1 (MQ=255)
                cAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGAcc                                                                                                                                       <  1:1704235/150‑1 (MQ=255)
                cAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACAATACTCGTTGCTGAcg                                                                                                                                       <  1:27552/150‑2 (MQ=255)
                  gTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGATTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCt                                                                                                                                     >  2:853971/1‑150 (MQ=255)
                  gTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCt                                                                                                                                     >  2:824012/1‑150 (MQ=255)
                   tCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCtt                                                                                                                                    >  1:1450706/1‑150 (MQ=255)
                     tGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTaa                                                                                                                                  >  2:441231/1‑150 (MQ=255)
                      ggAACCGGATGAACTGGCCGCAGGTTATGCGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGACAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAAc                                                                                                                                 >  2:1028893/1‑150 (MQ=255)
                       gAACCGGCTGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAAcc                                                                                                                                <  2:724388/150‑1 (MQ=255)
                       gAACCGGATGACCTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGATGACCAGCGATGTGGTGGTTGACTTTGCCGCGAAGGGGCTGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAAcc                                                                                                                                <  2:132212/150‑1 (MQ=255)
                        aaCCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCg                                                                                                                               >  1:1283533/1‑150 (MQ=255)
                        aaCCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAACAACGAGTATTGTTGCTGACCCTTAACCg                                                                                                                               <  2:1564036/150‑1 (MQ=255)
                        aaCCGGATGAACTGGCCGCAGCTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCg                                                                                                                               <  2:535472/150‑1 (MQ=255)
                               tGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTCGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGCTCGTCAGCCGTCAGCAACGAGTATTCTTGCTGACCCTTAACCGTCccgcc                                                                                                                        >  2:1718181/1‑150 (MQ=255)
                                  aCTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCa                                                                                                                     >  1:1692255/1‑150 (MQ=255)
                                  aCTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCa                                                                                                                     <  1:554556/150‑1 (MQ=255)
                                   cTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCAc                                                                                                                    >  1:1015027/1‑150 (MQ=255)
                                     ggCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGt                                                                                                                  <  2:851457/150‑1 (MQ=255)
                                          cAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATgc                                                                                                             >  1:1436014/1‑150 (MQ=255)
                                          cAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATgc                                                                                                             >  2:889101/1‑150 (MQ=255)
                                           aGGTTATGTGTTGAGGTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATgcg                                                                                                            <  1:187780/150‑1 (MQ=255)
                                             gTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCt                                                                                                          <  2:601320/150‑1 (MQ=255)
                                              ttATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTa                                                                                                         >  1:1559698/1‑150 (MQ=255)
                                               tATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTaa                                                                                                        <  2:256164/150‑1 (MQ=255)
                                                  gtgtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACACGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAaata                                                                                                     >  1:956772/1‑150 (MQ=255)
                                                   tgtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAaataa                                                                                                    <  2:840879/150‑1 (MQ=255)
                                                    gtTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAaataat                                                                                                   <  2:573527/150‑1 (MQ=255)
                                                      tGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGc                                                                                                 >  2:1109008/1‑150 (MQ=255)
                                                       gAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGcc                                                                                                <  1:493533/150‑1 (MQ=255)
                                                       gAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGcc                                                                                                >  2:114716/1‑150 (MQ=255)
                                                         gTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCct                                                                                              >  2:1442588/1‑150 (MQ=255)
                                                           tGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCATTAACCGTCCCGCCGCACGTAATGAGCTAAATAATGCCctgc                                                                                            >  2:1186133/1‑150 (MQ=255)
                                                           tGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACAAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCctgc                                                                                            >  2:1290938/1‑150 (MQ=255)
                                                            gCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCctgct                                                                                           >  1:840365/1‑150 (MQ=255)
                                                            gCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCctgct                                                                                           <  1:1695204/150‑1 (MQ=255)
                                                             ccAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGCGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACTTAATGCGCTAAATAATGCCctgctg                                                                                          >  2:691280/1‑150 (MQ=255)
                                                             ccAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGAGCTAAATAATTCCctgctt                                                                                          >  2:685140/1‑149 (MQ=255)
                                                                ggCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATg                                                                                       >  2:487223/1‑150 (MQ=255)
                                                                ggCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCATAACCGTCCCGCCGCACGTAAAGCGCTAAATAATGCCCTGCAGATg                                                                                       >  2:1367766/1‑150 (MQ=255)
                                                                  cACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCa                                                                                     <  1:749164/150‑1 (MQ=255)
                                                                  cACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCa                                                                                     >  2:1267622/1‑150 (MQ=255)
                                                                    cTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAATGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAAc                                                                                   <  1:812457/150‑1 (MQ=255)
                                                                     tGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACt                                                                                  <  2:958395/150‑1 (MQ=255)
                                                                      gCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTg                                                                                 <  2:330192/150‑1 (MQ=255)
                                                                      gCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTg                                                                                 <  2:1592297/150‑1 (MQ=255)
                                                                      gCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTg                                                                                 <  1:15022/150‑1 (MQ=255)
                                                                      gCCGCTGACCAGCGATGTGGGGGTTGACTTTGACGCGAAGGGGATGGCATGATCGAACTGATCTTCAGCCGTCATCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTg                                                                                 <  1:1054129/150‑1 (MQ=255)
                                                                         gCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTa                                                                              <  2:685533/150‑1 (MQ=255)
                                                                              ccAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGa                                                                         <  2:994030/150‑1 (MQ=255)
                                                                              ccAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGa                                                                         >  1:1572704/1‑150 (MQ=255)
                                                                              ccAGCGATGTGGTGGTTGACTTTGACACGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGa                                                                         <  2:956772/150‑1 (MQ=255)
                                                                                aGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAAc                                                                       <  2:1436014/150‑1 (MQ=255)
                                                                                 gCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATTATGCCCTGCTGATGCAACTGGTAAATGAACt                                                                      <  1:1115945/150‑1 (MQ=255)
                                                                                 gCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGTTGATACAACTGGTAAATGAACt                                                                      <  1:1258121/150‑1 (MQ=255)
                                                                                  cGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTg                                                                     >  1:1440884/1‑150 (MQ=255)
                                                                                     tgtgGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCTGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGaa                                                                  <  1:1367766/150‑1 (MQ=255)
                                                                                     tgtgGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGaa                                                                  <  1:487223/150‑1 (MQ=255)
                                                                                             ttGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGc                                                          <  1:441231/150‑1 (MQ=255)
                                                                                               gACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTa                                                        >  2:1549769/1‑150 (MQ=255)
                                                                                                 cTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTAcc                                                      >  2:188941/1‑150 (MQ=255)
                                                                                                 cTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTAcc                                                      >  1:385942/1‑150 (MQ=255)
                                                                                                 cTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTAcc                                                      <  2:1559698/150‑1 (MQ=255)
                                                                                                   ttGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGa                                                    >  2:452874/1‑150 (MQ=255)
                                                                                                    tGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGAt                                                   >  1:1351584/1‑150 (MQ=255)
                                                                                                      aCGCGAAGGGGATGGCATGAGCGAACTGATCGACAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATAc                                                 <  1:1028893/150‑1 (MQ=255)
                                                                                                        gcgAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCa                                               >  1:1154128/1‑150 (MQ=255)
                                                                                                           aaGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCa                                            <  1:1267622/150‑1 (MQ=255)
                                                                                                               ggATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTc                                        >  1:1008287/1‑150 (MQ=255)
                                                                                                               ggATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTc                                        >  1:1405721/1‑150 (MQ=255)
                                                                                                                 atcgCATGACCGAACTGAGCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGACCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCgg                                      <  1:70567/147‑1 (MQ=255)
                                                                                                                 aTGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCgg                                      <  2:1405721/150‑1 (MQ=255)
                                                                                                                         aGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtgtgtg                              >  2:671232/1‑150 (MQ=255)
                                                                                                                         aGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtgtgtg                              >  1:1605058/1‑150 (MQ=255)
                                                                                                                         aGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCtgtgtg                              <  1:1655179/150‑1 (MQ=255)
                                                                                                                           cGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGAt                            >  1:1069209/1‑150 (MQ=255)
                                                                                                                               cTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTAcc                        <  2:1015027/150‑1 (MQ=255)
                                                                                                                                   gcgtcagccgtcagcAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTa                    <  2:1315113/149‑1 (MQ=255)
                                                                                                                                    cgtcagccgtcagcAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCTCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTaa                   <  2:822077/150‑1 (MQ=255)
                                                                                                                                        agccgtcagcAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCa               <  2:1008287/150‑1 (MQ=255)
                                                                                                                                            gtcagcAACAATACTCGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCt           >  1:622530/1‑150 (MQ=255)
                                                                                                                                             tcagcAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGAAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCtt          >  1:1425599/1‑150 (MQ=255)
                                                                                                                                              cagaAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCttt         >  2:607696/1‑150 (MQ=255)
                                                                                                                                                 cAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCtttttt      <  1:1109008/150‑1 (MQ=255)
                                                                                                                                                  aaCGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTg     <  2:840365/150‑1 (MQ=255)
                                                                                                                                                  aaCGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTg     >  2:1549282/1‑150 (MQ=255)
                                                                                                                                                    cGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTGcc   >  1:363279/1‑150 (MQ=255)
                                                                                                                                                     gAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTGCCg  >  2:1427379/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGATGGAAACCAATTACAGTCTGGAACCGGATGAACTGGCCGCAGGTTATGTGTTGAGTTGCCAGGCACTGCCGCTGACCAGCGATGTGGTGGTTGACTTTGACGCGAAGGGGATGGCATGAGCGAACTGATCGTCAGCCGTCAGCAACGAGTATTGTTGCTGACCCTTAACCGTCCCGCCGCACGTAATGCGCTAAATAATGCCCTGCTGATGCAACTGGTAAATGAACTGGAAGCTGCGGCTACCGATACCAGCATTTCGGTCTGTGTGATTACCGGTAATGCACGCTTTTTTGCCG  >  NC_000913/1457379‑1457677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: