Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,475,548 A→C 5.7% A135A (GCA→GCC ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,475,5480AC 5.7% 300.5 / 5.7 99A135A (GCA→GCCydbDPF10971 family putative periplasmic methylglyoxal resistance protein
Reads supporting (aligned to +/- strand):  ref base A (48/45);  new base C (2/4);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAA  >  NC_000913/1475399‑1475697
                                                                                                                                                     |                                                                                                                                                     
aaTCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCa                                                                                                                                                       <  2:1237531/150‑1 (MQ=255)
aaTCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCa                                                                                                                                                       >  2:504342/1‑150 (MQ=255)
   cAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGt                                                                                                                                                    >  1:2969/1‑150 (MQ=255)
   cAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGt                                                                                                                                                    >  2:813637/1‑150 (MQ=255)
   cAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGAGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGt                                                                                                                                                    >  1:185516/1‑150 (MQ=255)
     aaaaTTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTgg                                                                                                                                                  >  2:223213/1‑150 (MQ=255)
        attgattgTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACt                                                                                                                                               >  1:1132860/1‑150 (MQ=255)
         ttgattgTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTg                                                                                                                                              <  1:1153646/150‑1 (MQ=255)
            atagTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTa                                                                                                                                           >  2:927485/4‑150 (MQ=255)
             ttgTTCTTGGGAATTTGACAGTCAAAGGTAATTTTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTAt                                                                                                                                          <  2:1537274/150‑1 (MQ=255)
              tgTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTAtt                                                                                                                                         <  2:24957/150‑1 (MQ=255)
                  cTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGGTTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATc                                                                                                                                     >  2:728740/1‑150 (MQ=255)
                  cTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATc                                                                                                                                     >  1:1049843/1‑150 (MQ=255)
                   ttGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCg                                                                                                                                    >  1:374455/1‑150 (MQ=255)
                   ttGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATACCAGTCCACTTGCATTTATCg                                                                                                                                    <  2:1591994/150‑1 (MQ=255)
                    tGGGAATTTTACAGTCAAAGGTAATATTTCCAATTTATATCTTTCTAATCCATTGGTTTTTCTCGGTAACGTGACGGCAAAGAATAGAGTTGCGGATTCACCACTGTGAAGTACAGGTTCGATAAATGCCAGTGGAATTGTATTTATCgg                                                                                                                                   >  2:229597/1‑149 (MQ=255)
                    tGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGCCGGCAACGAATATAGTTGCGGATTCACAACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCgc                                                                                                                                   >  2:1247546/1‑149 (MQ=255)
                    tGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCAGGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGa                                                                                                                                   >  2:1503733/1‑150 (MQ=255)
                     tggAATTTGACAGTCAAAGGTAATATTTCCACTTTCTATCTTTCTAATACATGGGTTATTCTCGGTAACGTGACTGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATACCAGTCCACTTGCATTTATCGaa                                                                                                                                  <  1:1689360/149‑2 (MQ=255)
                     gggAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGAc                                                                                                                                  <  2:1292230/150‑1 (MQ=255)
                     gggAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGAc                                                                                                                                  >  1:387177/1‑150 (MQ=255)
                     gggAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGAc                                                                                                                                  >  1:534015/1‑150 (MQ=255)
                       gAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTc                                                                                                                                <  2:835655/150‑1 (MQ=255)
                         aTTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCat                                                                                                                              >  1:1189152/1‑150 (MQ=255)
                         aTTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATACCAGTCCACTTGCATTTATCGaaccgg                                                                                                                              <  1:190157/150‑6 (MQ=38)
                         aTTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATACCAGTCCACTTGCATTTATCGaaccgg                                                                                                                              <  1:636248/150‑6 (MQ=38)
                          tttGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCata                                                                                                                             >  1:1597961/1‑150 (MQ=255)
                            tGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATAtt                                                                                                                           >  1:1605969/1‑150 (MQ=255)
                              aCAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTAc                                                                                                                         <  1:1365791/150‑1 (MQ=255)
                              aCAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTAc                                                                                                                         >  2:360471/1‑150 (MQ=255)
                               cAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCata                                                                                                                             >  1:934680/1‑145 (MQ=255)
                               cAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCata                                                                                                                             <  2:934680/145‑1 (MQ=255)
                                aGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGa                                                                                                                       >  2:740738/1‑150 (MQ=255)
                                 gTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATTGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGAt                                                                                                                      <  2:570138/150‑1 (MQ=255)
                                 gTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGAt                                                                                                                      <  2:1597961/150‑1 (MQ=255)
                                  tCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATa                                                                                                                     <  2:1132860/150‑1 (MQ=255)
                                     aaGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACAATAATc                                                                                                                  <  2:70604/150‑1 (MQ=255)
                                         tAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGta                                                                                                              >  1:32781/1‑149 (MQ=255)
                                         tAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTc                                                                                                              >  1:147742/1‑150 (MQ=255)
                                            tatTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTAc                                                                                                           <  2:387177/150‑1 (MQ=255)
                                             atTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACg                                                                                                          <  2:1489167/150‑1 (MQ=255)
                                                   aCTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAg                                                                                                    >  1:185570/1‑150 (MQ=255)
                                                    cTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAgg                                                                                                   <  1:504342/150‑1 (MQ=255)
                                                      ttCTCTCTTTATAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAgggg                                                                                                 <  1:353687/150‑1 (MQ=255)
                                                           tctTTCTAATCCATAGGTTATTCTCGGTAACGTGACGGCAAAGAATATATTTGCGGATTCACCACTGTTAATTACCGGTTAGATAAATGCAAGTGGACTGGTATTTATCGCCTCATATTACGATAATCCGTCTACGATTTAGGGGAGTAt                                                                                            >  2:351097/1‑150 (MQ=255)
                                                            ctTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCTATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTAtt                                                                                           <  2:147742/150‑1 (MQ=255)
                                                             tttCTAATCAATTGGTTATTCTCGGTAACGTGACGGCAACGAATTTAGTTGAGGATTCACCACTGTTAATTACCGGTTGGATAAATGCAATTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTa                                                                                          <  1:306252/150‑1 (MQ=255)
                                                              ttCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTaa                                                                                         >  2:1296863/1‑150 (MQ=255)
                                                                cTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATg                                                                                       <  1:365113/150‑1 (MQ=255)
                                                                  aaTCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATgcg                                                                                     <  1:1247287/150‑1 (MQ=255)
                                                                  aaTCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATgcg                                                                                     >  1:1317048/1‑150 (MQ=255)
                                                                  aaTCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATgcg                                                                                     >  1:1366065/1‑150 (MQ=255)
                                                                    tCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTCCCGTTTCGATAACTGCAAGTTGACTGGTTTTTATCGACTCATTTTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGcgcg                                                                                   <  1:475591/150‑1 (MQ=255)
                                                                      cATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTg                                                                                 >  1:64879/1‑150 (MQ=255)
                                                                        tGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTggg                                                                               <  1:994870/150‑1 (MQ=255)
                                                                         gggTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATAATACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGa                                                                              <  2:245532/150‑1 (MQ=255)
                                                                          ggTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGat                                                                             >  2:1504743/1‑150 (MQ=255)
                                                                             tATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTTGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATAtt                                                                          >  1:346296/1‑150 (MQ=255)
                                                                               ttCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTa                                                                        >  1:622323/1‑150 (MQ=255)
                                                                                 ctcGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATc                                                                      >  2:503079/1‑150 (MQ=255)
                                                                                     gTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATg                                                                  <  2:1170770/150‑1 (MQ=255)
                                                                                     gTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATg                                                                  >  2:922793/1‑150 (MQ=255)
                                                                                      tAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGa                                                                 <  2:185516/150‑1 (MQ=255)
                                                                                       aaCTTGACGGCAACGATTATAGTTGCGGATTCACCACTGTTAAATCCCGGATCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGAc                                                                <  2:1136093/150‑1 (MQ=255)
                                                                                          gTGACGTCAACGAATATAGTTGCGGATTCACCACTGTTTATTACCGGTTCGATAAATGCATGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTCTTAATGCGCGTGGGATATTTATCAATGACATa                                                             <  2:817020/150‑1 (MQ=255)
                                                                                          gTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATa                                                             >  2:356444/1‑150 (MQ=255)
                                                                                              cGGCAACGAATATAGTTTCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTg                                                         <  1:1458492/150‑1 (MQ=255)
                                                                                              cGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTg                                                         >  2:381971/1‑150 (MQ=255)
                                                                                                gCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCa                                                       <  1:1692978/150‑1 (MQ=255)
                                                                                                   aCGAATATAGTTGCGGATTAAGCACTGTTAATTACCGGTTCGATAAATGCAAGTTGACTGGTAATTATCGACTCCTATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCt                                                    >  2:253927/1‑150 (MQ=255)
                                                                                                    cGAATATAGTTGCGGGTTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtg                                                   <  1:728740/150‑1 (MQ=255)
                                                                                                     gAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtgt                                                  <  2:374455/150‑1 (MQ=255)
                                                                                                      aaTATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtgtt                                                 <  1:1306930/150‑1 (MQ=255)
                                                                                                        tataGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtgttgt                                               <  2:682776/150‑1 (MQ=255)
                                                                                                        tataGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtgttgt                                               <  1:740738/150‑1 (MQ=255)
                                                                                                         ataGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTTCGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTgttgtt                                              <  2:908021/150‑1 (MQ=255)
                                                                                                         ataGGTGCGGATTCACCACTGTTTATTTCCTGTTCGATAAATGCAAGTGTACTGGTATTTATCGACTCATATTACGATTATCCGTCTACGATTAAGGGTAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTgttgtt                                              <  2:86423/150‑1 (MQ=255)
                                                                                                          taGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGttgttg                                             >  2:526801/1‑150 (MQ=255)
                                                                                                            gTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACATGTTGTTGCg                                           >  1:348733/1‑150 (MQ=255)
                                                                                                              tGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGTGATATTTATCAATGACATAATTTCACCTGTTGTTGCGTc                                         >  1:463209/1‑150 (MQ=255)
                                                                                                                 ggATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTc                                      <  2:534015/150‑1 (MQ=255)
                                                                                                                  gATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCg                                     <  2:365955/150‑1 (MQ=255)
                                                                                                                   aTTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGa                                    >  2:154719/1‑150 (MQ=255)
                                                                                                                     tCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACa                                  <  2:1317048/150‑1 (MQ=255)
                                                                                                                      caccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACaa                                 >  2:1340621/1‑150 (MQ=255)
                                                                                                                      caccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACaa                                 >  2:1301360/1‑150 (MQ=255)
                                                                                                                      caccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACaa                                 >  1:1361359/1‑150 (MQ=255)
                                                                                                                       accacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACaaa                                <  1:360471/150‑1 (MQ=255)
                                                                                                                        ccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAAt                               <  1:1314432/150‑1 (MQ=255)
                                                                                                                        ccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAAt                               <  1:154719/150‑1 (MQ=255)
                                                                                                                                 aTTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCTTATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTc                      >  1:1203940/1‑150 (MQ=255)
                                                                                                                                 aTTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAAtta                      >  2:495700/1‑149 (MQ=255)
                                                                                                                                     ccGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATgg                  <  1:159114/150‑1 (MQ=255)
                                                                                                                                        agtCGATAAATACCAGTCCACTTGCATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCTCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTcc               >  2:89496/3‑150 (MQ=255)
                                                                                                                                         ttCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCg              <  1:223213/150‑1 (MQ=255)
                                                                                                                                           cGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGAAATAATTGCACCTGTTGTTGAGTCTTCGACAAATAGTGAATTCATTGTCAGTg            >  2:1682811/1‑150 (MQ=255)
                                                                                                                                                aaTGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGt       >  1:681428/1‑150 (MQ=255)
                                                                                                                                                    cAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCg              >  2:1458423/1‑139 (MQ=255)
                                                                                                                                                    cAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCg              <  1:1458423/139‑1 (MQ=255)
                                                                                                                                                     aaGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGTGGAGTATTAATTCGCGTGGTATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTAATGGTCAGTGCTAGTGACaa  >  1:1447730/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAA  >  NC_000913/1475399‑1475697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: