Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,475,557 G→T 6.8% L138L (CTG→CTT ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,475,5570GT 6.8% 282.6 / 5.1 95L138L (CTG→CTTydbDPF10971 family putative periplasmic methylglyoxal resistance protein
Reads supporting (aligned to +/- strand):  ref base G (44/44);  new base T (3/4);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATG  >  NC_000913/1475408‑1475702
                                                                                                                                                     |                                                                                                                                                 
ttgattgTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTg                                                                                                                                                   <  1:1153646/150‑1 (MQ=255)
   atagTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTa                                                                                                                                                >  2:927485/4‑150 (MQ=255)
    ttgTTCTTGGGAATTTGACAGTCAAAGGTAATTTTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTAt                                                                                                                                               <  2:1537274/150‑1 (MQ=255)
     tgTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTAtt                                                                                                                                              <  2:24957/150‑1 (MQ=255)
         cTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGGTTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATc                                                                                                                                          >  2:728740/1‑150 (MQ=255)
         cTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATc                                                                                                                                          >  1:1049843/1‑150 (MQ=255)
          ttGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCg                                                                                                                                         >  1:374455/1‑150 (MQ=255)
          ttGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATACCAGTCCACTTGCATTTATCg                                                                                                                                         <  2:1591994/150‑1 (MQ=255)
           tGGGAATTTTACAGTCAAAGGTAATATTTCCAATTTATATCTTTCTAATCCATTGGTTTTTCTCGGTAACGTGACGGCAAAGAATAGAGTTGCGGATTCACCACTGTGAAGTACAGGTTCGATAAATGCCAGTGGAATTGTATTTATCgg                                                                                                                                        >  2:229597/1‑149 (MQ=255)
           tGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGCCGGCAACGAATATAGTTGCGGATTCACAACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCgc                                                                                                                                        >  2:1247546/1‑149 (MQ=255)
           tGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCAGGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGa                                                                                                                                        >  2:1503733/1‑150 (MQ=255)
            tggAATTTGACAGTCAAAGGTAATATTTCCACTTTCTATCTTTCTAATACATGGGTTATTCTCGGTAACGTGACTGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATACCAGTCCACTTGCATTTATCGaa                                                                                                                                       <  1:1689360/149‑2 (MQ=255)
            gggAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGAc                                                                                                                                       >  1:387177/1‑150 (MQ=255)
            gggAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGAc                                                                                                                                       >  1:534015/1‑150 (MQ=255)
            gggAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGAc                                                                                                                                       <  2:1292230/150‑1 (MQ=255)
              gAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTc                                                                                                                                     <  2:835655/150‑1 (MQ=255)
                aTTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCat                                                                                                                                   >  1:1189152/1‑150 (MQ=255)
                aTTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATACCAGTCCACTTGCATTTATCGaaccgg                                                                                                                                   <  1:636248/150‑6 (MQ=38)
                aTTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATACCAGTCCACTTGCATTTATCGaaccgg                                                                                                                                   <  1:190157/150‑6 (MQ=38)
                 tttGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCata                                                                                                                                  >  1:1597961/1‑150 (MQ=255)
                   tGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATAtt                                                                                                                                >  1:1605969/1‑150 (MQ=255)
                     aCAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTAc                                                                                                                              >  2:360471/1‑150 (MQ=255)
                     aCAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTAc                                                                                                                              <  1:1365791/150‑1 (MQ=255)
                      cAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCata                                                                                                                                  <  2:934680/145‑1 (MQ=255)
                      cAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCata                                                                                                                                  >  1:934680/1‑145 (MQ=255)
                       aGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGa                                                                                                                            >  2:740738/1‑150 (MQ=255)
                        gTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATTGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGAt                                                                                                                           <  2:570138/150‑1 (MQ=255)
                        gTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGAt                                                                                                                           <  2:1597961/150‑1 (MQ=255)
                         tCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATa                                                                                                                          <  2:1132860/150‑1 (MQ=255)
                           aaaGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAAt                                                                                                                        <  2:956224/150‑1 (MQ=255)
                            aaGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACAATAATc                                                                                                                       <  2:70604/150‑1 (MQ=255)
                                tAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGta                                                                                                                   >  1:32781/1‑149 (MQ=255)
                                tAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTc                                                                                                                   >  1:147742/1‑150 (MQ=255)
                                   tatTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTAc                                                                                                                <  2:387177/150‑1 (MQ=255)
                                    atTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACg                                                                                                               <  2:1489167/150‑1 (MQ=255)
                                          aCTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAg                                                                                                         >  1:185570/1‑150 (MQ=255)
                                           cTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAgg                                                                                                        <  1:504342/150‑1 (MQ=255)
                                             ttCTCTCTTTATAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAgggg                                                                                                      <  1:353687/150‑1 (MQ=255)
                                                  tctTTCTAATCCATAGGTTATTCTCGGTAACGTGACGGCAAAGAATATATTTGCGGATTCACCACTGTTAATTACCGGTTAGATAAATGCAAGTGGACTGGTATTTATCGCCTCATATTACGATAATCCGTCTACGATTTAGGGGAGTAt                                                                                                 >  2:351097/1‑150 (MQ=255)
                                                   ctTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCTATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTAtt                                                                                                <  2:147742/150‑1 (MQ=255)
                                                    tttCTAATCAATTGGTTATTCTCGGTAACGTGACGGCAACGAATTTAGTTGAGGATTCACCACTGTTAATTACCGGTTGGATAAATGCAATTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTa                                                                                               <  1:306252/150‑1 (MQ=255)
                                                     ttCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTaa                                                                                              >  2:1296863/1‑150 (MQ=255)
                                                       cTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATg                                                                                            <  1:365113/150‑1 (MQ=255)
                                                         aaTCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATgcg                                                                                          >  1:1366065/1‑150 (MQ=255)
                                                         aaTCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATgcg                                                                                          >  1:1317048/1‑150 (MQ=255)
                                                         aaTCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATgcg                                                                                          <  1:1247287/150‑1 (MQ=255)
                                                           tCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTCCCGTTTCGATAACTGCAAGTTGACTGGTTTTTATCGACTCATTTTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGcgcg                                                                                        <  1:475591/150‑1 (MQ=255)
                                                             cATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTg                                                                                      >  1:64879/1‑150 (MQ=255)
                                                               tGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTggg                                                                                    <  1:994870/150‑1 (MQ=255)
                                                                gggTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATAATACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGa                                                                                   <  2:245532/150‑1 (MQ=255)
                                                                 ggTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGat                                                                                  >  2:1504743/1‑150 (MQ=255)
                                                                    tATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTTGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATAtt                                                                               >  1:346296/1‑150 (MQ=255)
                                                                      ttCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTa                                                                             >  1:622323/1‑150 (MQ=255)
                                                                        ctcGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATc                                                                           >  2:503079/1‑150 (MQ=255)
                                                                            gTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATg                                                                       <  2:1170770/150‑1 (MQ=255)
                                                                            gTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATg                                                                       >  2:922793/1‑150 (MQ=255)
                                                                             tAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGa                                                                      <  2:185516/150‑1 (MQ=255)
                                                                              aaCTTGACGGCAACGATTATAGTTGCGGATTCACCACTGTTAAATCCCGGATCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGAc                                                                     <  2:1136093/150‑1 (MQ=255)
                                                                                 gTGACGTCAACGAATATAGTTGCGGATTCACCACTGTTTATTACCGGTTCGATAAATGCATGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTCTTAATGCGCGTGGGATATTTATCAATGACATa                                                                  <  2:817020/150‑1 (MQ=255)
                                                                                 gTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATa                                                                  >  2:356444/1‑150 (MQ=255)
                                                                                     cGGCAACGAATATAGTTTCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTg                                                              <  1:1458492/150‑1 (MQ=255)
                                                                                     cGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTg                                                              >  2:381971/1‑150 (MQ=255)
                                                                                       gCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCa                                                            <  1:1692978/150‑1 (MQ=255)
                                                                                          aCGAATATAGTTGCGGATTAAGCACTGTTAATTACCGGTTCGATAAATGCAAGTTGACTGGTAATTATCGACTCCTATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCt                                                         >  2:253927/1‑150 (MQ=255)
                                                                                           cGAATATAGTTGCGGGTTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtg                                                        <  1:728740/150‑1 (MQ=255)
                                                                                            gAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtgt                                                       <  2:374455/150‑1 (MQ=255)
                                                                                             aaTATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtgtt                                                      <  1:1306930/150‑1 (MQ=255)
                                                                                               tataGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtgttgt                                                    <  2:682776/150‑1 (MQ=255)
                                                                                               tataGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCtgttgt                                                    <  1:740738/150‑1 (MQ=255)
                                                                                                ataGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTTCGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTgttgtt                                                   <  2:908021/150‑1 (MQ=255)
                                                                                                ataGGTGCGGATTCACCACTGTTTATTTCCTGTTCGATAAATGCAAGTGTACTGGTATTTATCGACTCATATTACGATTATCCGTCTACGATTAAGGGTAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTgttgtt                                                   <  2:86423/150‑1 (MQ=255)
                                                                                                 taGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGttgttg                                                  >  2:526801/1‑150 (MQ=255)
                                                                                                   gTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACATGTTGTTGCg                                                >  1:348733/1‑150 (MQ=255)
                                                                                                     tGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGTGATATTTATCAATGACATAATTTCACCTGTTGTTGCGTc                                              >  1:463209/1‑150 (MQ=255)
                                                                                                        ggATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTc                                           >  2:1170261/1‑150 (MQ=255)
                                                                                                        ggATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTc                                           <  2:534015/150‑1 (MQ=255)
                                                                                                         gATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCg                                          <  2:365955/150‑1 (MQ=255)
                                                                                                          aTTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGa                                         >  2:154719/1‑150 (MQ=255)
                                                                                                            tCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACa                                       <  2:1317048/150‑1 (MQ=255)
                                                                                                             caccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACaa                                      >  2:1340621/1‑150 (MQ=255)
                                                                                                             caccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACaa                                      >  2:1301360/1‑150 (MQ=255)
                                                                                                             caccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACaa                                      >  1:1361359/1‑150 (MQ=255)
                                                                                                              accacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACaaa                                     <  1:360471/150‑1 (MQ=255)
                                                                                                               ccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAAt                                    <  1:1314432/150‑1 (MQ=255)
                                                                                                               ccacTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAAt                                    <  1:154719/150‑1 (MQ=255)
                                                                                                                        aTTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCTTATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTc                           >  1:1203940/1‑150 (MQ=255)
                                                                                                                        aTTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAAtta                           >  2:495700/1‑149 (MQ=255)
                                                                                                                            ccGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATgg                       <  1:159114/150‑1 (MQ=255)
                                                                                                                               agtCGATAAATACCAGTCCACTTGCATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCTCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTcc                    >  2:89496/3‑150 (MQ=255)
                                                                                                                                ttCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCg                   <  1:223213/150‑1 (MQ=255)
                                                                                                                                  cGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGAAATAATTGCACCTGTTGTTGAGTCTTCGACAAATAGTGAATTCATTGTCAGTg                 >  2:1682811/1‑150 (MQ=255)
                                                                                                                                       aaTGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGt            >  1:681428/1‑150 (MQ=255)
                                                                                                                                           cAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCg                   <  1:1458423/139‑1 (MQ=255)
                                                                                                                                           cAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCg                   >  2:1458423/1‑139 (MQ=255)
                                                                                                                                            aaGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGTGGAGTATTAATTCGCGTGGTATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTAATGGTCAGTGCTAGTGACaa       >  1:1447730/1‑150 (MQ=255)
                                                                                                                                             aGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACaaa      >  2:379872/1‑150 (MQ=255)
                                                                                                                                                     ggTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATg  >  1:1401130/1‑146 (MQ=255)
                                                                                                                                                     ggTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATg  <  2:1401130/146‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACCGGTTCGATAAATGCAAGTGGACTGGTATTTATCGACTCATATTACGATAATCCGTCTACGATTAAGGGGAGTATTAATGCGCGTGGGATATTTATCAATGACATAATTGCACCTGTTGTTGCGTCTTCGACAAATAGTGAATTCATGGTCCGTGCGAGTGACAAACATG  >  NC_000913/1475408‑1475702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: