Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,488,063 T→C 6.7% intergenic (+28/‑169) ydcF → / → aldA DUF218 superfamily protein, SAM‑binding/aldehyde dehydrogenase A, NAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,488,0630TC 6.7% 298.8 / 9.9 104intergenic (+28/‑169)ydcF/aldADUF218 superfamily protein, SAM‑binding/aldehyde dehydrogenase A, NAD‑linked
Reads supporting (aligned to +/- strand):  ref base T (53/44);  new base C (4/3);  total (57/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

Only 100 of 108 total aligned reads displayed.

TCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGAAAACAAACATA  >  NC_000913/1487915‑1488212
                                                                                                                                                    |                                                                                                                                                     
tCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTg                                                                                                                                                      <  1:1677863/150‑1 (MQ=255)
gccccGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTg                                                                                                                                                      <  2:1532079/149‑1 (MQ=255)
  cccGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTgaa                                                                                                                                                    >  2:871633/1‑148 (MQ=255)
  cccGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCa                                                                                                                                                    >  2:1420896/1‑150 (MQ=255)
  cccGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCa                                                                                                                                                    <  2:1702048/150‑1 (MQ=255)
      cgGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATTGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACg                                                                                                                                                <  2:414608/150‑1 (MQ=255)
      cgGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACg                                                                                                                                                >  2:1052288/1‑150 (MQ=255)
         gCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAATAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGc                                                                                                                                             >  2:182015/1‑150 (MQ=255)
         gCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGc                                                                                                                                             <  1:1705826/150‑1 (MQ=255)
         gCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGc                                                                                                                                             >  2:487608/1‑150 (MQ=255)
         gCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGc                                                                                                                                             >  2:822403/1‑150 (MQ=255)
          cGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCa                                                                                                                                            >  2:686686/1‑150 (MQ=255)
            agaTTTTATCGTTCACGTTGATATTCCGGCAGAAGTCATACATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTAGTAACGTAAAAAATTGCCAGTTTGTGAACCACTTGTTTGCAAAAGGGCAtt                                                                                                                                          >  1:935328/1‑149 (MQ=255)
              aTTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGAc                                                                                                                                        <  1:1282851/150‑1 (MQ=255)
                 ttATCGTTCACGTTGATTTTCCGGCAGACGTCACCCCTGCATCGCAACCGCTGAAACAGGATGCGGCGCTCCTCGAGGCGAGGGAATGTCGCGCGTTACGTTAAAAATAGCCCGTTTGTCACCCACTTGTTTGCAAACGGGCATGACTcc                                                                                                                                     <  2:613047/150‑1 (MQ=255)
                 ttATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTcc                                                                                                                                     <  1:686686/150‑1 (MQ=255)
                 taaTCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGGGCTCATCGAGGCGATGGAAAGACGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTcc                                                                                                                                     <  1:1666098/148‑1 (MQ=255)
                  tATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCt                                                                                                                                    >  2:1522287/1‑150 (MQ=255)
                   aTCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTg                                                                                                                                   <  1:634275/150‑1 (MQ=255)
                    tCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGa                                                                                                                                  <  2:778073/150‑1 (MQ=255)
                    tCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGa                                                                                                                                  <  2:41614/150‑1 (MQ=255)
                           cGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTAt                                                                                                                           >  2:1405396/1‑150 (MQ=255)
                            gTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTAtt                                                                                                                          <  2:1633353/150‑1 (MQ=255)
                            gTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTAtt                                                                                                                          <  2:506924/150‑1 (MQ=255)
                              tGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTc                                                                                                                        >  1:1011117/1‑150 (MQ=255)
                               gATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCt                                                                                                                       <  2:891817/150‑1 (MQ=255)
                                      cGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCtttt                                                                                                                >  2:1318075/1‑150 (MQ=255)
                                       ggCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTa                                                                                                               >  1:267343/1‑150 (MQ=255)
                                         cAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGCATCCTGACTTTTATTTCTGCCTTTTAtt                                                                                                             >  1:1645526/1‑150 (MQ=255)
                                          aGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTc                                                                                                            >  1:590230/1‑150 (MQ=255)
                                             aGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtt                                                                                                         >  1:329913/1‑150 (MQ=255)
                                             aGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtt                                                                                                         <  2:329913/150‑1 (MQ=255)
                                              gTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCttt                                                                                                        >  1:753748/1‑150 (MQ=255)
                                               tCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtttt                                                                                                       >  2:1321752/1‑150 (MQ=255)
                                               tCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtttt                                                                                                       <  1:980780/150‑1 (MQ=255)
                                                cATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTa                                                                                                      >  2:1545867/1‑150 (MQ=255)
                                                 aTCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTac                                                                                                     >  2:201275/1‑150 (MQ=255)
                                                 aTCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTACTTTTaa                                                                                                     >  1:687611/1‑149 (MQ=255)
                                                     atgcatgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACtt                                                                                                 <  1:822403/150‑1 (MQ=255)
                                                      tgcatgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTg                                                                                                >  1:1154034/1‑150 (MQ=255)
                                                      tgcatgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTg                                                                                                <  2:953729/150‑1 (MQ=255)
                                                          tgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGtttt                                                                                            >  2:90131/1‑150 (MQ=255)
                                                            gctaaCGATGAAACATGATGCTGTGCTCTTAGAGGCGATGGAAAGTCGTGCGTTACGTTAAAAATTGCCCGTTTGTGATCCACTTGTTTGCAATCGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTa                                                                                          <  2:1047844/147‑1 (MQ=255)
                                                            gCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTa                                                                                          >  1:758980/1‑150 (MQ=255)
                                                             ccaaCGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCCCTTGTATGCAAACGGGCATGACTCCTGACATTTATTTCTGCCTTTTATTCCTTTTACACTTCTTTTTAt                                                                                         >  1:262320/3‑150 (MQ=255)
                                                             cAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTAt                                                                                         >  2:235113/1‑150 (MQ=255)
                                                             cAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTAt                                                                                         >  2:527910/1‑150 (MQ=255)
                                                             cAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTAt                                                                                         <  1:1052288/150‑1 (MQ=255)
                                                             cAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTAt                                                                                         <  1:1420896/150‑1 (MQ=255)
                                                               aaCGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGa                                                                                       <  2:1526360/150‑1 (MQ=255)
                                                                  gCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGc                                                                                    >  2:1087532/1‑150 (MQ=255)
                                                                    tGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGccc                                                                                  <  2:479402/150‑1 (MQ=255)
                                                                    tGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTCTTTGCAAAAGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGcca                                                                                  >  2:925622/1‑149 (MQ=255)
                                                                       aaCATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTc                                                                               <  2:611230/150‑1 (MQ=255)
                                                                         cATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTcac                                                                             >  1:739087/1‑150 (MQ=255)
                                                                         cATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTcac                                                                             >  1:1364571/1‑150 (MQ=255)
                                                                         cATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTcac                                                                             <  1:931051/150‑1 (MQ=255)
                                                                          atgatgCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTcaca                                                                            <  1:235113/150‑1 (MQ=255)
                                                                           tgatgCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAg                                                                           <  2:590230/150‑1 (MQ=255)
                                                                                       cATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCTCTTCACAGAATTGTCCTTTc                                                               <  2:1011117/150‑1 (MQ=255)
                                                                                       cATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTc                                                               <  1:90131/150‑1 (MQ=255)
                                                                                       cATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTc                                                               <  1:487608/150‑1 (MQ=255)
                                                                                           gAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGa                                                           >  2:454899/1‑150 (MQ=255)
                                                                                               cGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTcc                                                       >  1:185412/1‑150 (MQ=255)
                                                                                                    gAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGtctc                                                  >  2:693693/1‑150 (MQ=255)
                                                                                                    gAAAGTCGCTCGTTACGTTAAAAATTGACCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGtctc                                                  <  2:34236/150‑1 (MQ=255)
                                                                                                       aGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTctctct                                               <  2:1364571/150‑1 (MQ=255)
                                                                                                       aGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTctctct                                               <  2:739087/150‑1 (MQ=255)
                                                                                                        gTCGCTCGTTACGTAAAAAATTGCCCGTTTGTGAACCACTTGGTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtg                                              <  1:871633/150‑1 (MQ=255)
                                                                                                           gCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtgatg                                           >  1:937197/1‑150 (MQ=255)
                                                                                                           gCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTGCACAGAATTGTCCTTTCACGATTCCGTCTCTCtgatg                                           >  2:97741/1‑150 (MQ=255)
                                                                                                             tCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTgatgat                                         <  1:1321752/150‑1 (MQ=255)
                                                                                                                    gttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtt                                  >  1:1389578/1‑150 (MQ=255)
                                                                                                                    gttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCCTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtt                                  >  1:515096/1‑150 (MQ=255)
                                                                                                                     ttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtta                                 >  1:528515/1‑150 (MQ=255)
                                                                                                                     ttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtta                                 >  2:616325/1‑150 (MQ=255)
                                                                                                                     ttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtta                                 >  1:749298/1‑150 (MQ=255)
                                                                                                                         aaaTTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGttaatta                             <  1:1522287/150‑1 (MQ=255)
                                                                                                                         aaaTTGCACGTTAGTGAACCTCTTGTTTGCAAAGGGGCATGACTCCTGACTTTTATTTCTGCCTATTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTCTCCTTTCACGATTCCGTCTCTCTGATGATTGATGttaatta                             <  2:935328/150‑1 (MQ=255)
                                                                                                                           aTTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAAc                           <  2:937197/150‑1 (MQ=255)
                                                                                                                            atGCCCGTTTGCAAACAAGTGGTTCACAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtta                                 <  1:1064672/142‑1 (MQ=38)
                                                                                                                            atGCCCGTTTGCAAACAAGTGGTTCACAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtta                                 >  2:1064672/2‑143 (MQ=38)
                                                                                                                             tGCCCGTTTGCAAACAAGTGGTTCACAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACaa                         >  2:742596/1‑150 (MQ=255)
                                                                                                                               cccGTTTGCAAACAAGTGGTTCACAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATg                       >  2:1083284/1‑150 (MQ=255)
                                                                                                                                ccGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGt                      >  2:1577596/1‑150 (MQ=255)
                                                                                                                                  gTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTAt                    <  2:1154034/150‑1 (MQ=255)
                                                                                                                                     tgtgAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCa                 >  1:201128/1‑150 (MQ=255)
                                                                                                                                      gtgAACCACTTGTTTGCAAACGGGCATGACTCCTTACTTTTATTTCTGCCGTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCAc                <  2:71054/150‑1 (MQ=255)
                                                                                                                                      gtgAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCAc                <  2:360281/150‑1 (MQ=255)
                                                                                                                                      gtgAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCAc                >  1:1036295/1‑150 (MQ=255)
                                                                                                                                       tgAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCAcc               >  2:1380181/1‑150 (MQ=255)
                                                                                                                                       tgAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCAcc               >  1:1012237/1‑150 (MQ=255)
                                                                                                                                         aaCCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGa             >  2:446853/1‑150 (MQ=255)
                                                                                                                                         aaCCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGa             >  2:154472/1‑150 (MQ=255)
                                                                                                                                                tGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTACCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGAaaacaaa      >  1:1087024/1‑150 (MQ=255)
                                                                                                                                                 gTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGAaaacaaac     <  2:267343/150‑1 (MQ=255)
                                                                                                                                                  tttGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTTCACTTGTTTTTATTAATCCATTGACAGAATTTTCCTTTCACGATTCCGTCTCTCTTATGATTGATGTTAATTAACAATGTAATCAACGAAtacaaaca    <  1:1132771/150‑1 (MQ=255)
                                                                                                                                                  tttGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGAAaacaaaca    <  2:326796/150‑1 (MQ=255)
                                                                                                                                                   ttGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACATAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGAAAACAAACat   <  1:1545867/150‑1 (MQ=255)
                                                                                                                                                    tGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGAAAACAAACata  >  1:1640364/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGAAAACAAACATA  >  NC_000913/1487915‑1488212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: