Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 114,511 A→C 5.3% intergenic (+24/+11) guaC → / ← hofC GMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913114,5110AC 5.3% 231.1 / 3.9 76intergenic (+24/+11)guaC/hofCGMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein
Reads supporting (aligned to +/- strand):  ref base A (41/31);  new base C (2/2);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

GATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCGCCAT  >  NC_000913/114362‑114659
                                                                                                                                                     |                                                                                                                                                    
gaTATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCAACTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCaa                                                                                                                                                      >  2:1119068/1‑150 (MQ=255)
      ttGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGcc                                                                                                                                                <  2:582190/150‑1 (MQ=255)
        gggCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAg                                                                                                                                              <  2:483929/150‑1 (MQ=255)
         ggcggcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGc                                                                                                                                             <  2:1549025/150‑1 (MQ=255)
          gcggcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCg                                                                                                                                            <  1:949490/150‑1 (MQ=255)
            ggcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGtt                                                                                                                                          >  1:522406/1‑150 (MQ=255)
             gcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTa                                                                                                                                         >  1:1665343/1‑150 (MQ=255)
               cTGCGTTCAGCTTGTACATACGGTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCAGCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatc                                                                                                                                       <  1:389491/150‑1 (MQ=255)
                 gCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatccc                                                                                                                                     <  1:1542978/150‑1 (MQ=255)
                 gCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCAGAAAGAGCTGACCAAGCGCACCACGATTATTCCTGTGCAGGAACAAGAAAACCGCATCTTCAACAACATGTAATCTCCCAACGCTATCGTGGAGCAACACGACAGCTTTatccc                                                                                                                                     >  2:1408068/1‑150 (MQ=255)
                     tCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatcccatcc                                                                                                                                 >  2:165023/1‑150 (MQ=255)
                      cAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatcccatccc                                                                                                                                <  1:162141/150‑1 (MQ=255)
                      cAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGGGGAGCAACACGCCAGCGTTatcccatccc                                                                                                                                <  1:755589/150‑1 (MQ=255)
                        gCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATATCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCAc                                                                                                                              >  1:1436080/1‑150 (MQ=255)
                         cTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCCCGTTTATTCGTGTGCAGGAACAAGACAACCGCATCGTCAACCACCTCTAATCTCCCAACCCTGGCGTGGAGCAACACGCCAGCGTAAACCCATCCAACt                                                                                                                             >  1:1123213/1‑150 (MQ=255)
                         cTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACt                                                                                                                             <  1:1161349/150‑1 (MQ=255)
                              aCATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCACCAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACCCGCCAGCGATATCCCATCCCACTcatcg                                                                                                                        >  1:333320/1‑150 (MQ=255)
                              aCATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcg                                                                                                                        >  1:675674/1‑150 (MQ=255)
                               cATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcgc                                                                                                                       >  1:161448/1‑150 (MQ=255)
                               cATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcgc                                                                                                                       >  1:218475/1‑150 (MQ=255)
                                  aCGTTGGGGCTTCACGCCTGAAAGAGCTGACGAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcgcatc                                                                                                                    <  1:254070/150‑1 (MQ=255)
                                   cGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTTCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcgcatcg                                                                                                                   <  2:126630/150‑1 (MQ=255)
                                         ggCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTaaa                                                                                                             >  1:1392035/1‑150 (MQ=255)
                                          gCTTCACGCCTGAAAGAGCTGCCCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCCCTCATCGCATCGCCTAAAt                                                                                                            >  2:1412102/1‑150 (MQ=255)
                                          gCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAAt                                                                                                            >  2:955770/1‑150 (MQ=255)
                                          gCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAAt                                                                                                            <  2:675674/150‑1 (MQ=255)
                                          gCTTCACGCCTGAAAGAGCTGACCAAGAGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTTGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAAt                                                                                                            <  1:94141/150‑1 (MQ=255)
                                           cTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTTATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCCTCCCACTCATCGCATCGCCTaaaag                                                                                                           >  1:245540/1‑148 (MQ=255)
                                           cTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATg                                                                                                           <  2:844037/150‑1 (MQ=255)
                                                cGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGaaaa                                                                                                      <  1:846924/150‑1 (MQ=255)
                                                  ccTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAAtt                                                                                                    <  1:1310219/150‑1 (MQ=255)
                                                  ccTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGAGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAAtt                                                                                                    <  1:1296957/150‑1 (MQ=255)
                                                     gAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGc                                                                                                 >  2:1375978/1‑150 (MQ=255)
                                                          agCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATa                                                                                            <  2:218475/150‑1 (MQ=255)
                                                           gCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATAc                                                                                           <  2:856185/150‑1 (MQ=255)
                                                              gACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                                        >  2:1154307/1‑150 (MQ=255)
                                                                 cAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGcc                                                                                     >  2:1488950/1‑150 (MQ=255)
                                                                    gcgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccacc                                                                                  <  2:392315/150‑1 (MQ=255)
                                                                     cgcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccacca                                                                                 >  2:1498497/1‑150 (MQ=255)
                                                                      gcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccaccac                                                                                <  1:1481522/150‑1 (MQ=255)
                                                                           acGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCg                                                                           <  1:165023/150‑1 (MQ=255)
                                                                                     gtgtGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaataat                                                                 >  2:6324/1‑150 (MQ=255)
                                                                                     gtgtGAAGTAACAAGAAAACCGAAGCTTCAACAACCTGTAATCTACCAACGCTGGCGTGGAGCAAAACGCCAGCGTTATACCATCCCAATCATCGCTTCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaataat                                                                 <  1:1408068/150‑1 (MQ=255)
                                                                                        tgCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtcc                                                              <  1:1563469/150‑1 (MQ=255)
                                                                                        tgCACGACCAAGAAAACCGCATCTTCAACAACCTGTATTCTCCCAACGCTGGCGTGCAGCAACACGCCCGCGTTATCCCATCCCACTCATCGCCTCGCCTAAATGGAACATTGGCAGATTCATAGCCACCACCCGCGGTCCAATAATtcc                                                              >  2:1416985/1‑150 (MQ=255)
                                                                                            ggAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTccc                                                          >  2:1234160/1‑150 (MQ=255)
                                                                                            ggAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTACTccc                                                          >  1:1518594/1‑150 (MQ=255)
                                                                                             gAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCg                                                         <  1:1154307/150‑1 (MQ=255)
                                                                                              aaCAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGt                                                        >  2:1187548/1‑150 (MQ=255)
                                                                                                cAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCAACGCCAAAATGGAAAAATGGCACATACATTGCCACCACCAGCGAACCAAAAATTCCTCCCGtaa                                                      >  1:1217526/1‑148 (MQ=255)
                                                                                                  aGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATg                                                    <  2:1392035/150‑1 (MQ=255)
                                                                                                   gAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCCGATACATTGCCACCACCAGCGTCCCAATAATTCCTCCCGTTATGa                                                   >  1:338360/1‑150 (MQ=255)
                                                                                                       aCCGCAACTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAg                                               <  1:1119068/150‑1 (MQ=255)
                                                                                                                 caacaaCCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCCCTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGt                                     <  2:411641/150‑1 (MQ=255)
                                                                                                                 caacaaCCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGt                                     >  2:1294709/1‑150 (MQ=255)
                                                                                                                 caacaaCCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGt                                     <  1:955770/150‑1 (MQ=255)
                                                                                                                  aacaacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTAGCAATAATTCCTCCCGTTATGATCAGCAACGCCGGtt                                    >  2:696030/1‑150 (MQ=255)
                                                                                                                   acaacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTc                                   >  2:1128544/1‑150 (MQ=255)
                                                                                                                       ccTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGt                               >  2:567684/1‑150 (MQ=255)
                                                                                                                         tGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTaa                             >  2:1672379/1‑150 (MQ=255)
                                                                                                                             aTCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCTTCGCCTAATTGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCt                         <  1:1156772/150‑1 (MQ=255)
                                                                                                                             aTCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCt                         >  2:137666/1‑150 (MQ=255)
                                                                                                                             aTCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCt                         <  1:1498497/150‑1 (MQ=255)
                                                                                                                                 cccAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGa                     >  2:1626179/1‑150 (MQ=255)
                                                                                                                                 cccAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGa                     <  2:161448/150‑1 (MQ=255)
                                                                                                                                   cAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCCTCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAgg                   >  1:150010/1‑150 (MQ=255)
                                                                                                                                      cGCTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTa                >  1:1410750/1‑150 (MQ=255)
                                                                                                                                      cGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTa                >  2:1517437/1‑150 (MQ=255)
                                                                                                                                       gCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTAt               >  1:1095170/1‑150 (MQ=255)
                                                                                                                                        cTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATc              >  1:531091/1‑150 (MQ=255)
                                                                                                                                        cTGGCGAGCAGCAACACGCCAGCGTTATCCCCTCCCACTCAACGCATAGCCTAAATGGAAATTTGGGAGATACATTGCCACAACCAGAGAACAAATAATTCCTCCCGTTTTGATCAGCAACTCCGGTTCCAGTAAAGCTTCGAGGTTATc              >  2:1130152/1‑150 (MQ=255)
                                                                                                                                          ggCGTGGAGCAACACGCCAGCGCTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCg            >  2:995063/1‑150 (MQ=255)
                                                                                                                                            cGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAgg                   >  1:627147/1‑141 (MQ=255)
                                                                                                                                            cGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAgg                   <  2:627147/141‑1 (MQ=255)
                                                                                                                                                gAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAgcg      >  2:1684213/1‑150 (MQ=255)
                                                                                                                                                gAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGCAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAgcg      >  2:1717985/1‑150 (MQ=255)
                                                                                                                                                   cAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCGCCa   >  2:619239/1‑150 (MQ=255)
                                                                                                                                                    aaCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCGCCAt  >  2:699706/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCGCCAT  >  NC_000913/114362‑114659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: