Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,557,347 A→G 5.5% L231P (CTG→CCG)  ddpF ← D,D‑dipeptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,557,3470AG 5.5% 265.8 / 5.0 91L231P (CTG→CCG) ddpFD,D‑dipeptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (43/43);  new base G (3/2);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

GGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGG  >  NC_000913/1557198‑1557496
                                                                                                                                                     |                                                                                                                                                     
ggCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCa                                                                                                                                                       >  1:1457638/1‑150 (MQ=255)
 gCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAg                                                                                                                                                      <  2:552514/150‑1 (MQ=255)
     cGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCAcc                                                                                                                                                  <  2:631699/150‑1 (MQ=255)
        tCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACctgc                                                                                                                                               <  1:1230278/150‑1 (MQ=255)
        gcGTAGAAAAAACAGCCTAGCGGCAACGTGCGGTTTCCCGGCAGATCCGTCTTCCGTAATGCCCATTCTTCCTCCCGCGGGTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGAGTATGTATGTGCAGGTGCTGACGGCACctgc                                                                                                                                               <  1:80063/149‑1 (MQ=255)
           tAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCtgctgc                                                                                                                                            <  1:597641/150‑1 (MQ=255)
            aGAAAAAACAGCCTTGCGGCAACGGGCGGTATCCCGGCAGATCCGTTTTACGTAATGCCCAATCTTCCTCGAGCTGTTTATCAATGGCGGGGAGGGAATCCAGCAATACTCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTgcg                                                                                                                                           <  2:343324/150‑1 (MQ=255)
             taaaataCAGCCTTGCGGCATCGTGTGGTTTCCCGGCAGTTCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGTATCCAGCAATAATCGGGTGTTTGGATGATCAGGTTCTGACGGCACctgcacttc                                                                                                                                          <  2:1396096/149‑6 (MQ=255)
              aaaaaaCAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTAAGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGcgcg                                                                                                                                         <  1:588561/150‑1 (MQ=255)
                aaaaCAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTCATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTc                                                                                                                                       <  1:1197849/150‑1 (MQ=255)
                 aaaCAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCg                                                                                                                                      >  2:1565715/1‑150 (MQ=255)
                  aaCAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGCTCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGc                                                                                                                                     >  1:1225059/1‑150 (MQ=255)
                  aaCAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGc                                                                                                                                     >  2:59315/1‑150 (MQ=255)
                   aCAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGcc                                                                                                                                    >  1:418640/1‑150 (MQ=255)
                       ccTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGACCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGt                                                                                                                                >  1:1191111/1‑150 (MQ=255)
                            cGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                                                                           >  2:741329/1‑150 (MQ=255)
                            cGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc                                                                                                                           >  2:63058/1‑150 (MQ=255)
                             ggCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACa                                                                                                                          >  1:1313344/1‑150 (MQ=255)
                               cAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGATGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAAt                                                                                                                        <  2:1325313/150‑1 (MQ=255)
                               cAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAAt                                                                                                                        >  1:665592/1‑150 (MQ=255)
                                  cGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTg                                                                                                                     >  1:674158/1‑150 (MQ=255)
                                   gTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGc                                                                                                                    >  1:485633/1‑150 (MQ=255)
                                    tGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGcc                                                                                                                   <  1:1248148/150‑1 (MQ=255)
                                      tgccgtCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCg                                                                                                                 >  1:1193518/6‑150 (MQ=255)
                                      cGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGTGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCg                                                                                                                 >  2:1682153/1‑150 (MQ=255)
                                        gTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgag                                                                                                               <  2:519869/150‑1 (MQ=255)
                                        gTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCgag                                                                                                               >  1:602980/1‑150 (MQ=255)
                                           tCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATa                                                                                                            <  1:1482309/150‑1 (MQ=255)
                                           tCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATa                                                                                                            >  2:1477938/1‑150 (MQ=255)
                                             ccGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                                                                          >  2:1129315/1‑150 (MQ=255)
                                             ccGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACa                                                                                                          <  1:1277727/150‑1 (MQ=255)
                                                  aGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCAcc                                                                                                     <  2:594401/150‑1 (MQ=255)
                                                    aTCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                                                                                   >  2:89947/1‑150 (MQ=255)
                                                    aTCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                                                                                   <  1:50066/150‑1 (MQ=255)
                                                     tCCGGTTTTCGTTCTGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGcc                                                                                                  <  2:1691170/150‑1 (MQ=255)
                                                       cGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAc                                                                                                <  1:741329/150‑1 (MQ=255)
                                                                 aaTGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTc                                                                                      <  1:59315/150‑1 (MQ=255)
                                                                 aaTGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGGGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTc                                                                                      <  1:1019601/150‑1 (MQ=255)
                                                                   tGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGGCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCat                                                                                    <  1:28748/150‑1 (MQ=255)
                                                                    gCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATAGCTCaaa                                                                                   >  2:1628751/1‑148 (MQ=255)
                                                                       cATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCCCCCGATCGCTCATATGa                                                                                >  1:677192/1‑150 (MQ=255)
                                                                       cATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCata                                                                                   >  2:980853/1‑147 (MQ=255)
                                                                       cATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCata                                                                                   <  1:980853/147‑1 (MQ=255)
                                                                        aTTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGAc                                                                               >  1:1434353/1‑150 (MQ=255)
                                                                         ttcttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACg                                                                              <  2:485633/150‑1 (MQ=255)
                                                                              cctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGGGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCa                                                                         <  2:148776/150‑1 (MQ=255)
                                                                               ctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAc                                                                        <  2:548265/150‑1 (MQ=255)
                                                                                 ccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACCTTCTGCCCGAGATACATCCCCGCCACCCGATCGCTCATATGACGTATAACCg                                                                      >  2:835267/1‑150 (MQ=255)
                                                                                       gTTTATCAATGGTGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAt                                                                <  1:1682153/150‑1 (MQ=255)
                                                                                        tttATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAtt                                                               >  1:764690/1‑150 (MQ=255)
                                                                                         ttATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAACCGATCGCTCATATGACGTATCTCCGAGACATtg                                                              >  2:1053275/1‑150 (MQ=255)
                                                                                          tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgt                                                             >  2:1715200/1‑150 (MQ=255)
                                                                                          tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgt                                                             >  1:711159/1‑150 (MQ=255)
                                                                                          tATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgt                                                             <  1:1565715/150‑1 (MQ=255)
                                                                                             cAATGGCGGGGAGGGAATCCCGCAATAATCGGGGGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg                                                          <  2:1062706/150‑1 (MQ=255)
                                                                                             cAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg                                                          <  2:1068400/150‑1 (MQ=255)
                                                                                             cAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg                                                          <  2:392530/150‑1 (MQ=255)
                                                                                              aaTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGa                                                         >  2:136189/1‑150 (MQ=255)
                                                                                                  gCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATc                                                     <  2:1313344/150‑1 (MQ=255)
                                                                                                   cGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCa                                                    >  2:1046117/1‑150 (MQ=255)
                                                                                                    ggggAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAg                                                   <  1:1295209/150‑1 (MQ=255)
                                                                                                    ggggAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAg                                                   >  2:33856/1‑150 (MQ=255)
                                                                                                    ggggAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAg                                                   <  2:1457638/150‑1 (MQ=255)
                                                                                                     gggagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGc                                                  <  1:929848/150‑1 (MQ=255)
                                                                                                          ggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATa                                             <  2:674158/150‑1 (MQ=255)
                                                                                                          ggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATa                                             <  1:926101/150‑1 (MQ=255)
                                                                                                             aTCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGt                                          >  1:875656/1‑150 (MQ=255)
                                                                                                              tCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTc                                         <  1:607038/150‑1 (MQ=255)
                                                                                                               ccAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCa                                        >  1:971393/1‑150 (MQ=255)
                                                                                                                cAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAg                                       >  2:1598573/1‑150 (MQ=255)
                                                                                                                  gCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGcc                                     <  2:1191111/150‑1 (MQ=255)
                                                                                                                   cAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCCCCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGCAATCAGCACATCGGTCAGccc                                    >  2:223455/1‑150 (MQ=255)
                                                                                                                   cAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGCTCGCGCCTATGACGTATCCCCGAGACAATGTGTGAACTCAGCACATAGGACAGcac                                    >  1:80579/1‑148 (MQ=255)
                                                                                                                       aatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGa                                <  1:1477938/150‑1 (MQ=255)
                                                                                                                             gtgtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTcc                          <  2:1619819/150‑1 (MQ=255)
                                                                                                                             gtgtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTcc                          >  2:856625/1‑150 (MQ=255)
                                                                                                                             gtgtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTcc                          <  1:1501350/150‑1 (MQ=255)
                                                                                                                              tgtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCt                         <  1:136189/150‑1 (MQ=255)
                                                                                                                                tATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGAGCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACAGAGGTCAGCCCGTGATTTTCCTGt                       >  1:908990/1‑150 (MQ=255)
                                                                                                                                      tgtgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATTAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGtt                 >  1:1310780/1‑150 (MQ=255)
                                                                                                                                      gcagCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGtt                 >  1:839324/4‑150 (MQ=255)
                                                                                                                                            ggTGCTGCCGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATAACCGCCACCCGATCTCTCATATGACGTATCACCGAGACATTGTGTGCAATCAGCACATAGGTCAGCCCGTGATTTTCCGGTAGCGTTACCAGt           >  2:1049376/1‑150 (MQ=255)
                                                                                                                                            ggTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGCGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGt           >  2:1043863/1‑150 (MQ=255)
                                                                                                                                            ggTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCCCCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGt           >  2:1099572/1‑150 (MQ=255)
                                                                                                                                            ggTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGt           >  2:1498110/1‑150 (MQ=255)
                                                                                                                                                ccGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAAt       <  2:1225059/150‑1 (MQ=255)
                                                                                                                                                ccGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAAt       >  1:1270393/1‑150 (MQ=255)
                                                                                                                                                 cGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAAtt      >  2:1560950/1‑150 (MQ=255)
                                                                                                                                                  gTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTg     >  1:670946/1‑150 (MQ=255)
                                                                                                                                                  gTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCGCATATGACGTAACACCGAGACATTGTGTGAAATCAGCACATAGGGCAGCCCGTGAGATTCCTGTAGCGTTACCAGTAAAATg     >  2:482645/1‑150 (MQ=255)
                                                                                                                                                  gTCAGCACCTGCTGCGCGTCGCCCAGGTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTg     <  1:1297741/150‑1 (MQ=255)
                                                                                                                                                    cAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAg   <  2:1193518/150‑1 (MQ=255)
                                                                                                                                                     aGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAgg  <  1:1324417/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGG  >  NC_000913/1557198‑1557496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: