Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,579,563 G→T 8.7% intergenic (‑221/+70) yddA ← / ← ydeM putative multidrug ABC transporter permease/ATPase/putative YdeN‑specific sulfatase‑maturating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,579,5630GT 8.7% 366.7 / 19.6 127intergenic (‑221/+70)yddA/ydeMputative multidrug ABC transporter permease/ATPase/putative YdeN‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base G (57/59);  new base T (4/7);  total (61/66)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01

Only 100 of 129 total aligned reads displayed.

CATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGCG  >  NC_000913/1579414‑1579711
                                                                                                                                                     |                                                                                                                                                    
cATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAg                                                                                                                                                      >  1:458372/1‑150 (MQ=255)
 aTTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAgg                                                                                                                                                     <  2:134488/150‑1 (MQ=255)
       cAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTa                                                                                                                                               <  1:1151488/150‑1 (MQ=255)
         atatGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATg                                                                                                                                             <  1:1594654/150‑1 (MQ=255)
          tatGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGt                                                                                                                                            <  2:458372/150‑1 (MQ=255)
           atGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGTTAATCAAGGATTTTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGAGGATGACGAACCACCCGACAGGAATTTATGTc                                                                                                                                           <  1:943519/150‑1 (MQ=255)
           atGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTTGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTc                                                                                                                                           <  2:1345549/150‑1 (MQ=255)
             gAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCgg                                                                                                                                         >  1:427167/1‑150 (MQ=255)
             gAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCATGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCgg                                                                                                                                         >  1:988656/1‑150 (MQ=255)
              aaaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCggg                                                                                                                                        <  2:1166943/150‑1 (MQ=255)
               aaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                                                                       <  1:1519591/150‑1 (MQ=255)
               aaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                                                                       <  2:927552/150‑1 (MQ=255)
                atatAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGTg                                                                                                                                      <  2:1520641/150‑1 (MQ=255)
                 tataAAGGAATATTTAATTTTCAGTGGTTATGGTTATGCTTTTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGTATTTTCGCAATGGAATATGATGATGACGAATCACCCGACATATATTGCTGTCGGGTgt                                                                                                                                     <  1:790087/150‑2 (MQ=255)
                  ataAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGTgtt                                                                                                                                    <  1:759071/150‑3 (MQ=255)
                  ataAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGcc                                                                                                                                    >  2:534164/1‑150 (MQ=255)
                   taAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGTgttt                                                                                                                                   <  2:836613/150‑4 (MQ=255)
                    aaaGGAATATTTCATTTTAAGTGTTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATTCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCtt                                                                                                                                  <  1:650350/150‑1 (MQ=255)
                      agtaaTATTTCATTTTCAGTGGGTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGt                                                                                                                                <  2:350478/147‑1 (MQ=255)
                       ggAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGtt                                                                                                                               <  1:799087/150‑1 (MQ=255)
                       ggAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCAGGTGCCTTGtt                                                                                                                               <  2:347808/150‑1 (MQ=255)
                         aaTATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTaa                                                                                                                             <  1:754590/150‑1 (MQ=255)
                          atatTTCATTTTCATTGGTTATGGTTTTGCTATAGATAATCAAGGATATTGCTAATTCATTGATGCATACCACGTATGCCAGGATTTTCGCAAGTGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAg                                                                                                                            <  1:285268/150‑1 (MQ=255)
                          atatTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAg                                                                                                                            <  1:824517/150‑1 (MQ=255)
                           tatTTCATTTTCAGTGGTTATGGTTATGCTATTGGTAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAgg                                                                                                                           <  2:1518383/150‑1 (MQ=255)
                           tatTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGAATACCCCGTAAGCCCGTATTTTCGCAAGGTAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGGGCCTTGTTAAgg                                                                                                                           <  2:904243/150‑1 (MQ=255)
                            atTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGt                                                                                                                          <  2:152084/150‑1 (MQ=255)
                            atTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGt                                                                                                                          <  2:690016/150‑1 (MQ=255)
                             tttCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTc                                                                                                                         <  1:475495/150‑1 (MQ=255)
                                cATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATa                                                                                                                      <  1:572782/150‑1 (MQ=255)
                                 aTTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaa                                                                                                                     >  1:1126820/1‑150 (MQ=255)
                                         tggttatggttaATCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAaggagg                                                                                                             <  2:404913/150‑1 (MQ=255)
                                          ggttatggttatGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGc                                                                                                            >  2:1305383/1‑150 (MQ=255)
                                          ggttatggttatGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGc                                                                                                            >  2:1652163/1‑150 (MQ=255)
                                          ggttatggttatGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGc                                                                                                            >  2:1712787/1‑150 (MQ=255)
                                           gttatggttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCt                                                                                                           >  1:1383648/1‑150 (MQ=255)
                                           gttatggttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCt                                                                                                           >  1:1266947/1‑150 (MQ=255)
                                                ggttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGCCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGaa                                                                                                      >  1:1016096/1‑150 (MQ=255)
                                                ggttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCTGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGaa                                                                                                      >  2:1095459/1‑150 (MQ=255)
                                                  ttatgCTTTTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATg                                                                                                    <  1:32362/150‑1 (MQ=255)
                                                  ttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAAtt                                                                                                    >  1:883014/1‑149 (MQ=255)
                                                   tatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATgg                                                                                                   <  1:91137/150‑1 (MQ=255)
                                                     tgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAg                                                                                                 <  2:1257807/150‑1 (MQ=255)
                                                     tgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAg                                                                                                 >  1:409212/1‑150 (MQ=255)
                                                     tgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAg                                                                                                 <  1:1141788/150‑1 (MQ=255)
                                                      gCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGt                                                                                                >  1:874688/1‑150 (MQ=255)
                                                       cTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGtt                                                                                               <  1:1652163/150‑1 (MQ=255)
                                                          ttGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTaaa                                                                                            <  1:889909/150‑1 (MQ=255)
                                                           tGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTaaaa                                                                                           >  1:434780/1‑150 (MQ=255)
                                                            gATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAag                                                                                          <  2:1244865/150‑1 (MQ=255)
                                                            gATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAag                                                                                          <  2:1620514/150‑1 (MQ=255)
                                                               aaTCAAGGATATTGCTAATGCCCTGATGCATACCACGAAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAagaga                                                                                       <  2:229149/150‑1 (MQ=255)
                                                                        tatTCCTAATGCCCTGATGCATACCACGTAAGCCAGGATATTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTATGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGttatta                                                                              <  2:485121/150‑1 (MQ=255)
                                                                          ttGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAACGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaat                                                                            <  2:823905/150‑1 (MQ=255)
                                                                           tGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatt                                                                           >  2:684186/1‑150 (MQ=255)
                                                                            gCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                          >  1:1136366/1‑150 (MQ=255)
                                                                             cTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGTAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAattat                                                                         >  2:1156468/1‑150 (MQ=255)
                                                                             cTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAattat                                                                         <  1:388233/150‑1 (MQ=255)
                                                                               aaTGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATtc                                                                       >  2:590829/1‑150 (MQ=255)
                                                                                aTGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATtct                                                                      >  1:25331/1‑150 (MQ=255)
                                                                                   cccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCa                                                                   >  1:1496356/1‑150 (MQ=255)
                                                                                     cTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAtt                                                                 >  2:1506094/1‑150 (MQ=255)
                                                                                          gCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGttt                                                            >  1:744202/1‑150 (MQ=255)
                                                                                             tACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGc                                                         <  1:1661739/150‑1 (MQ=255)
                                                                                              aCCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCa                                                        <  2:874688/150‑1 (MQ=255)
                                                                                              aCCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCa                                                        <  1:1712787/150‑1 (MQ=255)
                                                                                              aCCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCa                                                        >  1:710701/1‑150 (MQ=255)
                                                                                                cACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGGGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAAc                                                      >  1:269708/1‑150 (MQ=255)
                                                                                                cACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAAc                                                      >  2:1590783/1‑150 (MQ=255)
                                                                                                    tAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATcc                                                  <  2:434780/150‑1 (MQ=255)
                                                                                                       gCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATa                                               <  1:357340/150‑1 (MQ=255)
                                                                                                        ccAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATaa                                              >  1:1274206/1‑150 (MQ=255)
                                                                                                           ggATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAAtgt                                           >  1:860247/1‑150 (MQ=255)
                                                                                                             aTTTTCGGAAGGGAAGATGTTGATGACGAAACCCCCGACAGGAATTTATGTCGTGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTgg                                         <  1:174748/150‑1 (MQ=255)
                                                                                                              ttttCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGt                                        <  2:1139639/150‑1 (MQ=255)
                                                                                                              ttttCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGt                                        <  1:1305383/150‑1 (MQ=255)
                                                                                                                ttCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTaa                                      >  1:466338/1‑150 (MQ=255)
                                                                                                                  cGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGCGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAg                                    >  1:8571/1‑150 (MQ=255)
                                                                                                                     aaGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTCAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCgg                                 <  2:265361/150‑1 (MQ=255)
                                                                                                                      aGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGt                                >  1:1587320/1‑150 (MQ=255)
                                                                                                                         gacattgatgatgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTAct                             >  1:1114603/6‑150 (MQ=255)
                                                                                                                          aaGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTActc                            <  2:1383648/150‑1 (MQ=255)
                                                                                                                          aaGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTActc                            >  1:1545298/1‑150 (MQ=255)
                                                                                                                           aGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTActct                           >  2:526083/1‑150 (MQ=255)
                                                                                                                            gatgatgatGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTg                          >  2:324593/1‑150 (MQ=255)
                                                                                                                                atgatgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCt                      >  1:811640/1‑150 (MQ=255)
                                                                                                                                 tgatgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCtt                     <  2:818891/150‑1 (MQ=255)
                                                                                                                                  gatgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTa                    <  1:819354/150‑1 (MQ=255)
                                                                                                                                    tgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGc                  >  1:978116/1‑150 (MQ=255)
                                                                                                                                     gaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCt                 >  1:1072785/1‑150 (MQ=255)
                                                                                                                                          ggcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAActc            >  1:205779/3‑150 (MQ=255)
                                                                                                                                           gcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAActct           >  1:67345/2‑150 (MQ=255)
                                                                                                                                            cacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTa          >  2:1433013/1‑150 (MQ=255)
                                                                                                                                            cacCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGGGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTa          <  2:1126820/150‑1 (MQ=255)
                                                                                                                                              cccGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTATAGCGGTACTCTGTTCTTAGCTAACTCTAcc        >  2:497407/1‑150 (MQ=255)
                                                                                                                                              cccGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCATAGCTAACTCTAcc        >  1:1449792/1‑150 (MQ=255)
                                                                                                                                                cGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCAt      <  2:1372718/150‑1 (MQ=255)
                                                                                                                                                 gACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATg     >  2:162918/1‑150 (MQ=255)
                                                                                                                                                  aCAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATgg    <  1:1500793/150‑1 (MQ=255)
                                                                                                                                                    aGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGCg  <  2:860247/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGCG  >  NC_000913/1579414‑1579711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: