Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,596,499 C→A 5.7% pseudogene (1489/3861 nt) yneO ← pseudogene, AidA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,596,4990CA 5.7% 312.7 / 5.4 100pseudogene (1489/3861 nt)yneOpseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base C (44/50);  new base A (2/4);  total (46/54)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 104 total aligned reads displayed.

TGTATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTG  >  NC_000913/1596350‑1596648
                                                                                                                                                     |                                                                                                                                                     
tgtATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGc                                                                                                                                                       <  2:178259/150‑1 (MQ=255)
tgtATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGc                                                                                                                                                       <  1:914705/150‑1 (MQ=255)
 gtATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATTCACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCa                                                                                                                                                      >  2:1374812/1‑150 (MQ=255)
 gtATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCa                                                                                                                                                      >  2:228095/1‑150 (MQ=255)
  tATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTACCGGAGGCAtt                                                                                                                                                    <  2:462012/150‑1 (MQ=255)
    tCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCAttt                                                                                                                                                   <  2:1435452/150‑1 (MQ=255)
     cACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtc                                                                                                                                                  <  1:1398072/150‑1 (MQ=255)
      aCGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcc                                                                                                                                                 <  2:1370055/150‑1 (MQ=255)
      aCGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcc                                                                                                                                                 <  1:615220/150‑1 (MQ=255)
       cgcgCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcct                                                                                                                                                >  1:1176499/1‑150 (MQ=255)
         cgcTTTAACTATACCTCCGGTGTTAATATTCAGTTCACCCGTGACTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGATATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                              <  1:221745/150‑1 (MQ=255)
         cgcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                              <  2:1354936/150‑1 (MQ=255)
         cgcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                              <  2:229194/150‑1 (MQ=255)
          gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                             <  2:555897/150‑1 (MQ=255)
          gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                             <  1:1271347/150‑1 (MQ=255)
          gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                             <  1:487911/150‑1 (MQ=255)
          gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACACGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                             <  1:1178538/150‑1 (MQ=255)
            tttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACCCCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTATCGGAGGAAATGCCTCCggt                                                                                                                                           <  2:114356/150‑3 (MQ=255)
            tttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGAt                                                                                                                                           >  1:1014878/1‑150 (MQ=255)
            tttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGAt                                                                                                                                           <  2:409838/150‑1 (MQ=255)
            tttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGAAATGCCTCCggt                                                                                                                                           <  1:614521/150‑3 (MQ=255)
             ttACCTCTACCTCCGGTTGTAATATTCAGTTCACCCGTGCCTAATTCTCCGACCTGTAGCATTTGTGCGGTAGTAGATGATTTCTGTAAATTCCAGAGACTGTGCGTTGAAATATTAACTATCCCCTTACCGGAGGAAATGCCTCCGGTa                                                                                                                                          <  2:679764/150‑1 (MQ=255)
                  tataCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATcc                                                                                                                                     <  2:639994/150‑1 (MQ=255)
                  aataCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATcc                                                                                                                                     <  2:1310364/149‑1 (MQ=255)
                    taCCTCCGGTGGTAATATTCTGTTCACGCGTGCCTAATACACCGACCTGTAGCATTTGTGCGTTAGTAGATGATGTCTTTAAATTGCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCAt                                                                                                                                   <  2:385011/150‑1 (MQ=255)
                     aCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCAGATAAATCCATa                                                                                                                                  >  1:1435994/1‑150 (MQ=255)
                       cTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTACATGATGTCTTTAAATTCCAGCGACTGTCCGTTGAAATATTAACAATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGc                                                                                                                                <  2:1306537/150‑1 (MQ=255)
                         ccGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGccc                                                                                                                              >  2:133610/1‑150 (MQ=255)
                            gtggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGtt                                                                                                                           <  2:1371954/150‑1 (MQ=255)
                            gtggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGtt                                                                                                                           >  2:1294584/1‑150 (MQ=255)
                             tggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTg                                                                                                                          <  1:1314785/150‑1 (MQ=255)
                             tggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTg                                                                                                                          <  1:1445047/150‑1 (MQ=255)
                                tAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAt                                                                                                                       <  1:426547/150‑1 (MQ=255)
                                tAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAt                                                                                                                       >  2:1301338/1‑150 (MQ=255)
                                tAATAGTCAGTTCACCCGGGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAt                                                                                                                       <  2:50966/150‑1 (MQ=255)
                                     ttCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAgg                                                                                                                  <  1:1632539/150‑1 (MQ=255)
                                        aGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATAAAGGGTg                                                                                                               <  2:1164281/150‑1 (MQ=255)
                                         gTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTgg                                                                                                              >  1:531253/1‑150 (MQ=255)
                                            cACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCAc                                                                                                           <  1:549206/150‑1 (MQ=255)
                                             aCCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGAAcc                                                                                                          >  1:822801/1‑150 (MQ=255)
                                              cccGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCa                                                                                                         >  1:531735/1‑150 (MQ=255)
                                                cGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCAtt                                                                                                       >  1:1509798/1‑150 (MQ=255)
                                                 gTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTa                                                                                                      <  1:142014/150‑1 (MQ=255)
                                                      tAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGAc                                                                                                 >  2:515373/1‑150 (MQ=255)
                                                           cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTa                                                                                            >  1:1182041/1‑150 (MQ=255)
                                                           cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATACATAGACCGTTGAATTAAGGGTGGAACCATTACTGACATTTa                                                                                            >  2:50423/1‑150 (MQ=255)
                                                                 ccTGTAGCAATTGTGTGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATAc                                                                                      >  2:289553/1‑150 (MQ=255)
                                                                 ccTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATAc                                                                                      <  2:422918/150‑1 (MQ=255)
                                                                  cTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATAcc                                                                                     <  2:1058980/150‑1 (MQ=255)
                                                                       gCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTAc                                                                                >  2:1676439/1‑150 (MQ=255)
                                                                       gCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTAc                                                                                <  2:372894/150‑1 (MQ=255)
                                                                            tgtgCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGATACCaaaa                                                                           >  2:1068039/1‑150 (MQ=255)
                                                                               gCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTa                                                                        >  2:1720223/1‑150 (MQ=255)
                                                                                cGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCAATAGCACGTTGAATTAAGGGTTGCACCATTACTGACATTTACGATACCGCTACCAAAATTAc                                                                       >  1:1434258/1‑150 (MQ=255)
                                                                                 gTTTGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCAGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACAATTACGATACCGCTACCAAAATTAcc                                                                      >  1:1430603/1‑150 (MQ=255)
                                                                                 gTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTAcc                                                                      >  2:376176/1‑150 (MQ=255)
                                                                                    agtagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGAc                                                                   <  2:1529552/150‑1 (MQ=255)
                                                                                     ttagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACa                                                                  <  1:496880/149‑1 (MQ=255)
                                                                                      tagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAt                                                                 >  2:1175185/1‑150 (MQ=255)
                                                                                       agATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCATTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAtt                                                                >  2:204308/1‑150 (MQ=255)
                                                                                            atgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAc                                                           <  2:985200/150‑1 (MQ=255)
                                                                                             tgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAcc                                                          <  2:531735/150‑1 (MQ=255)
                                                                                               tCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGc                                                                                   >  1:551712/1‑123 (MQ=255)
                                                                                               tCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGc                                                                                   <  2:551712/123‑1 (MQ=255)
                                                                                                cTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCcac                                                       <  1:1417136/150‑1 (MQ=255)
                                                                                                cTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCcac                                                       >  2:1408475/1‑150 (MQ=255)
                                                                                                   tAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGt                                                    >  1:1669198/1‑150 (MQ=255)
                                                                                                      aTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACAAAAATTACCGACATTGATACCCACAGTATc                                                 >  2:1552086/1‑150 (MQ=255)
                                                                                                      aTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACAGTAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAATGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTAACGACATTGATACCCACAGAATc                                                 >  1:1058352/1‑150 (MQ=255)
                                                                                                       ttCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCg                                                <  1:1101033/150‑1 (MQ=255)
                                                                                                          cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAc                                             >  2:1405873/1‑150 (MQ=255)
                                                                                                          cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAc                                             >  2:212562/1‑150 (MQ=255)
                                                                                                           agagACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACg                                            >  2:1406191/1‑150 (MQ=255)
                                                                                                           agagACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACg                                            <  1:1720223/150‑1 (MQ=255)
                                                                                                            gagaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACga                                           <  2:1323740/150‑1 (MQ=255)
                                                                                                             agaCTGTCCGTTGAAATATTAACTATCCCCTAACCGGAGGCATTTCCTCCGATAAATCCTTAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgat                                          <  2:666657/150‑1 (MQ=255)
                                                                                                              gaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGCCATTTACGCTACCGCTACCAAAATTACCGACATTGATAACCACAGTATCGAACgaag                                         >  2:253003/1‑148 (MQ=255)
                                                                                                              gaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatg                                         >  2:25080/1‑150 (MQ=255)
                                                                                                              gaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatg                                         <  2:1509798/150‑1 (MQ=255)
                                                                                                                          aaaTATTAACTATCCCATTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatcca                             <  1:50423/150‑1 (MQ=255)
                                                                                                                             tatTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCa                          <  1:133610/150‑1 (MQ=255)
                                                                                                                             tatTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCa                          >  2:1034322/1‑150 (MQ=255)
                                                                                                                              atTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAg                         >  1:422179/1‑150 (MQ=255)
                                                                                                                               ttAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAgg                        <  1:988073/150‑1 (MQ=255)
                                                                                                                                  aCTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTc                     <  1:1408475/150‑1 (MQ=255)
                                                                                                                                   cTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCa                    >  1:1438692/1‑150 (MQ=255)
                                                                                                                                      tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                 <  1:692423/150‑1 (MQ=255)
                                                                                                                                      tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                 <  1:25080/150‑1 (MQ=255)
                                                                                                                                      tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                 <  1:1033346/150‑1 (MQ=255)
                                                                                                                                        cccTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCCCCATTACTGACATTTACGATACAGCTACCAAATTTACCGACATTGATACCCAAAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGtgtg               >  1:299222/1‑150 (MQ=255)
                                                                                                                                          attACCGTAGGAATTTCCTCAGATAAATCCATAGAACGTTGAATTAAGTGTGGCACAATTACTTACATTTTCGATACAGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGATCAGTGTGcc             <  1:80290/149‑1 (MQ=255)
                                                                                                                                           ttACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCg            <  1:953199/150‑1 (MQ=255)
                                                                                                                                            tatcGGAGGAAATGCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAATTTACCGACAATGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGa           >  1:290466/4‑150 (MQ=255)
                                                                                                                                            tACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGa           >  1:593621/1‑150 (MQ=255)
                                                                                                                                             atcGGAGGAAATGCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGAt          >  1:1010610/3‑150 (MQ=255)
                                                                                                                                              ccGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATa         >  2:1608247/1‑150 (MQ=255)
                                                                                                                                              ccGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATa         <  2:1014878/150‑1 (MQ=255)
                                                                                                                                                ggaggaAATGCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATAcc       >  1:1547045/1‑150 (MQ=255)
                                                                                                                                                  aGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTTCCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgc     >  1:454101/1‑150 (MQ=255)
                                                                                                                                                     cATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGTTTCAGTGTGCCTATACCgcttg  >  1:151686/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTG  >  NC_000913/1596350‑1596648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: