Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,596,501 T→A 5.8% pseudogene (1487/3861 nt) yneO ← pseudogene, AidA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,596,5010TA 5.8% 292.1 / 3.3 98pseudogene (1487/3861 nt)yneOpseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base T (43/49);  new base A (3/3);  total (46/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCT  >  NC_000913/1596352‑1596650
                                                                                                                                                     |                                                                                                                                                     
tATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTACCGGAGGCAtt                                                                                                                                                      <  2:462012/150‑1 (MQ=255)
  tCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCAttt                                                                                                                                                     <  2:1435452/150‑1 (MQ=255)
   cACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtc                                                                                                                                                    <  1:1398072/150‑1 (MQ=255)
    aCGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcc                                                                                                                                                   <  1:615220/150‑1 (MQ=255)
    aCGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcc                                                                                                                                                   <  2:1370055/150‑1 (MQ=255)
     cgcgCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcct                                                                                                                                                  >  1:1176499/1‑150 (MQ=255)
       cgcTTTAACTATACCTCCGGTGTTAATATTCAGTTCACCCGTGACTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGATATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                                <  1:221745/150‑1 (MQ=255)
       cgcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                                <  2:1354936/150‑1 (MQ=255)
       cgcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                                <  2:229194/150‑1 (MQ=255)
        gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                               <  1:1271347/150‑1 (MQ=255)
        gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                               <  1:487911/150‑1 (MQ=255)
        gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                               <  2:555897/150‑1 (MQ=255)
        gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACACGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                               <  1:1178538/150‑1 (MQ=255)
          tttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACCCCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTATCGGAGGAAATGCCTCCggt                                                                                                                                             <  2:114356/150‑3 (MQ=255)
          tttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGAt                                                                                                                                             >  1:1014878/1‑150 (MQ=255)
          tttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGAt                                                                                                                                             <  2:409838/150‑1 (MQ=255)
          tttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGAAATGCCTCCggt                                                                                                                                             <  1:614521/150‑3 (MQ=255)
           ttACCTCTACCTCCGGTTGTAATATTCAGTTCACCCGTGCCTAATTCTCCGACCTGTAGCATTTGTGCGGTAGTAGATGATTTCTGTAAATTCCAGAGACTGTGCGTTGAAATATTAACTATCCCCTTACCGGAGGAAATGCCTCCGGTa                                                                                                                                            <  2:679764/150‑1 (MQ=255)
                tataCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATcc                                                                                                                                       <  2:639994/150‑1 (MQ=255)
                aataCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATcc                                                                                                                                       <  2:1310364/149‑1 (MQ=255)
                  taCCTCCGGTGGTAATATTCTGTTCACGCGTGCCTAATACACCGACCTGTAGCATTTGTGCGTTAGTAGATGATGTCTTTAAATTGCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCAt                                                                                                                                     <  2:385011/150‑1 (MQ=255)
                   aCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCAGATAAATCCATa                                                                                                                                    >  1:1435994/1‑150 (MQ=255)
                     cTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTACATGATGTCTTTAAATTCCAGCGACTGTCCGTTGAAATATTAACAATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGc                                                                                                                                  <  2:1306537/150‑1 (MQ=255)
                       ccGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGccc                                                                                                                                >  2:133610/1‑150 (MQ=255)
                          gtggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGtt                                                                                                                             <  2:1371954/150‑1 (MQ=255)
                          gtggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGtt                                                                                                                             >  2:1294584/1‑150 (MQ=255)
                           tggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTg                                                                                                                            <  1:1314785/150‑1 (MQ=255)
                           tggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTg                                                                                                                            <  1:1445047/150‑1 (MQ=255)
                              tAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAt                                                                                                                         >  2:1301338/1‑150 (MQ=255)
                              tAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAt                                                                                                                         <  1:426547/150‑1 (MQ=255)
                              tAATAGTCAGTTCACCCGGGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAt                                                                                                                         <  2:50966/150‑1 (MQ=255)
                                   ttCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAgg                                                                                                                    <  1:1632539/150‑1 (MQ=255)
                                      aGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTg                                                                                                                 >  2:1713208/1‑150 (MQ=255)
                                      aGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATAAAGGGTg                                                                                                                 <  2:1164281/150‑1 (MQ=255)
                                       gTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTgg                                                                                                                >  1:531253/1‑150 (MQ=255)
                                          cACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCAc                                                                                                             <  1:549206/150‑1 (MQ=255)
                                           aCCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGAAcc                                                                                                            >  1:822801/1‑150 (MQ=255)
                                            cccGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCa                                                                                                           >  1:531735/1‑150 (MQ=255)
                                              cGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCAtt                                                                                                         >  1:1509798/1‑150 (MQ=255)
                                               gTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTa                                                                                                        <  1:142014/150‑1 (MQ=255)
                                                    tAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGAc                                                                                                   >  2:515373/1‑150 (MQ=255)
                                                         cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTa                                                                                              >  1:1182041/1‑150 (MQ=255)
                                                         cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATACATAGACCGTTGAATTAAGGGTGGAACCATTACTGACATTTa                                                                                              >  2:50423/1‑150 (MQ=255)
                                                               ccTGTAGCAATTGTGTGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATAc                                                                                        >  2:289553/1‑150 (MQ=255)
                                                               ccTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATAc                                                                                        <  2:422918/150‑1 (MQ=255)
                                                                cTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATAcc                                                                                       <  2:1058980/150‑1 (MQ=255)
                                                                     gCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTAc                                                                                  >  2:1676439/1‑150 (MQ=255)
                                                                     gCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTAc                                                                                  <  2:372894/150‑1 (MQ=255)
                                                                          tgtgCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGATACCaaaa                                                                             >  2:1068039/1‑150 (MQ=255)
                                                                           gtgCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAAt                                                                            <  1:1713208/150‑1 (MQ=255)
                                                                             gCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTa                                                                          >  2:1720223/1‑150 (MQ=255)
                                                                              cGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCAATAGCACGTTGAATTAAGGGTTGCACCATTACTGACATTTACGATACCGCTACCAAAATTAc                                                                         >  1:1434258/1‑150 (MQ=255)
                                                                               gTTTGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCAGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACAATTACGATACCGCTACCAAAATTAcc                                                                        >  1:1430603/1‑150 (MQ=255)
                                                                               gTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTAcc                                                                        >  2:376176/1‑150 (MQ=255)
                                                                                  agtagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGAc                                                                     <  2:1529552/150‑1 (MQ=255)
                                                                                   ttagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACa                                                                    <  1:496880/149‑1 (MQ=255)
                                                                                    tagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAt                                                                   >  2:1175185/1‑150 (MQ=255)
                                                                                     agATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCATTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAtt                                                                  >  2:204308/1‑150 (MQ=255)
                                                                                          atgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAc                                                             <  2:985200/150‑1 (MQ=255)
                                                                                           tgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAcc                                                            <  2:531735/150‑1 (MQ=255)
                                                                                             tCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGc                                                                                     >  1:551712/1‑123 (MQ=255)
                                                                                             tCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGc                                                                                     <  2:551712/123‑1 (MQ=255)
                                                                                              cTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCcac                                                         <  1:1417136/150‑1 (MQ=255)
                                                                                              cTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCcac                                                         >  2:1408475/1‑150 (MQ=255)
                                                                                                 tAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGt                                                      >  1:1669198/1‑150 (MQ=255)
                                                                                                    aTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACAAAAATTACCGACATTGATACCCACAGTATc                                                   >  2:1552086/1‑150 (MQ=255)
                                                                                                    aTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACAGTAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAATGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTAACGACATTGATACCCACAGAATc                                                   >  1:1058352/1‑150 (MQ=255)
                                                                                                     ttCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCg                                                  <  1:1101033/150‑1 (MQ=255)
                                                                                                        cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAc                                               >  2:1405873/1‑150 (MQ=255)
                                                                                                        cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAc                                               >  2:212562/1‑150 (MQ=255)
                                                                                                         agagACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACg                                              <  1:1720223/150‑1 (MQ=255)
                                                                                                         agagACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACg                                              >  2:1406191/1‑150 (MQ=255)
                                                                                                          gagaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACga                                             <  2:1323740/150‑1 (MQ=255)
                                                                                                           agaCTGTCCGTTGAAATATTAACTATCCCCTAACCGGAGGCATTTCCTCCGATAAATCCTTAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgat                                            <  2:666657/150‑1 (MQ=255)
                                                                                                            gaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGCCATTTACGCTACCGCTACCAAAATTACCGACATTGATAACCACAGTATCGAACgaag                                           >  2:253003/1‑148 (MQ=255)
                                                                                                            gaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatg                                           >  2:25080/1‑150 (MQ=255)
                                                                                                            gaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatg                                           <  2:1509798/150‑1 (MQ=255)
                                                                                                                        aaaTATTAACTATCCCATTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatcca                               <  1:50423/150‑1 (MQ=255)
                                                                                                                           tatTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCa                            <  1:133610/150‑1 (MQ=255)
                                                                                                                           tatTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCa                            >  2:1034322/1‑150 (MQ=255)
                                                                                                                            atTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAg                           >  1:422179/1‑150 (MQ=255)
                                                                                                                             ttAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAgg                          <  1:988073/150‑1 (MQ=255)
                                                                                                                                aCTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTc                       <  1:1408475/150‑1 (MQ=255)
                                                                                                                                 cTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCa                      >  1:1438692/1‑150 (MQ=255)
                                                                                                                                    tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                   <  1:692423/150‑1 (MQ=255)
                                                                                                                                    tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                   <  1:25080/150‑1 (MQ=255)
                                                                                                                                    tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                   <  1:1033346/150‑1 (MQ=255)
                                                                                                                                      cccTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCCCCATTACTGACATTTACGATACAGCTACCAAATTTACCGACATTGATACCCAAAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGtgtg                 >  1:299222/1‑150 (MQ=255)
                                                                                                                                        attACCGTAGGAATTTCCTCAGATAAATCCATAGAACGTTGAATTAAGTGTGGCACAATTACTTACATTTTCGATACAGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGATCAGTGTGcc               <  1:80290/149‑1 (MQ=255)
                                                                                                                                         ttACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCg              <  1:953199/150‑1 (MQ=255)
                                                                                                                                          tatcGGAGGAAATGCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAATTTACCGACAATGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGa             >  1:290466/4‑150 (MQ=255)
                                                                                                                                          tACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGa             >  1:593621/1‑150 (MQ=255)
                                                                                                                                           atcGGAGGAAATGCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGAt            >  1:1010610/3‑150 (MQ=255)
                                                                                                                                            ccGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATa           >  2:1608247/1‑150 (MQ=255)
                                                                                                                                            ccGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATa           <  2:1014878/150‑1 (MQ=255)
                                                                                                                                              ggaggaAATGCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATAcc         >  1:1547045/1‑150 (MQ=255)
                                                                                                                                                aGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTTCCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgc       >  1:454101/1‑150 (MQ=255)
                                                                                                                                                 ggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgct      >  2:1023243/1‑150 (MQ=255)
                                                                                                                                                   cATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGTTTCAGTGTGCCTATACCgcttg    >  1:151686/1‑150 (MQ=255)
                                                                                                                                                     tttCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttgct  <  1:515373/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCT  >  NC_000913/1596352‑1596650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: