Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,629,437 T→C 8.1% intergenic (+19/+16) ydfZ → / ← ydfI selenoprotein, function unknown/putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,629,4370TC 8.1% 204.2 / 8.2 74intergenic (+19/+16)ydfZ/ydfIselenoprotein, function unknown/putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (36/32);  new base C (3/3);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

GCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCC  >  NC_000913/1629291‑1629582
                                                                                                                                                  |                                                                                                                                                 
gCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggc                                                                                                                                                <  1:1394012/150‑1 (MQ=255)
 cAAGATCCTGTCGATTGATACTGAAGGTCTGCCCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTAGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggcg                                                                                                                                               >  1:1199207/1‑150 (MQ=255)
 cAAGATCCTGTCGATACATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGATGTTGAAGGTAGTGAAGAGAAACTGGCACCACGGGCCCTGATTCGACTCGGCAGGAACTAAGCGTGTGAAAGCCGCCGATggag                                                                                                                                               >  2:35257/1‑148 (MQ=255)
 cAAGATCCTGTCGAGTGATAATGAAGGTCTGACCGCTGAGCAAATGCGCCGCTGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCCCCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggcg                                                                                                                                               <  2:256878/150‑1 (MQ=255)
  aaGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAAGTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggcgg                                                                                                                                              <  1:1026179/150‑1 (MQ=255)
  aaGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggcgg                                                                                                                                              <  1:1196046/150‑1 (MQ=255)
   aGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggcggc                                                                                                                                             <  2:993829/150‑1 (MQ=255)
      tCCTGTCGCTTGATCCTGACGGGCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTGGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGCTTCGTCTCGGCATGACCTAAGCGTGTGAATGCCGCCATCGGCGGCAtt                                                                                                                                          <  1:81049/150‑1 (MQ=255)
      tCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCATCGGCGGCAtt                                                                                                                                          <  1:1112375/150‑1 (MQ=255)
       ccTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCATCGGCGGCAttc                                                                                                                                         <  2:667935/150‑2 (MQ=255)
             gATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCttttt                                                                                                                                   <  2:92968/150‑1 (MQ=255)
              aTTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCtttttt                                                                                                                                  <  1:806314/150‑1 (MQ=255)
              aTTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCtttttt                                                                                                                                  <  2:362946/150‑1 (MQ=255)
                            tCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGCTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGAATGAAATAAGCGTGTGAATGCCGCCGATGACGGCATGGCTTTTTTACTTCACGGAatat                                                                                                                    >  2:146446/1‑150 (MQ=255)
                               gACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATAtttt                                                                                                                 >  1:393245/1‑150 (MQ=255)
                               gACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATAtttt                                                                                                                 >  1:254038/1‑150 (MQ=255)
                                aCCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATTGCGGCATTGCTTTTTTACTTCACGGAATATTTTg                                                                                                                <  1:1098396/150‑1 (MQ=255)
                                 ccGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGc                                                                                                               <  2:1174207/150‑1 (MQ=255)
                                    cTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCAc                                                                                                            >  1:583734/1‑150 (MQ=255)
                                      gAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTAGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACgg                                                                                                          >  1:1551214/1‑150 (MQ=255)
                                       aGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGt                                                                                                         >  2:598412/1‑150 (MQ=255)
                                        gCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTc                                                                                                        >  2:828639/1‑150 (MQ=255)
                                          aaaTCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTCATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTATTACTTCACGGAATATTTAGCCACGGTCGc                                                                                                      >  2:181958/1‑150 (MQ=255)
                                          aaaTCCGCCGCGGAAAAACCGTAGTTGGGGAAGGTTGAGACGCGAAACTGGCCCCACTCGCCCTGATTCGTCGCGGCATGAACTAACCGTGTGAAAGCCGCCGCTGGCGGCCGTGCTGGTTTACATCACGGAATATGTTGCCACAGTCGc                                                                                                      >  1:120421/1‑150 (MQ=255)
                                            aTCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCtt                                                                                                    >  1:25307/1‑150 (MQ=255)
                                               cgccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcg                                                                                                 >  1:209990/1‑150 (MQ=255)
                                                gccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgc                                                                                                <  2:867201/150‑1 (MQ=255)
                                                 ccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgcg                                                                                               >  1:1243575/1‑150 (MQ=255)
                                                 ccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgcg                                                                                               >  2:697865/1‑150 (MQ=255)
                                                 ccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgcg                                                                                               >  1:797021/1‑150 (MQ=255)
                                                 ccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcgcg                                                                                               <  1:1247441/150‑1 (MQ=255)
                                                   gcgGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTAGCTTTcgcgac                                                                                             >  2:300803/1‑148 (MQ=255)
                                                   gcgGAAAAACCGTAGTTGTTGAAGGGTGGGAAGAGAAACTGGCACGACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGcc                                                                                             <  2:1565282/150‑1 (MQ=255)
                                                      gAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATg                                                                                          >  2:259982/1‑150 (MQ=255)
                                                        aaaaCCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATgcg                                                                                        >  2:565955/1‑150 (MQ=255)
                                                          aaCCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCt                                                                                      >  2:220436/1‑150 (MQ=255)
                                                          aaCCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCt                                                                                      >  1:1700040/1‑150 (MQ=255)
                                                                  ttgttgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGa                                                                              <  2:428616/150‑1 (MQ=255)
                                                                  ttgttgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGa                                                                              <  2:1262840/150‑1 (MQ=255)
                                                                   tgttgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGAc                                                                             <  1:1009967/150‑1 (MQ=255)
                                                                      tgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAg                                                                          >  1:99194/1‑150 (MQ=255)
                                                                        aaGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTa                                                                        <  1:697865/150‑1 (MQ=255)
                                                                        aaGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTa                                                                        >  1:705501/1‑150 (MQ=255)
                                                                          ggTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACg                                                                      >  2:1138210/1‑150 (MQ=255)
                                                                              gtgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTc                                                                  <  1:493858/150‑1 (MQ=255)
                                                                              gtgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGGTTc                                                                  >  2:40838/1‑150 (MQ=255)
                                                                                  agagAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTc                                                              <  2:705501/150‑1 (MQ=255)
                                                                                     gaAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCAct                                                           >  2:756535/1‑150 (MQ=255)
                                                                                       aaCTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCActct                                                         >  1:1532218/1‑150 (MQ=255)
                                                                                         cTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTg                                                       <  2:665319/150‑1 (MQ=255)
                                                                                          tGGCACCACTGGACCTGATGCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTGTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGc                                                      <  2:1561220/150‑1 (MQ=255)
                                                                                            gCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAg                                                    <  2:797021/150‑1 (MQ=255)
                                                                                            gCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAg                                                    <  2:1243575/150‑1 (MQ=255)
                                                                                                    ggACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaa                                            >  1:964128/1‑150 (MQ=255)
                                                                                                    ggACCTGATTCGTCTCGGCATGAACTAAGCGGGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaa                                            <  2:142073/150‑1 (MQ=255)
                                                                                                     gACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaac                                           <  1:828639/150‑1 (MQ=255)
                                                                                                                 ctcGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTAGCGCCATGAGCTAATAAAGACAAGTACGTTTACGTCACTATTTCAGTAAACAAACTATTGTCTTGCa                               >  1:1432803/1‑150 (MQ=255)
                                                                                                                   cGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCaaa                             <  2:1551214/150‑1 (MQ=255)
                                                                                                                        tGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGATTATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTCCAGTAAACAAACAATTGTCTGTCAAatcatc                        >  2:1131472/1‑150 (MQ=255)
                                                                                                                         gAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAAtcatca                       >  2:1643234/1‑150 (MQ=255)
                                                                                                                          aaCTAAGCGGGTGAATGCCGCCGATGGAGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGCCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCAc                      <  2:1272660/150‑1 (MQ=255)
                                                                                                                           aCTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCAcc                     <  2:1643127/150‑1 (MQ=255)
                                                                                                                           aCTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCAcc                     <  1:259982/150‑1 (MQ=255)
                                                                                                                                   tgtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCg             >  2:1350931/1‑150 (MQ=255)
                                                                                                                                   tgtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTAGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCg             >  1:1663269/1‑150 (MQ=255)
                                                                                                                                   aagcaaTGCCGCCATCGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCg             >  1:5967/5‑150 (MQ=255)
                                                                                                                                    gtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCCCTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGc            >  2:1509783/1‑150 (MQ=255)
                                                                                                                                    gtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTACACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGc            <  1:756535/150‑1 (MQ=255)
                                                                                                                                    agcaaTGCCGCCATCGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCCGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGc            >  1:1319530/4‑150 (MQ=255)
                                                                                                                                    agcaaTGCCGCCATCGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGc            >  1:1206859/4‑150 (MQ=255)
                                                                                                                                      gAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGCCAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCt          >  1:1276856/1‑150 (MQ=255)
                                                                                                                                         tGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTaa       >  2:647307/1‑150 (MQ=255)
                                                                                                                                              ccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGcc  <  1:565955/150‑1 (MQ=255)
                                                                                                                                              ccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGcc  <  1:1325865/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCC  >  NC_000913/1629291‑1629582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: