Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,642,370 C→G 10.7% intergenic (+303/+119) cspF → / ← quuQ Qin prophage; cold shock protein/Qin prophage; putative antitermination protein Q

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,642,3700CG10.7% 411.7 / 34.4 139intergenic (+303/+119)cspF/quuQQin prophage; cold shock protein/Qin prophage; putative antitermination protein Q
Reads supporting (aligned to +/- strand):  ref base C (54/70);  new base G (7/8);  total (61/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

Only 100 of 143 total aligned reads displayed.

TTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCGCT  >  NC_000913/1642224‑1642516
                                                                                                                                                  |                                                                                                                                                  
    gtTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCATCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGACCCATTTAAAGCACAAATCCCGCTCATCAGCg                                                                                                                                             <  2:1308137/150‑1 (MQ=255)
      ttAGGGTCATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAATCAAGTCACCAGCTAAATAATAAGTTAACACACTTGAGTCCCGGGATGAGCTTCAACATTACCATTGCCCCATTTAAAGCACTAACCCCGCTCATCAGCggg                                                                                                                                           <  1:860131/150‑1 (MQ=255)
      ttAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCggg                                                                                                                                           <  1:1273068/150‑1 (MQ=255)
       tAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGt                                                                                                                                          <  1:1359328/150‑1 (MQ=255)
         gggACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGttt                                                                                                                                        <  2:57713/150‑1 (MQ=255)
         gggACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGttt                                                                                                                                        <  2:1056617/150‑1 (MQ=255)
          ggACATAGTACTTGAAGAAAACATTTTAAACATCAGGCAAATAACCTATTCACCAGCTATATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGtttt                                                                                                                                       <  1:349129/150‑1 (MQ=255)
           gACATTGTACTGGAAGAAAACATTATAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACATACAAGAGTCCCAGGATGAGATTCAACATTACAATTGCCCAATTTAAAGCACAAAACCCGCTCATCAACGGGTATTc                                                                                                                                      >  1:223646/1‑150 (MQ=255)
            aCATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCt                                                                                                                                     <  1:1214945/150‑1 (MQ=255)
            aCATTGTACTGGAAGAAAAAATTTTAAACATAAGGCAAATAACCAAGTCACCTGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTTCCCCATTTAAAGCACAATACCCGCTCATCATCGGGTTTTCt                                                                                                                                     <  2:782674/150‑1 (MQ=255)
             cATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttgtg                                                                                                                                    <  1:784306/150‑4 (MQ=255)
                tGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACtt                                                                                                                                 <  2:1585743/150‑1 (MQ=255)
                tGTACTGGAAGAAAACATTTTAAACATCAGGCAAATACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACttt                                                                                                                                <  1:150715/150‑1 (MQ=255)
                  tACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACtttt                                                                                                                               >  2:1196826/1‑150 (MQ=255)
                    cTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGtttt                                                                                                                                       <  1:635234/140‑1 (MQ=255)
                    cTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTc                                                                                                                             <  2:1200490/150‑1 (MQ=255)
                    cTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTc                                                                                                                             >  1:58390/1‑150 (MQ=255)
                    cTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTc                                                                                                                             >  2:416419/1‑150 (MQ=255)
                    attgaagaaAACATTTTAAACATCAGGCATATAACCAAGTCACCATCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCTTCAGCGGGTTTTCTACTTTTTc                                                                                                                             <  1:83709/147‑1 (MQ=255)
                      ggAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCGACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCtt                                                                                                                           >  1:166325/1‑150 (MQ=255)
                      ggAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCtt                                                                                                                           >  2:1706834/1‑150 (MQ=255)
                       taagaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGTATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTa                                                                                                                          <  2:1413356/149‑1 (MQ=255)
                       gaagaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTa                                                                                                                          <  1:847413/150‑1 (MQ=255)
                       gaagaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTa                                                                                                                          <  1:714161/150‑1 (MQ=255)
                       gaagaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTa                                                                                                                          <  2:978666/150‑1 (MQ=255)
                         agaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTAAACAGACAAGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCAAAAGCGGGTTTTCTACTTTTTCTTAAc                                                                                                                        >  2:1361598/1‑150 (MQ=255)
                          gaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTATACTTTTTCTTAACg                                                                                                                       >  1:1340159/1‑150 (MQ=255)
                            aaaCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTc                                                                                                                     <  1:416419/150‑1 (MQ=255)
                             aaCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCg                                                                                                                    <  1:727023/150‑1 (MQ=255)
                             aaCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGAATCAACATTACCATTGCCCCATTTAAAGCACAAAACCCACTCATAAGAGGGTTTTATACTATTTCTTAACGTct                                                                                                                    >  2:912803/1‑149 (MQ=255)
                               cATTTTAAACATCAGGCAAATTACAAAGTCACCAGCTAAATAATAAGTTACCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCggg                                                                                                                  <  2:1452394/150‑1 (MQ=255)
                               cATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCggg                                                                                                                  <  2:1155452/150‑1 (MQ=255)
                               cATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCggg                                                                                                                  <  1:1140293/150‑1 (MQ=255)
                               aaTTTTACACATCACGCAAAAAACCAAGTCACCAGCTAAATAATCAGATAACAGACATGAGTCCCGGGAAGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCggg                                                                                                                  <  1:351023/149‑1 (MQ=255)
                                 ttttAAACATCAGGCAAATAACCAAGTCACCAGCGAAATAATAAGTTAACAGACATTAGTCCCGGGATTAGATTCAACATTACAAGTGCCCCATTTAAAGCACAAAACCCGATCATCAGCGGGTTTTCTACTGTTTATGAAAGTCggttt                                                                                                                >  2:257429/1‑147 (MQ=255)
                                  tttAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGtat                                                                                                               <  1:1196826/150‑1 (MQ=255)
                                   ttAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGtata                                                                                                              <  1:1610218/150‑1 (MQ=255)
                                    tAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATAc                                                                                                             <  2:789088/150‑1 (MQ=255)
                                     aaaCATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCAGGTATACa                                                                                                            >  2:105503/1‑150 (MQ=255)
                                      aaCATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCGACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACaa                                                                                                           <  2:166325/150‑1 (MQ=255)
                                        cATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAg                                                                                                         >  1:86471/1‑150 (MQ=255)
                                           cAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCtca                                                                                                                                                   >  1:1275506/1‑105 (MQ=255)
                                           cAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCtca                                                                                                                                                   <  2:1275506/105‑1 (MQ=255)
                                                   tAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGa                                                                                              <  1:328925/150‑1 (MQ=255)
                                                    aaCCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaa                                                                                             >  2:527017/1‑150 (MQ=255)
                                                     aCCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaa                                                                                            <  2:841456/150‑1 (MQ=255)
                                                           tCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAttt                                                                                      <  1:529111/150‑1 (MQ=255)
                                                           tCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAttt                                                                                      >  2:322634/1‑150 (MQ=255)
                                                            cACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAtttt                                                                                     <  2:10977/150‑1 (MQ=255)
                                                                   tAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGTGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCata                                                                              <  2:250102/150‑1 (MQ=255)
                                                                        aataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAttttt                                                                         <  2:1156259/150‑1 (MQ=255)
                                                                         ataaGTTAACAGACATGAGTCCCGGGTTGAGATTCAACTTTACCATTGCCCCATTTAAAGCACAAAACCCGATCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtttttt                                                                        <  1:1403126/150‑1 (MQ=255)
                                                                         ataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtttttt                                                                        <  1:1716389/150‑1 (MQ=255)
                                                                          taaGTTAACAGCCATGAGTCCCGGGTTGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAATCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTg                                                                       <  2:1041866/150‑1 (MQ=255)
                                                                           aaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGa                                                                      >  2:387094/1‑150 (MQ=255)
                                                                           aaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGa                                                                      >  1:1588762/1‑150 (MQ=255)
                                                                                aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAt                                                                 <  1:1166547/150‑1 (MQ=255)
                                                                                 aCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATg                                                                >  2:1387941/1‑150 (MQ=255)
                                                                                 aCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATg                                                                <  1:657154/150‑1 (MQ=255)
                                                                                  cAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGc                                                               >  1:1691011/1‑150 (MQ=255)
                                                                                  cAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGc                                                               <  1:194568/150‑1 (MQ=255)
                                                                                        tGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAATTTATCCATATTTTTTGAAAAATGCAAAcat                                                         >  1:1092689/1‑150 (MQ=255)
                                                                                           gTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatcat                                                      <  2:1588762/150‑1 (MQ=255)
                                                                                             cccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGt                                                    >  2:493046/1‑150 (MQ=255)
                                                                                             cccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGt                                                    >  1:551496/1‑150 (MQ=255)
                                                                                               cGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCg                                                  >  1:807446/1‑150 (MQ=255)
                                                                                                  gATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCa                                               <  2:58390/150‑1 (MQ=255)
                                                                                                  gATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCa                                               <  2:86471/150‑1 (MQ=255)
                                                                                                        aTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCa                                         >  1:1167863/1‑150 (MQ=255)
                                                                                                        aTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCa                                         >  1:874426/1‑150 (MQ=255)
                                                                                                         ttCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAg                                        <  2:1691011/150‑1 (MQ=255)
                                                                                                           cAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCa                                      >  1:939209/1‑150 (MQ=255)
                                                                                                           cAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCa                                      <  1:1596958/150‑1 (MQ=255)
                                                                                                             aCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCaaa                                    >  1:1438467/1‑150 (MQ=255)
                                                                                                                 tACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGATAAATGCAAAAATCATGTCGCCATCTTCAGCAAAAAta                                >  2:699569/1‑149 (MQ=255)
                                                                                                                   ccATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATAATGTCGCCATCTTCAGCAAAAATCAt                              >  2:808462/1‑150 (MQ=255)
                                                                                                                    cATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCAtt                             >  2:1001278/1‑150 (MQ=255)
                                                                                                                    cATTGCCACATTTAAAGCACCAAACCCGCTCATCAGAGGGGTTTCTACTTTTTCTTAACGTAGGTTATACAAAGCCAATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCACACATCATGTCGCCATCTTCAGCAAAAATCAtt                             <  1:257429/150‑1 (MQ=255)
                                                                                                                        gCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAATATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTAtc                         <  2:1340159/150‑1 (MQ=255)
                                                                                                                          cccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTAtctc                       <  1:527017/150‑1 (MQ=255)
                                                                                                                             aTTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCTGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTc                    <  1:105503/150‑1 (MQ=255)
                                                                                                                              tttAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCa                   <  2:799612/150‑1 (MQ=255)
                                                                                                                                  aagtagaaaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCtt               >  2:394778/7‑150 (MQ=255)
                                                                                                                                  aaGCACAAAACCCGCTCATCAGCGGGTTTTCTACTATTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCGAACTTCAGCAAAAATCATTTATCTCGTCAccgt               >  1:837980/1‑148 (MQ=255)
                                                                                                                                   agtagaaaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTc              >  1:1657846/6‑150 (MQ=255)
                                                                                                                                   agtagaaaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGGTGAAAATATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTAAGCAAAAATCATTTATCTCGTCACCTTc              >  1:682832/6‑150 (MQ=255)
                                                                                                                                   agtagaaaaCCCGCTGATGAGCGGGTTGTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATATCGTCACCTTc              >  1:873019/6‑150 (MQ=255)
                                                                                                                                    gCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTcc             >  2:1220689/1‑150 (MQ=255)
                                                                                                                                    gCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTcc             <  1:1621236/150‑1 (MQ=255)
                                                                                                                                    gCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCAGCGTTGAAAAAATTTTATCCATATTTTTTGAATAATGCAAACATAATGTCGCCATCTTGAGCAATAATCATTTTTATGGTAACCTTcc             >  1:1473519/1‑150 (MQ=255)
                                                                                                                                      acaAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTc           <  2:209249/150‑1 (MQ=255)
                                                                                                                                      acaAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTc           <  2:123387/150‑1 (MQ=255)
                                                                                                                                       gaaaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCGATCTTCAGCAAAAATCATTTATCTCGTCACCTTACTCa          >  1:1512675/2‑150 (MQ=255)
                                                                                                                                        aaaaCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTACCGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCAGGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCaa         >  1:825194/1‑150 (MQ=255)
                                                                                                                                         aaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAAt        >  1:27759/1‑150 (MQ=255)
                                                                                                                                         aaaCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAAt        <  1:496649/150‑1 (MQ=255)
                                                                                                                                           aCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTg      <  1:322634/150‑1 (MQ=255)
                                                                                                                                           aCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTg      <  2:1089601/150‑1 (MQ=255)
                                                                                                                                              cGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTc                                                   <  2:1235421/102‑1 (MQ=255)
                                                                                                                                              cGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTc                                                   >  1:1235421/1‑102 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCGCT  >  NC_000913/1642224‑1642516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: