Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,657,895 A→T 5.9% intergenic (+25/‑174) ynfD → / → ynfE DUF1161 family periplasmic protein/putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,657,8950AT 5.9% 333.3 / 6.5 115intergenic (+25/‑174)ynfD/ynfEDUF1161 family periplasmic protein/putative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (48/60);  new base T (4/3);  total (52/63)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTTATATA  >  NC_000913/1657748‑1658044
                                                                                                                                                   |                                                                                                                                                     
tCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCTCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATg                                                                                                                                                     <  1:1209858/150‑1 (MQ=255)
 cccAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGa                                                                                                                                                    >  2:791902/1‑150 (MQ=255)
  ccAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGAc                                                                                                                                                   >  2:1483210/1‑150 (MQ=255)
   cAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACa                                                                                                                                                  <  2:162658/150‑1 (MQ=255)
   cAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACa                                                                                                                                                  <  2:1392516/150‑1 (MQ=255)
   cAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACa                                                                                                                                                  <  1:1199728/150‑1 (MQ=255)
     ggTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACaaa                                                                                                                                                <  2:118886/150‑1 (MQ=255)
          tcgGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCgg                                                                                                                                           <  2:1695171/150‑1 (MQ=255)
            ggCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAGCGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCg                                                                                                                                         <  1:1088985/150‑1 (MQ=255)
            ggCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCg                                                                                                                                         <  2:903054/150‑1 (MQ=255)
            ggCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCg                                                                                                                                         <  1:1158806/150‑1 (MQ=255)
             gCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCgt                                                                                                                                        <  2:1324153/150‑2 (MQ=255)
                aTTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGAttt                                                                                                                                     <  2:382885/150‑1 (MQ=255)
                  tGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGa                                                                                                                                   >  1:1318224/1‑150 (MQ=255)
                  tGCGATAATGATACGCATAAAATTCTCTATACCCTCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGTACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGa                                                                                                                                   <  1:1304755/150‑1 (MQ=255)
                    cgcTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAAc                                                                                                                                 <  1:1087729/150‑1 (MQ=255)
                    cgcTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAAc                                                                                                                                 <  1:576126/150‑1 (MQ=255)
                     gcTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGAATTGACAAATCCGGCGATTTGAACa                                                                                                                                >  2:1714174/1‑150 (MQ=255)
                       tAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATa                                                                                                                              <  1:1326509/150‑1 (MQ=255)
                        aaTGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATac                                                                                                                             >  1:736804/1‑150 (MQ=255)
                               cGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATaa                                                                                                                      >  2:1096977/1‑150 (MQ=255)
                                 cATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATc                                                                                                                    <  2:1230557/150‑1 (MQ=255)
                                  aTAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGTTATGACAAATCCGGCGATTTGAACATACAACATAATcc                                                                                                                   <  2:812941/150‑1 (MQ=255)
                                  aTAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATatccc                                                                                                                   >  2:629312/1‑146 (MQ=255)
                                     aaaTTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCAc                                                                                                                >  2:1386853/1‑150 (MQ=255)
                                     aaaTTCTCTATACCCGAACCACCAGCTGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCAc                                                                                                                <  2:597150/150‑1 (MQ=255)
                                      aaTTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCAcc                                                                                                               <  2:77657/150‑1 (MQ=255)
                                         tctcTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACGACATAATCCCACCtta                                                                                                            >  1:644082/1‑150 (MQ=255)
                                            ctATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatta                                                                                                         >  1:1648558/1‑150 (MQ=255)
                                                 cccGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCAt                                                                                                    <  2:157727/150‑1 (MQ=255)
                                                    gCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATAcc                                                                                                 <  1:463230/150‑1 (MQ=255)
                                                         accaGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCt                                                                                            >  2:356581/1‑150 (MQ=255)
                                                         accaGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCt                                                                                            <  1:452768/150‑1 (MQ=255)
                                                           caGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAGCCGCAGTAACACATTATCGACTGAACGCCGGATATGACTAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATCCGCTTCTAt                                                                                          >  1:329098/1‑150 (MQ=255)
                                                           caGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTAt                                                                                          <  2:751527/150‑1 (MQ=255)
                                                              cGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGTAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGa                                                                                       >  1:1018211/1‑150 (MQ=255)
                                                               ggTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGat                                                                                      >  1:1270542/1‑150 (MQ=255)
                                                                 tAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGatat                                                                                    <  1:211222/150‑1 (MQ=255)
                                                                  aaCGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTAGTCATAACCTTCTATTGATATg                                                                                   >  2:1453203/1‑150 (MQ=255)
                                                                    cGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGa                                                                                 >  2:1674267/1‑150 (MQ=255)
                                                                      tctaTGCTCCCGCGCAGTCTAGCCAGGATGGCGAGCCTGACGAACCGCAGTAAAACATTATAGACTGAACGCCGTATATGACAAATCCCGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCAATTGATATGGAtt                                                                               <  1:97767/150‑1 (MQ=255)
                                                                        tctGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaa                                                                             <  2:755408/150‑1 (MQ=255)
                                                                          tGCTCCCGGGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCGGGCGATTTGAACATACAACATAATCCGAGCTTATTACTCATACCCTTCTATTGATTTGGATtaata                                                                           <  2:438445/150‑1 (MQ=255)
                                                                           gCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaataa                                                                          >  1:1207430/1‑150 (MQ=255)
                                                                            cTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTaataat                                                                         >  1:681952/1‑150 (MQ=255)
                                                                              cccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTc                                                                       >  1:739770/1‑150 (MQ=255)
                                                                              cccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTc                                                                       <  2:736804/150‑1 (MQ=255)
                                                                               ccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCt                                                                      <  1:1483210/150‑1 (MQ=255)
                                                                               ccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCt                                                                      <  2:327458/150‑1 (MQ=255)
                                                                                cgcgCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCtt                                                                     >  1:1553572/1‑150 (MQ=255)
                                                                                 gcgcAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTa                                                                    <  2:807270/150‑1 (MQ=255)
                                                                                    cAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAAcc                                                                 >  2:1335057/1‑150 (MQ=255)
                                                                                          aGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAAt                                                           >  2:1492601/1‑150 (MQ=255)
                                                                                          aGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAAt                                                           >  1:814547/1‑150 (MQ=255)
                                                                                           gCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATg                                                          >  1:303157/1‑150 (MQ=255)
                                                                                            ccAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATgg                                                         >  2:1633288/1‑150 (MQ=255)
                                                                                            ccAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATgg                                                         >  2:918072/1‑150 (MQ=255)
                                                                                             cAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATggg                                                        <  1:957956/150‑1 (MQ=255)
                                                                                               tgATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTa                                                      <  1:592062/149‑1 (MQ=255)
                                                                                               ggATGGCTCGCCTGCCGAACCGCAGTAACAACTTATCGAATGAACGCCGGATATGACAAATCGGGCGATTTGAACATACAACATACTCCCACCTTATTACTCATACCCTTCTATTGATATGGATAAATAATTCATAACCCAAAATGGGTa                                                      <  2:1170808/150‑1 (MQ=255)
                                                                                               ggATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTa                                                      <  1:791902/150‑1 (MQ=255)
                                                                                               ggATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTa                                                      <  2:1284159/150‑1 (MQ=255)
                                                                                               ggATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTa                                                      >  2:501973/1‑150 (MQ=255)
                                                                                                gATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAAACCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAg                                                     <  1:1267964/150‑1 (MQ=255)
                                                                                                    gcgcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTc                                                 <  1:1386853/150‑1 (MQ=255)
                                                                                                     cgcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTcc                                                >  1:1013090/1‑150 (MQ=255)
                                                                                                     cgcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTcc                                                >  2:473474/1‑150 (MQ=255)
                                                                                                        gcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCctc                                             <  2:1648558/150‑1 (MQ=255)
                                                                                                          cTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTa                                           <  2:1587218/150‑1 (MQ=255)
                                                                                                               ttaCCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGtt                                      <  1:108997/148‑1 (MQ=255)
                                                                                                                aaCCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTa                                     <  1:670718/150‑1 (MQ=255)
                                                                                                                 aCCGCAGTAACACATTATCGACTGAACGCCGTATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAg                                    <  2:1656855/150‑1 (MQ=255)
                                                                                                                 aCCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAg                                    <  2:739770/150‑1 (MQ=255)
                                                                                                                 aCCGCAGTAACACATTATCCACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCACACCTTATAACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCAAAAATGGGTAGACTCCATCTATTGTTat                                    >  2:311877/1‑149 (MQ=255)
                                                                                                                   cGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAgcg                                  <  1:701625/150‑1 (MQ=255)
                                                                                                                    gCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAgcgc                                 >  2:1523763/1‑150 (MQ=255)
                                                                                                                    gCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAgcgc                                 >  1:1699619/1‑150 (MQ=255)
                                                                                                                          acacATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTaaa                           <  1:1492601/150‑1 (MQ=255)
                                                                                                                             caTTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAatat                        >  1:904198/1‑150 (MQ=255)
                                                                                                                             caTTATCGACTGAACGCAGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAatat                        <  1:1415029/150‑1 (MQ=255)
                                                                                                                                 aTCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCaa                    <  1:1096977/150‑1 (MQ=255)
                                                                                                                                 aTCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCaa                    >  1:1345343/1‑150 (MQ=255)
                                                                                                                                  tCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAGTCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAat                   <  2:619510/150‑1 (MQ=255)
                                                                                                                                  tCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAat                   <  1:918072/150‑1 (MQ=255)
                                                                                                                                   cGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAata                  >  1:1704390/1‑150 (MQ=255)
                                                                                                                                   cGACTGAACGCCGGATATGACAAATCCGGAGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAata                  <  2:1259749/150‑1 (MQ=255)
                                                                                                                                   cGACTGAACGCCGGATATGACAAATAAGGCGATTTGAACATAAAAAATAATCAAACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAata                  >  2:146235/1‑150 (MQ=255)
                                                                                                                                     aCTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAatata                <  2:1013090/150‑1 (MQ=255)
                                                                                                                                     aCTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAatata                <  2:1291486/150‑1 (MQ=255)
                                                                                                                                     aCTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAAAATAAAatata                >  1:482287/1‑150 (MQ=255)
                                                                                                                                      cTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAttatat               >  2:149082/1‑150 (MQ=255)
                                                                                                                                       tGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATTGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAAtatata              >  1:253803/1‑150 (MQ=255)
                                                                                                                                       aaatcGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAAtatata              >  1:1294302/5‑150 (MQ=255)
                                                                                                                                        aatcGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCCCCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATaa             >  1:490911/4‑150 (MQ=255)
                                                                                                                                            gCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACtt         >  2:875196/1‑150 (MQ=255)
                                                                                                                                               tgATATTACAAATCCGGCGATTTGAACATACAACATAATACCACATTATTACTCATACCCTTCTTTTGATATGGATTAATAATGCTTAACCCAAAATGTGTAGACTCCCTCTATTGTTAGCGCGCTAAATAATCAATATATAAACTTTta      <  1:1516130/149‑1 (MQ=255)
                                                                                                                                               tgATATGACAAATCCTGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTAATGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTta      <  1:1124021/149‑1 (MQ=255)
                                                                                                                                               ggATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTta      <  2:469078/150‑1 (MQ=255)
                                                                                                                                               ggATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTta      <  2:1018211/150‑1 (MQ=255)
                                                                                                                                                   atGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCTCGCTAAATATTCAATATATAAACTTTtatata  >  1:200997/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTTATATA  >  NC_000913/1657748‑1658044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: