Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,442 C→G 6.8% L792V (CTG→GTG)  ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,4420CG 6.8% 382.2 / 12.6 116L792V (CTG→GTG) ynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (54/54);  new base G (3/5);  total (57/59)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 121 total aligned reads displayed.

AGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCT  >  NC_000913/1660294‑1660591
                                                                                                                                                    |                                                                                                                                                     
aGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACt                                                                                                                                                      >  2:237682/1‑150 (MQ=255)
  ttCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTACCCACTCTGCGCCCTTCACCACTgg                                                                                                                                                    >  1:1023061/1‑150 (MQ=255)
   tCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGc                                                                                                                                                   <  1:1566492/150‑1 (MQ=255)
         aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTgg                                                                                                                                                    <  1:560139/143‑1 (MQ=255)
         aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTgg                                                                                                                                                    >  2:560139/1‑143 (MQ=255)
          ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggc                                                                                                                                            <  1:703280/150‑2 (MQ=255)
          ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGa                                                                                                                                            >  1:216960/1‑150 (MQ=255)
           cAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggcg                                                                                                                                           <  1:1208790/150‑3 (MQ=255)
           cAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggcg                                                                                                                                           <  2:453864/150‑3 (MQ=255)
            aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggcgc                                                                                                                                          <  1:309254/150‑4 (MQ=255)
            aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAGGGGaaa                                                                                                                                          >  2:658584/1‑150 (MQ=255)
            aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                          <  1:1225204/150‑1 (MQ=255)
            aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                          >  2:809642/1‑150 (MQ=255)
             gCCAAAGTACCACCACGTATTCTCCCTGGAGTTTGCGCTATGGGCCAGGGAGCCTGGCACGCGGCCAATATGTCTGGCGATTTAATCGACCATGACGGCTGTGGGCATACGCTACCCACACTGCGCCCTTCCCCACTGGCGACGGGCAAc                                                                                                                                         >  2:1108239/1‑150 (MQ=255)
               ctaaGTAACTCCTCGTTTTCTCCCTGGAGTTAGCGCTATGTGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGTCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTTCGCCCTTCACCACTGGCGAAGGGAAAcca                                                                                                                                       <  1:19119/148‑2 (MQ=255)
               cAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGCCCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCCCTGGCGAAGGGaccccc                                                                                                                                       >  1:679873/1‑145 (MQ=255)
                aaaGTAACACCACGTATTCTCCCTGGAGTTAGCTCTATGTGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCTATAACCTCGACCATGGCGGCTGGGTGAATACGCTAACCACTCTGCGCCCTTCCCCAGTGGTGAAgggcgcagag                                                                                                                                      <  1:90772/150‑8 (MQ=255)
                 aaGTCACACCACGTATTCTCCCTGTTGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgc                                                                                                                                     <  2:856551/150‑1 (MQ=255)
                 aaGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgc                                                                                                                                     <  1:681861/150‑1 (MQ=255)
                 aaGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgc                                                                                                                                     <  2:509738/150‑1 (MQ=255)
                  aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCTATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgca                                                                                                                                    >  2:720677/1‑150 (MQ=255)
                   gTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgcag                                                                                                                                   <  2:641442/150‑1 (MQ=255)
                       caccccGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGCCCATGGCGGCTGTGTGAATACGCTACCCACTCGGCGCCCTTCACCCCTGGCGAAGGGCAACCCGCCGcaca                                                                                                                               >  1:262233/1‑150 (MQ=255)
                       caccacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGcaca                                                                                                                               >  2:1269465/1‑150 (MQ=255)
                        accacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCacac                                                                                                                              >  2:1574267/1‑150 (MQ=255)
                         cgtcGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATATAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACt                                                                                                                             <  2:242551/147‑1 (MQ=255)
                                 tctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGt                                                                                                                     >  1:1510033/1‑150 (MQ=255)
                                  ctcCCTGGAGTTCCCGCTCTGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc                                                                                                                    <  1:808923/150‑1 (MQ=255)
                                   tcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCTCGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCg                                                                                                                   <  1:685614/150‑1 (MQ=255)
                                   tcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCg                                                                                                                   <  2:1666279/150‑1 (MQ=255)
                                   tcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCAAAGGGAAACCCGCAGCACACTAATCTGGTCg                                                                                                                   >  1:1501605/1‑150 (MQ=255)
                                   acccTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCg                                                                                                                   <  1:1294423/149‑1 (MQ=255)
                                     ccTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgag                                                                                                                 <  2:1195172/150‑1 (MQ=255)
                                     ccTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgag                                                                                                                 <  2:216960/150‑1 (MQ=255)
                                      cTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgaga                                                                                                                >  2:1577804/1‑150 (MQ=255)
                                        ggAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATc                                                                                                              <  1:1640693/150‑1 (MQ=255)
                                          aGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGa                                                                                                            >  1:728913/1‑150 (MQ=255)
                                              aGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGTGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaa                                                                                                        >  2:1679754/1‑150 (MQ=255)
                                               gcgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa                                                                                                       <  1:111531/150‑1 (MQ=255)
                                                cgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAat                                                                                                      <  2:1510033/150‑1 (MQ=255)
                                                   tATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAAtata                                                                                                   <  1:638910/150‑1 (MQ=255)
                                                     tGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAc                                                                                                 <  1:1094146/150‑1 (MQ=255)
                                                        gCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCa                                                                                              >  2:777972/1‑150 (MQ=255)
                                                          cAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACg                                                                                            >  1:1388004/1‑150 (MQ=255)
                                                             ggAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGACCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACa                                                                                         <  1:252689/150‑1 (MQ=255)
                                                                gCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAcc                                                                                      <  1:493692/150‑1 (MQ=255)
                                                                 ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGCCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa                                                                                     >  1:1331332/1‑150 (MQ=255)
                                                                      cACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAAt                                                                                >  2:1402392/1‑150 (MQ=255)
                                                                        cGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTc                                                                              >  1:657176/1‑150 (MQ=255)
                                                                         gAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCt                                                                             <  2:1315077/150‑1 (MQ=255)
                                                                          aGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTg                                                                            <  1:501928/150‑1 (MQ=255)
                                                                                atatGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGa                                                                      <  2:1040743/150‑1 (MQ=255)
                                                                                 tatGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGAc                                                                     >  2:1587334/1‑150 (MQ=255)
                                                                                       tGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttg                                                               <  2:206373/150‑1 (MQ=255)
                                                                                         gCGATAAAATCGACCATGGCGGCTGTGTGAATACTCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACAAATCAGGTCTAAATCGAAAAAATATAACCCACGACAACCATAAATTCTGCCATGACATtttgat                                                             >  2:264709/1‑148 (MQ=255)
                                                                                         gCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt                                                             <  1:237682/150‑1 (MQ=255)
                                                                                         gCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt                                                             >  2:1064627/1‑150 (MQ=255)
                                                                                            aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGa                                                          >  2:1627469/1‑150 (MQ=255)
                                                                                              aaaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAGGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaa                                                        <  1:658584/150‑1 (MQ=255)
                                                                                              aaaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaa                                                        >  1:1499996/1‑150 (MQ=255)
                                                                                               aaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaaa                                                       <  2:1454350/150‑1 (MQ=255)
                                                                                                aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACACCCATAAATTCTGGCATGACATTTTGTTTGAAAAg                                                      >  1:763984/1‑150 (MQ=255)
                                                                                                 aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGc                                                     <  2:672047/150‑1 (MQ=255)
                                                                                                 aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGc                                                     <  1:1574267/150‑1 (MQ=255)
                                                                                                  tCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCa                                                    >  1:1219437/1‑150 (MQ=255)
                                                                                                     aCCATGGCTGCTGTGTGAATACGCTAACCACTCTGCGCCATTCACCACTGGCGAAGGTAAACCCGAAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTTGCATGACATTTTGTTTGAAAAGAAATa                                                 <  1:1480100/150‑1 (MQ=255)
                                                                                                     aCCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATa                                                 <  2:1219437/150‑1 (MQ=255)
                                                                                                     aCCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATa                                                 >  1:1133315/1‑150 (MQ=255)
                                                                                                     aCCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATa                                                 <  1:770124/150‑1 (MQ=255)
                                                                                                         tGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTg                                             >  2:1276673/1‑150 (MQ=255)
                                                                                                           gcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGACACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAg                                           >  2:1134634/1‑150 (MQ=255)
                                                                                                           gcggcTGTGTGAATACGCTAAACACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTACTCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAg                                           <  2:323357/150‑1 (MQ=255)
                                                                                                             ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTg                                             >  2:518493/1‑146 (MQ=255)
                                                                                                             ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTg                                             <  1:518493/146‑1 (MQ=255)
                                                                                                               cTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAat                                       <  1:1679754/150‑1 (MQ=255)
                                                                                                                tgtgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAatg                                      <  2:1331332/150‑1 (MQ=255)
                                                                                                                 gtgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAatga                                     >  1:521356/1‑150 (MQ=255)
                                                                                                                 gtgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGAGAAGGGAACCCCGCAGCACACTAATCAGGTCGAGATCGAAAAAAGATAACCCACGACAACCAAAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAatga                                     >  1:853999/1‑150 (MQ=255)
                                                                                                                          cGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATcc                            <  2:147845/150‑1 (MQ=255)
                                                                                                                          cGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATcc                            >  2:770261/1‑150 (MQ=255)
                                                                                                                              aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGaaaa                               <  2:1592508/143‑1 (MQ=255)
                                                                                                                              aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGaaaa                               >  1:1592508/1‑143 (MQ=255)
                                                                                                                              aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc                        <  1:1641024/150‑1 (MQ=255)
                                                                                                                                 cACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACcac                     <  2:724666/150‑1 (MQ=255)
                                                                                                                                   ctctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACag                   >  1:1141800/1‑150 (MQ=255)
                                                                                                                                    tctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACaga                  <  1:1519965/150‑1 (MQ=255)
                                                                                                                                      tGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAgg                <  1:1577804/150‑1 (MQ=255)
                                                                                                                                       tttcccTTCGCCAGTGGTGAAGGGAAACCAGCAGCACACTAATCTGGTCGAGCTCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgc               >  1:169891/4‑150 (MQ=255)
                                                                                                                                         gcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgcgc             <  1:570650/150‑1 (MQ=255)
                                                                                                                                          cccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCCGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCt            >  2:543918/1‑150 (MQ=255)
                                                                                                                                          cccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGtcgca            >  2:490095/1‑149 (MQ=255)
                                                                                                                                          cccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCt            >  2:1332638/1‑150 (MQ=255)
                                                                                                                                           ccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTc           >  1:89744/1‑150 (MQ=255)
                                                                                                                                            cTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCa          >  1:150493/1‑150 (MQ=255)
                                                                                                                                             ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCAt         <  1:1276673/150‑1 (MQ=255)
                                                                                                                                              tCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATg        <  2:1023061/150‑1 (MQ=255)
                                                                                                                                                 ccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAg     >  2:1189406/1‑150 (MQ=255)
                                                                                                                                                   acTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGc   <  1:1232449/150‑1 (MQ=255)
                                                                                                                                                    cTGGCGAAGGGAAACCCTCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCt  <  1:1178393/150‑1 (MQ=255)
                                                                                                                                                    cTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCt  <  2:1499996/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCT  >  NC_000913/1660294‑1660591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: