Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,673,540 T→G 6.2% intergenic (‑39/‑373) mdtJ ← / → tqsA multidrug efflux system transporter/pheromone AI‑2 transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,673,5400TG 6.2% 284.8 / 5.9 92intergenic (‑39/‑373)mdtJ/tqsAmultidrug efflux system transporter/pheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base T (44/42);  new base G (2/4);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATA  >  NC_000913/1673393‑1673687
                                                                                                                                                   |                                                                                                                                                   
ttAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTaa                                                                                                                                                   >  2:45130/1‑150 (MQ=255)
 tAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAAt                                                                                                                                                  <  2:930538/150‑1 (MQ=255)
  aaaaTAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAAtt                                                                                                                                                 <  1:249112/150‑1 (MQ=255)
   aaataaatCATCCATTTACCTCAATGACGATCGCCAATTTCATTTACAGCTTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCACCTGCAAGAGAATTATTTTAATTTTCGATTAATTc                                                                                                                                                >  2:792743/1‑150 (MQ=255)
     aTAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGAGTACCGGTAATTTCTGTATCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGAAAGAGAATTATTTTAATTTTCGCTTAATTCAg                                                                                                                                              <  2:1008371/150‑1 (MQ=255)
        aaGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGa                                                                                                                                           <  1:927951/150‑1 (MQ=255)
             gccATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCa                                                                                                                                      >  2:1379998/1‑150 (MQ=255)
             gccATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCa                                                                                                                                      <  1:438646/150‑1 (MQ=255)
              ccATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTGAATTAAGCGaaaatta                                                                                                                                     <  1:1218445/150‑5 (MQ=255)
               cATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaa                                                                                                                                    >  1:980973/1‑150 (MQ=255)
               cATTTCCCTCACTGACGCTCGCACATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTGAATTAAGCGaaaattaa                                                                                                                                    <  1:1136004/150‑6 (MQ=255)
                aTTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaag                                                                                                                                   <  1:569457/150‑1 (MQ=255)
                aTTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaag                                                                                                                                   <  1:918381/150‑1 (MQ=255)
                 tttCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTGAATTAAGCGaaaattaaaa                                                                                                                                  <  1:1415313/150‑8 (MQ=255)
                   tCCCTCACTGCCGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaa                                                                                                                                >  1:841880/1‑150 (MQ=255)
                   tCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaa                                                                                                                                >  2:1086600/1‑150 (MQ=255)
                     ccTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaaag                                                                                                                              <  1:45130/150‑1 (MQ=255)
                      cTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaaagt                                                                                                                             <  1:1217637/150‑1 (MQ=255)
                      cTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaaagt                                                                                                                             <  2:808936/150‑1 (MQ=255)
                       tCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTg                                                                                                                            >  1:663526/1‑150 (MQ=255)
                        cACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTgg                                                                                                                           <  2:1329838/150‑1 (MQ=255)
                            gACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCaa                                                                                                                       >  2:1521490/1‑150 (MQ=255)
                              cGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGc                                                                                                                     >  1:371090/1‑150 (MQ=255)
                               gCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGcc                                                                                                                    <  1:1086600/150‑1 (MQ=255)
                               gCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGcc                                                                                                                    <  2:456619/150‑1 (MQ=255)
                                    cccATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCcgcg                                                                                                               >  2:752100/1‑150 (MQ=255)
                                    cccATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCcgcg                                                                                                               >  2:948526/1‑150 (MQ=255)
                                    cccATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCcgcg                                                                                                               >  2:453618/1‑150 (MQ=255)
                                      cATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCa                                                                                                             <  2:897987/150‑1 (MQ=255)
                                           cATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAgcg                                                                                                        <  2:1455910/150‑1 (MQ=255)
                                           cATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAgcg                                                                                                        <  1:1569213/150‑1 (MQ=255)
                                            aTTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAgcgc                                                                                                       >  2:1402165/1‑150 (MQ=255)
                                              tGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCaa                                                                                                     >  1:1531352/1‑150 (MQ=255)
                                               gACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAg                                                                                                    <  2:1625989/150‑1 (MQ=255)
                                                     gTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAg                                                                                                          <  2:1560637/138‑1 (MQ=255)
                                                     gTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAg                                                                                                          >  1:1560637/1‑138 (MQ=255)
                                                      tACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGc                                                                                             <  1:517813/150‑1 (MQ=255)
                                                      tACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGc                                                                                             <  1:1667888/150‑1 (MQ=255)
                                                         cGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCaga                                                                                          >  2:162056/1‑150 (MQ=255)
                                                          ggTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCagaa                                                                                         >  1:1651104/1‑150 (MQ=255)
                                                          ggTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCagaa                                                                                         <  1:1521490/150‑1 (MQ=255)
                                                          ggTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGATTTGGCagaa                                                                                         >  2:363015/1‑150 (MQ=255)
                                                                ttCTGTAGCAATAGCCAGACATAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCTGAAGAAAgg                                                                                   <  2:1268027/150‑1 (MQ=255)
                                                                 tCTGTAGCAATAGCCAGACCTAATAAAATCCAATATATATTCATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGATACCAAAGAAATGTGGTCAAGCCCCGCGCTTAGCGCAAGCTTGGGCAGAAGAAAGGa                                                                                  >  2:80232/1‑150 (MQ=255)
                                                                 tCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGa                                                                                  >  2:714341/1‑150 (MQ=255)
                                                                   tGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACt                                                                                <  2:1531352/150‑1 (MQ=255)
                                                                    gTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTa                                                                               >  1:485711/1‑150 (MQ=255)
                                                                        cAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCt                                                                                                  <  2:778882/127‑1 (MQ=255)
                                                                        cAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCt                                                                                                  >  1:778882/1‑127 (MQ=255)
                                                                          aTAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAgcgc                                                                         >  1:969067/1‑150 (MQ=255)
                                                                             gCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGtt                                                                      >  1:471135/1‑150 (MQ=255)
                                                                             gCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGtt                                                                      >  1:960838/1‑150 (MQ=255)
                                                                              ccAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTg                                                                     <  1:656853/150‑1 (MQ=255)
                                                                                  aCCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGcc                                                                 <  2:841880/150‑1 (MQ=255)
                                                                                    cTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAg                                                               <  1:948526/150‑1 (MQ=255)
                                                                                     taataaAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGt                                                              <  2:1606232/150‑1 (MQ=255)
                                                                                      aataaAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGtg                                                             <  2:980973/150‑1 (MQ=255)
                                                                                      aataaAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGtg                                                             >  1:863591/1‑150 (MQ=255)
                                                                                       ataaAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGtgc                                                            <  1:497061/150‑1 (MQ=255)
                                                                                          aaaTCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGtgctgc                                                         >  1:545279/1‑150 (MQ=255)
                                                                                              ccAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCAc                                                     <  2:1094532/150‑1 (MQ=255)
                                                                                               cAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGGAAGCTTTGGCAGATGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCAcc                                                    >  2:267057/1‑150 (MQ=255)
                                                                                                aataaataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCt                                                   >  2:361413/1‑150 (MQ=255)
                                                                                                   aaataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACg                                                <  2:949328/150‑1 (MQ=255)
                                                                                                   aaataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACg                                                <  2:371090/150‑1 (MQ=255)
                                                                                                    aataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGa                                               >  1:1375223/1‑150 (MQ=255)
                                                                                                    aataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGa                                               >  1:655125/1‑150 (MQ=255)
                                                                                                         aCATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAg                                          >  2:289401/1‑150 (MQ=255)
                                                                                                         aCATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAg                                          >  2:953448/1‑150 (MQ=255)
                                                                                                         aCATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAg                                          >  2:1563740/1‑150 (MQ=255)
                                                                                                          ccttGTCCTTCTGCTGCAAGAGAATTATTTTAATTTTCGCTGAATTACGCGAAACCAAAGTAAAGTGGTCCAGCCCCGCGCATAGAGGAAGCTATGGCTGAAGAAAGGACTAACGCGCGTTGCGCCTGTGCTGCTCACCTGCGGGCAAgc                                         >  2:318489/3‑149 (MQ=255)
                                                                                                                ccTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTgg                                   <  1:453520/150‑1 (MQ=255)
                                                                                                                 cTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGa                                  <  2:545279/150‑1 (MQ=255)
                                                                                                                   tctcCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATg                                <  1:752100/150‑1 (MQ=255)
                                                                                                                   tctcCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATg                                >  1:1648950/1‑150 (MQ=255)
                                                                                                                      ccagCAAGAGATATCTTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAACGTGGTCACGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAACGACTAAAGCGCGTTGCGCCAGTGCTGCTCTCCTACGAGCAAGATAGTGGATGAgg                             <  1:80232/147‑1 (MQ=255)
                                                                                                                       cTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGt                            >  1:1327825/1‑150 (MQ=255)
                                                                                                                       cTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGt                            >  1:977528/1‑150 (MQ=255)
                                                                                                                        tGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTa                           <  2:445417/150‑1 (MQ=255)
                                                                                                                        tGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTa                           >  2:1563954/1‑150 (MQ=255)
                                                                                                                          caattgaATTATTTTAATTTTCGATTAATTCAGCGAAACCAATCTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGGTGCGACAGTGATGCTCACCTACGAGCAAGATAGTGGATGAGGTACg                         <  1:601652/145‑1 (MQ=255)
                                                                                                                                aaTTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATg                   <  2:655125/150‑1 (MQ=255)
                                                                                                                                aaTTATTTTAATTTTCGCTTAATACATCGAAACCAAACGAAAGTGGTCACGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCGGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATg                   <  2:1053834/150‑1 (MQ=255)
                                                                                                                                   tattTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGAAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGt                >  1:214243/1‑150 (MQ=255)
                                                                                                                                      tttAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTaaa             >  1:1483010/1‑150 (MQ=255)
                                                                                                                                       ttAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAAt            >  1:500541/1‑150 (MQ=255)
                                                                                                                                          aTTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGCGGTACGAAGATGGGTAAATGTa         >  1:90692/1‑150 (MQ=255)
                                                                                                                                           gtttCGCTGAATTAAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTa         <  1:1118893/148‑1 (MQ=255)
                                                                                                                                           gtttCGCTGAATTAAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTa         >  2:1118893/2‑149 (MQ=255)
                                                                                                                                              tCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGaaa     >  2:1389082/1‑150 (MQ=255)
                                                                                                                                                gCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAAt   >  2:1218832/1‑150 (MQ=255)
                                                                                                                                                 cTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATa  <  2:1651104/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATA  >  NC_000913/1673393‑1673687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: