Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,673,545 C→A 5.8% intergenic (‑44/‑368) mdtJ ← / → tqsA multidrug efflux system transporter/pheromone AI‑2 transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,673,5450CA 5.8% 284.0 / 4.2 90intergenic (‑44/‑368)mdtJ/tqsAmultidrug efflux system transporter/pheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base C (42/42);  new base A (2/4);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCC  >  NC_000913/1673396‑1673693
                                                                                                                                                     |                                                                                                                                                    
aaataaatCATCCATTTACCTCAATGACGATCGCCAATTTCATTTACAGCTTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCACCTGCAAGAGAATTATTTTAATTTTCGATTAATTc                                                                                                                                                      >  2:792743/1‑150 (MQ=255)
  aTAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGAGTACCGGTAATTTCTGTATCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGAAAGAGAATTATTTTAATTTTCGCTTAATTCAg                                                                                                                                                    <  2:1008371/150‑1 (MQ=255)
     aaGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGa                                                                                                                                                 <  1:927951/150‑1 (MQ=255)
          gccATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCa                                                                                                                                            <  1:438646/150‑1 (MQ=255)
          gccATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCa                                                                                                                                            >  2:1379998/1‑150 (MQ=255)
           ccATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTGAATTAAGCGaaaatta                                                                                                                                           <  1:1218445/150‑5 (MQ=255)
            cATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaa                                                                                                                                          >  1:980973/1‑150 (MQ=255)
            cATTTCCCTCACTGACGCTCGCACATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTGAATTAAGCGaaaattaa                                                                                                                                          <  1:1136004/150‑6 (MQ=255)
             aTTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaag                                                                                                                                         <  1:918381/150‑1 (MQ=255)
             aTTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaag                                                                                                                                         <  1:569457/150‑1 (MQ=255)
              tttCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTGAATTAAGCGaaaattaaaa                                                                                                                                        <  1:1415313/150‑8 (MQ=255)
                tCCCTCACTGCCGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaa                                                                                                                                      >  1:841880/1‑150 (MQ=255)
                tCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaa                                                                                                                                      >  2:1086600/1‑150 (MQ=255)
                  ccTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaaag                                                                                                                                    <  1:45130/150‑1 (MQ=255)
                   cTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaaagt                                                                                                                                   <  1:1217637/150‑1 (MQ=255)
                   cTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCaaagtaaagt                                                                                                                                   <  2:808936/150‑1 (MQ=255)
                    tCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTg                                                                                                                                  >  1:663526/1‑150 (MQ=255)
                     cACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTgg                                                                                                                                 <  2:1329838/150‑1 (MQ=255)
                         gACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCaa                                                                                                                             >  2:1521490/1‑150 (MQ=255)
                           cGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGc                                                                                                                           >  1:371090/1‑150 (MQ=255)
                            gCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGcc                                                                                                                          <  1:1086600/150‑1 (MQ=255)
                            gCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGcc                                                                                                                          <  2:456619/150‑1 (MQ=255)
                                 cccATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCcgcg                                                                                                                     >  2:453618/1‑150 (MQ=255)
                                 cccATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCcgcg                                                                                                                     >  2:948526/1‑150 (MQ=255)
                                 cccATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCcgcg                                                                                                                     >  2:752100/1‑150 (MQ=255)
                                   cATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCa                                                                                                                   <  2:897987/150‑1 (MQ=255)
                                        cATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAgcg                                                                                                              <  2:1455910/150‑1 (MQ=255)
                                        cATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAgcg                                                                                                              <  1:1569213/150‑1 (MQ=255)
                                         aTTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAgcgc                                                                                                             >  2:1402165/1‑150 (MQ=255)
                                           tGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCaa                                                                                                           >  1:1531352/1‑150 (MQ=255)
                                            gACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAg                                                                                                          <  2:1625989/150‑1 (MQ=255)
                                                  gTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAg                                                                                                                <  2:1560637/138‑1 (MQ=255)
                                                  gTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAg                                                                                                                >  1:1560637/1‑138 (MQ=255)
                                                   tACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGc                                                                                                   <  1:517813/150‑1 (MQ=255)
                                                   tACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGc                                                                                                   <  1:1667888/150‑1 (MQ=255)
                                                      cGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCaga                                                                                                >  2:162056/1‑150 (MQ=255)
                                                       ggTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCagaa                                                                                               <  1:1521490/150‑1 (MQ=255)
                                                       ggTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCagaa                                                                                               >  1:1651104/1‑150 (MQ=255)
                                                       ggTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGATTTGGCagaa                                                                                               >  2:363015/1‑150 (MQ=255)
                                                             ttCTGTAGCAATAGCCAGACATAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCTGAAGAAAgg                                                                                         <  2:1268027/150‑1 (MQ=255)
                                                              tCTGTAGCAATAGCCAGACCTAATAAAATCCAATATATATTCATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGATACCAAAGAAATGTGGTCAAGCCCCGCGCTTAGCGCAAGCTTGGGCAGAAGAAAGGa                                                                                        >  2:80232/1‑150 (MQ=255)
                                                              tCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGa                                                                                        >  2:714341/1‑150 (MQ=255)
                                                                tGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACt                                                                                      <  2:1531352/150‑1 (MQ=255)
                                                                 gTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTa                                                                                     >  1:485711/1‑150 (MQ=255)
                                                                     cAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCt                                                                                                        >  1:778882/1‑127 (MQ=255)
                                                                     cAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCt                                                                                                        <  2:778882/127‑1 (MQ=255)
                                                                       aTAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAgcgc                                                                               >  1:969067/1‑150 (MQ=255)
                                                                          gCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGtt                                                                            >  1:960838/1‑150 (MQ=255)
                                                                          gCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGtt                                                                            >  1:471135/1‑150 (MQ=255)
                                                                           ccAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTg                                                                           <  1:656853/150‑1 (MQ=255)
                                                                               aCCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGcc                                                                       <  2:841880/150‑1 (MQ=255)
                                                                                 cTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAg                                                                     <  1:948526/150‑1 (MQ=255)
                                                                                  taataaAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGt                                                                    <  2:1606232/150‑1 (MQ=255)
                                                                                   aataaAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGtg                                                                   <  2:980973/150‑1 (MQ=255)
                                                                                   aataaAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGtg                                                                   >  1:863591/1‑150 (MQ=255)
                                                                                    ataaAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGtgc                                                                  <  1:497061/150‑1 (MQ=255)
                                                                                       aaaTCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGtgctgc                                                               >  1:545279/1‑150 (MQ=255)
                                                                                           ccAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCAc                                                           <  2:1094532/150‑1 (MQ=255)
                                                                                            cAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGGAAGCTTTGGCAGATGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCAcc                                                          >  2:267057/1‑150 (MQ=255)
                                                                                             aataaataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCt                                                         >  2:361413/1‑150 (MQ=255)
                                                                                                aaataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACg                                                      <  2:949328/150‑1 (MQ=255)
                                                                                                aaataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACg                                                      <  2:371090/150‑1 (MQ=255)
                                                                                                 aataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGa                                                     >  1:1375223/1‑150 (MQ=255)
                                                                                                 aataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGa                                                     >  1:655125/1‑150 (MQ=255)
                                                                                                      aCATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAg                                                >  2:953448/1‑150 (MQ=255)
                                                                                                      aCATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAg                                                >  2:289401/1‑150 (MQ=255)
                                                                                                      aCATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAg                                                >  2:1563740/1‑150 (MQ=255)
                                                                                                       ccttGTCCTTCTGCTGCAAGAGAATTATTTTAATTTTCGCTGAATTACGCGAAACCAAAGTAAAGTGGTCCAGCCCCGCGCATAGAGGAAGCTATGGCTGAAGAAAGGACTAACGCGCGTTGCGCCTGTGCTGCTCACCTGCGGGCAAgc                                               >  2:318489/3‑149 (MQ=255)
                                                                                                             ccTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTgg                                         <  1:453520/150‑1 (MQ=255)
                                                                                                              cTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGa                                        <  2:545279/150‑1 (MQ=255)
                                                                                                                tctcCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATg                                      >  1:1648950/1‑150 (MQ=255)
                                                                                                                tctcCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATg                                      <  1:752100/150‑1 (MQ=255)
                                                                                                                   ccagCAAGAGATATCTTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAACGTGGTCACGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAACGACTAAAGCGCGTTGCGCCAGTGCTGCTCTCCTACGAGCAAGATAGTGGATGAgg                                   <  1:80232/147‑1 (MQ=255)
                                                                                                                    cTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGt                                  >  1:977528/1‑150 (MQ=255)
                                                                                                                    cTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGt                                  >  1:1327825/1‑150 (MQ=255)
                                                                                                                     tGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTa                                 >  2:1563954/1‑150 (MQ=255)
                                                                                                                     tGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTa                                 <  2:445417/150‑1 (MQ=255)
                                                                                                                       caattgaATTATTTTAATTTTCGATTAATTCAGCGAAACCAATCTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGGTGCGACAGTGATGCTCACCTACGAGCAAGATAGTGGATGAGGTACg                               <  1:601652/145‑1 (MQ=255)
                                                                                                                             aaTTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATg                         <  2:655125/150‑1 (MQ=255)
                                                                                                                             aaTTATTTTAATTTTCGCTTAATACATCGAAACCAAACGAAAGTGGTCACGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCGGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATg                         <  2:1053834/150‑1 (MQ=255)
                                                                                                                                tattTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGAAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGt                      >  1:214243/1‑150 (MQ=255)
                                                                                                                                   tttAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTaaa                   >  1:1483010/1‑150 (MQ=255)
                                                                                                                                    ttAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAAt                  >  1:500541/1‑150 (MQ=255)
                                                                                                                                       aTTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGCGGTACGAAGATGGGTAAATGTa               >  1:90692/1‑150 (MQ=255)
                                                                                                                                        gtttCGCTGAATTAAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTa               <  1:1118893/148‑1 (MQ=255)
                                                                                                                                        gtttCGCTGAATTAAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTa               >  2:1118893/2‑149 (MQ=255)
                                                                                                                                           tCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGaaa           >  2:1389082/1‑150 (MQ=255)
                                                                                                                                             gCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAAt         >  2:1218832/1‑150 (MQ=255)
                                                                                                                                              cTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATa        <  2:1651104/150‑1 (MQ=255)
                                                                                                                                                 aaTTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACg     <  2:1375223/150‑1 (MQ=255)
                                                                                                                                                    tCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTcc  <  2:1265575/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCAGACCTAATAAAATCCAATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCC  >  NC_000913/1673396‑1673693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: