Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,724,616 C→T 6.0% A14T (GCA→ACA)  sodC ← superoxide dismutase, Cu, Zn, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,724,6160CT 6.0% 318.8 / 5.3 102A14T (GCA→ACA) sodCsuperoxide dismutase, Cu, Zn, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (51/44);  new base T (4/2);  total (56/46)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01

Only 100 of 104 total aligned reads displayed.

TTCACCGGGGGGTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTGCCG  >  NC_000913/1724467‑1724761
                                                                                                                                                     |                                                                                                                                                 
ttCACCGGGGGGTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCACGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGc                                                                                                                                                   >  1:1451068/1‑150 (MQ=255)
 tCACCGGGGGGTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGca                                                                                                                                                  >  2:1609850/1‑150 (MQ=255)
       gggggTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAAcc                                                                                                                                            <  2:1358924/150‑1 (MQ=255)
           gTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAgcg                                                                                                                                        >  2:943509/1‑150 (MQ=255)
           gTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAgcg                                                                                                                                        <  2:398872/150‑1 (MQ=255)
           gTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAgcg                                                                                                                                        >  2:1533333/1‑150 (MQ=255)
               tGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCTGGTTGTTGCAACCGgcgcaca                                                                                                                                    <  2:748933/150‑4 (MQ=255)
               tGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAg                                                                                                                                    <  2:1487188/150‑1 (MQ=255)
                gCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGa                                                                                                                                   >  1:1048056/1‑150 (MQ=255)
                gCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGa                                                                                                                                   >  1:368820/1‑150 (MQ=255)
                  tttCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCTGGTTGTTGCAACCGgcgcacaagc                                                                                                                                 <  2:358343/150‑7 (MQ=255)
                   ttCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCACTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACAAGCGCCAGAATa                                                                                                                                >  1:1399480/1‑150 (MQ=255)
                     cataTCGCGCGAACAATCCAGACCTTTATCGCTTTCAGTAATGTTGACGCGACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGc                                                                                                                              <  1:302507/147‑1 (MQ=255)
                     cAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGc                                                                                                                              >  1:1319871/1‑150 (MQ=255)
                      aGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGATTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGcc                                                                                                                             >  1:1065077/1‑150 (MQ=255)
                        aTCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAg                                                                                                                           >  1:1435447/1‑150 (MQ=255)
                          cGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGAc                                                                                                                         <  2:1624910/150‑1 (MQ=255)
                            ggCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTa                                                                                                                       >  2:1378323/1‑150 (MQ=255)
                             gCGAAAACTCCAGACCTTTATCGGTTTCAGTAATTGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTaa                                                                                                                      <  1:22469/150‑1 (MQ=255)
                             gCGAAAACTCCAGACCTTTATCGGTTGCAGTTATGCTGACGCTACCAATTGACTCCCCTACCCCTTGCGACGTGACGAGGATCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTaa                                                                                                                      <  1:1152427/150‑1 (MQ=255)
                               gAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTaaaa                                                                                                                    >  2:861347/1‑150 (MQ=255)
                                aaaaCTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTTCGCCGGTTGCAACAACCCGCGCCAGAATAGCCAGACTAAAAc                                                                                                                   >  1:302218/1‑150 (MQ=255)
                                aaaaCTCCAGACCTTATTCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTGGCAACAACCAGCGCCAGAATAGCCAGACTAAAAc                                                                                                                   <  2:1085114/150‑1 (MQ=255)
                                 aaaCTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACg                                                                                                                  <  2:1653466/150‑1 (MQ=255)
                                     tCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTc                                                                                                              <  2:32824/150‑1 (MQ=255)
                                     tCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTc                                                                                                              <  1:1622422/150‑1 (MQ=255)
                                      ccAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCa                                                                                                             >  2:1609766/1‑150 (MQ=255)
                                        aGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATa                                                                                                           <  1:1150007/150‑1 (MQ=255)
                                          aCCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAgg                                                                                                         <  2:546319/150‑1 (MQ=255)
                                           ccTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGa                                                                                                        <  1:1718581/150‑1 (MQ=255)
                                           ccTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCACTTGAGACGTGACGAGGTTCATCTAGAATTTTTCACTTGCAGATTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGa                                                                                                        >  1:699438/1‑150 (MQ=255)
                                              ttATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGAGACGAGAAGAGGTTCATCTCGAATTTTTAACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCt                                                                                                     >  1:1638392/1‑150 (MQ=255)
                                                   ggTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGtt                                                                                                >  2:1354117/1‑150 (MQ=255)
                                                   ggTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGtt                                                                                                >  2:1548227/1‑150 (MQ=255)
                                                   ggTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGAGCCGGTTTCAACAACCAGCTCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCAGtt                                                                                                >  1:995763/1‑150 (MQ=255)
                                                       tCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCAtt                                                                                            >  2:312244/1‑150 (MQ=255)
                                                        cAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtg                                                                                           <  1:1533333/150‑1 (MQ=255)
                                                        cAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtg                                                                                           <  2:1513138/150‑1 (MQ=255)
                                                        cAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtg                                                                                           <  2:1319871/150‑1 (MQ=255)
                                                        cAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtg                                                                                           >  1:1259455/1‑150 (MQ=255)
                                                        cAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtg                                                                                           <  1:943509/150‑1 (MQ=255)
                                                          gTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtgtg                                                                                         >  2:1356720/1‑150 (MQ=255)
                                                           tAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGTCAGCTTGTGCGCCGGTTGCAACTACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGc                                                                                        <  1:1064974/150‑1 (MQ=255)
                                                             aTGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCAt                                                                                      <  1:1428659/150‑1 (MQ=255)
                                                              tGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGATTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCAtt                                                                                     <  2:1065077/150‑1 (MQ=255)
                                                                     gCTCCCAATTGACTTCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCGTGTGCGCCGGTTGCAACTACCAGCGCCATAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAAt                                                                              <  2:925875/150‑1 (MQ=255)
                                                                     gCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAAt                                                                              <  2:1002717/150‑1 (MQ=255)
                                                                       tACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATaa                                                                            >  2:191440/1‑150 (MQ=255)
                                                                          cAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAgtg                                                                         <  1:1646322/150‑1 (MQ=255)
                                                                             ttGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTaa                                                                      <  2:1451068/150‑1 (MQ=255)
                                                                               gACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACCACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAAc                                                                    >  2:227919/1‑150 (MQ=255)
                                                                               gACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAAc                                                                    <  1:861347/150‑1 (MQ=255)
                                                                               gACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAAc                                                                    >  1:374294/1‑150 (MQ=255)
                                                                                  tGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAg                                                                 <  1:1378323/150‑1 (MQ=255)
                                                                                  tGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAg                                                                 >  2:308459/1‑150 (MQ=255)
                                                                                       tACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGaca                                                            <  1:312244/150‑1 (MQ=255)
                                                                                           ccTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACAtt                                                        >  2:1344996/1‑150 (MQ=255)
                                                                                             ttGCGATGTGTCGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACAtttt                                                      >  1:237748/1‑150 (MQ=255)
                                                                                             ttGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACAtttt                                                      <  2:249210/150‑1 (MQ=255)
                                                                                              tGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACAttttt                                                     >  2:1551000/1‑150 (MQ=255)
                                                                                               tcGACGTGACGAGGGTCATCTCGACTGTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTg                                                    <  2:72118/149‑1 (MQ=255)
                                                                                                 gACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGaa                                                  >  2:414910/1‑150 (MQ=255)
                                                                                                 gACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGaa                                                  >  1:194204/1‑150 (MQ=255)
                                                                                                    gTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAAcgg                                               >  1:1488852/1‑150 (MQ=255)
                                                                                                      gACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAAcggcg                                             >  1:29251/1‑150 (MQ=255)
                                                                                                        cGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGGAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTACGTTCATAGTGCATACCTGAATAAGAGTAAACCAGTGACAAATTTTTGAAAggctgc                                           >  1:1113552/1‑150 (MQ=255)
                                                                                                           ggTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTAt                                        >  1:1478155/1‑150 (MQ=255)
                                                                                                             ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTc                                      >  1:1223548/1‑150 (MQ=255)
                                                                                                                aTCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAATGGCGGCTATTCCTa                                   <  1:969365/150‑1 (MQ=255)
                                                                                                                 tctcGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAACCGTTTCATAGGACCTCCGTACATTGTGCATTCCTGAATAAGTGTAACCCAGTGACACATTTTTGAACGGCGGCTATTCCTaa                                  >  1:1027006/1‑150 (MQ=255)
                                                                                                                  ctcGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTaaa                                 >  1:299322/1‑150 (MQ=255)
                                                                                                                  ctcGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCAAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTaaa                                 >  1:264705/1‑150 (MQ=255)
                                                                                                                      aCTTTTTCTCTGGCAGCTTGATCGCAGGTTGCAACAACCAGCGACAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTTACACATTTTTGAACGGCGGCTATTCCTAAAAGTg                             <  2:88658/150‑1 (MQ=255)
                                                                                                                      aCTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTg                             <  1:166666/150‑1 (MQ=255)
                                                                                                                      aCTTTTTCACTGGCAGCTCGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTg                             <  1:534715/150‑1 (MQ=255)
                                                                                                                       cTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGc                            >  1:1190129/1‑150 (MQ=255)
                                                                                                                       cTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGc                            >  1:1346001/1‑150 (MQ=255)
                                                                                                                         ttttCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCtt                          <  2:368820/150‑1 (MQ=255)
                                                                                                                           ttCACTGGCAGCTTGTGCGCCGGTTGCAACCACCAGCGCCAGAATAGCCAAACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTAc                        >  2:816489/1‑150 (MQ=255)
                                                                                                                           ttCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTAc                        <  1:1609766/150‑1 (MQ=255)
                                                                                                                             cACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTTAACGGCGGCTATTCCTAAAAGTGCTTACgg                      <  2:1399480/150‑1 (MQ=255)
                                                                                                                             cACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTTAAAGTGCTTACgg                      >  2:62783/1‑150 (MQ=255)
                                                                                                                               cTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTa                    >  1:1198463/1‑150 (MQ=255)
                                                                                                                               cTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTa                    >  2:670185/1‑150 (MQ=255)
                                                                                                                                 ggCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTAcg                  >  1:1588979/1‑150 (MQ=255)
                                                                                                                                 ggCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTAcg                  <  2:1618762/150‑1 (MQ=255)
                                                                                                                                     gCTTGTGCGCCGGTTGCCACAACCAGCGCCAGAATAGCCAGACTAAACCGTTTCATAGGACCTGCGTTCATTGTGCTTTCCTGCATAAGTGTAAACCAGTGCCCCCGTTTTGAACGGCGGCTATTCCTAAAAGTTCTTACGGTaagaagt              >  2:814179/1‑144 (MQ=255)
                                                                                                                                        tgtgCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAct           <  1:308459/150‑2 (MQ=255)
                                                                                                                                         tggcgcTGGTTGTTGCAACCGGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTTCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAccc          >  1:980834/3‑150 (MQ=255)
                                                                                                                                         gtgCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAccc          >  1:1361822/1‑150 (MQ=255)
                                                                                                                                         gtgCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAccc          <  2:932714/150‑1 (MQ=255)
                                                                                                                                          tgCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAcccc         >  1:46679/1‑150 (MQ=255)
                                                                                                                                          ggcgcTGGTTGTTGCAACCGGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAcccc         >  1:752621/2‑150 (MQ=255)
                                                                                                                                          ggcgcTGGTTGTTGCAACCGGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAcccc         >  2:1016319/2‑150 (MQ=255)
                                                                                                                                           gcgcTGGTTGTTGCAACCGGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCa        >  2:498795/1‑150 (MQ=255)
                                                                                                                                           gcgaCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTACGTTCATTGTGCATTCCTGAATAAATGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACACCa        >  1:178648/1‑150 (MQ=255)
                                                                                                                                            cgcCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAg       >  2:49944/1‑150 (MQ=255)
                                                                                                                                              ccGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTg     <  1:1609850/150‑1 (MQ=255)
                                                                                                                                               cGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTGc    <  1:473202/150‑1 (MQ=255)
                                                                                                                                                 gTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTGCCg  <  2:1259455/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTCACCGGGGGGTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTGCCG  >  NC_000913/1724467‑1724761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: