Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,724,618 A→G 5.9% V13A (GTT→GCT) ‡ sodC ← superoxide dismutase, Cu, Zn, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,724,6180AG 5.9% 297.4 / 7.3 102V13A (GTT→GCT) ‡sodCsuperoxide dismutase, Cu, Zn, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (52/44);  new base G (4/2);  total (56/46)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 103 total aligned reads displayed.

GGGGGTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTGCCGCTTTA  >  NC_000913/1724474‑1724766
                                                                                                                                                |                                                                                                                                                    
gggggTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAAcc                                                                                                                                                 <  2:1358924/150‑1 (MQ=255)
    gTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAgcg                                                                                                                                             >  2:943509/1‑150 (MQ=255)
    gTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAgcg                                                                                                                                             <  2:398872/150‑1 (MQ=255)
    gTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAgcg                                                                                                                                             >  2:1533333/1‑150 (MQ=255)
        tGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCTGGTTGTTGCAACCGgcgcaca                                                                                                                                         <  2:748933/150‑4 (MQ=255)
        tGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAg                                                                                                                                         <  2:1487188/150‑1 (MQ=255)
         gCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGa                                                                                                                                        >  1:1048056/1‑150 (MQ=255)
         gCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGa                                                                                                                                        >  1:368820/1‑150 (MQ=255)
           tttCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCTGGTTGTTGCAACCGgcgcacaagc                                                                                                                                      <  2:358343/150‑7 (MQ=255)
            ttCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCACTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACAAGCGCCAGAATa                                                                                                                                     >  1:1399480/1‑150 (MQ=255)
              cataTCGCGCGAACAATCCAGACCTTTATCGCTTTCAGTAATGTTGACGCGACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGc                                                                                                                                   <  1:302507/147‑1 (MQ=255)
              cAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGc                                                                                                                                   >  1:1319871/1‑150 (MQ=255)
               aGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGATTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGcc                                                                                                                                  >  1:1065077/1‑150 (MQ=255)
                 aTCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAg                                                                                                                                >  1:1435447/1‑150 (MQ=255)
                   cGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGAc                                                                                                                              <  2:1624910/150‑1 (MQ=255)
                     ggCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTa                                                                                                                            >  2:1378323/1‑150 (MQ=255)
                     ggCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTa                                                                                                                            >  2:1646322/1‑150 (MQ=255)
                      gCGAAAACTCCAGACCTTTATCGGTTTCAGTAATTGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTaa                                                                                                                           <  1:22469/150‑1 (MQ=255)
                      gCGAAAACTCCAGACCTTTATCGGTTGCAGTTATGCTGACGCTACCAATTGACTCCCCTACCCCTTGCGACGTGACGAGGATCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTaa                                                                                                                           <  1:1152427/150‑1 (MQ=255)
                        gAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTaaaa                                                                                                                         >  2:861347/1‑150 (MQ=255)
                         aaaaCTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTTCGCCGGTTGCAACAACCCGCGCCAGAATAGCCAGACTAAAAc                                                                                                                        >  1:302218/1‑150 (MQ=255)
                         aaaaCTCCAGACCTTATTCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTGGCAACAACCAGCGCCAGAATAGCCAGACTAAAAc                                                                                                                        <  2:1085114/150‑1 (MQ=255)
                          aaaCTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACg                                                                                                                       >  1:1618762/1‑150 (MQ=255)
                          aaaCTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACg                                                                                                                       <  2:1653466/150‑1 (MQ=255)
                              tCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTc                                                                                                                   <  1:1622422/150‑1 (MQ=255)
                              tCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTc                                                                                                                   <  2:32824/150‑1 (MQ=255)
                               ccAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCa                                                                                                                  >  2:1609766/1‑150 (MQ=255)
                                 aGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATa                                                                                                                <  1:1150007/150‑1 (MQ=255)
                                   aCCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAgg                                                                                                              <  2:546319/150‑1 (MQ=255)
                                    ccTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGa                                                                                                             <  1:1718581/150‑1 (MQ=255)
                                    ccTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCACTTGAGACGTGACGAGGTTCATCTAGAATTTTTCACTTGCAGATTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGa                                                                                                             >  1:699438/1‑150 (MQ=255)
                                       ttATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGAGACGAGAAGAGGTTCATCTCGAATTTTTAACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCt                                                                                                          >  1:1638392/1‑150 (MQ=255)
                                            ggTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGtt                                                                                                     >  2:1548227/1‑150 (MQ=255)
                                            ggTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGtt                                                                                                     >  2:1354117/1‑150 (MQ=255)
                                            ggTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGAGCCGGTTTCAACAACCAGCTCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCAGtt                                                                                                     >  1:995763/1‑150 (MQ=255)
                                                tCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCAtt                                                                                                 >  2:312244/1‑150 (MQ=255)
                                                 cAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtg                                                                                                <  1:943509/150‑1 (MQ=255)
                                                 cAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtg                                                                                                <  1:1533333/150‑1 (MQ=255)
                                                 cAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtg                                                                                                <  2:1319871/150‑1 (MQ=255)
                                                 cAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtg                                                                                                >  1:1259455/1‑150 (MQ=255)
                                                   gTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATtgtg                                                                                              >  2:1356720/1‑150 (MQ=255)
                                                    tAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGTCAGCTTGTGCGCCGGTTGCAACTACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGc                                                                                             <  1:1064974/150‑1 (MQ=255)
                                                      aTGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCAt                                                                                           <  1:1428659/150‑1 (MQ=255)
                                                       tGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGATTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCAtt                                                                                          <  2:1065077/150‑1 (MQ=255)
                                                              gCTCCCAATTGACTTCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCGTGTGCGCCGGTTGCAACTACCAGCGCCATAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAAt                                                                                   <  2:925875/150‑1 (MQ=255)
                                                              gCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAAt                                                                                   <  2:1002717/150‑1 (MQ=255)
                                                                tACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATaa                                                                                 >  2:191440/1‑150 (MQ=255)
                                                                   cAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAgtg                                                                              <  1:1646322/150‑1 (MQ=255)
                                                                      ttGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTaa                                                                           <  2:1451068/150‑1 (MQ=255)
                                                                        gACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACCACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAAc                                                                         >  2:227919/1‑150 (MQ=255)
                                                                        gACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAAc                                                                         >  1:374294/1‑150 (MQ=255)
                                                                        gACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAAc                                                                         <  1:861347/150‑1 (MQ=255)
                                                                           tGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAg                                                                      >  2:308459/1‑150 (MQ=255)
                                                                           tGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAg                                                                      <  1:1378323/150‑1 (MQ=255)
                                                                             cccTACCCCTTGCGACGTGACGAGGTTCAGCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCTCCAGAATAGCCATACTAAAACGTTTCATAGGACCTCCATTGATTGTGCATTCCTGAATAAGTGTAAACCAGTg                                                                    <  1:960426/150‑1 (MQ=255)
                                                                                tACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGaca                                                                 <  1:312244/150‑1 (MQ=255)
                                                                                    ccTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACAtt                                                             >  2:1344996/1‑150 (MQ=255)
                                                                                      ttGCGATGTGTCGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACAtttt                                                           >  1:237748/1‑150 (MQ=255)
                                                                                      ttGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACAtttt                                                           <  2:249210/150‑1 (MQ=255)
                                                                                       tGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACAttttt                                                          >  2:1551000/1‑150 (MQ=255)
                                                                                        tcGACGTGACGAGGGTCATCTCGACTGTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTg                                                         <  2:72118/149‑1 (MQ=255)
                                                                                          gACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGaa                                                       >  1:194204/1‑150 (MQ=255)
                                                                                          gACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGaa                                                       >  2:414910/1‑150 (MQ=255)
                                                                                             gTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAAcgg                                                    >  1:1488852/1‑150 (MQ=255)
                                                                                               gACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAAcggcg                                                  >  1:29251/1‑150 (MQ=255)
                                                                                                 cGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGGAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTACGTTCATAGTGCATACCTGAATAAGAGTAAACCAGTGACAAATTTTTGAAAggctgc                                                >  1:1113552/1‑150 (MQ=255)
                                                                                                    ggTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTAt                                             >  1:1478155/1‑150 (MQ=255)
                                                                                                      ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTc                                           >  1:1223548/1‑150 (MQ=255)
                                                                                                         aTCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAATGGCGGCTATTCCTa                                        <  1:969365/150‑1 (MQ=255)
                                                                                                          tctcGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAACCGTTTCATAGGACCTCCGTACATTGTGCATTCCTGAATAAGTGTAACCCAGTGACACATTTTTGAACGGCGGCTATTCCTaa                                       >  1:1027006/1‑150 (MQ=255)
                                                                                                           ctcGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTaaa                                      >  1:299322/1‑150 (MQ=255)
                                                                                                               aCTTTTTCTCTGGCAGCTTGATCGCAGGTTGCAACAACCAGCGACAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTTACACATTTTTGAACGGCGGCTATTCCTAAAAGTg                                  <  2:88658/150‑1 (MQ=255)
                                                                                                               aCTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTg                                  <  1:166666/150‑1 (MQ=255)
                                                                                                               aCTTTTTCACTGGCAGCTCGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTg                                  <  1:534715/150‑1 (MQ=255)
                                                                                                                cTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGc                                 >  1:1346001/1‑150 (MQ=255)
                                                                                                                cTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGc                                 >  1:1190129/1‑150 (MQ=255)
                                                                                                                  ttttCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCtt                               <  2:368820/150‑1 (MQ=255)
                                                                                                                    ttCACTGGCAGCTTGTGCGCCGGTTGCAACCACCAGCGCCAGAATAGCCAAACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTAc                             >  2:816489/1‑150 (MQ=255)
                                                                                                                    ttCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTAc                             <  1:1609766/150‑1 (MQ=255)
                                                                                                                      cACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTTAACGGCGGCTATTCCTAAAAGTGCTTACgg                           <  2:1399480/150‑1 (MQ=255)
                                                                                                                      cACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTTAAAGTGCTTACgg                           >  2:62783/1‑150 (MQ=255)
                                                                                                                        cTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTa                         >  1:1198463/1‑150 (MQ=255)
                                                                                                                        cTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTa                         >  2:670185/1‑150 (MQ=255)
                                                                                                                          ggCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTAcg                       <  2:1618762/150‑1 (MQ=255)
                                                                                                                          ggCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTAcg                       >  1:1588979/1‑150 (MQ=255)
                                                                                                                              gCTTGTGCGCCGGTTGCCACAACCAGCGCCAGAATAGCCAGACTAAACCGTTTCATAGGACCTGCGTTCATTGTGCTTTCCTGCATAAGTGTAAACCAGTGCCCCCGTTTTGAACGGCGGCTATTCCTAAAAGTTCTTACGGTaagaagt                   >  2:814179/1‑144 (MQ=255)
                                                                                                                                 tgtgCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAct                <  1:308459/150‑2 (MQ=255)
                                                                                                                                  tggcgcTGGTTGTTGCAACCGGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTTCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAccc               >  1:980834/3‑150 (MQ=255)
                                                                                                                                  gtgCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAccc               <  2:932714/150‑1 (MQ=255)
                                                                                                                                  gtgCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAccc               >  1:1361822/1‑150 (MQ=255)
                                                                                                                                   tgCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAcccc              >  1:46679/1‑150 (MQ=255)
                                                                                                                                   ggcgcTGGTTGTTGCAACCGGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAcccc              >  1:752621/2‑150 (MQ=255)
                                                                                                                                   ggcgcTGGTTGTTGCAACCGGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTAcccc              >  2:1016319/2‑150 (MQ=255)
                                                                                                                                    gcgcTGGTTGTTGCAACCGGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCa             >  2:498795/1‑150 (MQ=255)
                                                                                                                                    gcgaCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTACGTTCATTGTGCATTCCTGAATAAATGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACACCa             >  1:178648/1‑150 (MQ=255)
                                                                                                                                     cgcCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAg            >  2:49944/1‑150 (MQ=255)
                                                                                                                                       ccGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTg          <  1:1609850/150‑1 (MQ=255)
                                                                                                                                        cGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTGc         <  1:473202/150‑1 (MQ=255)
                                                                                                                                          gTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTGCCg       <  2:1259455/150‑1 (MQ=255)
                                                                                                                                               aacaacCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTGCCGCTTTa  >  2:360677/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGGGGTAATGCTTTCAGATCGGGCGAAAACTCCAGACCTTTATCGGTTTCAGTAATGGTGACGCTACCAATTGACTGCCCTACCCCTTGCGACGTGACGAGGTTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATAGCCAGACTAAAACGTTTCATAGGACCTCCGTTCATTGTGCATTCCTGAATAAGTGTAAACCAGTGACACATTTTTGAACGGCGGCTATTCCTAAAAGTGCTTACGGTACGTCGTACCCCAGTGCCGCTTTA  >  NC_000913/1724474‑1724766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: