Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,726,510 | T→G | 6.3% | L163R (CTT→CGT) | nemR → | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,726,510 | 0 | T | G | 6.3% | 265.2 / 6.8 | 91 | L163R (CTT→CGT) | nemR | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
Reads supporting (aligned to +/- strand): ref base T (34/51); new base G (3/3); total (37/54) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.84e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 |
AAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTA > NC_000913/1726363‑1726659 | aaGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCttt < 1:1702028/150‑1 (MQ=255) gtgtGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTAc < 1:179342/150‑1 (MQ=255) gtgtGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTAc < 1:1284104/150‑1 (MQ=255) gtgCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGc > 1:506556/1‑150 (MQ=255) cGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑t > 2:283202/1‑150 (MQ=255) tCTGCCTGAACAAATCCGCCGCGCGTGGCATACAGGTGCTCGCGGGGTGCCCGCCCTGCTCGCGCCGGCGCGGTAACATGGCCGGCACAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGTCACTAGTGCTTTACGCAC‑tgtg < 2:1596775/150‑1 (MQ=255) tGTCTGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGc < 1:90309/150‑1 (MQ=255) tGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGc < 1:666093/150‑1 (MQ=255) tGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCAC‑AGTGCGTAAAGCAC‑ttgtgcc < 1:117384/150‑7 (MQ=255) tGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCAC‑AGTGCGTAAAGCAC‑ttgtgcc < 1:1527603/150‑7 (MQ=255) gTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGATCCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCt > 1:864635/1‑150 (MQ=255) gTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCGTAAAGCAC‑ttgtgcc < 2:465645/150‑7 (MQ=255) tCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCtt < 2:407331/150‑1 (MQ=255) agaagaTATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTgg < 2:506556/150‑1 (MQ=255) gaagaTATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGgc > 1:987426/1‑150 (MQ=255) gaagaTATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGgc < 2:1710363/150‑1 (MQ=255) agaTATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGgcgc > 2:668234/1‑150 (MQ=255) atGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAAt > 2:962588/1‑150 (MQ=255) tGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGGGATCGCCCTGCTCTCGCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATc < 1:291635/150‑1 (MQ=255) cgcAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTg < 2:279174/150‑1 (MQ=255) gcAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGc < 1:1018331/150‑1 (MQ=255) cAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTTTTTAACCTTTTGTGGCGAACCTCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTCGCTTGGCGCGAATCTGCa > 2:1073495/1‑150 (MQ=255) cAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCa > 1:667874/1‑150 (MQ=255) gcgcGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAgg > 2:45456/1‑150 (MQ=255) gcgcGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAgg < 2:791756/150‑1 (MQ=255) aTGGATAAAGGTGCTTGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGc > 2:1242579/1‑142 (MQ=255) aTGGATAAAGGTGCTTGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGc < 1:1242579/142‑1 (MQ=255) ggATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAAt > 1:400317/1‑150 (MQ=255) aTAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAAttt < 1:1255622/150‑1 (MQ=255) ggTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCgcgc > 1:542606/1‑150 (MQ=255) cTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCGTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGtt > 1:1688455/1‑150 (MQ=255) cgcgGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTc > 1:399588/1‑150 (MQ=255) gcggcgTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCg < 1:144171/150‑1 (MQ=255) ggcgTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAg < 2:1566242/150‑1 (MQ=255) ggcgTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAg > 2:995271/1‑150 (MQ=255) gcgTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGc > 1:1583405/1‑150 (MQ=255) tGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACATTTTGTGTCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGTCGCGAATCTGCAGGCCAAAATTTCGAGCAGTTTCGAGCCAc > 2:628000/1‑150 (MQ=255) ccTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGAAGTTTCGAGCCACTGGaaaa < 2:400317/150‑1 (MQ=255) tGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAAcg < 1:1143479/150‑1 (MQ=255) tGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAAcg < 2:667874/150‑1 (MQ=255) gCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAAcgc > 1:1393879/1‑150 (MQ=255) ctctctCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAAcgcg < 1:529274/150‑1 (MQ=255) tctctcAGGCGCTGGAAACTGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGACTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACgcgc < 1:1060576/150‑1 (MQ=255) ctctcAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCAGCAGGCCAAAATTTCGCGAAGTTTCTAGCCACTGGAAAAAGCGCt > 2:767437/1‑150 (MQ=255) ctctcAGGCGCTGGAAAATGGCCGTGAGAAACATTGTTTAACCTGTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCt > 1:544363/1‑150 (MQ=255) cAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGcc > 1:652780/1‑150 (MQ=255) cAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGcc < 2:1390419/150‑1 (MQ=255) ggCGCTGGAAAATGGCCGTGAGAACCATTGTTTTACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCa < 1:403320/150‑1 (MQ=255) cTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTa < 1:1157747/150‑1 (MQ=255) aaaaTGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTaaaaa < 1:1397113/150‑1 (MQ=255) aaTGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAAc < 2:1074273/150‑1 (MQ=255) aaTGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAAc < 2:415204/150‑1 (MQ=255) aaTGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAAc < 1:624802/150‑1 (MQ=255) ccGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTATAAAACattat > 2:647939/1‑150 (MQ=255) cGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACattatt < 2:652780/150‑1 (MQ=255) tGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGc < 2:399588/150‑1 (MQ=255) gagaACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCg < 2:577454/150‑1 (MQ=255) taaCCATTGTTTAAACTTCTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGAc < 2:853156/149‑1 (MQ=255) gaACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGAc > 2:1019321/1‑150 (MQ=255) gaACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGAc > 2:1231301/1‑150 (MQ=255) aaCCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTgg > 1:1104673/1‑112 (MQ=255) aaCCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTgg < 2:1104673/112‑1 (MQ=255) ccATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCg < 2:730620/145‑1 (MQ=255) ccATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCg > 1:730620/1‑145 (MQ=255) ccATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGcc < 1:1019321/150‑1 (MQ=255) ccATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGcc < 1:1231301/150‑1 (MQ=255) ttGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTGCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGc < 1:902513/150‑1 (MQ=255) aaCCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTa < 1:995271/150‑1 (MQ=255) cTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCa < 1:569973/150‑1 (MQ=255) cTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCa > 2:589388/1‑150 (MQ=255) ttttGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATATCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAg < 1:104633/150‑1 (MQ=255) tttGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAgg < 2:848466/150‑1 (MQ=255) tgtgGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCa > 2:327896/1‑150 (MQ=255) tgtgGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCa < 2:227009/150‑1 (MQ=255) tgtgGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCa > 2:614719/1‑150 (MQ=255) gtgGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCAt < 1:1255317/150‑1 (MQ=255) gtgGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCAt > 1:1418805/1‑150 (MQ=255) tgGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCATGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCAtt > 1:1091404/1‑150 (MQ=255) gCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCAtttt > 2:928960/1‑150 (MQ=255) cGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCAttttt < 2:987426/150‑1 (MQ=255) aaCCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTa < 2:1455308/150‑1 (MQ=255) aCCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTAt > 2:1387626/1‑150 (MQ=255) gCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAg < 2:1393879/150‑1 (MQ=255) aCAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCACATGTAAAAAACAATATTTCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAgacg > 2:272556/1‑150 (MQ=255) ggCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAACCGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGAcc > 2:1178884/1‑150 (MQ=255) gccacaGTGCGTAAAGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCg > 1:1111688/6‑150 (MQ=255) ccacaGTGCGTAAAGCACTTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCg > 2:383240/5‑150 (MQ=255) acaAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTCCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCggg < 2:573590/150‑1 (MQ=255) caAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGa < 1:45456/150‑1 (MQ=255) gTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGcc < 1:1599964/150‑1 (MQ=255) ggCTTTACGCAC‑TGTGGGTTGGCTCTAATCTGCAGGCCAAAATTTCTCGCAGTTTCGAGCCACTGTAAAACGCGCTGGCCCATGTAAAAACCATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCATACGACCGGGAGCCt < 2:544363/149‑1 (MQ=255) cTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGTCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAAGATTTTTGCGACGCCTGCCGTTTAGCTGGCATTTTTTATCACCAGACGACCGGGAGCCttt < 1:628000/150‑1 (MQ=255) tttACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTa > 2:1695121/1‑150 (MQ=255) | AAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCAC‑TGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTA > NC_000913/1726363‑1726659 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |