Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,765,162 C→T 7.9% intergenic (‑40/+60) ydiH ← / ← menI uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,765,1620CT 7.9% 269.3 / 4.8 88intergenic (‑40/+60)ydiH/menIuncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
Reads supporting (aligned to +/- strand):  ref base C (38/42);  new base T (2/5);  total (41/47)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAAGTTCTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACC  >  NC_000913/1765013‑1765311
                                                                                                                                                     |                                                                                                                                                     
tCAAGTTCTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATaccac                                                                                                                                                       >  1:409845/1‑150 (MQ=255)
acAATTTATAGTTGTTTCAGACGTATGAGATATTGGGCAGGTTAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTTGGATCAAGTTTAGTAGACATAGCATCCTCGCTATTAGACAAGACCTGCACAGTATaccac                                                                                                                                                       <  1:320504/149‑1 (MQ=255)
   aGTTCTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGt                                                                                                                                                    >  1:685604/1‑150 (MQ=255)
   aGTTCTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCATCGCTTTTAGACAAGACCTGCACAGTATACCACCGt                                                                                                                                                    >  1:696638/1‑150 (MQ=255)
         tgttgttTCAGACGTTTGATATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAATTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACtg                                                                                                                                              <  1:1464973/149‑1 (MQ=255)
          gttgttTCAGATTATTTAGATAATGTGCTGTGGAAATATTGTTCTTGTAACGGAGTAAAAATTCAATGGCAAGCTGGGTGTTATCAATTTGAGTATACATAGCATCCTCGATTTTAGACAAGACCTGCACAGTATACCACCGTTTACtgt                                                                                                                                             <  1:115272/150‑1 (MQ=255)
            tgttTCAGACGTTTGAGATTTTGGGCAGGTGATAGATTGCTCTGGTCACTGGGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATCCCACCGTTTACTGTGc                                                                                                                                           <  1:79002/150‑1 (MQ=255)
            tgttTCAGACGTTTGAGATTTTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGc                                                                                                                                           <  2:1424706/150‑1 (MQ=255)
            tggttCCGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGc                                                                                                                                           <  1:1545513/147‑1 (MQ=255)
             gttTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCa                                                                                                                                          >  2:1662355/1‑150 (MQ=255)
              tttCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAg                                                                                                                                         >  2:1710364/1‑150 (MQ=255)
              tttCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTAAACGGTGGTATACTGTGCAg                                                                                                                                         <  2:1562983/150‑1 (MQ=255)
              ttgcAGACGTTTGAGATATTGGTCAGGTGAAAGATTGCTCTGGTCACGGAGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTAAACGGTGGTATACTGTGCAg                                                                                                                                         <  2:75565/147‑1 (MQ=255)
               ttCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTAAACGGTGGTATACTGTGCAgg                                                                                                                                        <  1:537476/150‑2 (MQ=255)
               ttCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCGGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTAAACGGTGGTATACTGTGCAgg                                                                                                                                        <  2:1044626/150‑2 (MQ=255)
                tCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTAAACGGTGGTATACTGTGCAggt                                                                                                                                       <  2:751969/150‑3 (MQ=255)
                  aGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGaaaa                                                                                                                                     >  2:346854/1‑150 (MQ=255)
                   gACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAAt                                                                                                                                    <  2:1669606/150‑1 (MQ=255)
                    aCGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTCGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATg                                                                                                                                   <  1:1472372/150‑1 (MQ=255)
                      gTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACAACCGTTTACTGTGCAGATAATGAc                                                                                                                                 >  2:259886/1‑150 (MQ=255)
                       ttttAGATAATCGTCAGGTGAAAGATTGCTATGGTCACGGCCTAAAAATTCAATGGACAGCTTGGTGGGATCAAGTTGAGTAGTCATAGCATCCTCGCTTTTAGACAAGACCTGAACAGTATAGCACCGTATACTGTGCAGATAATGAcc                                                                                                                                <  1:98101/150‑1 (MQ=255)
                       tttGAGATATTGGGCAGGTGAAGGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGAcc                                                                                                                                <  1:498740/150‑1 (MQ=255)
                         tGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCaa                                                                                                                              <  2:1567780/150‑1 (MQ=255)
                             atatTGCGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAAATCCATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACCTAGCATCCTCGCTTTTAGACAAGACCAGCACAGTATACCACCGTGTACTGTGCAGATAAAGACCaaaacc                                                                                                                          <  2:78325/150‑3 (MQ=255)
                                 tGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAata                                                                                                                      <  2:641083/150‑1 (MQ=255)
                                   ggCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTTCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATg                                                                                                                    <  1:1506648/150‑1 (MQ=255)
                                   ggCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATg                                                                                                                    >  1:536661/1‑150 (MQ=255)
                                    gCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATAAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGc                                                                                                                   <  1:1520523/150‑1 (MQ=255)
                                       ggTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTc                                                                                                                >  2:680245/1‑150 (MQ=255)
                                       ggTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTc                                                                                                                <  1:471028/150‑1 (MQ=255)
                                        gTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTca                                                                                                               >  2:277441/1‑150 (MQ=255)
                                         tGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTcac                                                                                                              >  1:1005361/1‑150 (MQ=255)
                                          gAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTcaca                                                                                                             >  2:1381791/1‑150 (MQ=255)
                                            aaGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACt                                                                                                           >  1:993127/1‑150 (MQ=255)
                                              gATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACttt                                                                                                         >  2:1002368/1‑150 (MQ=255)
                                                   ctctGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTgg                                                                                                    <  1:1648966/150‑1 (MQ=255)
                                                        gTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACAACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACa                                                                                               >  1:1513624/1‑150 (MQ=255)
                                                         taaCGGAGTAAAAATAAAATCGCCAGCAGGGTCGGATCAAGTTCAGTATACATACCATCCTCCCTTTTAGACAAGACCTCCACATTATAACAACGATAACTCTGCAGATAATGACCAAAAGCAAAAACCTTCACACTATTCTGGtcaaaa                                                                                              >  2:726067/3‑145 (MQ=255)
                                                         tCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACaa                                                                                              >  1:1420060/1‑150 (MQ=255)
                                                          cACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAAc                                                                                             <  1:125748/150‑1 (MQ=255)
                                                           aCGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACg                                                                                            <  2:176058/150‑1 (MQ=255)
                                                              gCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTca                                                                                         >  1:1033173/1‑150 (MQ=255)
                                                              gCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTca                                                                                         >  1:1447719/1‑150 (MQ=255)
                                                               cGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACACCGTcac                                                                                        >  2:985011/1‑150 (MQ=255)
                                                                  aaaaaTTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACaaa                                                                                     >  1:1232320/1‑150 (MQ=255)
                                                                   aaaaTTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACaaaa                                                                                    <  2:1703421/150‑1 (MQ=255)
                                                                    aaaTTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAAt                                                                                   <  1:346854/150‑1 (MQ=255)
                                                                    aaaTTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAAt                                                                                   >  2:38137/1‑150 (MQ=255)
                                                                       ttCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGc                                                                                <  1:1381791/150‑1 (MQ=255)
                                                                                   cTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCg                                                                    <  2:1341163/150‑1 (MQ=255)
                                                                                   cTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCg                                                                    <  2:554040/150‑1 (MQ=255)
                                                                                     gggtgggATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTg                                                                  <  1:1345378/150‑1 (MQ=255)
                                                                                     gggtgggATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTg                                                                  >  2:420587/1‑150 (MQ=255)
                                                                                      ggtgggATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGa                                                                 <  2:385318/150‑1 (MQ=255)
                                                                                       gtgggATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGAc                                                                <  1:1662355/150‑1 (MQ=255)
                                                                                        tgggATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACg                                                               >  2:1474296/1‑150 (MQ=255)
                                                                                        tgggATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACg                                                               <  2:1270091/150‑1 (MQ=255)
                                                                                           gATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAAc                                                            >  2:678126/1‑150 (MQ=255)
                                                                                            aTCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACa                                                           <  1:277441/150‑1 (MQ=255)
                                                                                                aGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTTACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGcac                                                       >  2:388355/1‑150 (MQ=255)
                                                                                                aGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTATACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGcac                                                       <  2:1518596/150‑1 (MQ=255)
                                                                                                     aGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACg                                                  <  1:680245/150‑1 (MQ=255)
                                                                                                       tAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGcc                                                >  1:1165325/1‑150 (MQ=255)
                                                                                                         gACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCt                                              <  2:1033173/150‑1 (MQ=255)
                                                                                                           cATAGCATCCTCGCTTTTAGCCAAGACCTGCACAGTATACCACCGTTAACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCttt                                            >  2:82181/1‑150 (MQ=255)
                                                                                                             tAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTtct                                          <  2:1447719/150‑1 (MQ=255)
                                                                                                             tAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTtct                                          >  2:440839/1‑150 (MQ=255)
                                                                                                              aGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTtctc                                         <  1:420587/150‑1 (MQ=255)
                                                                                                                 aTCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATc                                      >  2:622793/1‑150 (MQ=255)
                                                                                                                   ccTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGa                                    >  2:315964/1‑150 (MQ=255)
                                                                                                                   ccTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGa                                    >  1:337620/1‑150 (MQ=255)
                                                                                                                      cGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGa                                 <  1:1590654/150‑1 (MQ=255)
                                                                                                                        cTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAtt                               >  1:288041/1‑150 (MQ=255)
                                                                                                                          tttAGACAAGACCTTCACAGTATACCACCGTTTACTCTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGAttta                             <  1:259886/150‑2 (MQ=255)
                                                                                                                            tAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCaa                           <  2:729268/150‑1 (MQ=255)
                                                                                                                            tAGACAAGACCTGCACAGGTTTCCACCGTTTACTGTGGAGATAATGACCTAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCACTCGTGGCGAGCAGGACAAGGGCGCTTTCTCAGCGGAGAtgtgat                           >  2:80111/1‑145 (MQ=255)
                                                                                                                              gACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTTACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATc                         >  2:320535/1‑150 (MQ=255)
                                                                                                                                 aaGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGc                      <  2:1598189/150‑1 (MQ=255)
                                                                                                                                    atcTGCACAGTAAACGGTGGTATACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAg                   >  2:1561693/3‑150 (MQ=255)
                                                                                                                                    aCCTGCACAGTATACCACCTTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAg                   <  2:1263762/150‑1 (MQ=255)
                                                                                                                                      cTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGCTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAgcc                 >  2:42415/1‑148 (MQ=255)
                                                                                                                                      cTGCACAGTAAACGGTGGTATACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGAc                 >  2:370940/1‑150 (MQ=255)
                                                                                                                                       tGCCCAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACCGCACCCACGCCCTTTCTCATCGAAGATTTCAAGCTGCCAGAcc                >  2:1491168/1‑150 (MQ=255)
                                                                                                                                       tGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGAcc                >  1:26655/1‑150 (MQ=255)
                                                                                                                                       tGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGAcc                <  1:320535/150‑1 (MQ=255)
                                                                                                                                             gTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGa          <  2:406040/150‑1 (MQ=255)
                                                                                                                                              tataCCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAc         <  1:315964/150‑1 (MQ=255)
                                                                                                                                               ataCCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAcg        >  2:903955/1‑150 (MQ=255)
                                                                                                                                               ataCCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAcg        <  1:1085155/150‑1 (MQ=255)
                                                                                                                                                taCCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAcgc       >  1:1328666/1‑150 (MQ=255)
                                                                                                                                                 accaccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTTACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGAcgcg      <  1:388355/150‑1 (MQ=255)
                                                                                                                                                  ccaccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGa     >  1:1551541/1‑150 (MQ=255)
                                                                                                                                                   caccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGaa    >  2:1088191/1‑150 (MQ=255)
                                                                                                                                                     ccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGGCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAAcc  <  1:618686/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAAGTTCTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACC  >  NC_000913/1765013‑1765311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: