Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,770,204 C→G 5.5% intergenic (+18/‑168) ydiK → / → rprA UPF0118 family inner membrane protein/sRNA antisense activator for RpoS translation, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,770,2040CG 5.5% 362.4 / 9.3 109intergenic (+18/‑168)ydiK/rprAUPF0118 family inner membrane protein/sRNA antisense activator for RpoS translation, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base C (62/41);  new base G (3/3);  total (65/44)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 113 total aligned reads displayed.

TATTGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCC  >  NC_000913/1770057‑1770352
                                                                                                                                                   |                                                                                                                                                    
tATTGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTg                                                                                                                                                    >  1:556908/1‑150 (MQ=255)
 aTTGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgc                                                                                                                                                   >  2:1267183/1‑150 (MQ=255)
   tGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgccg                                                                                                                                                 >  2:624094/1‑150 (MQ=255)
      tCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCt                                                                                                                                              >  2:1000520/1‑150 (MQ=255)
       ccGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTg                                                                                                                                             <  1:1558189/150‑1 (MQ=255)
       ccGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTg                                                                                                                                             >  1:623288/1‑150 (MQ=255)
         ggTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGAt                                                                                                                                           >  2:921739/1‑150 (MQ=255)
          gTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCAGAGCCGCCTGAtg                                                                                                                                          <  2:1247847/150‑2 (MQ=255)
          gTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCAGAGCCGCCTGAtg                                                                                                                                          <  2:272199/150‑2 (MQ=255)
            gcTGGTAGCCGGTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGt                                                                                                                                        <  1:1569346/149‑1 (MQ=255)
              tGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTa                                                                                                                                      <  2:1081215/150‑1 (MQ=255)
               gTTAGCCGTTTCCTGGCGTCCGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGATATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTaa                                                                                                                                     >  2:842996/1‑150 (MQ=255)
                ttAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAac                                                                                                                                    <  1:624094/150‑1 (MQ=255)
                           cTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCTCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGTCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAAtt                                                                                                                         <  2:212458/150‑1 (MQ=255)
                           cTGGAGGCTGTTTGACGCGTGGGTGGAAGAAGACCCGCCGCCGAAGGCCCAACCGGATGCAATTCTCGAAGCACTGGGCGAAATCGAGAAACCGATTAAGTAATTACTCATCAGTATACTCTGCCGCCTGATTTTTAACCACAGCAAAta                                                                                                                         >  1:1062613/1‑149 (MQ=255)
                               cGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGata                                                                                                                     <  1:1190013/150‑1 (MQ=255)
                               cGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGata                                                                                                                     >  1:398664/1‑150 (MQ=255)
                                gTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGatat                                                                                                                    >  1:659233/1‑150 (MQ=255)
                                gTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGatat                                                                                                                    >  2:216388/1‑150 (MQ=255)
                                gTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGatat                                                                                                                    <  2:902405/150‑1 (MQ=255)
                                    gTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgc                                                                                                                >  2:1394620/1‑150 (MQ=255)
                                     tgtGCCGCGTGGGTGGATGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAAGTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgct                                                                                                               <  1:1156111/148‑1 (MQ=255)
                                        gCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGCATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgcttgc                                                                                                            >  1:1637192/1‑150 (MQ=255)
                                        gCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgcttgc                                                                                                            <  2:623288/150‑1 (MQ=255)
                                        gCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATttgcttgc                                                                                                            >  2:263597/1‑150 (MQ=255)
                                        gCCGCGAGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGATGAAATTCTCGAAGAACTTGGCGAAGTCGAGATCCCGAGTAGGTATTTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTGACCACCGCTATGTGATAGttgcttgc                                                                                                            >  1:285083/1‑150 (MQ=255)
                                              tgggtggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTcc                                                                                                      <  1:521448/150‑1 (MQ=255)
                                               gggtggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTccc                                                                                                     >  2:675729/1‑150 (MQ=255)
                                                ggtggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTcccc                                                                                                    <  2:1708588/150‑1 (MQ=255)
                                                   ggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCata                                                                                                 >  2:247382/1‑150 (MQ=255)
                                                   ggAAGAAGTCCCGCCGCCGAAGGACCAACCGGAAGAAATTCTCGAAGAACTTTGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGATCTTCCCCata                                                                                                 <  2:1541203/150‑1 (MQ=255)
                                                    gaagaaGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCatat                                                                                                >  1:1688644/1‑150 (MQ=255)
                                                     aagaagTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAtt                                                                                               >  1:959150/1‑150 (MQ=255)
                                                      agaagTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt                                                                                              >  1:1156316/1‑150 (MQ=255)
                                                      agaagTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt                                                                                              >  1:1081460/1‑150 (MQ=255)
                                                          gTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCt                                                                                          >  1:739201/1‑150 (MQ=255)
                                                            cccGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGc                                                                                        >  1:406974/1‑150 (MQ=255)
                                                              cgccgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCtt                                                                                      <  1:1295058/150‑1 (MQ=255)
                                                              cgccgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCtt                                                                                      >  2:927642/1‑150 (MQ=255)
                                                                  gccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCg                                                                                  >  1:1346797/1‑150 (MQ=255)
                                                                  gccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCg                                                                                  <  2:1310106/150‑1 (MQ=255)
                                                                    cgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGcc                                                                                <  1:1000520/150‑1 (MQ=255)
                                                                     gACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTACCCACCGCGAATTGATATTTGATTGCTCTTCCCCATATTTTCCTGCTTACCGCCa                                                                               >  2:1033942/1‑150 (MQ=255)
                                                                      aCGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCAt                                                                              <  2:959150/150‑1 (MQ=255)
                                                                       cGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCAtt                                                                             <  2:680119/150‑1 (MQ=255)
                                                                           ccAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGc                                                                         >  2:894306/1‑150 (MQ=255)
                                                                            cAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCt                                                                        <  1:1016847/150‑1 (MQ=255)
                                                                            cAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCt                                                                        >  1:355981/1‑150 (MQ=255)
                                                                              aCCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTgg                                                                      >  2:1715000/1‑150 (MQ=255)
                                                                                cGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgta                                                                    <  2:1243407/150‑1 (MQ=255)
                                                                                 ggAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgtag                                                                   >  2:966132/1‑150 (MQ=255)
                                                                                   aagaaATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgtagta                                                                 <  1:1610844/150‑1 (MQ=255)
                                                                                    agaaATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTAc                                                                >  1:51461/1‑150 (MQ=255)
                                                                                     gaaATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTAcc                                                               >  2:573344/1‑150 (MQ=255)
                                                                                         ttCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTc                                                           <  1:1107553/150‑1 (MQ=255)
                                                                                          tctcGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCg                                                          <  2:398664/150‑1 (MQ=255)
                                                                                           ctcGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTTAGCTGGTAGTACCTGTCGc                                                         >  2:306903/1‑150 (MQ=255)
                                                                                            tcGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCa                                                        <  1:1267183/150‑1 (MQ=255)
                                                                                             cGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCaa                                                       >  1:611455/1‑150 (MQ=255)
                                                                                              gaagaaCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCaaa                                                      >  2:768005/1‑150 (MQ=255)
                                                                                               aagaaCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAAt                                                     >  1:1416360/1‑150 (MQ=255)
                                                                                                   aCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCtt                                                 >  1:451341/1‑150 (MQ=255)
                                                                                                        gCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAg                                            >  1:640643/1‑150 (MQ=255)
                                                                                                         cGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGt                                           >  1:716246/1‑150 (MQ=255)
                                                                                                         cGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCACCATATTTTCCTGCTTACAGCAATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGt                                           >  2:280564/1‑150 (MQ=255)
                                                                                                         cGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCACTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGt                                           >  1:1356529/1‑150 (MQ=255)
                                                                                                            aaTCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGtttt                                        >  2:772682/1‑150 (MQ=255)
                                                                                                               cGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaa                                     >  1:912819/1‑150 (MQ=255)
                                                                                                                gagaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaaa                                    <  1:1358424/150‑1 (MQ=255)
                                                                                                                gagaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCACCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaaa                                    >  2:653188/1‑150 (MQ=255)
                                                                                                                 agaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAAc                                   <  2:1482129/150‑1 (MQ=255)
                                                                                                                  gaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACt                                  <  2:1346797/150‑1 (MQ=255)
                                                                                                                   aaaCCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTa                                 >  1:226507/1‑150 (MQ=255)
                                                                                                                      ccGAAAAAGTAATTTCTCATCAGGCGGCTCTGCAGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATg                              <  2:825828/150‑1 (MQ=255)
                                                                                                                       cGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATga                             <  1:966132/150‑1 (MQ=255)
                                                                                                                        gAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACGGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATgag                            >  1:249367/1‑150 (MQ=255)
                                                                                                                        gAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAAc                                   >  2:839445/1‑143 (MQ=255)
                                                                                                                        gAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAAc                                   <  1:839445/143‑1 (MQ=255)
                                                                                                                             aGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGaa                       >  2:792138/1‑150 (MQ=255)
                                                                                                                             aGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACATGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGaa                       >  1:1349497/1‑150 (MQ=255)
                                                                                                                              gTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAAt                      >  2:1529198/1‑150 (MQ=255)
                                                                                                                               tAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATc                     >  1:1063340/1‑150 (MQ=255)
                                                                                                                                aaTTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCttt                                                >  1:421656/1‑122 (MQ=255)
                                                                                                                                    tctcATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATc                <  1:894306/150‑1 (MQ=255)
                                                                                                                                    tctcATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATc                <  2:912819/150‑1 (MQ=255)
                                                                                                                                       aatcaGGCGGCAGCGCCGCCGGATTGTTGACCCCCGCTATTTGATATTTGCTGGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGAAGTACCTGTCGCAAATTCTTTACAGTTTTTACACTAATGAGACGAAACTGATCGAc             >  2:1053587/2‑150 (MQ=255)
                                                                                                                                       aatcaGGCGGCAGAGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACAGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCgcc             >  2:272429/2‑148 (MQ=255)
                                                                                                                                        atcaGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACg            <  2:1081460/150‑1 (MQ=255)
                                                                                                                                        atcaGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACg            <  1:792138/150‑1 (MQ=255)
                                                                                                                                        atcaGGCGGCAGAGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTTCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTATACAGTTTTTAAAGTAATGAGACGAATCTGATCTACg            >  2:1419985/1‑150 (MQ=255)
                                                                                                                                           aGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCaa         <  1:921739/150‑1 (MQ=255)
                                                                                                                                            ggcggcTCTGCCGCCTGATTGTTAACCACCGCTAATTGGTATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCaaa        >  2:35025/1‑150 (MQ=255)
                                                                                                                                            ggcggcTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGCCGCaaa        >  2:474464/1‑150 (MQ=255)
                                                                                                                                            ggcggcTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCaaa        >  1:1598845/1‑150 (MQ=255)
                                                                                                                                            ggcggcTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCaaa        >  1:768888/1‑150 (MQ=255)
                                                                                                                                              cggcTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCaaaaa      <  1:1715000/150‑1 (MQ=255)
                                                                                                                                                 ctctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTc   >  2:1419898/1‑150 (MQ=255)
                                                                                                                                                 ctctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTc   >  2:474196/1‑150 (MQ=255)
                                                                                                                                                  gctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTATTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTcc  <  2:850191/149‑1 (MQ=255)
                                                                                                                                                  gctGCCGCCTGATTGTTAACCACCGCGAATTGATAATTGCTTGCTCTTCCCCTAATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTAAAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTcc  <  2:844498/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TATTGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCC  >  NC_000913/1770057‑1770352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: