Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,789,499 A→C 5.7% intergenic (+18/‑114) aroH → / → ydiE 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/hemin uptake protein HemP homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,789,4990AC 5.7% 234.5 / 6.4 89intergenic (+18/‑114)aroH/ydiE3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/hemin uptake protein HemP homolog
Reads supporting (aligned to +/- strand):  ref base A (53/30);  new base C (3/2);  total (56/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-02

AAGGAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAA  >  NC_000913/1789351‑1789648
                                                                                                                                                    |                                                                                                                                                     
aaGGAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGAc                                                                                                                                                      >  2:1000472/1‑150 (MQ=255)
     aCGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAAt                                                                                                                                                 <  1:834085/150‑1 (MQ=255)
      cGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATg                                                                                                                                                >  2:237817/1‑150 (MQ=255)
         aaaaaaTCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGc                                                                                                                                             <  2:79865/150‑1 (MQ=255)
         aaaaaaTCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCGTCAGGAATGGGc                                                                                                                                             <  1:1253590/150‑1 (MQ=255)
          aaaaaTCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCa                                                                                                                                            <  2:1390843/150‑1 (MQ=255)
            aaaTCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCTAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCGTCAGGAATGGGCacg                                                                                                                                          <  2:457801/150‑3 (MQ=255)
                cgtcgGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGCTACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGTCATTTCTg                                                                                                                                      >  1:1562351/1‑150 (MQ=255)
                 gtcgGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTgc                                                                                                                                     >  1:46466/1‑150 (MQ=255)
                   cgGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTGCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTgcgc                                                                                                                                   <  2:1516137/150‑1 (MQ=255)
                       aGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGTGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACt                                                                                                                               <  1:1061238/150‑1 (MQ=255)
                        gTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACtt                                                                                                                              >  2:104882/1‑150 (MQ=255)
                          cAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACttgt                                                                                                                            >  1:1262954/1‑150 (MQ=255)
                          cAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGCATACCGAACGCCAGGTCGACAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCGTGACGGAAAGGGCATTTCAGCGCAACttgt                                                                                                                            >  1:1534449/1‑150 (MQ=255)
                            gCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACttgttg                                                                                                                          <  2:447094/150‑1 (MQ=255)
                            gCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACttgttg                                                                                                                          <  1:104882/150‑1 (MQ=255)
                              cGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGGAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtc                                                                                                                        >  2:1354180/1‑150 (MQ=255)
                                cTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt                                                                                                                      >  1:914488/1‑150 (MQ=255)
                                         ggTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACaaa                                                                                                             >  1:664291/1‑150 (MQ=255)
                                           tCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAAtt                                                                                                           <  2:1224202/150‑1 (MQ=255)
                                            cAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGATAAAATCGCCTCTGCGGTAGATACCCGCGTCTGAATGCGTGCCCATTGCGGACGGTAGGGGCATTTCTGCGCACCTTGTAGTCTTCGCACCAAATTa                                                                                                          >  2:1271357/1‑150 (MQ=255)
                                             aaTCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTAc                                                                                                         <  1:205450/150‑1 (MQ=255)
                                             aaTCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTAc                                                                                                         <  2:1180691/150‑1 (MQ=255)
                                               tCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACtg                                                                                                       >  2:816576/1‑150 (MQ=255)
                                               tCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACtg                                                                                                       <  1:318511/150‑1 (MQ=255)
                                                ccATTACCGACCCGTGTCTGGGCTCGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACCGAATTGCCATTTCTGCGCAACTTGTTGACTTCTCAACATATTaaatc                                                                                                      >  2:607346/1‑146 (MQ=255)
                                                 cATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACtgct                                                                                                     >  1:429373/1‑150 (MQ=255)
                                                  aTTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACtgctt                                                                                                    >  1:652848/1‑150 (MQ=255)
                                                   ttACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACtgcttg                                                                                                   <  1:889446/150‑1 (MQ=255)
                                                            cGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCa                                                                                          >  2:1253204/1‑150 (MQ=255)
                                                             gtgtCAGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTAATGCTTGCTCTGGTCac                                                                                         >  1:256505/1‑149 (MQ=255)
                                                                  tgggctgggAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCata                                                                                    >  2:1707028/1‑150 (MQ=255)
                                                                    ggctgggAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCataat                                                                                  >  1:1609423/1‑150 (MQ=255)
                                                                    ggctgggAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCataat                                                                                  <  2:1563166/150‑1 (MQ=255)
                                                                        gggAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTg                                                                              <  2:797907/150‑1 (MQ=255)
                                                                           aggaTACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGata                                                                           <  1:1569869/150‑1 (MQ=255)
                                                                               tACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGAtaataa                                                                       <  1:1660960/150‑1 (MQ=255)
                                                                                 ccGAACGCCCGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGa                                                                     >  2:1077394/1‑150 (MQ=255)
                                                                                   gAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAAt                                                                   <  2:1715064/150‑1 (MQ=255)
                                                                                   gAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTACGGACGGAATGGGCATTTCTGCGCAACTTGTTGTCGTCTAAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTTATAATAAGAAt                                                                   >  1:1560746/1‑150 (MQ=255)
                                                                                      cGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCAt                                                                >  1:1687860/1‑150 (MQ=255)
                                                                                               gAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGATTGCGTGCCCCTTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAAGTACTGCTTGCTCTGGGCAGCGATAATTTTGATAATAAGACTCATTGTtatctg                                                       >  1:285631/1‑147 (MQ=255)
                                                                                                aaaaaCTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCa                                                      >  1:680648/1‑150 (MQ=255)
                                                                                                  aaaCTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAat                                                    <  2:914488/150‑1 (MQ=255)
                                                                                                      tCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAattatt                                                >  2:1546627/1‑150 (MQ=255)
                                                                                                       cGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAattatta                                               >  1:586563/1‑150 (MQ=255)
                                                                                                        gCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATTTTGATAATAAGAATCATTGTTATATCAattattat                                              >  1:131987/1‑150 (MQ=255)
                                                                                                        gCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAattattat                                              >  1:37822/1‑150 (MQ=255)
                                                                                                          ctctGCGGTAGATACCAGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATGTCTGCGCAACTTGTTGTCTTCTCAACAAATTTCTGCTTGGTCTGGTCAGCCATAATATTTATAATAAGAATCATTGTTATATAAAttattatta                                            >  1:269149/1‑150 (MQ=255)
                                                                                                            ctGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAAt                                          >  2:1469172/1‑150 (MQ=255)
                                                                                                             tGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAAtt                                         >  1:856017/1‑150 (MQ=255)
                                                                                                              gCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAAttt                                        >  1:1181762/1‑150 (MQ=255)
                                                                                                               gggTAGATTCGCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGGATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCTTTGTTATATCAATTATTATTAAtttt                                       <  2:523658/149‑1 (MQ=255)
                                                                                                               cGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAAtttt                                       >  1:1311873/1‑150 (MQ=255)
                                                                                                                ggTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAAttttt                                      >  1:927510/1‑150 (MQ=255)
                                                                                                                 gTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTTGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTa                                     <  1:894389/150‑1 (MQ=255)
                                                                                                                 gTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTa                                     >  1:1694836/1‑150 (MQ=255)
                                                                                                                   aGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATg                                   >  1:1492407/1‑150 (MQ=255)
                                                                                                                    gATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGc                                  <  1:816576/150‑1 (MQ=255)
                                                                                                                      tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGt                                >  1:621686/1‑150 (MQ=255)
                                                                                                                       aCCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGtt                               <  2:67732/150‑1 (MQ=255)
                                                                                                                       aCCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGtt                               >  1:83752/1‑150 (MQ=255)
                                                                                                                        cccGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTta                              >  2:411088/1‑150 (MQ=255)
                                                                                                                        cccGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTAGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAAAATTGATAATAAGAATCATGGTTATATCAATTATTATTAATTTTTATGCGTta                              >  1:1528981/1‑150 (MQ=255)
                                                                                                                          cGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTtata                            >  2:866512/1‑150 (MQ=255)
                                                                                                                            cTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACg                          <  1:1707028/150‑1 (MQ=255)
                                                                                                                             ttCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACgg                         <  2:652848/150‑1 (MQ=255)
                                                                                                                               cTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGAt                       >  1:1680478/1‑150 (MQ=255)
                                                                                                                                  aaTTCGTGCCCATTCCTGTCGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGGTATACGGATAGc                    <  2:508439/150‑1 (MQ=255)
                                                                                                                                  aaTGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATAATGATAATAAGAATCATTGTTATATAAATTATTATTAATTTTTATGCGTTATAAGGATAGc                    >  2:1483130/1‑150 (MQ=255)
                                                                                                                                    tGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATTCGGATAGCAg                  >  1:387727/1‑150 (MQ=255)
                                                                                                                                    tGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAg                  <  1:1077394/150‑1 (MQ=255)
                                                                                                                                      cGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGaa                >  2:1086494/1‑150 (MQ=255)
                                                                                                                                        tGCCCATTCCGTCAGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGaaaa              >  2:1317607/1‑150 (MQ=255)
                                                                                                                                        tGCCCATTCATGACGTAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACATATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGaaaa              <  1:237817/150‑1 (MQ=255)
                                                                                                                                         gCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAAc             >  2:706722/1‑150 (MQ=255)
                                                                                                                                         gCCCATTCCTGACGGAATGGGCATTTCTGCGCAACATGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTTATTTTTATGCGTTATACGGATAGCAGAAAAc             >  1:1505825/1‑150 (MQ=255)
                                                                                                                                         gCCCATTCCGTCGGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAAc             >  1:815980/1‑150 (MQ=255)
                                                                                                                                         gCCCATTCCGTCAGGAATGGGCATTTCTGCGCAACTTGATGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGaaaaa             >  1:454483/1‑149 (MQ=255)
                                                                                                                                          cccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACt            >  2:1276162/1‑150 (MQ=255)
                                                                                                                                          cccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATAAGGATAGCAGAAAACt            >  2:1356707/1‑150 (MQ=255)
                                                                                                                                          cccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATACTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACt            <  2:46466/150‑1 (MQ=255)
                                                                                                                                            cATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCa          <  1:1469172/150‑1 (MQ=255)
                                                                                                                                                ccTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCCCGcc      >  2:167721/1‑150 (MQ=255)
                                                                                                                                                ccTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGcc      <  1:1253204/150‑1 (MQ=255)
                                                                                                                                                ccTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGcc      >  2:850148/1‑150 (MQ=255)
                                                                                                                                                  tGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTg    >  1:1374361/1‑150 (MQ=255)
                                                                                                                                                   gACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGa   <  1:1354180/150‑1 (MQ=255)
                                                                                                                                                   gACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGa   >  1:1407765/1‑150 (MQ=255)
                                                                                                                                                    aCGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGATTCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGaa  <  2:325172/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAGGAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGTCAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAA  >  NC_000913/1789351‑1789648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: