Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,807,316 G→A 6.5% intergenic (+17/‑84) pfkB → / → ydiZ 6‑phosphofructokinase II/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,807,3160GA 6.5% 384.2 / 8.6 122intergenic (+17/‑84)pfkB/ydiZ6‑phosphofructokinase II/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (62/52);  new base A (4/4);  total (66/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 123 total aligned reads displayed.

AATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAA  >  NC_000913/1807174‑1807465
                                                                                                                                              |                                                                                                                                                     
aaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                                                                                                                <  1:33940/150‑1 (MQ=255)
aaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                                                                                                                >  1:421404/1‑150 (MQ=255)
     ctctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGGTACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAat                                                                                                                                           <  2:149031/150‑1 (MQ=255)
      tctctTGAAGAGATGGTTCGTTTTGGCGTCGCTGCGGGGAGTGCCGCCACACTCAATCAGCGAACACGTCTGGGCTCCCATGACGATACGCAAAAAATTTCCGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatc                                                                                                                                          <  2:870390/150‑1 (MQ=255)
      tctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATATGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatg                                                                                                                                          <  2:483781/150‑2 (MQ=255)
      tctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatg                                                                                                                                          <  1:917009/150‑2 (MQ=255)
       ctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatgt                                                                                                                                         <  1:1196677/150‑3 (MQ=255)
        tctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatcat                                                                                                                                        <  2:661193/150‑1 (MQ=255)
         ctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatcatc                                                                                                                                       <  2:1585827/150‑1 (MQ=255)
         ctTGAAGAGATGGTTCGGTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatcatc                                                                                                                                       <  1:1329230/150‑1 (MQ=255)
          ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAAtcatca                                                                                                                                      <  1:557621/150‑1 (MQ=255)
          ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAAtcatca                                                                                                                                      >  1:544514/1‑150 (MQ=255)
           tGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatc                                                                                                                                          <  1:833971/145‑1 (MQ=255)
            gAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAcc                                                                                                                                    <  2:1138221/150‑1 (MQ=255)
                 gaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCGCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTACCAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCt                                                                                                                               >  2:1694573/1‑150 (MQ=255)
                    ggTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTc                                                                                                                            >  2:1416959/1‑150 (MQ=255)
                    ggTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTc                                                                                                                            >  1:203378/1‑150 (MQ=255)
                       tCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAACACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGc                                                                                                                         <  1:371198/150‑1 (MQ=255)
                       tCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGc                                                                                                                         <  1:1416959/150‑1 (MQ=255)
                        cGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCaa                                                                                                                       >  1:724782/1‑150 (MQ=255)
                             tGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcgcg                                                                                                                   <  1:223422/150‑1 (MQ=255)
                                       gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGAc                                                                                                         <  2:1655367/150‑1 (MQ=255)
                                          gggAGTGCAGCCTCACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTAt                                                                                                      >  2:647577/1‑150 (MQ=255)
                                           ggAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATg                                                                                                     <  2:96807/150‑1 (MQ=255)
                                             aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCt                                                                                                   >  1:670537/1‑150 (MQ=255)
                                               ggCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCa                                                                                                 <  2:825673/149‑1 (MQ=255)
                                                 cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaaa                                                                                               >  2:913786/1‑150 (MQ=255)
                                                   gCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAg                                                                                             >  1:638986/1‑150 (MQ=255)
                                                     cacaCTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTc                                                                                           >  2:448935/1‑150 (MQ=255)
                                                     cacaCTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGATACTCCGACTATGCTCAAAAGta                                                                                           >  2:1165193/1‑149 (MQ=255)
                                                       cacTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAt                                                                                         >  1:1103289/1‑150 (MQ=255)
                                                       cacTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAt                                                                                         >  2:1428562/1‑150 (MQ=255)
                                                        acTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtg                                                                                        >  2:489644/1‑150 (MQ=255)
                                                         cTCAATCAGGGAACACGTCTGTGCTCCCATGCCGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCCGCAGTGGGGGAATCATCCCCCACCGGTCGGCAACGCGTTTCTCCGCCTAGGCTCAAAAGGCAtgt                                                                                       >  2:1209033/1‑150 (MQ=255)
                                                         cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                       <  2:1080931/150‑1 (MQ=255)
                                                         cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                       >  1:148197/1‑150 (MQ=255)
                                                         cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                       <  2:761922/150‑1 (MQ=255)
                                                         cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTAGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgg                                                                                       >  1:1071783/1‑149 (MQ=255)
                                                          tCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgtg                                                                                      <  1:913786/150‑1 (MQ=255)
                                                           cAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGa                                                                                     >  1:509679/1‑150 (MQ=255)
                                                            aaTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTc                                                                                                  <  1:641860/136‑1 (MQ=255)
                                                            aaTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTc                                                                                                  >  2:641860/1‑136 (MQ=255)
                                                             aTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATa                                                                                   <  2:421404/150‑1 (MQ=255)
                                                             aTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATa                                                                                   <  2:802120/150‑1 (MQ=255)
                                                              tCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATaa                                                                                  <  2:39127/150‑1 (MQ=255)
                                                                 gggAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACaa                                                                               >  1:32133/1‑150 (MQ=255)
                                                                   gAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAg                                                                             >  2:1401586/1‑150 (MQ=255)
                                                                      cacGTCTGTGCTCCCATGACGATACGCAAATAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgggg                                                                          <  2:924230/150‑1 (MQ=255)
                                                                      cacGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgggg                                                                          <  1:1642560/150‑1 (MQ=255)
                                                                       acagacgtgtTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGt                                                                         >  1:503021/7‑150 (MQ=255)
                                                                        cGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTg                                                                        >  2:1349915/1‑150 (MQ=255)
                                                                        cGTCTGTGCTCCCATGACGATACGCAAAAAATCTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTg                                                                        <  1:1264208/150‑1 (MQ=255)
                                                                          tCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGaa                                                                      >  2:968971/1‑150 (MQ=255)
                                                                          tCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAACCACCTGTCGGCAAAGCGTTACTACGACTATGCTCAAAAGTCATGTGATTACAATGGGGTGaa                                                                      >  1:95960/1‑150 (MQ=255)
                                                                          tCTGTGATCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGGTAACAAAAACATTCCCCCAGCATTGCGGGATTCATCACCAACCTGTCGGCAACACCTTTCTCCGACTAAGCTCAAAAGTCATGTGATAACAAAGGGGAGaa                                                                      >  1:71642/1‑150 (MQ=255)
                                                                           cTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAAc                                                                     <  1:1400165/150‑1 (MQ=255)
                                                                              tgCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCCCCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTAt                                                                  >  2:1674991/1‑150 (MQ=255)
                                                                                  cccATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGcc                                                              >  2:193488/1‑150 (MQ=255)
                                                                                  cccATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGcc                                                              <  1:1430958/150‑1 (MQ=255)
                                                                                    cATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAg                                                            >  2:417671/1‑150 (MQ=255)
                                                                                    cATGACGATAAGCAAAAAATTTTCGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATAATCACCAACCTGTCGGCAACGCCTTTCTCCGACTATGCTCAAAAGTCATGTGATAAAAAAGGTGTGAACTATGGcaat                                                            >  2:147452/1‑147 (MQ=255)
                                                                                      tGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTg                                                          >  1:902880/1‑150 (MQ=255)
                                                                                           aTACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGAt                                                     >  2:873571/1‑150 (MQ=255)
                                                                                             aCGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATATCAAAGGGGTGAACTATGGCCAGTGGCGATCt                                                   <  1:1105336/150‑1 (MQ=255)
                                                                                              cGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGAGATCtt                                                  >  2:203797/1‑150 (MQ=255)
                                                                                               gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGGCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTg                                                 <  1:1366148/150‑1 (MQ=255)
                                                                                                cAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGt                                                >  2:1202975/1‑150 (MQ=255)
                                                                                                cAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGAATATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGt                                                >  2:1087009/1‑150 (MQ=255)
                                                                                                 aaaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTc                                               >  1:894741/1‑150 (MQ=255)
                                                                                                  aaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGATTCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCGCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTcc                                              <  2:569419/150‑1 (MQ=255)
                                                                                                       tttACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGCTGAACAATGGCCAGTGGCGATCTTGTCCGtttt                                         >  2:394042/1‑148 (MQ=255)
                                                                                                            gCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGTCGATCTTGTCCGTTATGTCAt                                    <  1:91810/150‑1 (MQ=255)
                                                                                                            gCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCAt                                    <  2:794297/150‑1 (MQ=255)
                                                                                                             cTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCTACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATa                                   <  1:300497/150‑1 (MQ=255)
                                                                                                             cTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATa                                   >  1:1652393/1‑150 (MQ=255)
                                                                                                                 ccTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGCCTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGTTCTTGTCCGTTATGTCATAACCg                               >  1:1004239/1‑150 (MQ=255)
                                                                                                                 ccTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAACAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCg                               <  1:1694573/150‑1 (MQ=255)
                                                                                                                  cTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGt                              >  2:482143/1‑150 (MQ=255)
                                                                                                                        cGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGtt                        >  1:397714/1‑150 (MQ=255)
                                                                                                                        cGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGtt                        >  1:14930/1‑150 (MQ=255)
                                                                                                                           tAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCa                     <  1:1202975/150‑1 (MQ=255)
                                                                                                                            aaCAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCCTAACCGTCATGTTGCAt                    >  2:308222/1‑150 (MQ=255)
                                                                                                                            aaCAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCAt                    <  1:873571/150‑1 (MQ=255)
                                                                                                                             aCAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATg                   >  2:1507696/1‑150 (MQ=255)
                                                                                                                               gtgatgaTTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGCTCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAg                 >  1:279642/7‑150 (MQ=255)
                                                                                                                                aaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAgg                >  2:337795/1‑150 (MQ=255)
                                                                                                                                aaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAgg                <  1:1674991/150‑1 (MQ=255)
                                                                                                                                  atgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGAt              >  2:1660771/4‑150 (MQ=255)
                                                                                                                                  atgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGAt              >  1:431040/4‑150 (MQ=255)
                                                                                                                                     aTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGTTACa           >  2:1080619/1‑150 (MQ=255)
                                                                                                                                        cccccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTg        <  2:902880/150‑1 (MQ=255)
                                                                                                                                        cccccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTg        >  1:1121886/1‑150 (MQ=255)
                                                                                                                                        cccccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCCATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTg        <  2:724782/150‑1 (MQ=255)
                                                                                                                                         ccccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGa       >  1:979476/1‑150 (MQ=255)
                                                                                                                                          cccAGCATTGGGGGAATCATCACCACCCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGAc      <  1:1209033/150‑1 (MQ=255)
                                                                                                                                          cccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGAc      <  1:417671/150‑1 (MQ=255)
                                                                                                                                           ccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACt     <  2:148197/150‑1 (MQ=255)
                                                                                                                                             aGCTTTGGGGTATTAATCACCAACCTGTCGTCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCATCATGTTGCATGAGGATACATTGACTGa   <  2:95960/150‑1 (MQ=255)
                                                                                                                                             aGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGa   <  1:968971/150‑1 (MQ=255)
                                                                                                                                              gCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGaa  <  2:279642/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
AATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAA  >  NC_000913/1807174‑1807465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: