Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,871,781 T→G 5.6% L466R (CTT→CGT)  yeaI → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,871,7810TG 5.6% 303.4 / 7.9 107L466R (CTT→CGT) yeaIputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (51/50);  new base G (3/3);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

Only 100 of 109 total aligned reads displayed.

ATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAAT  >  NC_000913/1871634‑1871930
                                                                                                                                                   |                                                                                                                                                     
aTAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTg                                                                                                                                                     >  1:1683595/1‑150 (MQ=255)
 tAGTGCTCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGt                                                                                                                                                    >  2:1531679/1‑150 (MQ=255)
  aGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTc                                                                                                                                                   <  1:1092537/150‑1 (MQ=255)
  aGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACAAGCGAGATATTACGCGTGTc                                                                                                                                                   <  2:461289/150‑1 (MQ=255)
  aGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAAAAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTc                                                                                                                                                   >  1:870524/1‑150 (MQ=255)
   gTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCa                                                                                                                                                  >  2:1067942/1‑150 (MQ=255)
   gTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCa                                                                                                                                                  >  1:474291/1‑150 (MQ=255)
        cAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACAAGCGAGATATTACGCGTGTCacccgt                                                                                                                                             <  2:1140854/150‑6 (MQ=255)
          ggCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGa                                                                                                                                           <  2:638411/150‑1 (MQ=255)
          ggCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACAAGCGAGATATTACGCGTGTCacccgttg                                                                                                                                           <  1:1646913/150‑8 (MQ=255)
          ggCAAAAATCATTGCGGAACGGATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGa                                                                                                                                           <  1:432542/150‑1 (MQ=255)
           gCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGaa                                                                                                                                          >  1:647197/1‑150 (MQ=255)
            cAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAg                                                                                                                                         >  1:822385/1‑150 (MQ=255)
              aaaaTCATTGCGGAACGAATGAGTAAAAATGTCGAACTCCAGACCGGCTTTATTAAAATATAAGATGTTCATGAACAAATGACCATCAGTAATGGCACTGTTTTTTCAACGGGTGACACGCGTAAAAACTCGCTTCTCATGACGGAAGCa                                                                                                                                       >  2:1538161/1‑150 (MQ=255)
                        cGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCt                                                                                                                             <  2:81263/150‑1 (MQ=255)
                               tATGAGTAAAAATGTCGAACTCCTGCCCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACgcga                                                                                                                      <  2:1604703/150‑1 (MQ=255)
                                   cGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACgcgaagcg                                                                                                                  >  1:1704945/1‑150 (MQ=255)
                                   cGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACgcgaagcg                                                                                                                  >  2:436641/1‑150 (MQ=255)
                                    gTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACgcgaagcga                                                                                                                 >  2:1285195/1‑150 (MQ=255)
                                      aaaaaTGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTAcg                                                                                                                         >  1:1292783/1‑140 (MQ=255)
                                       aaaaTGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGaaaa                                                                                                              >  1:1598374/1‑150 (MQ=255)
                                           tGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGa                                                                                                          >  1:1037331/1‑150 (MQ=255)
                                             tCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAgg                                                                                                        <  2:952082/150‑1 (MQ=255)
                                              cGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAggg                                                                                                       >  2:410050/1‑150 (MQ=255)
                                               gAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAgggg                                                                                                      >  1:223748/1‑150 (MQ=255)
                                                aaCTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGCCCATCAGTAATGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAggggg                                                                                                     <  1:338028/150‑1 (MQ=255)
                                                 aCTCCTGACAGGATTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTAATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAgggtgg                                                                                                    <  2:335307/150‑1 (MQ=255)
                                                  cTCCTGACCGGCTTTAGTATCAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGc                                                                                                   >  2:241996/1‑150 (MQ=255)
                                                  cTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGc                                                                                                   <  1:436641/150‑1 (MQ=255)
                                                   tCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGca                                                                                                  <  1:368069/150‑1 (MQ=255)
                                                   tCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGca                                                                                                  <  1:616231/150‑1 (MQ=255)
                                                         ccGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAg                                                                                            <  2:566338/150‑1 (MQ=255)
                                                         ccGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAg                                                                                            >  2:1250277/1‑150 (MQ=255)
                                                          cGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGt                                                                                           <  1:1336632/150‑1 (MQ=255)
                                                            gCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACCCGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGa                                                                                         >  1:833344/1‑150 (MQ=255)
                                                            ccTTTAGAAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGACGGGGGCAACAAAGTGa                                                                                         >  2:242839/2‑150 (MQ=255)
                                                             cTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGat                                                                                        <  1:1193731/150‑1 (MQ=255)
                                                             cTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGat                                                                                        <  1:1391554/150‑1 (MQ=255)
                                                              tttAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGatt                                                                                       >  1:1587686/1‑150 (MQ=255)
                                                                tAGTTACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGattat                                                                                     <  2:1683595/150‑1 (MQ=255)
                                                                 aGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGattatt                                                                                    >  2:455226/1‑150 (MQ=255)
                                                                 aGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTCTTCAACGGGTGACACGCGTAATACCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGattatt                                                                                    <  1:1121142/150‑1 (MQ=255)
                                                                 aGTAACAGATATGATGTTCCTGAAAAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACAAGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGAGAGGGGGGCAACAAAGAGattatt                                                                                    >  1:89308/1‑150 (MQ=255)
                                                                   tAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATtca                                                                                  <  2:1603262/150‑1 (MQ=255)
                                                                      cAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACTCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATtcagca                                                                               >  1:1153231/1‑150 (MQ=255)
                                                                       aGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTcatcat                                                                              <  2:870524/150‑1 (MQ=255)
                                                                        gATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCata                                                                             >  2:1428707/1‑150 (MQ=255)
                                                                            tgatgTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTa                                                                         >  1:975683/1‑150 (MQ=255)
                                                                             gatgTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTaa                                                                        <  2:1037331/150‑1 (MQ=255)
                                                                               tgTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACATGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGt                                                                      >  1:482156/1‑150 (MQ=255)
                                                                               tgTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGt                                                                      >  2:262980/1‑150 (MQ=255)
                                                                                     tGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCaaaa                                                                >  2:1190411/1‑150 (MQ=255)
                                                                                      gAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCaaaaa                                                               >  1:751250/1‑150 (MQ=255)
                                                                                        aCAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAata                                                             <  1:1630664/150‑1 (MQ=255)
                                                                                        aCAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTAAAGTGCAAAAata                                                             >  2:807807/1‑150 (MQ=255)
                                                                                         cAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGCGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAatat                                                            >  2:150597/1‑150 (MQ=255)
                                                                                           aaTGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATAtta                                                          >  2:181528/1‑149 (MQ=255)
                                                                                                ccATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGGGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCTGAGc                                                     <  2:822385/150‑1 (MQ=255)
                                                                                                ccATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGc                                                     >  2:1653385/1‑150 (MQ=255)
                                                                                                 cATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGcc                                                    >  1:761025/1‑150 (MQ=255)
                                                                                                 cATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCACGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGcc                                                    >  2:671719/1‑150 (MQ=255)
                                                                                                    cAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATg                                                 >  1:816428/1‑150 (MQ=255)
                                                                                                     aGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGAAAAAATATTCAGAGCCATGc                                                >  2:869461/1‑150 (MQ=255)
                                                                                                      gTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCt                                               >  2:885055/1‑150 (MQ=255)
                                                                                                          tGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCttttt                                           >  1:976637/1‑150 (MQ=255)
                                                                                                           ggCACTGTTTTTTCAACTGGTTACACGCTTAATATCTCGCTTTTCATGACGGAATCATATAAAGCCATCCGCGAAGCGAAAAGCGAGGGGGGCTACAAAGTGATTATTCATAATATTTAAGTGAAAAAATTTTCAGATCCATGCTTTTTg                                          <  1:1538161/150‑1 (MQ=255)
                                                                                                           ggCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTg                                          >  2:1546880/1‑150 (MQ=255)
                                                                                                            gCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCGTATTTAAGTGCAATAATATTCAGAGCCATGCTTTTTGc                                         >  2:1448473/1‑150 (MQ=255)
                                                                                                             cACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCg                                        <  1:1428707/150‑1 (MQ=255)
                                                                                                               cGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTg                                      <  2:1345043/150‑1 (MQ=255)
                                                                                                               cGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTg                                      <  1:1225294/150‑1 (MQ=255)
                                                                                                                ggTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTg                                          >  2:44092/1‑145 (MQ=255)
                                                                                                                ggTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTg                                          <  1:44092/145‑1 (MQ=255)
                                                                                                                ggTTTTTACAACGGGTGACACGCGTAATATCTCTCTTGTAATGACGGAATCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTgg                                     <  2:89308/150‑1 (MQ=255)
                                                                                                                 gTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGc                                    <  2:474291/150‑1 (MQ=255)
                                                                                                                   tttttCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCtt                                  >  1:140574/1‑150 (MQ=255)
                                                                                                                     tttCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCtttt                                >  1:396589/1‑150 (MQ=255)
                                                                                                                      ttCTACGGGTGTCACGCGTAATATCTCGCTTGTCATGACTGAAGCAGATAAAGGCTTTCGCGAAGCGAAAAGCGATGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGTTTTTTGCGTGTCTTTTg                               <  1:1322047/150‑1 (MQ=255)
                                                                                                                        tccgtcatGACAAGCGAGATATTACGCGTGTCATGACGGAAGCAGATAAAGCATTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCa                             >  1:965414/8‑150 (MQ=255)
                                                                                                                        cAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCa                             <  2:833344/150‑1 (MQ=255)
                                                                                                                        cAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCa                             >  2:843876/1‑150 (MQ=255)
                                                                                                                         aaCGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGACAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCAt                            <  1:1285195/150‑1 (MQ=255)
                                                                                                                            tcatGACAAGCGAGATATTACGCGTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACa                         >  1:855081/4‑150 (MQ=255)
                                                                                                                            gtgtgtCACGCGTAATATCTCGTTTGTCATGACGGAAGCTTATAAAGCCTTACGGGAATCGAAAAGCGTGGGGGGCATCAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGGGTGGCTTTTGCATAct                         <  1:242839/148‑2 (MQ=255)
                                                                                                                            gggTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATAct                         <  1:671719/150‑2 (MQ=255)
                                                                                                                            gggTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACa                         >  2:17054/1‑150 (MQ=255)
                                                                                                                             catGACAAGCGAGATATTACGCGTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACaa                        >  2:212910/3‑150 (MQ=255)
                                                                                                                                gACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAATTGATTATTAATAATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAAttt                     >  2:956462/1‑150 (MQ=255)
                                                                                                                                  cacGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATttat                   <  1:410050/150‑1 (MQ=255)
                                                                                                                                   acGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATTTTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATttatt                  <  1:1190411/150‑1 (MQ=255)
                                                                                                                                   acGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATttatt                  <  2:976637/150‑1 (MQ=255)
                                                                                                                                    cgcgTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATttatta                 <  1:1250277/150‑1 (MQ=255)
                                                                                                                                      cgTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTtattact               <  1:262980/150‑1 (MQ=255)
                                                                                                                                       gTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTtattacta              <  2:140574/150‑1 (MQ=255)
                                                                                                                                       gTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTtattacta              <  2:223748/150‑1 (MQ=255)
                                                                                                                                        tAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTtataactat             <  2:1587686/150‑1 (MQ=255)
                                                                                                                                         aaTATCTTGCTTGTCATTACGGCATCATTTAAAGCCTTACGCGTATCGTAAAGCGTTGGGGGCAACAAAGTGTTTAATCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTtattactatt            <  1:807807/150‑1 (MQ=255)
                                                                                                                                          atatCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTtattactatta           >  2:1128441/1‑150 (MQ=255)
                                                                                                                                                   ttGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCATAGCCATGCTTTTTGAGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAAt  >  2:535206/1‑150 (MQ=255)
                                                                                                                                                   ttGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAAt  <  2:751250/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAAT  >  NC_000913/1871634‑1871930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: