Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,885,810 T→A 5.2% intergenic (+21/‑35) yeaW → / → yeaX putative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster/putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,885,8100TA 5.2% 282.7 / 3.5 95intergenic (+21/‑35)yeaW/yeaXputative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster/putative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand):  ref base T (43/47);  new base A (3/2);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

GAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCG  >  NC_000913/1885663‑1885959
                                                                                                                                                   |                                                                                                                                                     
caaaGGGCTGAAATCGCGTGGCTATCGTGGGCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTCTCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTcc                                                                                                                                                     <  1:834887/149‑1 (MQ=255)
    gggCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTccgccg                                                                                                                                                 >  2:1442190/1‑150 (MQ=255)
      gCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGAAAATACcgccgcc                                                                                                                                               <  2:1152667/150‑2 (MQ=255)
        tGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTgg                                                                                                                                             >  1:283161/1‑150 (MQ=255)
        tGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGAAAATACcgccgccga                                                                                                                                             <  1:1495062/150‑4 (MQ=255)
            aTCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCAACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTg                                                                                                                                         >  1:319339/1‑150 (MQ=255)
              cgcgTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAt                                                                                                                                       <  1:958301/150‑1 (MQ=255)
              cgcgTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAtt                                                                                                                                      >  1:216534/1‑150 (MQ=255)
                cgTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTTATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAttt                                                                                                                                     >  1:808098/1‑150 (MQ=255)
                 gTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAtttt                                                                                                                                    >  2:509455/1‑150 (MQ=255)
                   ggCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTTGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTg                                                                                                                                  <  1:1584660/150‑1 (MQ=255)
                   ggCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTCGTGGCATTTCCGAACATGGTATCGCCCATTTCCATCATCTGCGGGCGCAGGTGGTTATGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTg                                                                                                                                  <  1:193649/150‑1 (MQ=255)
                   ggCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTg                                                                                                                                  >  2:1208665/1‑150 (MQ=255)
                     cTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAt                                                                                                                                <  1:1442190/150‑1 (MQ=255)
                     cTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGGAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAt                                                                                                                                <  1:167024/150‑1 (MQ=255)
                       aTCGTGGTCAGGGGCGCATCATGGCCTACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATgg                                                                                                                              <  2:319339/150‑1 (MQ=255)
                            ggTCAGGGGCGCATCATGGCGGACAGTAGCGGTTTGGGCATTTCCGAACATGGTATCGCCCATTTCCATACTCTGCTGGCGCAGGTGTTTAAGGACTAATGTCATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTAc                                                                                                                         <  2:669267/150‑1 (MQ=255)
                             gTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAGGGAGTACa                                                                                                                        >  2:138434/1‑150 (MQ=255)
                               cAGGGGCGCATCATGGCTGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGc                                                                                                                      >  2:1585177/1‑150 (MQ=255)
                                aGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCa                                                                                                                     >  1:1402610/1‑150 (MQ=255)
                                 ggggCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCaa                                                                                                                    >  1:200542/1‑150 (MQ=255)
                                      gcATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCa                                                                                                               <  1:776834/150‑1 (MQ=255)
                                           atGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTa                                                                                                          >  2:1346/1‑150 (MQ=255)
                                             ggCTGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATc                                                                                                        <  1:1585177/150‑1 (MQ=255)
                                               ccGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCaa                                                                                                      >  2:888478/1‑150 (MQ=255)
                                                 gACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAAt                                                                                                    >  2:212226/1‑150 (MQ=255)
                                                  aCAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATg                                                                                                   >  1:76519/1‑150 (MQ=255)
                                                    aGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGTCTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGtt                                                                                                 <  2:761156/150‑1 (MQ=255)
                                                    aGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGGTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGtt                                                                                                 >  1:1056009/1‑150 (MQ=255)
                                                    aGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGtt                                                                                                 <  1:474907/150‑1 (MQ=255)
                                                       tgCGGTAGTGGCATTTGCGAACATCGTATCGCCCATTTCCATATTCTGCGCGCGCAGGTGTTTTAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAAGGAGTACAGCAATGTCAGACAATCAAATGTTTgt                                                                                              <  2:918510/149‑2 (MQ=255)
                                                       aGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGa                                                                                              <  1:801850/150‑1 (MQ=255)
                                                          ggTAGTGGCATTTCCGAACATGGTATAGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGt                                                                                           >  1:810291/1‑150 (MQ=255)
                                                              gTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACa                                                                                        <  2:1134042/149‑1 (MQ=255)
                                                              gTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACa                                                                                        >  1:1134042/1‑149 (MQ=255)
                                                              gTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAg                                                                                       >  2:741485/1‑150 (MQ=255)
                                                               tGGTATTTCCGAACATGGTATCGCCCATTTCCATAATGTGCTGGGGCAGGTGTTTAAGGACTATTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAgg                                                                                      <  1:1149132/150‑1 (MQ=255)
                                                                ggCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGt                                                                                     <  1:398216/150‑1 (MQ=255)
                                                                 gCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTg                                                                                    >  1:423932/1‑150 (MQ=255)
                                                                   aTTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAg                                                                                  <  1:1208665/150‑1 (MQ=255)
                                                                    tttCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGc                                                                                 >  1:1632567/1‑150 (MQ=255)
                                                                      tCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTTATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCa                                                                               <  2:808098/150‑1 (MQ=255)
                                                                        cGAACATGGGATCGCCAATTTCCATAATCTGCTTGCGCAGGTGTTAAAGGACTAATGAAAGCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGACTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAgg                                                                             <  2:869574/150‑1 (MQ=255)
                                                                           aCATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTg                                                                          <  1:509455/150‑1 (MQ=255)
                                                                               ggTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAAcc                                                                      <  2:567848/150‑1 (MQ=255)
                                                                               ggTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAAcc                                                                      <  2:1698697/150‑1 (MQ=255)
                                                                                 tATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAAcccc                                                                    >  1:577554/1‑150 (MQ=255)
                                                                                  aTCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCt                                                                   <  1:481468/150‑1 (MQ=255)
                                                                                  aTCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCt                                                                   >  1:1667850/1‑150 (MQ=255)
                                                                                  aTCGCCCAATTCCATTATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCt                                                                   <  2:447606/150‑1 (MQ=255)
                                                                                     gCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTAc                                                                <  2:1402610/150‑1 (MQ=255)
                                                                                       ccATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCg                                                              <  2:283161/150‑1 (MQ=255)
                                                                                       ccATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCg                                                              <  2:76519/150‑1 (MQ=255)
                                                                                           ttCCATAATCTGCTGGCGCATGTGATTAAGGACTAATGACATCGGCGGCGGTATTTTCGGCCGCGGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAATTACATGTGAGCCAGGTTGTACCCCTTACCGAACa                                                          <  2:1187433/150‑1 (MQ=255)
                                                                                           ttCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACa                                                          >  2:1247832/1‑150 (MQ=255)
                                                                                           ttCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACa                                                          >  1:1396640/1‑150 (MQ=255)
                                                                                             ccATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTAGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAgg                                                        <  1:981859/150‑1 (MQ=255)
                                                                                              cATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGt                                                       >  2:1439220/1‑150 (MQ=255)
                                                                                              cATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGt                                                       >  1:224910/1‑150 (MQ=255)
                                                                                               aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTg                                                      <  2:238045/150‑1 (MQ=255)
                                                                                               aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTg                                                      <  1:795424/150‑1 (MQ=255)
                                                                                                 aaTCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGaa                                                    <  1:277335/150‑1 (MQ=255)
                                                                                                  aTCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAAc                                                  >  2:1343240/1‑150 (MQ=255)
                                                                                                      gctgGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCt                                               <  2:1632567/150‑1 (MQ=255)
                                                                                                         ggCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCa                                            >  2:1356698/1‑150 (MQ=255)
                                                                                                           cgcAGGTGTTTATGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACg                                          >  2:991738/1‑150 (MQ=255)
                                                                                                           cgcAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACg                                          >  2:952247/1‑150 (MQ=255)
                                                                                                             cAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCt                                        >  2:1504696/1‑150 (MQ=255)
                                                                                                              aGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtg                                       <  1:245461/150‑1 (MQ=255)
                                                                                                               ggTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCAAACAGGTGAAACGCTTCACGCtgg                                      >  2:29041/1‑150 (MQ=255)
                                                                                                               ggTGTGTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCGGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtgg                                      <  2:1700222/150‑1 (MQ=255)
                                                                                                                gtgtTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtggt                                     <  1:1163501/150‑1 (MQ=255)
                                                                                                                gtgtTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCAAACAGGTGAAACGCTTCACGCtggt                                     >  2:574799/1‑150 (MQ=255)
                                                                                                                   tttAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGc                                  >  1:947675/1‑150 (MQ=255)
                                                                                                                   tttAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGc                                  >  1:941523/1‑150 (MQ=255)
                                                                                                                      aaGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAATTTTGATTGAGTACAGCAATGTCAGACTATCAACAGTTTCAAGTACAGGTGACCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAAc                               <  1:861837/150‑1 (MQ=255)
                                                                                                                         gccTAATGACATCGGCGGCGGTATTTTCCGCCGCTGTGCTGATTTTTTATGGAGTACAGCAATTTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGa                            <  1:465139/148‑1 (MQ=255)
                                                                                                                         gACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGa                            <  1:458755/150‑1 (MQ=255)
                                                                                                                          aCTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGAt                           >  1:765129/1‑150 (MQ=255)
                                                                                                                            tAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGGGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATgg                         <  1:1358435/150‑1 (MQ=255)
                                                                                                                            tAATGACATCGGCGGCGGTATTTTACGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATgg                         >  1:1682863/1‑150 (MQ=255)
                                                                                                                             aaTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGc                        >  2:1249082/1‑150 (MQ=255)
                                                                                                                             aaTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGc                        <  1:587748/150‑1 (MQ=255)
                                                                                                                              aTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCa                       <  2:1396640/150‑1 (MQ=255)
                                                                                                                              aTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCa                       <  1:212226/150‑1 (MQ=255)
                                                                                                                                gACATCGGCGGCGCTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCaaa                     <  1:589173/150‑1 (MQ=255)
                                                                                                                                 ccATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATTTTTGAAGTACAGCTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAAc                    <  1:593954/149‑1 (MQ=255)
                                                                                                                                   aTCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCa                  >  1:197598/1‑150 (MQ=255)
                                                                                                                                    ccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGCCTATCAAATGTTTGAAGTACCGGGGAGCCAGGTTGCACCCCTTACCGAACAGGGGAAACGCTTCACGCTGGTGGCACCCGAAGGCAAACCAt                 >  1:1061811/4‑150 (MQ=255)
                                                                                                                                    ccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGATCCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCAt                 >  1:661904/4‑150 (MQ=255)
                                                                                                                                    ccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCaa                 >  1:604590/4‑149 (MQ=255)
                                                                                                                                       gcggcggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTAc              >  2:378356/1‑150 (MQ=255)
                                                                                                                                           cggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGACCAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGc          <  1:307569/150‑1 (MQ=255)
                                                                                                                                           cggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACATGTGCAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGc          >  1:890581/1‑150 (MQ=255)
                                                                                                                                            ggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCg         >  2:3825/1‑150 (MQ=255)
                                                                                                                                                  ttCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTAcc   <  2:1667850/150‑1 (MQ=255)
                                                                                                                                                   tCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCg  <  2:810291/150‑1 (MQ=255)
                                                                                                                                                   tCCGCCGCTGGGCAGTTTTTTGATTGAGTACAGCAAAGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGATCCCCTTACCGCACAGGTGAAACGCTTCACGCTGGTGGCCCCCGATGTCAAACCATTACCGGCGTTTACCg  >  2:675276/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCG  >  NC_000913/1885663‑1885959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: