Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,898,316 T→C 5.0% intergenic (+20/‑111) sdaA → / → yoaD L‑serine dehydratase 1/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,898,3160TC 5.0% 356.4 / 6.8 119intergenic (+20/‑111)sdaA/yoaDL‑serine dehydratase 1/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production
Reads supporting (aligned to +/- strand):  ref base T (60/53);  new base C (3/3);  total (63/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

Only 100 of 122 total aligned reads displayed.

CCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTATCGTCGT  >  NC_000913/1898167‑1898465
                                                                                                                                                     |                                                                                                                                                     
ccgcACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAGCGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGAGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCt                                                                                                                                                       >  2:435537/1‑150 (MQ=255)
ccgcACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCt                                                                                                                                                       >  2:666502/1‑150 (MQ=255)
     ccAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAAc                                                                                                                                                  >  1:426657/1‑150 (MQ=255)
         tGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGAt                                                                                                                                              <  1:1511056/150‑1 (MQ=255)
            aCCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATggg                                                                                                                                           <  2:312520/150‑1 (MQ=255)
              cgcgcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCg                                                                                                                                         <  2:309/150‑1 (MQ=255)
              cgcgcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCCGTTGCAGATGGGCg                                                                                                                                         <  2:1065601/150‑1 (MQ=255)
              cgcgcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCCGTTGCAGATGGGCg                                                                                                                                         <  2:968864/150‑1 (MQ=255)
                cgcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCCGTTGCAGATGGGCGag                                                                                                                                       <  2:275464/150‑2 (MQ=255)
                 gcgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAAt                                                                                                                                      <  2:1051345/150‑1 (MQ=255)
                  cgTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAACGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAAtt                                                                                                                                     <  2:792063/150‑1 (MQ=255)
                   gTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAAttt                                                                                                                                    <  1:748432/150‑1 (MQ=255)
                      tcGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTGTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTtata                                                                                                                                 >  2:1582248/1‑150 (MQ=255)
                      tcGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTtata                                                                                                                                 <  1:330611/150‑1 (MQ=255)
                       cGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGCGCGAATTTATAc                                                                                                                                >  2:448461/1‑150 (MQ=255)
                       cGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGAGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATAc                                                                                                                                >  1:275526/1‑150 (MQ=255)
                         cTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATAccc                                                                                                                              <  2:958740/150‑1 (MQ=255)
                         cTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATAccc                                                                                                                              >  1:546725/1‑150 (MQ=255)
                          tGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCg                                                                                                                             <  2:555191/150‑1 (MQ=255)
                               aaGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTtc                                                                                                                        >  2:1329584/1‑150 (MQ=255)
                                aGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTtct                                                                                                                       >  1:108310/1‑150 (MQ=255)
                                aGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTtct                                                                                                                       <  1:15335/150‑1 (MQ=255)
                                  gTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACTCCAAATACCGCGAAACCTCACGCGGTTGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCg                                                                                                                     <  1:553212/150‑1 (MQ=255)
                                  gTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCg                                                                                                                     <  1:1452466/150‑1 (MQ=255)
                                  gTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCg                                                                                                                     <  2:507037/150‑1 (MQ=255)
                                    cATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTc                                                                                                                   <  1:1387285/150‑1 (MQ=255)
                                      tCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTctg                                                                                                                 <  2:1624957/150‑1 (MQ=255)
                                      gcGAATCGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACAAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTctg                                                                                                                 <  2:68346/149‑1 (MQ=255)
                                       cGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTctgc                                                                                                                >  2:870112/1‑150 (MQ=255)
                                        gAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTctgct                                                                                                               >  1:1347599/1‑150 (MQ=255)
                                               tGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACACGCTTTCTCGTCTGCTGTAatat                                                                                                        >  1:1303187/1‑150 (MQ=255)
                                                 tACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTc                                                                                                      >  2:98270/1‑150 (MQ=255)
                                                 tACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTc                                                                                                      >  1:1352810/1‑150 (MQ=255)
                                                 tACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTc                                                                                                      >  1:1213579/1‑150 (MQ=255)
                                                   cGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATTTTccc                                                                                                    >  1:1531765/1‑150 (MQ=255)
                                                     aaaCCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCa                                                                                                  <  1:1090717/150‑1 (MQ=255)
                                                      aaCCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCAc                                                                                                 <  2:336964/150‑1 (MQ=255)
                                                       aCCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACt                                                                                                >  2:1694877/1‑150 (MQ=255)
                                                        ccGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTa                                                                                               <  1:796500/150‑1 (MQ=255)
                                                           gTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTacac                                                                                            >  1:198057/1‑150 (MQ=255)
                                                           gTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTacac                                                                                            >  1:375075/1‑150 (MQ=255)
                                                              aGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTc                                                                                         <  1:98270/150‑1 (MQ=255)
                                                               ggACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTcc                                                                                        >  1:1529248/1‑150 (MQ=255)
                                                                  cATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACt                                                                                     >  2:731974/1‑150 (MQ=255)
                                                                   aTGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTg                                                                                    <  2:879985/150‑1 (MQ=255)
                                                                   aTGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTg                                                                                    <  2:426657/150‑1 (MQ=255)
                                                                   aTGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTg                                                                                    >  1:1430194/1‑150 (MQ=255)
                                                                    tGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACCCTTCCACTGt                                                                                   >  2:576479/1‑150 (MQ=255)
                                                                     gAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGtt                                                                                  >  1:816357/1‑150 (MQ=255)
                                                                      aaCGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAAGATTCCCCACTACACTTCCACTGTTg                                                                                 >  2:291703/1‑150 (MQ=255)
                                                                        cGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTTCACTTCCACTGTTGCg                                                                               >  1:531810/1‑150 (MQ=255)
                                                                        cGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCg                                                                               >  1:1638360/1‑150 (MQ=255)
                                                                         gCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGt                                                                              >  2:413835/1‑150 (MQ=255)
                                                                             aaTACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAgg                                                                          >  1:632203/1‑150 (MQ=255)
                                                                             aaTACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAgg                                                                          <  2:1455051/150‑1 (MQ=255)
                                                                                aCCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGt                                                                       >  2:1701728/1‑150 (MQ=255)
                                                                                 ccGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGtt                                                                      >  2:935572/1‑150 (MQ=255)
                                                                                 ccGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGtt                                                                      >  2:1114717/1‑150 (MQ=255)
                                                                                   gcgAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTg                                                                    <  1:292270/150‑1 (MQ=255)
                                                                                   gcgAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTg                                                                    <  2:18022/150‑1 (MQ=255)
                                                                                   gcgAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTg                                                                    >  2:32294/1‑150 (MQ=255)
                                                                                     gAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTc                                                                  <  2:275526/150‑1 (MQ=255)
                                                                                      aaaCCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCg                                                                 >  2:577164/1‑150 (MQ=255)
                                                                                        aCCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGcc                                                               >  1:592016/1‑150 (MQ=255)
                                                                                        aCCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGcc                                                               <  2:889840/150‑1 (MQ=255)
                                                                                        aCCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGcc                                                               >  2:1711543/1‑150 (MQ=255)
                                                                                        aCCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGGCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGcc                                                               <  1:724921/150‑1 (MQ=255)
                                                                                          cTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCAt                                                             >  2:791612/1‑150 (MQ=255)
                                                                                            cACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTc                                                                  <  1:987699/143‑1 (MQ=255)
                                                                                            cACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATAc                                                           >  2:355174/1‑150 (MQ=255)
                                                                                              cgcgGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGc                                                         <  1:485524/150‑1 (MQ=255)
                                                                                                     gtCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAgg                                                  >  1:1025985/1‑150 (MQ=255)
                                                                                                        tGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTg                                               >  1:1620817/1‑150 (MQ=255)
                                                                                                            aaTCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGccc                                           <  1:591088/150‑1 (MQ=255)
                                                                                                            aaTCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCATACAGGGTGGccc                                           >  1:459753/1‑150 (MQ=255)
                                                                                                                aaaGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCAt                                       >  1:1060867/1‑150 (MQ=255)
                                                                                                                aaaGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCAt                                       >  2:1013255/1‑150 (MQ=255)
                                                                                                                      cAGTGTTACTAATACTTCTTACTCCCCCATCTGCAACGGATGGGCGAATTTATACCCGCCTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCaaaa                                 <  1:187980/150‑1 (MQ=255)
                                                                                                                            gACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTCCACTTCCACTGTTGCGTCCGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGcaca                           >  2:134786/1‑150 (MQ=255)
                                                                                                                            gACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGcaca                           >  2:1620984/1‑150 (MQ=255)
                                                                                                                              cTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAAc                         >  1:1074246/1‑150 (MQ=255)
                                                                                                                                aaTACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACgg                       <  1:731974/150‑1 (MQ=255)
                                                                                                                                aaTACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACgg                       <  2:1430194/150‑1 (MQ=255)
                                                                                                                                    cttcttACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCa                   <  2:632203/150‑1 (MQ=255)
                                                                                                                                    cttcttACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCa                   >  2:1594392/1‑150 (MQ=255)
                                                                                                                                      tcttACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTAGCCCGCATGCAAAAAGCACAACGGATCAtt                 <  2:1303187/150‑1 (MQ=255)
                                                                                                                                       cttACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGTGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTa                <  1:89287/150‑1 (MQ=255)
                                                                                                                                       cttACTCGCCCATCTGCAACGGATGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTaa               >  2:231814/1‑150 (MQ=255)
                                                                                                                                          attCGCCCATCCGTTGCAGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCTCTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTaaaa             >  2:250434/3‑150 (MQ=255)
                                                                                                                                          aCTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTaaaa             <  1:448461/150‑1 (MQ=255)
                                                                                                                                           ttCGCCCATCCGTTGCAGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc            >  1:1397404/2‑150 (MQ=255)
                                                                                                                                           ttCGCCCATCCGTTGCAGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc            >  2:1473073/2‑150 (MQ=255)
                                                                                                                                           cTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATTTTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc            <  2:1531765/150‑1 (MQ=255)
                                                                                                                                           cTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAAc            >  1:303632/1‑150 (MQ=255)
                                                                                                                                             cGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCt          >  1:1557059/1‑150 (MQ=255)
                                                                                                                                               cccATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTAt        >  1:278225/1‑150 (MQ=255)
                                                                                                                                               cccATCTGCAACGGATGGGCGAATTTATACCCGCTGTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGGTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAACAGAACAACGGATCATTAAATCCTAt        >  1:1227240/1‑150 (MQ=255)
                                                                                                                                                   tCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTAtcgtc    <  1:355174/150‑1 (MQ=255)
                                                                                                                                                   tCTGCAACGGATGGGCGAATTTATACCAGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTAtcgtc    <  1:355176/150‑1 (MQ=255)
                                                                                                                                                     tGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTATcgtcgt  <  2:697940/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAACGATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTGACTAATACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACTTCCACTGTTGCGTCAGGCGTTTGTCGCCATACGCTTACAGGGTGGCCCGCATGCAAAAAGCACAACGGATCATTAAAACCTATCGTCGT  >  NC_000913/1898167‑1898465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: