Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,900,672 G→A 8.5% T305M (ACG→ATG) ‡ yoaE ← putative membrane protein/conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,900,6720GA 8.5% 209.6 / 4.8 70T305M (ACG→ATG) ‡yoaEputative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (29/35);  new base A (3/3);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCC  >  NC_000913/1900526‑1900818
                                                                                                                                                  |                                                                                                                                                  
gcagTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGAGGATTTCATCTACCCCTAGATTAGCGTCAACCCAGCTTAATTCACCACGCGGCGTCa                                                                                                                                                 >  2:1630345/1‑150 (MQ=255)
 cagTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCAt                                                                                                                                                <  2:586264/150‑1 (MQ=255)
      cTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATcc                                                                                                                                           >  2:123109/1‑150 (MQ=255)
          aGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCcgcg                                                                                                                                       <  1:331670/150‑1 (MQ=255)
           gCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGTCGTCATGATCCCgcgc                                                                                                                                      >  1:95345/1‑150 (MQ=255)
           gCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCgcgc                                                                                                                                      <  1:395341/150‑1 (MQ=255)
              cGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCaga                                                                                                                                   <  1:445535/150‑1 (MQ=255)
               gTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCagag                                                                                                                                  <  2:797580/150‑1 (MQ=255)
                tACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGGATCATGACGCCgcgcggtga                                                                                                                                 <  1:1248457/150‑6 (MQ=255)
                tACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGGATCATGACGCCgcgcggtga                                                                                                                                 <  2:601519/150‑6 (MQ=255)
                 aCAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGGATCATGACGCCgcgcggt                                                                                                                                   >  1:1087027/1‑144 (MQ=255)
                 aCAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGGATCATGACGCCgcgcggt                                                                                                                                   <  2:1087027/147‑4 (MQ=255)
                    aTACCGATGATTTCATCCAGATCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAAcgc                                                                                                                             <  1:461438/150‑1 (MQ=255)
                        cGATGATATCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGAGAAGAGAGCACTAGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCAAGCATAATACACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGAcc                                                                                                                         >  1:59624/1‑149 (MQ=255)
                              tttCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCg                                                                                                                   >  2:1251160/1‑150 (MQ=255)
                                tCATCCTGTTCACCGCGACAAACCGGGAACAGACTGAGCGGTGTAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTc                                                                                                                 <  2:1469472/150‑1 (MQ=255)
                                 cATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCa                                                                                                                >  2:1683141/1‑150 (MQ=255)
                                   tCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGt                                                                                                              >  1:363618/1‑150 (MQ=255)
                                      aGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACg                                                                                                           >  2:1145014/1‑150 (MQ=255)
                                      aGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACg                                                                                                           <  1:1420020/150‑1 (MQ=255)
                                            ccGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTa                                                                                                     <  2:1243657/150‑1 (MQ=255)
                                            ccGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTa                                                                                                     <  2:23456/150‑1 (MQ=255)
                                                gACATACCGGGAACCGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATATCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCa                                                                                                 <  1:299194/150‑1 (MQ=255)
                                                  cATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATg                                                                                               >  1:934852/1‑150 (MQ=255)
                                                   aTACCGGGAACAGACTTTGCGGTGAAGAGAGCTGTTGCTCGCGGATTTCATCGACCCCGAGATTATAGTCAACCCAGCTTATTTCACCGCGCGTCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGt                                                                                              <  1:1630345/150‑1 (MQ=255)
                                                     aCCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTaa                                                                                            <  1:123109/150‑1 (MQ=255)
                                                      ccGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAAc                                                                                           >  2:1691794/1‑150 (MQ=255)
                                                         ggAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGtt                                                                                        <  2:445518/150‑1 (MQ=255)
                                                          gAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCACCCCAGCTTATTTCACCCCGCGGCGTCATGATCCACCGCAGAGAACGCGACGCCCGCGTCAGTACGCCGTTAATCAAGTAACGttc                                                                                       >  2:886225/1‑150 (MQ=255)
                                                          gAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGttc                                                                                       <  2:363618/150‑1 (MQ=255)
                                                           aaCAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATACCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGttct                                                                                      <  1:211790/150‑1 (MQ=255)
                                                           aaCAGACTGAGCTGTGAAGTGATCATTTTGTCGTGGATTTGATAGACCCCGAGATTAGCGTGAACGCTTCTTATTTCTCAGCGCGGAGTAATGAGCCCTCGCAGAGAAAGCGACTCGAGCGTCTGTACGCCGTTAATCATGTAACGttct                                                                                      <  1:292453/150‑1 (MQ=255)
                                                            aCAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGttctt                                                                                     >  1:1714310/1‑150 (MQ=255)
                                                             cAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGttcttc                                                                                    >  1:1266412/1‑150 (MQ=255)
                                                               gACTGTCCGGTGAAGAGAGCAGATGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTAATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACACCATCGTAAGTACGCCGTTAATCATGTAACGttcttctt                                                                                  >  1:657786/1‑150 (MQ=255)
                                                                 cTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCg                                                                                >  1:473018/1‑150 (MQ=255)
                                                                 cTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCg                                                                                <  2:384437/150‑1 (MQ=255)
                                                                 cTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCg                                                                                <  2:1485117/150‑1 (MQ=255)
                                                                      cGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCaaa                                                                           >  1:435437/1‑150 (MQ=255)
                                                                        ttGAAGAGAGCAGTTGCTCGAGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAAAGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACTTTCTTCTTCGGCAAATg                                                                         <  2:1094991/149‑1 (MQ=255)
                                                                          gAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCa                                                                       <  1:1683141/150‑1 (MQ=255)
                                                                            cgcgagCAGTTGCGCGCGGAGTTCAGCGACCCCGAGATTAGCGTCCACCCAGCTTATTTCACCGCGCGGCGTCATGAGCCCGCGCAGAGAACGCGACGCCAGCGTCAGTCCGCCGGTAATCAGGTAACGGTCTTCTGCGGCAAAGGCAcc                                                                     >  1:895766/4‑150 (MQ=255)
                                                                                 gCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCg                                                                <  2:502021/150‑1 (MQ=255)
                                                                                  cAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCgg                                                               <  2:857446/150‑1 (MQ=255)
                                                                                          cgcgGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTACTCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGatctgca                                                       >  2:102123/1‑150 (MQ=255)
                                                                                            cgGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGatcggcatc                                                     >  2:410152/1‑150 (MQ=255)
                                                                                              gATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGatcggcatcgg                                                   >  2:760320/1‑150 (MQ=255)
                                                                                              gATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGatcggcatcgg                                                   >  1:1029965/1‑150 (MQ=255)
                                                                                               aTTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCAGGatcggcatcggc                                                  >  1:1170546/1‑150 (MQ=255)
                                                                                                  tCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCggcatcggcatc                                               >  2:1325823/1‑150 (MQ=255)
                                                                                                     tCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCggg                                            <  1:754122/150‑1 (MQ=255)
                                                                                                      cGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGt                                           <  2:650932/150‑1 (MQ=255)
                                                                                                        aCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTa                                         <  2:1266412/150‑1 (MQ=255)
                                                                                                          cccGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATc                                       <  2:1376441/150‑1 (MQ=255)
                                                                                                          cccGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATc                                       <  2:405667/150‑1 (MQ=255)
                                                                                                            cGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCgg                                     <  2:1029965/150‑1 (MQ=255)
                                                                                                                aTTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATc                                 >  1:775051/1‑150 (MQ=255)
                                                                                                                 ttAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCg                                <  2:934852/150‑1 (MQ=255)
                                                                                                                  tAGCTTCAACCCAGCGTATTTCCCCGGGCGGCGTAATGATCCCGCGCCGAGAACGCGACGCCAGCGGCAGTACTCCGTTAATCATGTAACGTTCTTCTTCGTCAAATGCACCTTCCGTTAGCGGCATCGGCATCGGGTTATCGGAATCGt                               <  1:88567/150‑1 (MQ=255)
                                                                                                                     cGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCt                            <  2:435437/150‑1 (MQ=255)
                                                                                                                      gTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTg                           >  2:213159/1‑150 (MQ=255)
                                                                                                                               gCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGc                  >  2:437779/1‑150 (MQ=255)
                                                                                                                                    tttCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCCCCTTCCGGGATCGGCCTCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGAc             >  1:230672/1‑150 (MQ=255)
                                                                                                                                    tttCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGAc             <  1:1691794/150‑1 (MQ=255)
                                                                                                                                      tctctgcgcgGGATCATGACGCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCGTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGt           >  1:558916/6‑150 (MQ=255)
                                                                                                                                      tCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGt           <  1:1145014/150‑1 (MQ=255)
                                                                                                                                      tCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCATCGGCAAATGCACCTTACGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGt           <  1:170238/150‑1 (MQ=255)
                                                                                                                                        aCCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCAGCGGGGTATCGGGATCGTGCTGAACATTGGCCTGACGttt         >  1:102426/1‑150 (MQ=255)
                                                                                                                                         ctgcgcgGGATCATGACGCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTc        >  1:1584967/3‑150 (MQ=255)
                                                                                                                                            cgcgGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCg     >  2:1141173/1‑150 (MQ=255)
                                                                                                                                               ggcgTCATGATCCCGCGCAGAGAACGCGCCGCCAGCGTCAGGACGCCGTTAATCATGTAACGGTCTTCTTCGGCACATGCACCTTCCGGGATCGGCATCGGCATCGGGTGATCGGCATCGTGCTGAACATTGGCCTGACGTTgcccgccc  <  1:267642/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGATCGGCATCGGCATCGGGTTATCGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCC  >  NC_000913/1900526‑1900818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑