Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,906,229 T→G 6.2% I181S (ATC→AGC) ‡ mntP → putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,906,2290TG 6.2% 327.2 / 9.2 108I181S (ATC→AGC) ‡mntPputative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base T (59/42);  new base G (2/5);  total (61/47)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 108 total aligned reads displayed.

ACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGC  >  NC_000913/1906082‑1906376
                                                                                                                                                   |                                                                                                                                                   
aCATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATcc                                                                                                                                                   >  1:709486/1‑150 (MQ=255)
 cATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCct                                                                                                                                                  >  1:1409430/1‑150 (MQ=255)
  aTTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTTATCGGCATCGGCGTCCAGATCctc                                                                                                                                                 >  2:692592/1‑150 (MQ=255)
  aTTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                 <  2:363212/150‑1 (MQ=255)
  aTTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                 >  2:384089/1‑150 (MQ=255)
     aTCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTgg                                                                                                                                              <  1:1244927/150‑1 (MQ=255)
     aTCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGGGCTGATCGGCATCGGCGTCCAGATCCTCTgg                                                                                                                                              <  1:479467/150‑1 (MQ=255)
      tCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGa                                                                                                                                             <  1:1469398/150‑1 (MQ=255)
       cgcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGAc                                                                                                                                            >  1:607386/1‑150 (MQ=255)
        gcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACg                                                                                                                                           <  1:1296212/150‑1 (MQ=255)
        gcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCGTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACg                                                                                                                                           <  2:818345/150‑1 (MQ=255)
         cgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACGc                                                                                                                                          <  1:1568418/150‑1 (MQ=255)
          gACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCa                                                                                                                                         <  1:780832/150‑1 (MQ=255)
          gACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCa                                                                                                                                         <  2:76936/150‑1 (MQ=255)
          caCCGCATTGGCCATTGGGTGGGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGGCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAACTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACGcc                                                                                                                                         <  1:272964/149‑2 (MQ=255)
            ccGCAGTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGATCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACGccga                                                                                                                                       <  2:824094/150‑4 (MQ=255)
              gCATTGGCCATTGGTTGTGCACCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGCCGCACTTc                                                                                                                                     >  2:925657/1‑150 (MQ=255)
              gCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTc                                                                                                                                     <  2:1441327/150‑1 (MQ=255)
              gCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTc                                                                                                                                     >  1:1246049/1‑150 (MQ=255)
                  tGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACg                                                                                                                                 <  2:683365/150‑1 (MQ=255)
                  tGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACg                                                                                                                                 <  2:832650/150‑1 (MQ=255)
                   ggCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACgg                                                                                                                                >  2:126631/1‑150 (MQ=255)
                    gCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGt                                                                                                                               >  1:13592/1‑150 (MQ=255)
                      cATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGGCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTta                                                                                                                             <  1:1233258/150‑1 (MQ=255)
                        ttGGTTGTGCAACCTTGATTATGTCAACATTAGGGTTGATGGTTGTGCGCTTTATCGGCTAAATTATTGGTAAAAAATCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaat                                                                                                                           <  2:836800/150‑1 (MQ=255)
                         tGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaata                                                                                                                          <  1:104463/150‑1 (MQ=255)
                              gtgCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGTGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCATCTGTACGCACTTACACGGTTAATAAGAAc                                                                                                                     >  1:239355/1‑150 (MQ=255)
                               tgCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTACACGGTTTATAAGAACg                                                                                                                    >  2:426793/1‑150 (MQ=255)
                                gCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGc                                                                                                                   >  2:789571/1‑150 (MQ=255)
                                   aCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCATCTGGACGCACTACCCCGGTTAATAAGAACGCTGc                                                                                                                >  2:1229729/1‑150 (MQ=255)
                                      ttGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCag                                                                                                             >  2:1193558/1‑150 (MQ=255)
                                      ttGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCag                                                                                                             <  2:433378/150‑1 (MQ=255)
                                             tGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGCTCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                      >  2:1127247/1‑150 (MQ=255)
                                              gTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCAGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAat                                                                                                     >  2:198885/1‑150 (MQ=255)
                                               tCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTTCTGATCGGCATCGGCGTCCAGATCCTCTGGCCGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAatt                                                                                                    >  1:1233799/1‑149 (MQ=255)
                                               tCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGCTGGTGCTGATCGGCATCGGCGTCCAGCTCCTCTGGCCGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAatat                                                                                                   >  1:689491/1‑150 (MQ=255)
                                               tCAACATTAGGGATGATGGTTGGTAGATTTATCGGATCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATAGGCGTCAAGATCAAATGGAAGCAATGCCCCGGTTAATAAGAACGATGCCAGAGGTGAata                                                                                                    >  2:442847/1‑150 (MQ=255)
                                                cAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAatat                                                                                                   >  1:1234769/1‑150 (MQ=255)
                                                 aaCATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATAtt                                                                                                  >  1:24693/1‑150 (MQ=255)
                                                  aCATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTa                                                                                                 <  1:1229729/150‑1 (MQ=255)
                                                   cATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaa                                                                                                >  2:1413407/1‑150 (MQ=255)
                                                     ttAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGCAAAAACGCGTAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAg                                                                                              <  2:1670095/150‑1 (MQ=255)
                                                      tAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCGCTGGCCGCACTTCCACGGTTAATAAGAACGGTGCCAGAGGAGAATATTATAGt                                                                                             >  1:1539437/1‑150 (MQ=255)
                                                       tgggATGATGGTGGGGCGCTTTATCGGCTCAATTATTGGGAAATAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGGGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTc                                                                                            <  1:675093/149‑1 (MQ=255)
                                                       aGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCTGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTc                                                                                            <  2:331733/150‑1 (MQ=255)
                                                       aGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTc                                                                                            <  2:13592/150‑1 (MQ=255)
                                                         ggATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCg                                                                                          >  1:1073286/1‑150 (MQ=255)
                                                         ggATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCg                                                                                          >  2:631192/1‑150 (MQ=255)
                                                           atgatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTc                                                                                        <  1:991727/150‑1 (MQ=255)
                                                                   tggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTc                                                                                >  1:1475492/1‑150 (MQ=255)
                                                                       cGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAac                                                                            >  2:1666131/1‑150 (MQ=255)
                                                                        gCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAaca                                                                           >  1:238359/1‑150 (MQ=255)
                                                                         cTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAacac                                                                          <  1:694364/150‑1 (MQ=255)
                                                                            tATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCATCTGGACGCACTTACACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACActtc                                                                       >  1:307794/1‑150 (MQ=255)
                                                                              tCGGCTCAATTATTGGGATAAAAGCGGATTTTCTCGGCTGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGTTTAATAAGAACGCTGCCAGAGGTGAATATTATAGTCCGTCTGGCAGTCGATCActtctt                                                                     <  1:289474/150‑1 (MQ=255)
                                                                                ggCTCAATTATTGGGAAAAAAGCGCAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCtttt                                                                   <  1:846463/150‑1 (MQ=255)
                                                                                 gCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCAGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtg                                                                  >  1:490233/1‑150 (MQ=255)
                                                                                  gtCAATTATTGTGAAAAAATCGGAAATTCTCTGCGGGATGGTGCTGATCGTCATCGGAGTAAAGATCCTCTGGAAGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgc                                                                 <  2:209519/149‑1 (MQ=255)
                                                                                    cAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgctg                                                               >  2:414921/1‑150 (MQ=255)
                                                                                     aaatattGGGAAAAAAGCGGAAATTCTCGGCGGGCTGTTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgctgc                                                              <  1:40898/147‑1 (MQ=255)
                                                                                      attattGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGcc                                                             >  1:892701/1‑150 (MQ=255)
                                                                                      attattGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTCCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGcc                                                             >  1:1473946/1‑150 (MQ=255)
                                                                                           tGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAgtgt                                                        >  2:170435/1‑150 (MQ=255)
                                                                                                aaaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGcc                                                   <  1:789571/150‑1 (MQ=255)
                                                                                                  aaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAg                                                 <  1:1621103/150‑1 (MQ=255)
                                                                                                    aGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGAc                                               >  1:1248946/1‑150 (MQ=255)
                                                                                                     gCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCTAGATACTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACt                                              >  2:441202/1‑150 (MQ=255)
                                                                                                       ggAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTc                                            >  1:813324/1‑150 (MQ=255)
                                                                                                       ggAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTc                                            >  2:846329/1‑150 (MQ=255)
                                                                                                       ggAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCAGGACGCACTTCCACGGTTAATAAGAAAGCTGCCAGAGGTGAATATTAAAGTCACTCTGGCAGTCCAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTc                                            >  2:937807/1‑150 (MQ=255)
                                                                                                        gAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCt                                           >  2:1016321/1‑150 (MQ=255)
                                                                                                        gAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCt                                           <  1:384089/150‑1 (MQ=255)
                                                                                                           aTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGc                                        <  1:429658/150‑1 (MQ=255)
                                                                                                            ttagcggcggGCGGGTGCTGATCGGCTTCGGCGTCCATAGCCTCTGTAGGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCt                                       <  2:1409430/146‑1 (MQ=255)
                                                                                                            ttCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCt                                       >  2:42528/1‑150 (MQ=255)
                                                                                                              ctcGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTc                                     >  2:1306344/1‑150 (MQ=255)
                                                                                                               tcGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGACAGACTTCTGGCTTCg                                    >  2:388161/1‑150 (MQ=255)
                                                                                                                cggcggGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGc                                   >  2:253920/1‑150 (MQ=255)
                                                                                                                cggcggGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGc                                   >  2:1502539/1‑150 (MQ=255)
                                                                                                                   cggGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACg                                >  2:1470492/1‑150 (MQ=255)
                                                                                                                   cggGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACg                                <  1:414921/150‑1 (MQ=255)
                                                                                                                      gCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGccc                             >  2:1423042/1‑149 (MQ=255)
                                                                                                                      gCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCa                             >  2:607647/1‑150 (MQ=255)
                                                                                                                         ggggCTGAGCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGc                          <  2:1044724/147‑1 (MQ=255)
                                                                                                                         ggTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGc                          >  1:76615/1‑150 (MQ=255)
                                                                                                                           tGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCaa                        >  2:620970/1‑150 (MQ=255)
                                                                                                                           tGCTGATCAGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCaa                        <  1:198885/150‑1 (MQ=255)
                                                                                                                            gcggATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCaaa                       <  2:490163/147‑1 (MQ=255)
                                                                                                                            gCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCaaa                       >  2:550224/1‑150 (MQ=255)
                                                                                                                                  cggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGGCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGt                 <  1:148857/150‑1 (MQ=255)
                                                                                                                                   ggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTc                >  1:1541534/1‑150 (MQ=255)
                                                                                                                                    gcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCa               >  2:1585298/1‑150 (MQ=255)
                                                                                                                                     catcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACATCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCAt              <  1:692592/150‑1 (MQ=255)
                                                                                                                                        cggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTg           <  2:641720/150‑1 (MQ=255)
                                                                                                                                         tgcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAACGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCCCGCCAGGCCAACGGCGTCATCTGc          >  1:1704448/2‑150 (MQ=255)
                                                                                                                                         ggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc          >  1:1054927/1‑150 (MQ=255)
                                                                                                                                          gcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGACGAGAATATTAAAGTCCGTCAGGCAGTCGAACACTTCTTTTGCTGCCAGTCTTAGCCAGACTTCTGGCTTCGCACGCCACGCAAACGGCGACAGCTCCa         >  2:1446764/1‑150 (MQ=255)
                                                                                                                                            gTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaat       <  2:778566/150‑1 (MQ=255)
                                                                                                                                             tCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGCCTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaata      >  2:1157924/1‑150 (MQ=255)
                                                                                                                                                aGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATg   <  2:607386/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGC  >  NC_000913/1906082‑1906376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: