Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,912,750 T→A 8.3% intergenic (‑174/+18) htpX ← / ← prc putative endopeptidase/carboxy‑terminal protease for penicillin‑binding protein 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,912,7500TA 8.3% 321.5 / 11.2 115intergenic (‑174/+18)htpX/prcputative endopeptidase/carboxy‑terminal protease for penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base T (53/52);  new base A (6/4);  total (59/56)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

AAAACGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTT  >  NC_000913/1912602‑1912899
                                                                                                                                                    |                                                                                                                                                     
aaaaCGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTc                                                                                                                                                      >  2:1153437/1‑150 (MQ=255)
  aaCGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTGTATTCTGGTTAGTGATTAAGTCACCAGAAAGACACATCTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCtt                                                                                                                                                    <  1:49649/150‑1 (MQ=255)
     gCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTtgtg                                                                                                                                                 <  2:1365764/150‑1 (MQ=255)
       aaTATGCGATACCCACATCGTATGGGTTACTCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACGAGAAAGTCACTACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGcc                                                                                                                                               <  1:82977/150‑1 (MQ=255)
       aaTATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGcc                                                                                                                                               <  2:557374/150‑1 (MQ=255)
       aaTATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTGGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGcc                                                                                                                                               <  2:498383/150‑1 (MQ=255)
       aaTATGCGATACACACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGcc                                                                                                                                               <  1:708836/150‑1 (MQ=255)
        atatGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCt                                                                                                                                              <  1:1227725/150‑1 (MQ=255)
        atatGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCt                                                                                                                                              >  2:270966/1‑150 (MQ=255)
         tatGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAAGAAATTGTGCCtg                                                                                                                                             <  2:787264/150‑1 (MQ=255)
         tatGAGATACCCACATCGGATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCACAAAGACACACCTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCAGTTAAAAAATCAGGCACAAGAAATTGTGCCtg                                                                                                                                             <  2:278884/150‑1 (MQ=255)
           tGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCtgat                                                                                                                                           <  1:550581/150‑1 (MQ=255)
           tGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCtgat                                                                                                                                           <  2:1175269/150‑1 (MQ=255)
            gCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCGGATTTACGGCTGCTGTTCGATGTTAAAAAATCAGGCACAATTAATTGTGCCtgatt                                                                                                                                          >  1:1719161/1‑150 (MQ=255)
               aTACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGACAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCATGTCGCGGGTAAAAAATCAGGCACAATTTCTTGTGCCtgattttt                                                                                                                                       <  2:283488/150‑4 (MQ=255)
                 aCCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAAGAAATTGTGCCtgatttttta                                                                                                                                     <  1:1117462/150‑6 (MQ=255)
                  cccACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATAtt                                                                                                                                    >  1:104749/1‑150 (MQ=255)
                  cccACATCGAATGGGTTACGCGACTATATTCAAGTCTGGATACTGCGTAAGTCACCAGAAAGACACAAGTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCAGTTAAAAAATCAGGCACAATTTCTTGTGCCTGAATGATAtt                                                                                                                                    >  2:1207527/1‑150 (MQ=255)
                   ccACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGGCACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTCCCTGATTGATATTa                                                                                                                                   >  2:337789/1‑150 (MQ=255)
                   ccACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTa                                                                                                                                   >  1:927640/1‑150 (MQ=255)
                     acaTCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACt                                                                                                                                 <  1:1068102/150‑1 (MQ=255)
                          gTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACg                                                                                                                            <  1:613403/150‑1 (MQ=255)
                          gTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTATGCCTGATTGATATTACTTGACg                                                                                                                            <  2:658731/150‑1 (MQ=255)
                            aTGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACATTTTCTTGTGCCTGATTGATATTACTTGACggg                                                                                                                          >  2:1499670/1‑150 (MQ=255)
                            aTGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACggg                                                                                                                          >  1:786852/1‑150 (MQ=255)
                            aTGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACggg                                                                                                                          <  1:855693/150‑1 (MQ=255)
                               ggTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGc                                                                                                                       >  1:1125233/1‑150 (MQ=255)
                                        aCTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTc                                                                                                              >  1:1117190/1‑150 (MQ=255)
                                        aCTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTc                                                                                                              >  1:1122923/1‑150 (MQ=255)
                                        aCTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGAATCTGATTTACGGAATCATGTCGCTGTTAAAAAATCAGGCACAATTTATTGTGCCTGATTGATATTACTTGACGGGAGAGGGTTGTTc                                                                                                              >  1:1547907/1‑150 (MQ=255)
                                          tATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCcg                                                                                                            <  2:1082082/150‑1 (MQ=255)
                                                   gTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTgg                                                                                                   >  2:745762/1‑150 (MQ=255)
                                                     cTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTTATTGATATTACTTGACGGGAGAGGGTTGTTCCGCGGGTCTGGCt                                                                                                 >  2:331166/1‑150 (MQ=255)
                                                      tGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTATGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCtt                                                                                                <  1:998709/150‑1 (MQ=255)
                                                          tAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTc                                                                                            >  1:751106/1‑150 (MQ=255)
                                                           aGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGATCGGGTTGTTCCGCGGGTCTGGCTTTTTCa                                                                                           >  1:852341/1‑150 (MQ=255)
                                                            gTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCaa                                                                                          >  2:522461/1‑150 (MQ=255)
                                                            gTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCaa                                                                                          >  2:489235/1‑150 (MQ=255)
                                                             tGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAg                                                                                         >  1:32392/1‑150 (MQ=255)
                                                              gCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTATCATCTGATTTACTGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGTTTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGc                                                                                        <  1:957628/150‑1 (MQ=255)
                                                                     tCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCa                                                                                 <  1:517435/150‑1 (MQ=255)
                                                                      cACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAg                                                                                >  2:1261405/1‑150 (MQ=255)
                                                                      cACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAg                                                                                >  1:692/1‑150 (MQ=255)
                                                                       aCCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGa                                                                               >  1:760788/1‑150 (MQ=255)
                                                                        ccAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGAt                                                                              <  2:186084/150‑1 (MQ=255)
                                                                        ccAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGAt                                                                              <  1:1334196/150‑1 (MQ=255)
                                                                        ccAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGAt                                                                              <  2:35951/150‑1 (MQ=255)
                                                                           gAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGa                                                                           >  2:705184/1‑150 (MQ=255)
                                                                           gAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGAGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGa                                                                           >  1:413858/1‑150 (MQ=255)
                                                                              aGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGCCGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTg                                                                        >  1:1532155/1‑150 (MQ=255)
                                                                                 cacaACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGa                                                                     >  1:1368535/1‑150 (MQ=255)
                                                                                 cacaACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGa                                                                     >  1:970691/1‑150 (MQ=255)
                                                                                  acaACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGat                                                                    <  1:268840/150‑1 (MQ=255)
                                                                                   caACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTATTGTGCCTGATAGATATTACTAGACGGGAGAGGGTTGATAATCGGTACTGTCTTTTTAAAGCATCGCCAGATCGAGTGCGata                                                                   >  1:33885/1‑150 (MQ=255)
                                                                                         tACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCAcc                                                             >  2:1122416/1‑150 (MQ=255)
                                                                                           cATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGt                                                           >  2:821980/1‑150 (MQ=255)
                                                                                             ttttGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGtct                                                         <  2:1117190/150‑1 (MQ=255)
                                                                                             ttttGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGtct                                                         <  2:1122923/150‑1 (MQ=255)
                                                                                             ttttGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGtct                                                         <  2:1258484/150‑1 (MQ=255)
                                                                                                tGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCAt                                                      >  1:1442112/1‑150 (MQ=255)
                                                                                                 gTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATc                                                     >  1:468584/1‑150 (MQ=255)
                                                                                                   aGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCa                                                   >  2:696871/1‑150 (MQ=255)
                                                                                                    gCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAg                                                  >  2:589111/1‑150 (MQ=255)
                                                                                                      aTCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGAt                                                >  1:527658/1‑150 (MQ=255)
                                                                                                        ttGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATaa                                              <  1:181585/149‑1 (MQ=255)
                                                                                                        cTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATaa                                              <  1:1718448/150‑1 (MQ=255)
                                                                                                         tGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAg                                             >  1:1205432/1‑150 (MQ=255)
                                                                                                         tGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAg                                             >  1:1635405/1‑150 (MQ=255)
                                                                                                         tGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAg                                             <  2:413858/150‑1 (MQ=255)
                                                                                                         tGATTTACGGCATCTTGTCGCTGTTAAAAAATCACGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAg                                             <  2:734452/150‑1 (MQ=255)
                                                                                                           aTTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGa                                           <  1:1257669/150‑1 (MQ=255)
                                                                                                              tACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATcc                                        >  2:428480/1‑150 (MQ=255)
                                                                                                              tACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATcc                                        >  2:1189986/1‑150 (MQ=255)
                                                                                                               tcGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCg                                       <  1:1328687/149‑1 (MQ=255)
                                                                                                                cGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGTGTTGTTACGCGGGTCTGGCTTTTTCCAGCTTCGCCAGATCGAGTGCGATATTAACCGTCTCATCCAGATAAGGATCCgg                                      >  2:235271/1‑150 (MQ=255)
                                                                                                                cGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCgg                                      >  2:782236/1‑150 (MQ=255)
                                                                                                                            gCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATc                          <  2:1125233/150‑1 (MQ=255)
                                                                                                                            gCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATc                          <  1:522461/150‑1 (MQ=255)
                                                                                                                            gCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATc                          <  2:1542503/150‑1 (MQ=255)
                                                                                                                             cTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCt                         <  1:782236/150‑1 (MQ=255)
                                                                                                                               gTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCttt                       >  1:940238/1‑150 (MQ=255)
                                                                                                                                 tAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCg                     <  2:927640/150‑1 (MQ=255)
                                                                                                                                  aaaaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCgg                    >  1:1118099/1‑150 (MQ=255)
                                                                                                                                   aaaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGt                   >  2:110497/1‑150 (MQ=255)
                                                                                                                                   aaaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGt                   <  1:493641/150‑1 (MQ=255)
                                                                                                                                      aaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGa                <  1:696871/150‑1 (MQ=255)
                                                                                                                                       aTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGat               >  1:1647975/1‑150 (MQ=255)
                                                                                                                                       aTCAGGCACAAGAAATTGTGCCTGATTGATATTACTTGAAGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCGCCTGGTAATCTTTCGGTTGat               >  1:854551/1‑150 (MQ=255)
                                                                                                                                         cAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatca             <  2:991911/150‑1 (MQ=255)
                                                                                                                                         cAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatca             >  1:1635479/1‑150 (MQ=255)
                                                                                                                                         cAGGCACAAGAAATTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatca             >  2:306481/1‑150 (MQ=255)
                                                                                                                                         cAGGCACAAGAAATTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatca             >  1:1577157/1‑150 (MQ=255)
                                                                                                                                         cAGGCACAAGAAATTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTATCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATACGGCTCCTGGTAATCTTTCGGTAGatca             >  1:4285/1‑150 (MQ=255)
                                                                                                                                           ggCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatcatc           >  2:864796/1‑150 (MQ=255)
                                                                                                                                            gCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATcc          >  2:493561/1‑150 (MQ=255)
                                                                                                                                            gCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATcc          <  2:1293364/150‑1 (MQ=255)
                                                                                                                                                aaTTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAATCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGtt      <  1:1441818/150‑1 (MQ=255)
                                                                                                                                                 aTTTCTTGTGCCTGATTGAAATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGTTCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGttt     <  2:1547907/150‑1 (MQ=255)
                                                                                                                                                    tCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCGGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTttctt  <  2:1579742/150‑1 (MQ=255)
                                                                                                                                                    tCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTttctt  <  2:1719161/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAAACGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTT  >  NC_000913/1912602‑1912899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: