Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,972,797 A→T 5.3% intergenic (‑106/+39) tar ← / ← cheW methyl‑accepting chemotaxis protein II/purine‑binding chemotaxis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,972,7970AT 5.3% 280.8 / 3.7 94intergenic (‑106/+39)tar/cheWmethyl‑accepting chemotaxis protein II/purine‑binding chemotaxis protein
Reads supporting (aligned to +/- strand):  ref base A (44/45);  new base T (2/3);  total (46/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

AGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCCCAGTCCAG  >  NC_000913/1972648‑1972945
                                                                                                                                                     |                                                                                                                                                    
aGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCa                                                                                                                                                      >  2:837108/1‑150 (MQ=255)
 gCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAg                                                                                                                                                     >  2:1022450/1‑150 (MQ=255)
    ccATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGatc                                                                                                                                                  >  2:704451/1‑150 (MQ=255)
        tACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCg                                                                                                                                              <  1:464466/150‑1 (MQ=255)
         aCCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCgg                                                                                                                                             >  1:512583/1‑150 (MQ=255)
         aCCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGGTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCgg                                                                                                                                             >  1:1013917/1‑150 (MQ=255)
            aaCAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGtt                                                                                                                                          <  1:1587678/150‑1 (MQ=255)
                gCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCAtt                                                                                                                                      >  1:1337667/1‑150 (MQ=255)
                gCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGATCTGCGCATCGGGtttttt                                                                                                                                      <  1:646898/150‑5 (MQ=255)
                  gTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTc                                                                                                                                    <  1:704451/150‑1 (MQ=255)
                   tGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCa                                                                                                                                   <  2:544369/150‑1 (MQ=255)
                   tGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCa                                                                                                                                   <  2:551007/150‑1 (MQ=255)
                   tGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGATCTGCGCATCGGGttttttatt                                                                                                                                   <  2:1183950/150‑8 (MQ=255)
                      cTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCaagt                                                                                                                                >  1:839715/1‑148 (MQ=255)
                      cTACGCGGATACGGTTAATCATAAGGCACATTCATGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAAtt                                                                                                                                >  1:759535/1‑150 (MQ=255)
                          gctgATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAgg                                                                                                                            <  1:1337587/147‑1 (MQ=255)
                               tACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATc                                                                                                                       <  1:1516838/150‑1 (MQ=255)
                                aCGGTTAATCATAAGGCACCTTCCTGAAAACAAGGTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCg                                                                                                                      <  2:247055/150‑1 (MQ=255)
                                 cGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGTGCGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCgg                                                                                                                     <  1:137633/150‑1 (MQ=255)
                                  ggTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCggg                                                                                                                    <  1:423432/150‑1 (MQ=255)
                                  ggTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGAAGATCATCGGGTTCATTTCAATTGAGGAAATCggg                                                                                                                    >  2:706107/1‑150 (MQ=255)
                                      aaTCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGaa                                                                                                                >  2:1635300/1‑150 (MQ=255)
                                      aaTCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCAGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGaa                                                                                                                >  1:914258/1‑150 (MQ=255)
                                        tCATTAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGTGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAAtt                                                                                                              <  2:355826/150‑1 (MQ=255)
                                            aaGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGc                                                                                                          <  2:1337667/150‑1 (MQ=255)
                                             aGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGcc                                                                                                         >  2:937685/1‑150 (MQ=255)
                                              ggCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCa                                                                                                        >  1:1483377/1‑150 (MQ=255)
                                                    ttCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTg                                                                                                  <  2:759535/150‑1 (MQ=255)
                                                        tGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgc                                                                                              >  2:399501/1‑150 (MQ=255)
                                                         gAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGCTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCAGAcgcc                                                                                             >  2:346696/1‑150 (MQ=255)
                                                                 gTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATc                                                                                     <  1:837108/150‑1 (MQ=255)
                                                                  ttGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCt                                                                                    >  1:1072718/1‑150 (MQ=255)
                                                                      tctcGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACa                                                                                <  1:1696786/150‑1 (MQ=255)
                                                                      tctcGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCAACCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATAGCGTTCATTTCAATAGAGGAAAACGGGAGAAATACGCCACTTCTTACGACGAGCTAACTAACa                                                                                >  1:1447062/1‑150 (MQ=255)
                                                                        tcGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAgc                                                                              <  1:922403/150‑1 (MQ=255)
                                                                        tcGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAgc                                                                              >  2:280723/1‑150 (MQ=255)
                                                                           ttATCGGCAATGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGcc                                                                           <  2:1246734/150‑1 (MQ=255)
                                                                           ttATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGcc                                                                           <  2:1353953/150‑1 (MQ=255)
                                                                            tATCGGCAAGGAGGGGGGAAACATTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCa                                                                          <  1:972509/150‑1 (MQ=255)
                                                                                  cAAGTAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCtt                                                                    <  1:706107/150‑1 (MQ=255)
                                                                                  cAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCAGATCCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTTACGCCGCGCTATCTAACAGCGCCATCTCtt                                                                    >  2:327194/1‑150 (MQ=255)
                                                                                   aaGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTc                                                                   <  1:1635300/150‑1 (MQ=255)
                                                                                         gggggAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGtt                                                             >  2:641336/1‑150 (MQ=255)
                                                                                              aaaCTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTcagca                                                        >  2:29635/1‑150 (MQ=255)
                                                                                              aaaCTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTAACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTcagca                                                        >  1:625029/1‑150 (MQ=255)
                                                                                                aCTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGt                                                      >  1:19348/1‑150 (MQ=255)
                                                                                                aCTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGAGAAGATCATCGGGTTCATGTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTGAGCAGt                                                      >  1:13361/1‑150 (MQ=255)
                                                                                                 cTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGtt                                                     >  2:217921/1‑150 (MQ=255)
                                                                                                  tttATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGttt                                                    >  1:1541981/1‑150 (MQ=255)
                                                                                                     aTTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGTAATCGGGAGAATTACGCCTCTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGtttttg                                                 <  1:346696/150‑2 (MQ=255)
                                                                                                      ttGCTGATGCCACCCGCCGCGAAATTGAAATAATAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGATATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCg                                                <  2:1602783/150‑1 (MQ=255)
                                                                                                         cTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATg                                             >  1:951716/1‑150 (MQ=255)
                                                                                                         cTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCAGTTCAGCAGTTATTCGATg                                             >  1:1198129/1‑150 (MQ=255)
                                                                                                            aTGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTc                                          <  1:280723/150‑1 (MQ=255)
                                                                                                            aTGCCACCCGCCGCGAAAATGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTc                                          <  1:778008/150‑1 (MQ=255)
                                                                                                              ggcagccgcagCGAAATTGAAATAAAAAACCCGATGCTCAGATCATCGGGTTCATTTCAATTGAGGAAATCTTGAGAATTTCGCCACTTCTGACGACGCGCTATATAACAGTGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCAc                                        <  1:1148679/145‑1 (MQ=255)
                                                                                                                cACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGGAGTTTTTCGATGTTCACCa                                      >  2:818271/1‑150 (MQ=255)
                                                                                                                cACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCATCGCTGTTCAGCAGTTTTTCGATGTTCACCa                                      <  2:1509702/150‑1 (MQ=255)
                                                                                                                 aCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAg                                     <  2:1072718/150‑1 (MQ=255)
                                                                                                                 aCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAg                                     <  1:1209478/150‑1 (MQ=255)
                                                                                                                    cgccgcGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAAt                                  <  2:1013917/150‑1 (MQ=255)
                                                                                                                    cgccgcGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAAt                                  <  2:512583/150‑1 (MQ=255)
                                                                                                                      ccgcGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCa                                <  1:923061/150‑1 (MQ=255)
                                                                                                                      ccgcGAAATTGAAATAAAAAACCCGATGCGCAGATCAGCGGGTTCAGTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCGTCTCTTCGCTGTTCAGCAGTTGTTCGATGTTCCCCAGAGTCa                                >  1:1049672/1‑150 (MQ=255)
                                                                                                                       cgcgAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCaa                               <  1:1424484/150‑1 (MQ=255)
                                                                                                                        gcgAATTTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAAc                              <  1:865041/150‑1 (MQ=255)
                                                                                                                        gcgAAATTGAAATAAAAAACCCTATGCGCATCTCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTAGGCCTCTTCTGACGCCGCGCTTTCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCTAc                              <  2:1447062/150‑1 (MQ=255)
                                                                                                                         cgAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACa                             <  1:857855/150‑1 (MQ=255)
                                                                                                                            aaTTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATcc                          <  2:1678035/150‑1 (MQ=255)
                                                                                                                               tGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCTCTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGAGGTTCACCAGAATCAACATCCGGt                       <  1:1003520/150‑1 (MQ=255)
                                                                                                                                  aaTAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGc                    >  1:152083/1‑150 (MQ=255)
                                                                                                                                   gaaatgaaCCCGATGATCTGCGCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTc                      >  2:929642/7‑147 (MQ=255)
                                                                                                                                   gaaatgaaCCCGATGATCTGCGCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTc                      <  1:929642/141‑1 (MQ=255)
                                                                                                                                    tAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGccc                  >  1:220546/1‑150 (MQ=255)
                                                                                                                                    tAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGccc                  <  1:1645190/150‑1 (MQ=255)
                                                                                                                                     aaaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGcccc                 >  2:1494718/1‑149 (MQ=255)
                                                                                                                                     aaaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCa                 >  2:905159/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGATGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCAGGTCGCCCa                 >  2:1084369/1‑150 (MQ=255)
                                                                                                                                      aaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCat                <  2:13361/150‑2 (MQ=255)
                                                                                                                                      aaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAg                <  2:19348/150‑1 (MQ=255)
                                                                                                                                       tgaaCCCGATGATCTGCGCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGCCGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGt               >  1:836797/3‑150 (MQ=255)
                                                                                                                                       aaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGt               >  1:696133/1‑150 (MQ=255)
                                                                                                                                          aCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTgcg            >  2:1004070/1‑150 (MQ=255)
                                                                                                                                          aCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCAGCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTgcg            <  1:1022450/150‑1 (MQ=255)
                                                                                                                                           cccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACAACCGGTCGCCCAGTgcgc           >  2:1179163/1‑150 (MQ=255)
                                                                                                                                            ccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGcc          <  2:152083/150‑1 (MQ=255)
                                                                                                                                            ccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGcc          >  2:933559/1‑150 (MQ=255)
                                                                                                                                            ccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGcc          >  2:90010/1‑150 (MQ=255)
                                                                                                                                            ccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGcc          >  1:127564/1‑150 (MQ=255)
                                                                                                                                              gATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCcca        <  1:217921/150‑1 (MQ=255)
                                                                                                                                              gATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCcca        >  1:1367027/1‑150 (MQ=255)
                                                                                                                                                tGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCccagt      >  2:347048/1‑150 (MQ=255)
                                                                                                                                                  cgcAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCccagtcc    <  1:399501/150‑1 (MQ=255)
                                                                                                                                                    cAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCccagtccag  >  2:1247739/1‑150 (MQ=255)
                                                                                                                                                    cAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACAAGAATCAACATCCGGTCGCCCAGTGCGCccagtccag  >  1:317186/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCCCAGTCCAG  >  NC_000913/1972648‑1972945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: