Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,972,800 T→G 5.0% intergenic (‑109/+36) tar ← / ← cheW methyl‑accepting chemotaxis protein II/purine‑binding chemotaxis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,972,8000TG 5.0% 277.6 / 3.0 93intergenic (‑109/+36)tar/cheWmethyl‑accepting chemotaxis protein II/purine‑binding chemotaxis protein
Reads supporting (aligned to +/- strand):  ref base T (43/45);  new base G (2/3);  total (45/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCCCAGTCCAGTG  >  NC_000913/1972652‑1972947
                                                                                                                                                    |                                                                                                                                                   
ccATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGatc                                                                                                                                                    >  2:704451/1‑150 (MQ=255)
    tACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCg                                                                                                                                                <  1:464466/150‑1 (MQ=255)
     aCCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCgg                                                                                                                                               >  1:512583/1‑150 (MQ=255)
     aCCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGGTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCgg                                                                                                                                               >  1:1013917/1‑150 (MQ=255)
        aaCAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGtt                                                                                                                                            <  1:1587678/150‑1 (MQ=255)
            gCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCAtt                                                                                                                                        >  1:1337667/1‑150 (MQ=255)
            gCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGATCTGCGCATCGGGtttttt                                                                                                                                        <  1:646898/150‑5 (MQ=255)
              gTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTc                                                                                                                                      <  1:704451/150‑1 (MQ=255)
               tGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCa                                                                                                                                     <  2:544369/150‑1 (MQ=255)
               tGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCa                                                                                                                                     <  2:551007/150‑1 (MQ=255)
               tGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGATCTGCGCATCGGGttttttatt                                                                                                                                     <  2:1183950/150‑8 (MQ=255)
                  cTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCaagt                                                                                                                                  >  1:839715/1‑148 (MQ=255)
                  cTACGCGGATACGGTTAATCATAAGGCACATTCATGAAAACAAGTTGATCTCGTTATCGCCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAAtt                                                                                                                                  >  1:759535/1‑150 (MQ=255)
                      gctgATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAgg                                                                                                                              <  1:1337587/147‑1 (MQ=255)
                           tACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATc                                                                                                                         <  1:1516838/150‑1 (MQ=255)
                            aCGGTTAATCATAAGGCACCTTCCTGAAAACAAGGTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCg                                                                                                                        <  2:247055/150‑1 (MQ=255)
                             cGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGTGCGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCgg                                                                                                                       <  1:137633/150‑1 (MQ=255)
                              ggTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCggg                                                                                                                      <  1:423432/150‑1 (MQ=255)
                              ggTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGAAGATCATCGGGTTCATTTCAATTGAGGAAATCggg                                                                                                                      >  2:706107/1‑150 (MQ=255)
                                  aaTCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGaa                                                                                                                  >  2:1635300/1‑150 (MQ=255)
                                  aaTCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCAGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGaa                                                                                                                  >  1:914258/1‑150 (MQ=255)
                                    tCATTAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGTGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAAtt                                                                                                                <  2:355826/150‑1 (MQ=255)
                                        aaGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGc                                                                                                            <  2:1337667/150‑1 (MQ=255)
                                         aGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGcc                                                                                                           >  2:937685/1‑150 (MQ=255)
                                          ggCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCa                                                                                                          >  1:1483377/1‑150 (MQ=255)
                                                ttCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTg                                                                                                    <  2:759535/150‑1 (MQ=255)
                                                    tGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgc                                                                                                >  2:399501/1‑150 (MQ=255)
                                                     gAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGCTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCAGAcgcc                                                                                               >  2:346696/1‑150 (MQ=255)
                                                             gTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATc                                                                                       <  1:837108/150‑1 (MQ=255)
                                                              ttGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCt                                                                                      >  1:1072718/1‑150 (MQ=255)
                                                                  tctcGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACa                                                                                  <  1:1696786/150‑1 (MQ=255)
                                                                  tctcGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCAACCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATAGCGTTCATTTCAATAGAGGAAAACGGGAGAAATACGCCACTTCTTACGACGAGCTAACTAACa                                                                                  >  1:1447062/1‑150 (MQ=255)
                                                                    tcGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAgc                                                                                >  2:280723/1‑150 (MQ=255)
                                                                    tcGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAgc                                                                                <  1:922403/150‑1 (MQ=255)
                                                                       ttATCGGCAATGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGcc                                                                             <  2:1246734/150‑1 (MQ=255)
                                                                       ttATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGcc                                                                             <  2:1353953/150‑1 (MQ=255)
                                                                        tATCGGCAAGGAGGGGGGAAACATTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCa                                                                            <  1:972509/150‑1 (MQ=255)
                                                                              cAAGTAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCtt                                                                      <  1:706107/150‑1 (MQ=255)
                                                                              cAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCAGATCCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTTACGCCGCGCTATCTAACAGCGCCATCTCtt                                                                      >  2:327194/1‑150 (MQ=255)
                                                                               aaGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTc                                                                     <  1:1635300/150‑1 (MQ=255)
                                                                                     gggggAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGtt                                                               >  2:641336/1‑150 (MQ=255)
                                                                                          aaaCTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTcagca                                                          >  2:29635/1‑150 (MQ=255)
                                                                                          aaaCTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTAACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTcagca                                                          >  1:625029/1‑150 (MQ=255)
                                                                                            aCTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGt                                                        >  1:19348/1‑150 (MQ=255)
                                                                                            aCTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGAGAAGATCATCGGGTTCATGTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTGAGCAGt                                                        >  1:13361/1‑150 (MQ=255)
                                                                                             cTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGtt                                                       >  2:217921/1‑150 (MQ=255)
                                                                                              tttATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGttt                                                      >  1:1541981/1‑150 (MQ=255)
                                                                                                 aTTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGTAATCGGGAGAATTACGCCTCTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGtttttg                                                   <  1:346696/150‑2 (MQ=255)
                                                                                                  ttGCTGATGCCACCCGCCGCGAAATTGAAATAATAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGATATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCg                                                  <  2:1602783/150‑1 (MQ=255)
                                                                                                     cTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATg                                               >  1:951716/1‑150 (MQ=255)
                                                                                                     cTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCAGTTCAGCAGTTATTCGATg                                               >  1:1198129/1‑150 (MQ=255)
                                                                                                        aTGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTc                                            <  1:280723/150‑1 (MQ=255)
                                                                                                        aTGCCACCCGCCGCGAAAATGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTc                                            <  1:778008/150‑1 (MQ=255)
                                                                                                          ggcagccgcagCGAAATTGAAATAAAAAACCCGATGCTCAGATCATCGGGTTCATTTCAATTGAGGAAATCTTGAGAATTTCGCCACTTCTGACGACGCGCTATATAACAGTGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCAc                                          <  1:1148679/145‑1 (MQ=255)
                                                                                                            cACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGGAGTTTTTCGATGTTCACCa                                        >  2:818271/1‑150 (MQ=255)
                                                                                                            cACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCATCGCTGTTCAGCAGTTTTTCGATGTTCACCa                                        <  2:1509702/150‑1 (MQ=255)
                                                                                                             aCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAg                                       <  2:1072718/150‑1 (MQ=255)
                                                                                                             aCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAg                                       <  1:1209478/150‑1 (MQ=255)
                                                                                                                cgccgcGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAAt                                    <  2:1013917/150‑1 (MQ=255)
                                                                                                                cgccgcGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAAt                                    <  2:512583/150‑1 (MQ=255)
                                                                                                                  ccgcGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCa                                  <  1:923061/150‑1 (MQ=255)
                                                                                                                  ccgcGAAATTGAAATAAAAAACCCGATGCGCAGATCAGCGGGTTCAGTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCGTCTCTTCGCTGTTCAGCAGTTGTTCGATGTTCCCCAGAGTCa                                  >  1:1049672/1‑150 (MQ=255)
                                                                                                                   cgcgAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCaa                                 <  1:1424484/150‑1 (MQ=255)
                                                                                                                    gcgAATTTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAAc                                <  1:865041/150‑1 (MQ=255)
                                                                                                                    gcgAAATTGAAATAAAAAACCCTATGCGCATCTCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTAGGCCTCTTCTGACGCCGCGCTTTCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCTAc                                <  2:1447062/150‑1 (MQ=255)
                                                                                                                     cgAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACa                               <  1:857855/150‑1 (MQ=255)
                                                                                                                        aaTTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATcc                            <  2:1678035/150‑1 (MQ=255)
                                                                                                                           tGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCTCTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGAGGTTCACCAGAATCAACATCCGGt                         <  1:1003520/150‑1 (MQ=255)
                                                                                                                              aaTAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGc                      >  1:152083/1‑150 (MQ=255)
                                                                                                                               gaaatgaaCCCGATGATCTGCGCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTc                        <  1:929642/141‑1 (MQ=255)
                                                                                                                               gaaatgaaCCCGATGATCTGCGCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTc                        >  2:929642/7‑147 (MQ=255)
                                                                                                                                tAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGccc                    >  1:220546/1‑150 (MQ=255)
                                                                                                                                tAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGccc                    <  1:1645190/150‑1 (MQ=255)
                                                                                                                                 aaaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGcccc                   >  2:1494718/1‑149 (MQ=255)
                                                                                                                                 aaaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCa                   >  2:905159/1‑150 (MQ=255)
                                                                                                                                 aaaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGATGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCAGGTCGCCCa                   >  2:1084369/1‑150 (MQ=255)
                                                                                                                                  aaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCat                  <  2:13361/150‑2 (MQ=255)
                                                                                                                                  aaaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAg                  <  2:19348/150‑1 (MQ=255)
                                                                                                                                   tgaaCCCGATGATCTGCGCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGCCGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGt                 >  1:836797/3‑150 (MQ=255)
                                                                                                                                   aaaaCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGt                 >  1:696133/1‑150 (MQ=255)
                                                                                                                                      aCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTgcg              >  2:1004070/1‑150 (MQ=255)
                                                                                                                                      aCCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCAGCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTgcg              <  1:1022450/150‑1 (MQ=255)
                                                                                                                                       cccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACAACCGGTCGCCCAGTgcgc             >  2:1179163/1‑150 (MQ=255)
                                                                                                                                        ccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGcc            <  2:152083/150‑1 (MQ=255)
                                                                                                                                        ccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGcc            >  2:90010/1‑150 (MQ=255)
                                                                                                                                        ccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGcc            >  1:127564/1‑150 (MQ=255)
                                                                                                                                        ccGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGcc            >  2:933559/1‑150 (MQ=255)
                                                                                                                                          gATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCcca          <  1:217921/150‑1 (MQ=255)
                                                                                                                                          gATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCcca          >  1:1367027/1‑150 (MQ=255)
                                                                                                                                            tGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCccagt        >  2:347048/1‑150 (MQ=255)
                                                                                                                                              cgcAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCccagtcc      <  1:399501/150‑1 (MQ=255)
                                                                                                                                                cAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCccagtccag    >  2:1247739/1‑150 (MQ=255)
                                                                                                                                                cAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACAAGAATCAACATCCGGTCGCCCAGTGCGCccagtccag    >  1:317186/1‑150 (MQ=255)
                                                                                                                                                  gATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCCAAGTCCAGTg  >  1:1426257/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAAGGAGGGGGGAAACTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAGCGCCATCTCTTCGCTGTTCAGCAGTTTTTCGATGTTCACCAGAATCAACATCCGGTCGCCCAGTGCGCCCAGTCCAGTG  >  NC_000913/1972652‑1972947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: