Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,006,465 A→T 6.1% N104Y (AAC→TAC)  amyA → cytoplasmic alpha‑amylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,006,4650AT 6.1% 266.3 / 5.7 98N104Y (AAC→TAC) amyAcytoplasmic alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base A (40/52);  new base T (2/4);  total (42/56)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

Only 100 of 101 total aligned reads displayed.

TCGGCTACGACTCCTATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTT  >  NC_000913/2006316‑2006614
                                                                                                                                                     |                                                                                                                                                     
tcggCTACGACTCCTATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCa                                                                                                                                                       >  1:490099/1‑150 (MQ=255)
        gACTCCTATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTTGACTACcacatc                                                                                                                                               <  1:750637/150‑3 (MQ=255)
        gACTCCTATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTTGACTACcacatc                                                                                                                                               <  1:44383/150‑3 (MQ=255)
        gACTCCTATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTTGACTACcacatc                                                                                                                                               <  1:1654089/150‑3 (MQ=255)
          cTCCTATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTTGACTACcacatcca                                                                                                                                             <  2:1696788/150‑5 (MQ=255)
          atCCTATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAAt                                                                                                                                             <  2:1116850/149‑1 (MQ=255)
            ccTATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATgg                                                                                                                                           >  1:1152917/1‑150 (MQ=255)
              tATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGgc                                                                                                                                         >  2:1550549/1‑150 (MQ=255)
              tATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGgc                                                                                                                                         <  2:1060938/150‑1 (MQ=255)
                tGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGgcgc                                                                                                                                       <  2:1152917/150‑1 (MQ=255)
                 gATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGcgcg                                                                                                                                      >  1:938786/1‑150 (MQ=255)
                 gATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGcgcg                                                                                                                                      >  1:437141/1‑150 (MQ=255)
                     tatttGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGTTg                                                                                                                                  >  2:944599/1‑150 (MQ=255)
                     tatttGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATg                                                                                                                                  <  1:452975/150‑1 (MQ=255)
                      atttGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGa                                                                                                                                 >  2:1087332/1‑150 (MQ=255)
                      atttGATTTAGGCGAGTTTGATCAGAAAGGAATCATCCATACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGa                                                                                                                                 <  2:482777/150‑1 (MQ=255)
                       tttGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGaa                                                                                                                                <  2:364506/150‑1 (MQ=255)
                       tatGATTTAGGCGAGGTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGaa                                                                                                                                <  2:364508/148‑1 (MQ=255)
                        ttGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGaaa                                                                                                                               >  2:1601666/1‑150 (MQ=255)
                          taTTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGAAAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGGAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGaaaaa                                                                                                                             <  2:1088108/149‑1 (MQ=255)
                          gttttAGGCGTGTTTTATCAGAAAGGCATCATCCCTAATAAATATGGAGATAAAGCACAACTGCTGGCCGCAATTGATGCTCTGAAACGTAATGACATTGCTGTGCTGTTTGATGTTGTAGTCAACCACAAAATGGGCTCGGATGaaaaa                                                                                                                             <  2:102619/148‑1 (MQ=255)
                            tttAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTTCTGTCCGCCATTGATGCACTGAAACGTAATGACATTGCTGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAaaga                                                                                                                           <  1:213337/150‑1 (MQ=255)
                            tttAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAaaga                                                                                                                           <  2:53082/150‑1 (MQ=255)
                                ggCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCt                                                                                                                       <  2:1005019/150‑1 (MQ=255)
                                ggCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCt                                                                                                                       >  2:860266/1‑150 (MQ=255)
                                ggCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCt                                                                                                                       >  2:718943/1‑150 (MQ=255)
                                ggCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCt                                                                                                                       <  1:25554/150‑1 (MQ=255)
                                     gTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTcg                                                                                                                  >  2:1201503/1‑150 (MQ=255)
                                       ttGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTcgcg                                                                                                                >  2:434321/1‑150 (MQ=255)
                                       ttGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTcgcg                                                                                                                >  1:1159770/1‑150 (MQ=255)
                                        tGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGCTGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGt                                                                                                               >  2:866835/1‑150 (MQ=255)
                                         gATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTg                                                                                                              >  1:1279231/1‑150 (MQ=255)
                                         gATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTg                                                                                                              >  2:196513/1‑150 (MQ=255)
                                         gATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCAGGCCGCCATTGATCCTCTGAAACGTAATCACATTGCGGTGCAGTAGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGCAGCTATTCGCGTg                                                                                                              >  2:1277185/1‑150 (MQ=255)
                                          aTCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGc                                                                                                             >  2:122145/1‑150 (MQ=255)
                                            cAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAg                                                                                                           >  2:1686904/1‑150 (MQ=255)
                                                  ggCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTa                                                                                                     >  1:1282325/1‑150 (MQ=255)
                                                  ggCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTa                                                                                                     <  1:1087332/150‑1 (MQ=255)
                                                  ggCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTa                                                                                                     >  2:1581591/1‑150 (MQ=255)
                                                   gcagcaTCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTaa                                                                                                    >  2:1679361/1‑150 (MQ=255)
                                                        aTCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCt                                                                                               <  2:1489213/150‑1 (MQ=255)
                                                              actaAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGAc                                                                                         <  2:437141/150‑1 (MQ=255)
                                                              actaAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGAc                                                                                         <  2:938786/150‑1 (MQ=255)
                                                               ctaAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGAcc                                                                                        >  1:247123/1‑150 (MQ=255)
                                                                  aaTATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTa                                                                                     <  1:1552655/150‑1 (MQ=255)
                                                                  aaTATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTa                                                                                     <  2:1282325/150‑1 (MQ=255)
                                                                     atGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGGTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGc                                                                                  <  1:111408/150‑1 (MQ=255)
                                                                        gCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCaaa                                                                               >  2:589322/1‑150 (MQ=255)
                                                                           aTAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAAt                                                                              <  2:1688555/148‑1 (MQ=255)
                                                                           aTAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAAt                                                                              >  1:1688555/1‑148 (MQ=255)
                                                                            tAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGa                                                                           <  1:362858/150‑1 (MQ=255)
                                                                              aaGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACg                                                                         <  2:221232/150‑1 (MQ=255)
                                                                               aGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACga                                                                        <  1:1550549/150‑1 (MQ=255)
                                                                               aGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACga                                                                        >  1:1176915/1‑150 (MQ=255)
                                                                                 cacaACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACtaag                                                                      >  1:1037422/1‑150 (MQ=255)
                                                                                  acaACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACgaaga                                                                     <  1:1125120/150‑1 (MQ=255)
                                                                                  acaACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACgaaga                                                                     <  1:860266/150‑1 (MQ=255)
                                                                                  acaACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACgaaga                                                                     <  1:718943/150‑1 (MQ=255)
                                                                                   caACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACgaagaa                                                                    <  1:586090/150‑1 (MQ=255)
                                                                                     aCTGCTGGCCGCGATTGATGCTCTGTAACGTAATGACATTGCGGTGCTTTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACgaagaatt                                                                  <  1:1254393/150‑3 (MQ=255)
                                                                                     aCTGCTGGCCGCCATTGATGCTCTGAATCGTTATGAGATTGCGGTTCTGTTGGTTGTGGTAGGCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGGGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAAt                                                                  <  2:871459/150‑1 (MQ=255)
                                                                                      ctgctgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATc                                                                 <  2:707448/150‑1 (MQ=255)
                                                                                      ctgctgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTCCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATc                                                                 <  1:270412/150‑1 (MQ=255)
                                                                                        gctgGCCGCCATTGATGCTCTGAAACTTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGTATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCAt                                                               <  1:1101569/150‑1 (MQ=255)
                                                                                        gctgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCAt                                                               <  2:1121056/150‑1 (MQ=255)
                                                                                          tgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTg                                                             >  1:1676980/1‑150 (MQ=255)
                                                                                          tgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTg                                                             <  1:220913/150‑1 (MQ=255)
                                                                                             ccgccATTGATGCTCTGTTACGTAATGACATTGCGGAGCTGTTGGATGTGGTTGTCAAACACAAAATGGGCGCGTATGAAAAAGAAGCTATTCGCTTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGTAGAAATCATTGAgt                                                          <  1:1277185/150‑1 (MQ=255)
                                                                                              cgccATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAgtg                                                         <  2:228272/150‑1 (MQ=255)
                                                                                              cgccATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAgtg                                                         <  1:236740/150‑1 (MQ=255)
                                                                                              cgccATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGAGGTGTTAGGCACCCACAAAATGGGCGCGGACGAAAAAGAAGCTATTCGCGTGCAGCGGGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAgtg                                                         <  1:89141/150‑1 (MQ=255)
                                                                                               gccATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAgtgt                                                        >  1:284180/1‑150 (MQ=255)
                                                                                               gccATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAgtgt                                                        >  2:1006787/1‑150 (MQ=255)
                                                                                                  aTTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGaa                                                     <  2:238884/150‑1 (MQ=255)
                                                                                                   ttGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACTCATATTGACGAAGCAATCATTGAGTGTGAAg                                                    >  1:1054904/1‑150 (MQ=255)
                                                                                                    tGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAgg                                                   <  2:1461077/150‑1 (MQ=255)
                                                                                                           ctGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGAcg                                            >  2:1444130/1‑150 (MQ=255)
                                                                                                           ctGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGAcg                                            >  1:536449/1‑150 (MQ=255)
                                                                                                              aaaCGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGAGAAGGCTGGACGCGt                                         <  2:1176915/150‑1 (MQ=255)
                                                                                                                 cGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTac                                      <  1:196513/150‑1 (MQ=255)
                                                                                                                 cGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTac                                      >  2:52518/1‑150 (MQ=255)
                                                                                                                  gTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTacc                                     >  2:601551/1‑149 (MQ=255)
                                                                                                                        aCATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTcc                               <  2:827747/150‑1 (MQ=255)
                                                                                                                           ttGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCcccg                            <  2:247123/150‑1 (MQ=255)
                                                                                                                             gCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCcccgcc                          >  1:136290/1‑150 (MQ=255)
                                                                                                                                  gCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGc                     >  2:1480968/1‑150 (MQ=255)
                                                                                                                                    tGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCg                   <  2:1676980/150‑1 (MQ=255)
                                                                                                                                    cccattttgtgGTTGACTACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCg                   >  1:948153/8‑150 (MQ=255)
                                                                                                                                     gTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCgg                  <  2:564856/150‑1 (MQ=255)
                                                                                                                                     gTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCgg                  <  2:1360147/150‑1 (MQ=255)
                                                                                                                                       tGGATGTGGTAGTCTACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTCCGCATATTCACGAAGAATTCAATGAGTGTCAAGGCTGGACGCGTTACACATTCCCCGCCCGTGCCGGGc                >  1:561459/1‑150 (MQ=255)
                                                                                                                                         gATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCaa              <  1:1581591/150‑1 (MQ=255)
                                                                                                                                         gATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCaa              >  1:109771/1‑150 (MQ=255)
                                                                                                                                          aTGGGGTAGTCAACCACAAAATGGGCGCGGAGGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAAt             <  1:631590/150‑1 (MQ=255)
                                                                                                                                           tgtgGTAGACAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGAAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGTGTGTGAAGGCTGGACGCGTTACACATTCCCCGCCCGTGCCGGGCaaaa            >  2:1682278/1‑148 (MQ=255)
                                                                                                                                             tgGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTAAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACt          <  2:633252/150‑1 (MQ=255)
                                                                                                                                                 aGTCAACCACAGAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCa      >  2:892783/1‑150 (MQ=255)
                                                                                                                                                   tCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGt    <  1:1261905/150‑1 (MQ=255)
                                                                                                                                                     aaCCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCTTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCAGGGCAATACTCGCAGtat  >  1:140889/1‑148 (MQ=255)
                                                                                                                                                     aaCCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGttt  >  2:1056427/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGGCTACGACTCCTATGATTTATTTGATTTAGGCGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGCGATAAAGCACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTT  >  NC_000913/2006316‑2006614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: