Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,057,601 C→A 9.6% intergenic (+27/+426) yeeN → / ← asnW UPF0082 family protein/tRNA‑Asn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,057,6010CA 9.6% 292.6 / 19.4 114intergenic (+27/+426)yeeN/asnWUPF0082 family protein/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base C (52/50);  new base A (3/8);  total (56/58)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01

Only 100 of 114 total aligned reads displayed.

GATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTA  >  NC_000913/2057454‑2057749
                                                                                                                                                   |                                                                                                                                                     
gATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACgg                                                                                                                                                     >  1:1490527/1‑150 (MQ=255)
gATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACgg                                                                                                                                                     <  2:1603155/150‑1 (MQ=255)
    gCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGa                                                                                                                                                 <  2:191748/150‑1 (MQ=255)
       cAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAttt                                                                                                                                              >  2:1499892/1‑150 (MQ=255)
        aaTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTc                                                                                                                                             >  2:31659/1‑150 (MQ=255)
        aaTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTc                                                                                                                                             <  2:212612/150‑1 (MQ=255)
         aTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCt                                                                                                                                            <  1:53881/150‑1 (MQ=255)
          tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCtt                                                                                                                                           <  1:1014378/150‑1 (MQ=255)
          tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCtt                                                                                                                                           <  2:1219419/150‑1 (MQ=255)
          tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCtt                                                                                                                                           <  2:2411/150‑1 (MQ=255)
          tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCtt                                                                                                                                           <  1:1003290/150‑1 (MQ=255)
          tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATAATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCtt                                                                                                                                           <  2:1418878/150‑1 (MQ=255)
          tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGATTGTTGATGCAATTGAATATGACGAAGATTCAAAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGAGAGATTTCtt                                                                                                                                           <  1:83942/150‑1 (MQ=255)
           cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                                                                          >  2:698211/1‑150 (MQ=255)
           cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                                                                          <  2:1476734/150‑1 (MQ=255)
           cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                                                                          <  2:567556/150‑1 (MQ=255)
            tGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGAAGATTTCTTTa                                                                                                                                         >  2:185583/1‑150 (MQ=255)
            tGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTa                                                                                                                                         <  1:1387957/150‑1 (MQ=255)
                     gCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCatat                                                                                                                                <  2:1077933/150‑1 (MQ=255)
                         tCCCCAGAAGATATAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATATCTTAAATCTCATATAAtt                                                                                                                            >  1:276706/1‑150 (MQ=255)
                            ccAGAAGATTTAGAAATCTTTGAAGGGCTTGTTAACGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTc                                                                                                                           <  1:1423804/148‑1 (MQ=255)
                            ccAGAAGATTTAGAAATCTTTGAAGGGCTTGTTAAAGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTc                                                                                                                           >  2:1423804/1‑148 (MQ=255)
                             cAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTta                                                                                                                        <  1:1350897/150‑1 (MQ=255)
                                  gaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaa                                                                                                                   <  2:1223439/150‑1 (MQ=255)
                                    tttAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaaaa                                                                                                                 >  1:1229781/1‑150 (MQ=255)
                                      tAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTGCAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaata                                                                                                               >  1:316251/1‑150 (MQ=255)
                                        gAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATTACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGAAGATTACTTTAATCTAATATAATTCTTATAAAAaatata                                                                                                             >  1:106060/1‑150 (MQ=255)
                                        gAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatata                                                                                                             <  2:1555433/150‑1 (MQ=255)
                                        gAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatata                                                                                                             <  1:1041419/150‑1 (MQ=255)
                                          aaTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataat                                                                                                           >  1:727730/1‑150 (MQ=255)
                                              tttGAAGTGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTc                                                                                                       <  1:1004912/150‑1 (MQ=255)
                                              tttGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTc                                                                                                       <  1:1004915/150‑1 (MQ=255)
                                               ttGAAGGGCTTGTTGATGCACTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACTTCACAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCa                                                                                                      <  1:1668216/150‑1 (MQ=255)
                                               ttGAAGGGCTTGTTGATGCACTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCa                                                                                                      <  1:1189941/150‑1 (MQ=255)
                                                tGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCaa                                                                                                     >  1:1420658/1‑150 (MQ=255)
                                                 gtaGGGCTTGTTGATTGCCTTGAAGATGACGAAGATGTACAAAAAGTTTATAATATCGTCGCAAATCTCTAATTATCTTTTAATGAAATCTGTCTTTACGGCATATTTCTTTAATCTAATATATTTGTTATTAAAATTATAATATTCAAc                                                                                                    <  2:78062/148‑1 (MQ=255)
                                                 gAAGGGCTTTTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAc                                                                                                    <  2:1406260/150‑1 (MQ=255)
                                                   aGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTc                                                                                                  <  2:818604/150‑1 (MQ=255)
                                                    gggCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCg                                                                                                 <  1:31659/150‑1 (MQ=255)
                                                     ggCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTAAAATCGt                                                                                                >  2:1513490/1‑150 (MQ=255)
                                                        ttgttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCat                                                                                             <  1:795743/150‑1 (MQ=255)
                                                         tgttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCata                                                                                            >  1:1121805/1‑150 (MQ=255)
                                                            tgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTg                                                                                         >  1:145483/1‑150 (MQ=255)
                                                            tgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTAACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTg                                                                                         >  1:735189/1‑150 (MQ=255)
                                                             gATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGa                                                                                        >  2:1399216/1‑150 (MQ=255)
                                                              aTGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGAt                                                                                       <  2:97077/150‑1 (MQ=255)
                                                                  ccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATGATATTCAACTCGTCATATTGATtata                                                                                   <  2:814037/150‑1 (MQ=255)
                                                                   cTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtataa                                                                                  >  1:915190/1‑149 (MQ=255)
                                                                   cTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAc                                                                                 <  1:27339/150‑1 (MQ=255)
                                                                      gAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcccc                                                                              >  1:646659/1‑150 (MQ=255)
                                                                        ggATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                                                                               >  1:1313219/2‑132 (MQ=255)
                                                                        ggATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                                                                               <  2:1313219/131‑1 (MQ=255)
                                                                        aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                                                                               >  1:1509064/1‑132 (MQ=255)
                                                                        aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                                                                               <  2:1509064/132‑1 (MQ=255)
                                                                        aGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcccccc                                                                            >  1:658098/1‑150 (MQ=255)
                                                                         gATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcccccac                                                                           >  1:1281354/1‑148 (MQ=255)
                                                                              cgacgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTcc                                                                      >  1:800082/1‑150 (MQ=255)
                                                                               gacgaTGTACAAAAAGTTTATCATAAAGTCTCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGTCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTccc                                                                     <  2:250569/150‑1 (MQ=255)
                                                                                  gaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCaga                                                                  >  2:587082/1‑150 (MQ=255)
                                                                                   aTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCagag                                                                 >  2:1474047/1‑150 (MQ=255)
                                                                                      tACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGaaa                                                              <  1:790234/150‑1 (MQ=255)
                                                                                        cAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTATGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCACCCGTTCCCAGAGAaata                                                            >  2:1328165/1‑150 (MQ=255)
                                                                                         aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataa                                                           >  2:613619/1‑150 (MQ=255)
                                                                                         aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCAGTCTTTACGGCAGATTTCTTTAATCTCATATAATACTTAAAAAAAATATAATATTCAACTCGTCATATTGATTATACAAACACGTTCCAAGataaataa                                                           >  1:1252077/1‑150 (MQ=255)
                                                                                          aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAaataa                                                           >  2:1471481/1‑150 (MQ=255)
                                                                                              gTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtattt                                                      <  1:698211/150‑1 (MQ=255)
                                                                                                  aTCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATttatta                                                  >  2:391938/1‑150 (MQ=255)
                                                                                                     aTAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAAACCCCCGTTACCAGAGAAATAATATTTATTaaaa                                               >  2:115920/1‑150 (MQ=255)
                                                                                                          gTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCa                                          >  1:1710884/1‑150 (MQ=255)
                                                                                                                aaTCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTACAGttctt                                    >  2:477001/1‑150 (MQ=255)
                                                                                                                  tctcTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTtcttct                                  >  2:780306/1‑150 (MQ=255)
                                                                                                                   ctctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTcttctt                                 <  2:1121805/150‑1 (MQ=255)
                                                                                                                     ctAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                               >  1:1383901/1‑150 (MQ=255)
                                                                                                                       aaTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTc                             <  2:1321857/150‑1 (MQ=255)
                                                                                                                         ttATCTTTTAAAGAAATCTCTCTTAACGGCAGATTTCTTTAATATCATATATTTCTTATAAAAAATATAATATTCAACTCGTCATTTTGATTATACCCCCCCGTTCCCAGAGAAAATATATTTATTAAAATACCATTTCTTCGTTTTCtt                           >  2:1193672/1‑149 (MQ=255)
                                                                                                                          tATCTTTTAAAGAAATATGTATTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGa                          <  1:185583/150‑1 (MQ=255)
                                                                                                                           aTCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAt                         <  2:1490527/150‑1 (MQ=255)
                                                                                                                           aTCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAt                         >  1:1545304/1‑150 (MQ=255)
                                                                                                                            tCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAtt                        <  1:221782/150‑1 (MQ=255)
                                                                                                                             cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa                       >  1:893355/1‑150 (MQ=255)
                                                                                                                             cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACGCACGTTCCCTGAGATATAATATTTATTTAATTTCCAGTTCTTCTTTTTCTGATTa                       >  1:307317/1‑150 (MQ=255)
                                                                                                                              ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc                      <  1:1499892/150‑1 (MQ=255)
                                                                                                                               tttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACa                     <  2:658098/150‑1 (MQ=255)
                                                                                                                               tttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACa                     >  2:1592890/1‑150 (MQ=255)
                                                                                                                               attAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAATAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCGAGTTCTTCTTTTTCTGATTACa                     >  1:34032/2‑150 (MQ=39)
                                                                                                                                 tAAAGAAATCTTTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa                   <  1:54910/150‑1 (MQ=255)
                                                                                                                                 tAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa                   >  2:686613/1‑150 (MQ=255)
                                                                                                                                 tAAAGAAATCTGTCCTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa                   >  1:548006/1‑150 (MQ=255)
                                                                                                                                 tAAAGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa                   >  2:102759/1‑150 (MQ=255)
                                                                                                                                 tAAAGAAATATGTCTTTACGTCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCTTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGa                   <  1:1513490/150‑1 (MQ=255)
                                                                                                                                  aaaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAAAAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa                  >  1:650407/1‑150 (MQ=255)
                                                                                                                                  aaaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTAATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCGCGTTCCCATAGAAATAATATTTATTAAAATTGCAGTTCTTCTTTTTCTGATTACAGaa                  <  2:276706/150‑1 (MQ=255)
                                                                                                                                    aGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTAAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCAAGTTCTTGTTTTTCTGATTACAGAAgg                >  1:947896/1‑150 (MQ=255)
                                                                                                                                    aGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGa                          >  2:910530/1‑140 (MQ=255)
                                                                                                                                    aGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGa                          <  1:910530/140‑1 (MQ=255)
                                                                                                                                      aaaTCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCa              <  2:316251/150‑1 (MQ=255)
                                                                                                                                      aaaTCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCa              >  2:1156809/1‑150 (MQ=255)
                                                                                                                                          cTGTCTTAACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGt          <  2:735189/150‑1 (MQ=255)
                                                                                                                                              cTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCa      >  2:1672595/1‑150 (MQ=255)
                                                                                                                                                  aCGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATAGAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGCGAAATAAGATTTATTAAAATTCAAGTTGTTCTTTTTATCATTACATAAGGAAAACTGGAAATTa  >  2:75606/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTA  >  NC_000913/2057454‑2057749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: