Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,074,691 C→G 6.5% intergenic (+33/‑88) flu → / → yeeR CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,6910CG 6.5% 335.2 / 10.6 117intergenic (+33/‑88)flu/yeeRCP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (52/55);  new base G (4/4);  total (58/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01

Only 100 of 123 total aligned reads displayed.

GAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCTT  >  NC_000913/2074542‑2074838
                                                                                                                                                      |                                                                                                                                                     
  aGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTcatcat                                                                                                                                                     >  2:899516/1‑150 (MQ=255)
    cccGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGa                                                                                                                                                   <  1:96318/150‑1 (MQ=255)
          gccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCGGGa                                                                                                                                             <  1:422497/149‑1 (MQ=255)
           ccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCGGGAc                                                                                                                                            <  1:1117767/150‑1 (MQ=255)
            cGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCGGGAcc                                                                                                                                           <  1:222546/150‑1 (MQ=255)
              ggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCg                                                                                                                                        >  2:452662/1‑150 (MQ=255)
              ggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCg                                                                                                                                        <  2:1533096/150‑1 (MQ=255)
                  aaTATCACCGTGGGCGGTCAGGCCGGTTATGCCCACTTCGCCTTCGGCAGCAGCGCTTAAGGTTATAACGTTCAGGCCACTCTGAATGTGACCTTCTGACAGAACCATGGCCGGTCTGTGGGCCCGGTCATCATCACCGGGACCCGGAcc                                                                                                                                    <  2:828219/150‑1 (MQ=255)
                     atCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCGGCCCCgc                                                                                                                                 >  1:565369/1‑150 (MQ=255)
                        aCCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCa                                                                                                                              <  2:1025693/150‑1 (MQ=255)
                         cccTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCaa                                                                                                                             >  2:853631/1‑150 (MQ=255)
                             gggCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGa                                                                                                                         <  2:965171/150‑1 (MQ=255)
                             gggCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGa                                                                                                                         <  2:119262/150‑1 (MQ=255)
                              ggCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGAt                                                                                                                        >  2:1070772/1‑150 (MQ=255)
                               gCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGCTc                                                                                                                       >  2:545861/1‑150 (MQ=255)
                               gCGTGCAGGCCGGTTATGCCCACCGCGTCAGCGGCAGCCGCGCTGAAGGTTATAACGGTCAGGCCACACTCAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGCCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATc                                                                                                                       <  2:656677/150‑1 (MQ=255)
                                 gTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCtt                                                                                                                     >  2:633420/1‑150 (MQ=255)
                                 gTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCACCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCtt                                                                                                                     >  1:202516/1‑150 (MQ=255)
                                     aGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAAc                                                                                                                 <  2:480745/150‑1 (MQ=255)
                                      ggCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGCCCGGCCCCACCGGTCTTAAACg                                                                                                                >  1:1548845/1‑150 (MQ=255)
                                      ggCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACg                                                                                                                <  1:988842/150‑1 (MQ=255)
                                      ggCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACg                                                                                                                >  2:1581869/1‑150 (MQ=255)
                                       gCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGc                                                                                                               >  1:1115907/1‑150 (MQ=255)
                                        ccGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGGCAGCATGACCGGGACCCGGACCGGCGCCACGGATCTTCAACGcc                                                                                                              >  1:1252190/1‑150 (MQ=255)
                                         cGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCca                                                                                                             <  2:1625896/150‑1 (MQ=255)
                                           gTTATGCCCACAGCGCCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCcaca                                                                                                           <  1:349810/150‑1 (MQ=255)
                                              aTGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTc                                                                                                        <  1:466633/150‑1 (MQ=255)
                                                gCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGCCCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGCCCGGCGCAACGGATCTTCAACGCCCCATTCGc                                                                                                      >  2:1440758/1‑150 (MQ=255)
                                                gCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGCCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGc                                                                                                      >  1:1053491/1‑150 (MQ=255)
                                                 cccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCt                                                                                                     <  2:313895/150‑1 (MQ=255)
                                                 cccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCt                                                                                                     >  1:18045/1‑150 (MQ=255)
                                                  ccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCGGACCGGGGCAGCGGGTCTTCGACGGCACATTGGCTg                                                                                                    >  2:1588291/1‑150 (MQ=255)
                                                  ccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTg                                                                                                    >  2:1003936/1‑150 (MQ=255)
                                                  ccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCAGACAGAACCATCGCCACTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGAGCTTCAACGCCACATTCCCTg                                                                                                    >  1:1111610/1‑150 (MQ=255)
                                                   cacaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCCCATTCGCTgg                                                                                                   >  2:369819/1‑150 (MQ=255)
                                                    acaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGc                                                                                                  <  1:1039724/150‑1 (MQ=255)
                                                       gCGTCAGCGGCAGCAGCGCTTAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATTGCCTCTCTGTGGGCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCAtt                                                                                               <  1:774243/150‑1 (MQ=255)
                                                        cGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGATCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTa                                                                                              <  1:736290/150‑1 (MQ=255)
                                                          tCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACATAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAAc                                                                                            <  1:610219/150‑1 (MQ=255)
                                                          tCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCTACGGATCTTCAACGACACATTCGCTGGCATTaaa                                                                                            >  2:1165614/1‑149 (MQ=255)
                                                          tCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAAc                                                                                            >  2:1151596/1‑150 (MQ=255)
                                                            aGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACaa                                                                                          >  1:1223820/1‑150 (MQ=255)
                                                                gcagcaGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAAc                                                                                      <  1:37788/150‑1 (MQ=255)
                                                                    cagcGCTGAAGGTTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGa                                                                                  <  2:1596943/150‑1 (MQ=255)
                                                                       cgcTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGatat                                                                               >  1:1155709/1‑150 (MQ=255)
                                                                       cgcTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGatat                                                                               >  1:20668/1‑150 (MQ=255)
                                                                                ttataAGGGTCTGGCCACACTTAATGTTACCTTCTCACATAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACgg                                                                      <  2:565369/149‑1 (MQ=255)
                                                                                ttaaaagggTCAGCCCAAACTTATTGTGACCTTCTCACAGAACCATCGCCTCTATGTGGCCCCGGTCATCATCACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACgg                                                                      <  1:736255/143‑1 (MQ=255)
                                                                                 tataACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGa                                                                     <  1:975604/150‑1 (MQ=255)
                                                                                  ataACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCAGTGGTCCCGGTCATCATGACCGGGACCCGGACAGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGAAATTCATCACGGAg                                                                    >  2:1012293/1‑150 (MQ=255)
                                                                                   taACGGTCAGGCCACACTGAATGGGCCCTTCTGCCCGAACCTCCCCCTCTCGGTGGTCCCGGGCATCATGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGt                                                                   <  1:823499/150‑1 (MQ=255)
                                                                                    aaCGGTCAGGCCACACTGAATCTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGGCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTg                                                                  <  2:1115888/150‑1 (MQ=255)
                                                                                      cGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCCACGGATCTTCAACGCCACATTAGCTGGCATTAACAATTACATGATATTCATCCCGGCGTGAc                                                                >  1:518068/1‑150 (MQ=255)
                                                                                          cAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATg                                                            <  1:304956/150‑1 (MQ=255)
                                                                                               cacaCTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACa                                                       >  2:1175134/1‑150 (MQ=255)
                                                                                               cacaCTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACa                                                       <  1:1070772/150‑1 (MQ=255)
                                                                                               caaagttaaTGTGACCTTCTCACAGAACCATCGCCTCTCTGGGGCCCCGGTCATCTTGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACa                                                       <  1:630555/143‑1 (MQ=255)
                                                                                                 cacTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATAATGACCGGGACCCGGACCGGCGCAACGGATCTTAAACGCCACAATAGCTGGCATTAACAATAACAAGATATTAATCACGGAGTGACTAAGTTACAGa                                                     >  1:1514960/1‑150 (MQ=255)
                                                                                                     gAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGt                                                 >  2:1194495/1‑150 (MQ=255)
                                                                                                        tgttACCTTCTGACTGAACCATCGCCTCTCTGTGTGCCCGGTCATCATTACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCgg                                              <  1:853631/150‑1 (MQ=255)
                                                                                                         gtgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgc                                             >  2:835427/1‑150 (MQ=255)
                                                                                                          tgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcg                                            >  1:746126/1‑150 (MQ=255)
                                                                                                          tgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcg                                            >  2:1641928/1‑150 (MQ=255)
                                                                                                          tgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcg                                            <  2:1620637/150‑1 (MQ=255)
                                                                                                           gACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCCGGTAAACATGCCCGGGACCCGACCCGGCCCAACGGTTATTCACCCCCCCATTCCCGGCCATTAACAATAACATAATATTCAACACGGAGGGACTATGTTAAAGATAGACGgcc                                            >  2:1631713/1‑149 (MQ=255)
                                                                                                           gACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgc                                           <  2:72824/150‑1 (MQ=255)
                                                                                                            aCCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATGTTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcg                                          <  2:18045/150‑1 (MQ=255)
                                                                                                              cTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCt                                        <  1:899516/150‑1 (MQ=255)
                                                                                                              cTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACAGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCt                                        >  1:1200470/1‑150 (MQ=255)
                                                                                                               ttCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTg                                       <  1:1175134/150‑1 (MQ=255)
                                                                                                                  tGACAGAACCATCGCGTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATc                                    <  2:411799/150‑1 (MQ=255)
                                                                                                                   gACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATcc                                   >  1:899750/1‑150 (MQ=255)
                                                                                                                    aCAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACGTTCGCTGGCATTAACAATAACATGATATTCATCACGGATTGTCTATGTTTCAGATATTCGGCGCGCTGATCct                                  >  2:444262/1‑150 (MQ=255)
                                                                                                                    aCAGAACCATCGCCTCTCTGTGGTCCCGGCCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCct                                  <  2:1053491/150‑1 (MQ=255)
                                                                                                                     cAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctg                                 >  1:237880/1‑150 (MQ=255)
                                                                                                                     cAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctg                                 >  1:986191/1‑150 (MQ=255)
                                                                                                                     aaGAACCATCGCCTCTCTGTGTCCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctg                                 <  2:1332102/149‑1 (MQ=255)
                                                                                                                       gAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgct                               <  2:758015/150‑1 (MQ=255)
                                                                                                                        aaCCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCTGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAAGAATAACATGATATTCCTCACGGAGTGACTATGTTACAGATAGTCGTCGCGCTGATCctgctg                              <  1:255391/150‑1 (MQ=255)
                                                                                                                          ccATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGAt                            <  2:900/150‑1 (MQ=255)
                                                                                                                          ccATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGAt                            >  2:200105/1‑150 (MQ=255)
                                                                                                                           cATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATc                           >  1:1027855/1‑150 (MQ=255)
                                                                                                                            aTCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCg                         <  2:1712479/150‑1 (MQ=255)
                                                                                                                                ccgctctGTGGTCCCGGTAATCATGACGGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAgg                     <  1:1099959/147‑1 (MQ=255)
                                                                                                                                ccTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAgg                     <  2:1115907/150‑1 (MQ=255)
                                                                                                                                 ctctctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGa                    >  1:474491/1‑150 (MQ=255)
                                                                                                                                 ctctctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGa                    >  2:1155818/1‑150 (MQ=255)
                                                                                                                                   ctctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCGATCAGCAGGAtc                  <  2:202516/150‑2 (MQ=255)
                                                                                                                                      tgtgGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTgg               <  1:36674/150‑2 (MQ=255)
                                                                                                                                       gtgGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGcc              <  2:1374602/150‑1 (MQ=255)
                                                                                                                                         ggTCCCGGTCATGATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAt            >  2:857897/1‑150 (MQ=255)
                                                                                                                                         ggTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAt            >  2:1699649/1‑150 (MQ=255)
                                                                                                                                         ggTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAt            >  2:354478/1‑150 (MQ=255)
                                                                                                                                          gTCCCGGTCATGATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAtt           >  2:1314802/1‑150 (MQ=255)
                                                                                                                                          gTCCCGGTCATCATGAACGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACAAGATATTCATCACGGAGTGACTATGTTACAGATAGTCTGCGCGCTGATCCAGATGATCTCACGATTTGCCAtt           >  1:781414/1‑150 (MQ=255)
                                                                                                                                           tCCCGGTCATGATGACCGGGACCACAGAGAGGCG‑‑ATGGTTCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAttct         >  1:119922/1‑150 (MQ=37)
                                                                                                                                             cccGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAttct         >  1:1527009/1‑150 (MQ=255)
                                                                                                                                              ccGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAttctt        >  1:1518720/1‑150 (MQ=255)
                                                                                                                                                   catcatGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCt   >  2:1394586/1‑150 (MQ=255)
                                                                                                                                                    atcatGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt  >  2:877248/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCTT  >  NC_000913/2074542‑2074838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: