Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,662 C→T 7.0% intergenic (‑137/+42) yeeE ← / ← plaP UPF0394 family inner membrane protein/putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,6620CT 7.0% 302.4 / 8.0 99intergenic (‑137/+42)yeeE/plaPUPF0394 family inner membrane protein/putrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base C (43/49);  new base T (5/2);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

Only 100 of 102 total aligned reads displayed.

CATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGC  >  NC_000913/2085514‑2085810
                                                                                                                                                    |                                                                                                                                                    
cATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGATcc                                                                                                                                                     >  2:17397/1‑150 (MQ=255)
cATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGcc                                                                                                                                                     >  2:733125/1‑150 (MQ=255)
cATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGcc                                                                                                                                                     >  2:130812/1‑150 (MQ=255)
    gAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAAt                                                                                                                                                 >  1:916776/1‑150 (MQ=255)
           tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                          >  2:1352240/1‑150 (MQ=255)
                taattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACt                                                                                                                                     >  2:361005/1‑150 (MQ=255)
                taataaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCAAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACt                                                                                                                                     <  2:885275/150‑1 (MQ=255)
                 aattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTc                                                                                                                                    <  2:1357530/150‑1 (MQ=255)
                 aattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTc                                                                                                                                    >  1:1648698/1‑150 (MQ=255)
                  attaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACAcc                                                                                                                                   >  2:1474871/1‑150 (MQ=255)
                  attaaACAGATTAATGTTAATTAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAATAAACCGGAGCCAAATGTTCATTCGACTcc                                                                                                                                   <  2:1488451/150‑1 (MQ=255)
                   ttaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCg                                                                                                                                  <  1:887053/150‑1 (MQ=255)
                       ccAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGttt                                                                                                                              <  2:777117/150‑1 (MQ=255)
                          gATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                           >  2:1333773/1‑150 (MQ=255)
                           aTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatta                                                                                                                          >  1:622303/1‑150 (MQ=255)
                           aTGAATGTTAATGAGGAAATAATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTAATTAATGAGGAGATCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatta                                                                                                                          <  1:1475116/150‑1 (MQ=255)
                            tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCTACTCCGGTttattat                                                                                                                         >  2:786508/1‑150 (MQ=255)
                            tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattat                                                                                                                         >  1:741963/1‑150 (MQ=255)
                               aTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAg                                                                                                                      <  1:151030/150‑1 (MQ=255)
                                         ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGAc                                                                                                                                      <  1:1664194/124‑1 (MQ=255)
                                         ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGAc                                                                                                                                      >  2:1664194/1‑124 (MQ=255)
                                                attCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTa                                                                                                     <  2:741963/150‑1 (MQ=255)
                                                attCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGAAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTa                                                                                                     <  2:553043/150‑1 (MQ=255)
                                                 ttCATGACTGGTTGAATTCAGACCAAAAACCAAATTCTATAAATTAGAACAAATGGTTATTAATGAGGATTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTAc                                                                                                    <  1:350956/150‑1 (MQ=255)
                                                        cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                             <  2:1513479/150‑1 (MQ=255)
                                                        cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                             <  2:402477/150‑1 (MQ=255)
                                                        cTGGTGGAATGCAGACCAAT‑ACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGt                                                                                            >  2:375931/1‑150 (MQ=255)
                                                        aagcttgAATGCATAACAATAACCAAATACTCAAAATAACAACAAATGGTTATTAATGAGGATTTCGATACAAAAGAATAAAAAAACGGGCGCCAAATGTTCATTCGACTCCTGATTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                             <  2:97469/144‑1 (MQ=255)
                                                           gtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGATATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTGCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTAc                                                                                          <  2:234712/150‑1 (MQ=255)
                                                            tggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACACCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACt                                                                                         <  2:1509755/150‑1 (MQ=255)
                                                             ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTg                                                                                        >  2:1454095/1‑150 (MQ=255)
                                                             ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGAATAAGCTACGTCTTCGTACTg                                                                                        >  1:1105501/1‑150 (MQ=255)
                                                              taaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGc                                                                                       <  1:379463/149‑1 (MQ=255)
                                                              gAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGc                                                                                       >  2:728615/1‑150 (MQ=255)
                                                               taTGCAGAACAATTATCAAATTCTCTTTATTAGTACATATGGTTATTATTGAGTTTTTCGATTCATAAGATTAAAAATTCCGTAGCGTTATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                      <  1:169164/149‑1 (MQ=255)
                                                               aaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                      <  2:623424/150‑1 (MQ=255)
                                                               aaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                      <  2:188733/150‑1 (MQ=255)
                                                                aTGCAGAGCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTAGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGCACTGCgg                                                                                     <  2:779609/150‑1 (MQ=255)
                                                                aTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTACGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCgg                                                                                     >  2:827314/1‑150 (MQ=255)
                                                                  gCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGaa                                                                                   >  2:23206/1‑150 (MQ=255)
                                                                    aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACAGGAGCCAGATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGATACGTCTTCGTACTGCGGAAcc                                                                                 >  1:1120551/1‑150 (MQ=255)
                                                                     gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACATCTTCGTACTGCGGAACCg                                                                                <  2:1711397/150‑1 (MQ=255)
                                                                     gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAAGGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTAGACTCCGGTTTATTATTAGAAAAGATGATTATGCTCCGTCTTCTTACTGCGGaccag                                                                                >  1:117004/1‑146 (MQ=255)
                                                                      aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCgg                                                                               <  2:1406269/150‑1 (MQ=255)
                                                                      aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCgg                                                                               <  2:408553/150‑1 (MQ=255)
                                                                       ccAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGa                                                                              >  2:616136/1‑150 (MQ=255)
                                                                          aTAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTa                                                                           >  1:1401453/1‑150 (MQ=255)
                                                                           tAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTAGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGAATCAGGTTTATTAATAGAAAAGATGATTATCCTAAGTCTTCTTACTGAGGAACCGGATTAc                                                                          >  1:1680669/1‑150 (MQ=255)
                                                                           aaaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTGCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTAc                                                                          <  2:1004256/149‑1 (MQ=255)
                                                                             aCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAATAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACgg                                                                        >  2:1347549/1‑150 (MQ=255)
                                                                              ccAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGa                                                                       <  2:459448/150‑1 (MQ=255)
                                                                                 aaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGc                                                                    <  1:530660/150‑1 (MQ=255)
                                                                                    tctcTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGc                                                                                                       >  1:688898/1‑112 (MQ=255)
                                                                                    tctcTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGc                                                                                                       <  2:688898/112‑1 (MQ=255)
                                                                                    tctcTAAATTAGAACAAATGCTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAAACGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGctct                                                                 >  1:255757/1‑150 (MQ=255)
                                                                                     ctctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCtt                                                                >  1:1047099/1‑150 (MQ=255)
                                                                                      tatAAAATAGAACAAAAGGTTATTAATGAGTAGTTCGATACAAAAGAAAAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTg                                                               <  2:1095880/148‑1 (MQ=255)
                                                                                        tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                             <  2:765138/150‑1 (MQ=255)
                                                                                        tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTTCTGCGGAACCGGATTACGGAAGCTCTTGGt                                                             <  2:1648698/150‑1 (MQ=255)
                                                                                        tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCAAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTATTGGt                                                             >  1:18234/1‑150 (MQ=255)
                                                                                            ttAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACg                                                         <  1:674581/150‑1 (MQ=255)
                                                                                              aGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATCATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCa                                                       >  2:913740/1‑150 (MQ=255)
                                                                                               gAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATAATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCaa                                                      >  2:1065168/1‑150 (MQ=255)
                                                                                                      tGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGt                                               <  2:1578779/150‑1 (MQ=255)
                                                                                                       ggTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTa                                              <  1:421338/150‑1 (MQ=255)
                                                                                                         ttattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTaaa                                            >  2:347257/1‑150 (MQ=255)
                                                                                                         ttattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTaaa                                            <  2:622303/150‑1 (MQ=255)
                                                                                                            ttaATGAGGAGTTCGATACAAAAGAATAATAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCa                                         <  1:1347549/150‑1 (MQ=255)
                                                                                                             taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAg                                        >  1:1131915/1‑150 (MQ=255)
                                                                                                             taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAg                                        <  1:361005/150‑1 (MQ=255)
                                                                                                              aaTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATTATTATGATACGTCTTCGTTCTGCGGATCCGGATTACGGAGGCTCTTGGGGACGCAAGCCCGGTAATTCAgc                                       >  2:325874/1‑149 (MQ=255)
                                                                                                                tGAGGTGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGTTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAACGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                     <  1:1604235/150‑1 (MQ=255)
                                                                                                                tGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                     <  2:916776/150‑1 (MQ=255)
                                                                                                                 gaggagTTCGATACAAAAGAATAAAAAAACCGGAGCCAATTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGATGGTCTTGGTGCCGAAAGCCAGGTAAATCAGCCCg                                    >  2:256216/1‑150 (MQ=255)
                                                                                                                     tgTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATag                                <  2:717579/149‑1 (MQ=255)
                                                                                                                      gTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATagc                               <  1:728615/150‑1 (MQ=255)
                                                                                                                        tCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGCCGCAAGCCAGGTAAATCAGACCGATagcag                             >  2:292398/1‑150 (MQ=255)
                                                                                                                         cGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTGGAAAAGAAGAGTATGCTACGTCTTCGTACTGCGGAACCGGAATACGGCAGCTCTTGGTGACGCCAGCCAGGTAAATCAGACCGATagcagc                            >  1:75370/1‑150 (MQ=255)
                                                                                                                            tACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCa                         <  1:733125/150‑1 (MQ=255)
                                                                                                                               aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                      >  2:1021901/1‑150 (MQ=255)
                                                                                                                               aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                      <  1:130812/150‑1 (MQ=255)
                                                                                                                                aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                     >  1:961186/1‑150 (MQ=255)
                                                                                                                                aaaGAATAAAAAAACCGGAGCCAAAAGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTATTGGTGGCGCAAGCCACGTAAATCAGAACTATAGCAGACAAGATc                     >  1:1326068/1‑150 (MQ=255)
                                                                                                                                   gAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGa                  >  1:585571/1‑150 (MQ=255)
                                                                                                                                   gAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGa                  >  1:658990/1‑150 (MQ=255)
                                                                                                                                    aaTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAc                 <  1:438280/150‑1 (MQ=255)
                                                                                                                                     aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAcc                >  1:1050225/1‑150 (MQ=255)
                                                                                                                                      tAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTAGGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc               <  1:493476/150‑1 (MQ=255)
                                                                                                                                         ataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa            >  1:1643936/3‑150 (MQ=39)
                                                                                                                                         aaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa            <  1:1454095/150‑1 (MQ=255)
                                                                                                                                            aaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc         >  1:380872/1‑150 (MQ=255)
                                                                                                                                            aaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTAGTACTGCGGAACCGGATTAAGGAAGCTCTTGGTGACGAAACCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCATAAcc         >  2:742102/1‑150 (MQ=255)
                                                                                                                                            aaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc         >  1:750132/1‑150 (MQ=255)
                                                                                                                                            aaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGAATATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc         <  1:225518/150‑1 (MQ=255)
                                                                                                                                             aCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCa        >  1:1455956/1‑150 (MQ=255)
                                                                                                                                              ccGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc               >  1:66309/1‑142 (MQ=255)
                                                                                                                                              ccGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc               <  2:66309/142‑1 (MQ=255)
                                                                                                                                                  aGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAg   <  2:353640/150‑1 (MQ=255)
                                                                                                                                                  aGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAg   <  2:348256/150‑1 (MQ=255)
                                                                                                                                                   gCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCGACGTCTTCGTACTGCGGAACCGGATTTCGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGc  >  1:632968/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGC  >  NC_000913/2085514‑2085810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑