Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,664 A→G 7.1% intergenic (‑139/+40) yeeE ← / ← plaP UPF0394 family inner membrane protein/putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,6640AG 7.1% 280.8 / 9.7 100intergenic (‑139/+40)yeeE/plaPUPF0394 family inner membrane protein/putrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base A (44/48);  new base G (5/2);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 101 total aligned reads displayed.

GAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTT  >  NC_000913/2085518‑2085813
                                                                                                                                                  |                                                                                                                                                     
gAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAAt                                                                                                                                                    >  1:916776/1‑150 (MQ=255)
       tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                             >  2:1352240/1‑150 (MQ=255)
            taattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACt                                                                                                                                        >  2:361005/1‑150 (MQ=255)
            taataaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCAAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACt                                                                                                                                        <  2:885275/150‑1 (MQ=255)
             aattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTc                                                                                                                                       >  1:1648698/1‑150 (MQ=255)
             aattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTc                                                                                                                                       <  2:1357530/150‑1 (MQ=255)
              attaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACAcc                                                                                                                                      >  2:1474871/1‑150 (MQ=255)
              attaaACAGATTAATGTTAATTAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAATAAACCGGAGCCAAATGTTCATTCGACTcc                                                                                                                                      <  2:1488451/150‑1 (MQ=255)
               ttaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCg                                                                                                                                     <  1:887053/150‑1 (MQ=255)
                   ccAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGttt                                                                                                                                 <  2:777117/150‑1 (MQ=255)
                      gATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                              >  2:1228579/1‑150 (MQ=255)
                      gATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                              >  2:1333773/1‑150 (MQ=255)
                       aTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatta                                                                                                                             >  1:622303/1‑150 (MQ=255)
                       aTGAATGTTAATGAGGAAATAATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTAATTAATGAGGAGATCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatta                                                                                                                             <  1:1475116/150‑1 (MQ=255)
                        tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCTACTCCGGTttattat                                                                                                                            >  2:786508/1‑150 (MQ=255)
                        tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattat                                                                                                                            >  1:741963/1‑150 (MQ=255)
                           aTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAg                                                                                                                         <  1:151030/150‑1 (MQ=255)
                                     ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGAc                                                                                                                                         <  1:1664194/124‑1 (MQ=255)
                                     ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGAc                                                                                                                                         >  2:1664194/1‑124 (MQ=255)
                                            attCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTa                                                                                                        <  2:741963/150‑1 (MQ=255)
                                            attCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGAAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTa                                                                                                        <  2:553043/150‑1 (MQ=255)
                                             ttCATGACTGGTTGAATTCAGACCAAAAACCAAATTCTATAAATTAGAACAAATGGTTATTAATGAGGATTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTAc                                                                                                       <  1:350956/150‑1 (MQ=255)
                                                    cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                <  2:1513479/150‑1 (MQ=255)
                                                    cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                <  2:402477/150‑1 (MQ=255)
                                                    cTGGTGGAATGCAGACCAATACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGt                                                                                               >  2:375931/1‑150 (MQ=255)
                                                    aagcttgAATGCATAACAATAACCAAATACTCAAAATAACAACAAATGGTTATTAATGAGGATTTCGATACAAAAGAATAAAAAAACGGGCGCCAAATGTTCATTCGACTCCTGATTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                <  2:97469/144‑1 (MQ=255)
                                                       gtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGATATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTGCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTAc                                                                                             <  2:234712/150‑1 (MQ=255)
                                                        tggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACACCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACt                                                                                            <  2:1509755/150‑1 (MQ=255)
                                                         ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTg                                                                                           >  2:1454095/1‑150 (MQ=255)
                                                         ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGAATAAGCTACGTCTTCGTACTg                                                                                           >  1:1105501/1‑150 (MQ=255)
                                                          taaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGc                                                                                          <  1:379463/149‑1 (MQ=255)
                                                          gAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGc                                                                                          >  2:728615/1‑150 (MQ=255)
                                                           taTGCAGAACAATTATCAAATTCTCTTTATTAGTACATATGGTTATTATTGAGTTTTTCGATTCATAAGATTAAAAATTCCGTAGCGTTATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                         <  1:169164/149‑1 (MQ=255)
                                                           aaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                         <  2:623424/150‑1 (MQ=255)
                                                           aaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                         <  2:188733/150‑1 (MQ=255)
                                                            aTGCAGAGCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTAGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGCACTGCgg                                                                                        <  2:779609/150‑1 (MQ=255)
                                                            aTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTACGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCgg                                                                                        >  2:827314/1‑150 (MQ=255)
                                                              gCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGaa                                                                                      >  2:23206/1‑150 (MQ=255)
                                                                aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACAGGAGCCAGATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGATACGTCTTCGTACTGCGGAAcc                                                                                    >  1:1120551/1‑150 (MQ=255)
                                                                 gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACATCTTCGTACTGCGGAACCg                                                                                   <  2:1711397/150‑1 (MQ=255)
                                                                 gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAAGGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTAGACTCCGGTTTATTATTAGAAAAGATGATTATGCTCCGTCTTCTTACTGCGGaccag                                                                                   >  1:117004/1‑146 (MQ=255)
                                                                  aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCgg                                                                                  <  2:408553/150‑1 (MQ=255)
                                                                  aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCgg                                                                                  <  2:1406269/150‑1 (MQ=255)
                                                                   ccAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGa                                                                                 >  2:616136/1‑150 (MQ=255)
                                                                      aTAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTa                                                                              >  1:1401453/1‑150 (MQ=255)
                                                                       tAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTAGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGAATCAGGTTTATTAATAGAAAAGATGATTATCCTAAGTCTTCTTACTGAGGAACCGGATTAc                                                                             >  1:1680669/1‑150 (MQ=255)
                                                                       aaaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTGCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTAc                                                                             <  2:1004256/149‑1 (MQ=255)
                                                                         aCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAATAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACgg                                                                           >  2:1347549/1‑150 (MQ=255)
                                                                          ccAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGa                                                                          <  2:459448/150‑1 (MQ=255)
                                                                             aaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGc                                                                       <  1:530660/150‑1 (MQ=255)
                                                                                tctcTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGc                                                                                                          <  2:688898/112‑1 (MQ=255)
                                                                                tctcTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGc                                                                                                          >  1:688898/1‑112 (MQ=255)
                                                                                tctcTAAATTAGAACAAATGCTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAAACGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGctct                                                                    >  1:255757/1‑150 (MQ=255)
                                                                                 ctctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCtt                                                                   >  1:1047099/1‑150 (MQ=255)
                                                                                  tatAAAATAGAACAAAAGGTTATTAATGAGTAGTTCGATACAAAAGAAAAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTg                                                                  <  2:1095880/148‑1 (MQ=255)
                                                                                    tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                                <  2:765138/150‑1 (MQ=255)
                                                                                    tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTTCTGCGGAACCGGATTACGGAAGCTCTTGGt                                                                <  2:1648698/150‑1 (MQ=255)
                                                                                    tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCAAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTATTGGt                                                                >  1:18234/1‑150 (MQ=255)
                                                                                        ttAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACg                                                            <  1:674581/150‑1 (MQ=255)
                                                                                          aGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATCATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCa                                                          >  2:913740/1‑150 (MQ=255)
                                                                                           gAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATAATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCaa                                                         >  2:1065168/1‑150 (MQ=255)
                                                                                                  tGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGt                                                  <  2:1578779/150‑1 (MQ=255)
                                                                                                   ggTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTa                                                 <  1:421338/150‑1 (MQ=255)
                                                                                                     ttattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTaaa                                               <  2:622303/150‑1 (MQ=255)
                                                                                                     ttattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTaaa                                               >  2:347257/1‑150 (MQ=255)
                                                                                                        ttaATGAGGAGTTCGATACAAAAGAATAATAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCa                                            <  1:1347549/150‑1 (MQ=255)
                                                                                                         taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAg                                           <  1:361005/150‑1 (MQ=255)
                                                                                                         taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAg                                           >  1:1131915/1‑150 (MQ=255)
                                                                                                          aaTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATTATTATGATACGTCTTCGTTCTGCGGATCCGGATTACGGAGGCTCTTGGGGACGCAAGCCCGGTAATTCAgc                                          >  2:325874/1‑149 (MQ=255)
                                                                                                            tGAGGTGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGTTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAACGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                        <  1:1604235/150‑1 (MQ=255)
                                                                                                            tGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                        <  2:916776/150‑1 (MQ=255)
                                                                                                             gaggagTTCGATACAAAAGAATAAAAAAACCGGAGCCAATTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGATGGTCTTGGTGCCGAAAGCCAGGTAAATCAGCCCg                                       >  2:256216/1‑150 (MQ=255)
                                                                                                                 tgTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATag                                   <  2:717579/149‑1 (MQ=255)
                                                                                                                  gTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATagc                                  <  1:728615/150‑1 (MQ=255)
                                                                                                                    tCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGCCGCAAGCCAGGTAAATCAGACCGATagcag                                >  2:292398/1‑150 (MQ=255)
                                                                                                                     cGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTGGAAAAGAAGAGTATGCTACGTCTTCGTACTGCGGAACCGGAATACGGCAGCTCTTGGTGACGCCAGCCAGGTAAATCAGACCGATagcagc                               >  1:75370/1‑150 (MQ=255)
                                                                                                                        tACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCa                            <  1:733125/150‑1 (MQ=255)
                                                                                                                           aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                         <  1:130812/150‑1 (MQ=255)
                                                                                                                           aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                         >  2:1021901/1‑150 (MQ=255)
                                                                                                                            aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                        >  1:961186/1‑150 (MQ=255)
                                                                                                                            aaaGAATAAAAAAACCGGAGCCAAAAGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTATTGGTGGCGCAAGCCACGTAAATCAGAACTATAGCAGACAAGATc                        >  1:1326068/1‑150 (MQ=255)
                                                                                                                               gAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGa                     >  1:585571/1‑150 (MQ=255)
                                                                                                                               gAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGa                     >  1:658990/1‑150 (MQ=255)
                                                                                                                                aaTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAc                    <  1:438280/150‑1 (MQ=255)
                                                                                                                                 aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAcc                   >  1:1050225/1‑150 (MQ=255)
                                                                                                                                  tAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTAGGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc                  <  1:493476/150‑1 (MQ=255)
                                                                                                                                     ataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa               >  1:1643936/3‑150 (MQ=39)
                                                                                                                                     aaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa               <  1:1454095/150‑1 (MQ=255)
                                                                                                                                        aaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc            >  1:380872/1‑150 (MQ=255)
                                                                                                                                        aaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTAGTACTGCGGAACCGGATTAAGGAAGCTCTTGGTGACGAAACCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCATAAcc            >  2:742102/1‑150 (MQ=255)
                                                                                                                                        aaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc            >  1:750132/1‑150 (MQ=255)
                                                                                                                                        aaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGAATATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc            <  1:225518/150‑1 (MQ=255)
                                                                                                                                         aCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCa           >  1:1455956/1‑150 (MQ=255)
                                                                                                                                          ccGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc                  <  2:66309/142‑1 (MQ=255)
                                                                                                                                          ccGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc                  >  1:66309/1‑142 (MQ=255)
                                                                                                                                              aGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAg      <  2:353640/150‑1 (MQ=255)
                                                                                                                                              aGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAg      <  2:348256/150‑1 (MQ=255)
                                                                                                                                               gCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCGACGTCTTCGTACTGCGGAACCGGATTTCGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGc     >  1:632968/1‑150 (MQ=255)
                                                                                                                                                 cAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCtt   >  1:784864/1‑150 (MQ=255)
                                                                                                                                                  aaaTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCttt  <  2:1680669/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTT  >  NC_000913/2085518‑2085813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: