Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,670 T→A 6.9% intergenic (‑145/+34) yeeE ← / ← plaP UPF0394 family inner membrane protein/putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,6700TA 6.9% 283.6 / 7.1 101intergenic (‑145/+34)yeeE/plaPUPF0394 family inner membrane protein/putrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base T (43/49);  new base A (5/2);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01

Only 100 of 101 total aligned reads displayed.

TAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTT  >  NC_000913/2085525‑2085816
                                                                                                                                                 |                                                                                                                                                  
tAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCAtt                                                                                                                                                >  2:1352240/1‑150 (MQ=255)
     taattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACt                                                                                                                                           >  2:361005/1‑150 (MQ=255)
     taataaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCAAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACt                                                                                                                                           <  2:885275/150‑1 (MQ=255)
      aattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTc                                                                                                                                          >  1:1648698/1‑150 (MQ=255)
      aattaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTc                                                                                                                                          <  2:1357530/150‑1 (MQ=255)
       attaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACAcc                                                                                                                                         >  2:1474871/1‑150 (MQ=255)
       attaaACAGATTAATGTTAATTAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAATAAACCGGAGCCAAATGTTCATTCGACTcc                                                                                                                                         <  2:1488451/150‑1 (MQ=255)
        ttaaCCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCg                                                                                                                                        <  1:887053/150‑1 (MQ=255)
            ccAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGTCGAATGAACATTTGGCTCCGGttt                                                                                                                                    <  2:777117/150‑1 (MQ=255)
               gATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                                 >  2:1228579/1‑150 (MQ=255)
               gATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatt                                                                                                                                 >  2:1333773/1‑150 (MQ=255)
                aTGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatta                                                                                                                                >  1:622303/1‑150 (MQ=255)
                aTGAATGTTAATGAGGAAATAATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTAATTAATGAGGAGATCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttatta                                                                                                                                <  1:1475116/150‑1 (MQ=255)
                 tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCTACTCCGGTttattat                                                                                                                               >  2:786508/1‑150 (MQ=255)
                 tGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTttattat                                                                                                                               >  1:741963/1‑150 (MQ=255)
                    aTGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAg                                                                                                                            <  1:151030/150‑1 (MQ=255)
                              ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGAc                                                                                                                                            >  2:1664194/1‑124 (MQ=255)
                              ggAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGAc                                                                                                                                            <  1:1664194/124‑1 (MQ=255)
                                     attCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTa                                                                                                           <  2:741963/150‑1 (MQ=255)
                                     attCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGAAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTa                                                                                                           <  2:553043/150‑1 (MQ=255)
                                      ttCATGACTGGTTGAATTCAGACCAAAAACCAAATTCTATAAATTAGAACAAATGGTTATTAATGAGGATTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTAc                                                                                                          <  1:350956/150‑1 (MQ=255)
                                             cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                   <  2:1513479/150‑1 (MQ=255)
                                             cTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                   <  2:402477/150‑1 (MQ=255)
                                             cTGGTGGAATGCAGACCAATACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGt                                                                                                  >  2:375931/1‑150 (MQ=255)
                                             aagcttgAATGCATAACAATAACCAAATACTCAAAATAACAACAAATGGTTATTAATGAGGATTTCGATACAAAAGAATAAAAAAACGGGCGCCAAATGTTCATTCGACTCCTGATTATTATTAGAAAAGATGATTATGCTACGTCTTCg                                                                                                   <  2:97469/144‑1 (MQ=255)
                                                gtggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGATATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTGCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTAc                                                                                                <  2:234712/150‑1 (MQ=255)
                                                 tggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACACCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACt                                                                                               <  2:1509755/150‑1 (MQ=255)
                                                  ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTg                                                                                              >  2:1454095/1‑150 (MQ=255)
                                                  ggAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGAATAAGCTACGTCTTCGTACTg                                                                                              >  1:1105501/1‑150 (MQ=255)
                                                   taaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGc                                                                                             <  1:379463/149‑1 (MQ=255)
                                                   gAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGc                                                                                             >  2:728615/1‑150 (MQ=255)
                                                    taTGCAGAACAATTATCAAATTCTCTTTATTAGTACATATGGTTATTATTGAGTTTTTCGATTCATAAGATTAAAAATTCCGTAGCGTTATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                            <  1:169164/149‑1 (MQ=255)
                                                    aaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                            <  2:623424/150‑1 (MQ=255)
                                                    aaTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCg                                                                                            <  2:188733/150‑1 (MQ=255)
                                                     aTGCAGAGCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTAGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGCACTGCgg                                                                                           <  2:779609/150‑1 (MQ=255)
                                                     aTGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTACGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCgg                                                                                           >  2:827314/1‑150 (MQ=255)
                                                       gCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGaa                                                                                         >  2:23206/1‑150 (MQ=255)
                                                         aGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACAGGAGCCAGATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGATACGTCTTCGTACTGCGGAAcc                                                                                       >  1:1120551/1‑150 (MQ=255)
                                                          gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACATCTTCGTACTGCGGAACCg                                                                                      <  2:1711397/150‑1 (MQ=255)
                                                          gACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAAGGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTAGACTCCGGTTTATTATTAGAAAAGATGATTATGCTCCGTCTTCTTACTGCGGaccag                                                                                      >  1:117004/1‑146 (MQ=255)
                                                           aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCgg                                                                                     <  2:408553/150‑1 (MQ=255)
                                                           aCCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCgg                                                                                     <  2:1406269/150‑1 (MQ=255)
                                                            ccAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGa                                                                                    >  2:616136/1‑150 (MQ=255)
                                                               aTAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTa                                                                                 >  1:1401453/1‑150 (MQ=255)
                                                                tAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTAGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGAATCAGGTTTATTAATAGAAAAGATGATTATCCTAAGTCTTCTTACTGAGGAACCGGATTAc                                                                                >  1:1680669/1‑150 (MQ=255)
                                                                aaaCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTGCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTAc                                                                                <  2:1004256/149‑1 (MQ=255)
                                                                  aCCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAATAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACgg                                                                              >  2:1347549/1‑150 (MQ=255)
                                                                   ccAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGa                                                                             <  2:459448/150‑1 (MQ=255)
                                                                      aaTTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGc                                                                          <  1:530660/150‑1 (MQ=255)
                                                                         tctcTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGc                                                                                                             >  1:688898/1‑112 (MQ=255)
                                                                         tctcTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGc                                                                                                             <  2:688898/112‑1 (MQ=255)
                                                                         tctcTAAATTAGAACAAATGCTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAAACGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGctct                                                                       >  1:255757/1‑150 (MQ=255)
                                                                          ctctAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCtt                                                                      >  1:1047099/1‑150 (MQ=255)
                                                                           tatAAAATAGAACAAAAGGTTATTAATGAGTAGTTCGATACAAAAGAAAAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTg                                                                     <  2:1095880/148‑1 (MQ=255)
                                                                             tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGt                                                                   <  2:765138/150‑1 (MQ=255)
                                                                             tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTTCTGCGGAACCGGATTACGGAAGCTCTTGGt                                                                   <  2:1648698/150‑1 (MQ=255)
                                                                             tAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCAAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTATTGGt                                                                   >  1:18234/1‑150 (MQ=255)
                                                                                 ttAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACg                                                               <  1:674581/150‑1 (MQ=255)
                                                                                   aGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATCATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCa                                                             >  2:913740/1‑150 (MQ=255)
                                                                                    gAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATAATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCaa                                                            >  2:1065168/1‑150 (MQ=255)
                                                                                           tGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGt                                                     <  2:1578779/150‑1 (MQ=255)
                                                                                            ggTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTa                                                    <  1:421338/150‑1 (MQ=255)
                                                                                              ttattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTaaa                                                  <  2:622303/150‑1 (MQ=255)
                                                                                              ttattaATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTaaa                                                  >  2:347257/1‑150 (MQ=255)
                                                                                                 ttaATGAGGAGTTCGATACAAAAGAATAATAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCa                                               <  1:1347549/150‑1 (MQ=255)
                                                                                                  taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAg                                              >  1:1131915/1‑150 (MQ=255)
                                                                                                  taATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAg                                              <  1:361005/150‑1 (MQ=255)
                                                                                                   aaTGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATTATTATGATACGTCTTCGTTCTGCGGATCCGGATTACGGAGGCTCTTGGGGACGCAAGCCCGGTAATTCAgc                                             >  2:325874/1‑149 (MQ=255)
                                                                                                     tGAGGTGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGTTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAAACGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                           <  1:1604235/150‑1 (MQ=255)
                                                                                                     tGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGAcc                                           <  2:916776/150‑1 (MQ=255)
                                                                                                      gaggagTTCGATACAAAAGAATAAAAAAACCGGAGCCAATTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGATGGTCTTGGTGCCGAAAGCCAGGTAAATCAGCCCg                                          >  2:256216/1‑150 (MQ=255)
                                                                                                          tgTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATag                                      <  2:717579/149‑1 (MQ=255)
                                                                                                           gTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATagc                                     <  1:728615/150‑1 (MQ=255)
                                                                                                             tCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGCCGCAAGCCAGGTAAATCAGACCGATagcag                                   >  2:292398/1‑150 (MQ=255)
                                                                                                              cGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTGGAAAAGAAGAGTATGCTACGTCTTCGTACTGCGGAACCGGAATACGGCAGCTCTTGGTGACGCCAGCCAGGTAAATCAGACCGATagcagc                                  >  1:75370/1‑150 (MQ=255)
                                                                                                                 tACAAAATAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCa                               <  1:733125/150‑1 (MQ=255)
                                                                                                                    aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                            <  1:130812/150‑1 (MQ=255)
                                                                                                                    aaaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGAt                            >  2:1021901/1‑150 (MQ=255)
                                                                                                                     aaaGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATc                           >  1:961186/1‑150 (MQ=255)
                                                                                                                     aaaGAATAAAAAAACCGGAGCCAAAAGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTATTGGTGGCGCAAGCCACGTAAATCAGAACTATAGCAGACAAGATc                           >  1:1326068/1‑150 (MQ=255)
                                                                                                                        gAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGa                        >  1:585571/1‑150 (MQ=255)
                                                                                                                        gAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGa                        >  1:658990/1‑150 (MQ=255)
                                                                                                                         aaTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAc                       <  1:438280/150‑1 (MQ=255)
                                                                                                                          aTAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAcc                      >  1:1050225/1‑150 (MQ=255)
                                                                                                                           tAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTAGGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc                     <  1:493476/150‑1 (MQ=255)
                                                                                                                              ataaaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa                  >  1:1643936/3‑150 (MQ=39)
                                                                                                                              aaaaaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGa                  <  1:1454095/150‑1 (MQ=255)
                                                                                                                                 aaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc               >  1:380872/1‑150 (MQ=255)
                                                                                                                                 aaCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTAGTACTGCGGAACCGGATTAAGGAAGCTCTTGGTGACGAAACCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCATAAcc               >  2:742102/1‑150 (MQ=255)
                                                                                                                                 aaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc               >  1:750132/1‑150 (MQ=255)
                                                                                                                                 aaCCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGAATATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAAcc               <  1:225518/150‑1 (MQ=255)
                                                                                                                                  aCCGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCa              >  1:1455956/1‑150 (MQ=255)
                                                                                                                                   ccGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc                     <  2:66309/142‑1 (MQ=255)
                                                                                                                                   ccGGAGTCGAATGAACATTTGGCTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGAccc                     >  1:66309/1‑142 (MQ=255)
                                                                                                                                       aGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAg         <  2:353640/150‑1 (MQ=255)
                                                                                                                                       aGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAg         <  2:348256/150‑1 (MQ=255)
                                                                                                                                        gCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCGACGTCTTCGTACTGCGGAACCGGATTTCGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGc        >  1:632968/1‑150 (MQ=255)
                                                                                                                                          cAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCtt      >  1:784864/1‑150 (MQ=255)
                                                                                                                                           aaaTGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCttt     <  2:1680669/150‑1 (MQ=255)
                                                                                                                                              tGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTttctt  <  2:585571/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TAAATTAATTAACCAGATGAATGTTAATGAGGAAATTATTCATGACTGGTGGAATGCAGACCAATAACCAAATTCTCTAAATTAGAACAAATGGTTATTAATGAGGAGTTCGATACAAAAGAATAAAAAAACCGGAGCCAAATGTTCATTCGACTCCGGTTTATTATTAGAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTT  >  NC_000913/2085525‑2085816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: