Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,099,256 G→T 10.8% P120T (CCC→ACC)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,099,2560GT10.8% 248.6 / 8.1 92P120T (CCC→ACC) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (41/40);  new base T (4/7);  total (45/47)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCAGCATCCCGGTAG  >  NC_000913/2099107‑2099404
                                                                                                                                                     |                                                                                                                                                    
aCGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACggg                                                                                                                                                      <  2:98574/150‑1 (MQ=255)
 cGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGa                                                                                                                                                     <  1:729198/150‑1 (MQ=255)
 cGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGa                                                                                                                                                     >  2:575701/1‑150 (MQ=255)
       tcgcgTTCTGAACGCTCACCGATGACAATACGTTAAGGATGGATATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCAGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGtt                                                                                                                                               <  1:845264/149‑1 (MQ=255)
        cgcgTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGttg                                                                                                                                              <  2:1253077/150‑1 (MQ=255)
        cgcgTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGttg                                                                                                                                              >  2:726445/1‑150 (MQ=255)
          cgTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGttgat                                                                                                                                            <  1:590411/150‑1 (MQ=255)
            ttCTGATCGCTCACCGATGACAATACGGGAAGCATGTAGATTATCGTAAACGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCTATATTTCTTATGCTTCGCTGCGGTAAAACCAACGGGTTCCGttgattt                                                                                                                                          <  2:73289/150‑1 (MQ=255)
            ttCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAATGGCGTTTACCAACCAGATGAAATACCGGGGA‑AATATAAAAATTTAATTGGGATATTTAATATGCATACCATCGGTAAAAACAACCGGAAACGtttaattt                                                                                                                                         >  1:853136/1‑144 (MQ=255)
             tCTGAACGCTCACCGCTGACAATACGGGAAGGATGGAGATTATCGTAACGGGCTTTCCCCTCACGGAGAAATTCCGGGGAGATTATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAATCAACGGTTCCCGttggtttt                                                                                                                                         <  2:1559191/150‑2 (MQ=255)
             tCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGTTCCCGttggtttt                                                                                                                                         <  1:1584156/150‑2 (MQ=255)
             tCTGAACGCTCAACGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGATTATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAATCAACGGTTCCCGttggtttt                                                                                                                                         <  2:1559192/150‑2 (MQ=255)
              cTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGttgatttga                                                                                                                                        <  1:842/150‑1 (MQ=255)
               tGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCAAGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCTCTGCGGTAAAACCAACGGGAACCGttgatttgat                                                                                                                                       <  2:656043/150‑1 (MQ=255)
                gAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAATCAACGGTTCCCGttggttttacc                                                                                                                                      <  2:489795/150‑5 (MQ=255)
                gAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGTTCCCGttggttttacc                                                                                                                                      <  1:339232/150‑5 (MQ=255)
                gAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTtgatg                                                                                                                                      <  1:1012724/150‑1 (MQ=255)
                gAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTtgatg                                                                                                                                      >  2:104646/1‑150 (MQ=255)
                gAACGCTAAACGATGACAATACGTGAAGGATGGAGATTATCGTAATAGGCTTTACCCTCACGTAGAAATTGCGTGGAGAATCAAATATTTTCAGTGCGATATTTCTTATGCATCGCTGAGGTTTTACCAACGGTAACCGTTGATTtgatg                                                                                                                                      <  2:256646/150‑1 (MQ=255)
                  aCGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGAc                                                                                                                                    <  1:1348432/150‑1 (MQ=255)
                        aCCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAc                                                                                                                              <  1:367002/150‑1 (MQ=255)
                         ccGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAcc                                                                                                                             >  1:456912/1‑150 (MQ=255)
                           gATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGc                                                                                                                           >  2:939231/1‑150 (MQ=255)
                              gACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATa                                                                                                                        <  1:385808/150‑1 (MQ=255)
                                cAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAg                                                                                                                      >  1:1566379/1‑150 (MQ=255)
                                cAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAg                                                                                                                      <  2:983904/150‑1 (MQ=255)
                                      gTGAAGGATGGATATTATCGTAAATGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTAAGCATCGATGCGGTAAAAACAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTa                                                                                                                <  1:281400/150‑1 (MQ=255)
                                       tGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACTATAACCGCATAAGGATTTAt                                                                                                               >  1:351028/1‑150 (MQ=255)
                                            gATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCaa                                                                                                          >  1:61034/1‑150 (MQ=255)
                                              tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACt                                                                                                        >  2:1164407/1‑150 (MQ=255)
                                                 agaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                                                     >  2:586638/1‑150 (MQ=255)
                                                 agaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                                                     <  2:1247420/150‑1 (MQ=255)
                                                 agaTTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                                                     <  2:616391/150‑1 (MQ=255)
                                                    ttATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCt                                                                                                  >  2:133226/1‑150 (MQ=255)
                                                     tATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCtt                                                                                                 >  2:1028851/1‑150 (MQ=255)
                                                      aTCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACggcgtt                                                                                                >  2:908932/1‑145 (MQ=255)
                                                         gTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAAt                                                                                             >  2:186513/1‑150 (MQ=255)
                                                         gTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAAt                                                                                             >  2:408214/1‑150 (MQ=255)
                                                                gCTTTACCCTCACGGAGAAATTGCGGGTATAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGAGGTTAAACCAACGTGAACCGTTGATTTGATTACCATAACCGCATAAGGATTTATGTCAACTACGTCTTTAATTACTGAt                                                                                      <  2:370800/150‑1 (MQ=255)
                                                                gCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATACCCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGAt                                                                                      >  2:104661/1‑150 (MQ=255)
                                                                gCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGAt                                                                                      >  2:1236589/1‑150 (MQ=255)
                                                                   ttACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCt                                                                                   >  2:513739/1‑150 (MQ=255)
                                                                        cTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACt                                                                              >  1:648573/1‑150 (MQ=255)
                                                                        cTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACt                                                                              <  1:1136478/150‑1 (MQ=255)
                                                                           aCGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGa                                                                           >  1:529875/1‑150 (MQ=255)
                                                                            cGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGAt                                                                          <  1:896815/150‑1 (MQ=255)
                                                                                  aaTTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTg                                                                    >  1:602303/1‑150 (MQ=255)
                                                                                    ttCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTTCTGATTCTACACTGGATGTATTGaa                                                                  <  2:853136/150‑1 (MQ=255)
                                                                                      ccGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaat                                                                >  1:1066680/1‑150 (MQ=255)
                                                                                           gagaATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAAACAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTa                                                           <  1:939231/150‑1 (MQ=255)
                                                                                               atataatatTTTCAGTGCGATTTTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGCCCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGttt                                                       >  1:1716848/1‑150 (MQ=255)
                                                                                                tataatatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGtttt                                                      <  2:543309/150‑1 (MQ=255)
                                                                                                tataatatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGtttt                                                      >  2:1647008/1‑150 (MQ=255)
                                                                                                 ataatatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTa                                                     <  2:17967/150‑1 (MQ=255)
                                                                                                  taatatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACTGGAACCGTTGATTTGATGACAATAACCGCATAAGGATTTATCTCAACTAAGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAg                                                    <  2:351028/150‑1 (MQ=255)
                                                                                                  taatatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATTACAATAAAATCATAAGGAATTAACTCAACTACTTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAg                                                    >  1:106185/1‑150 (MQ=255)
                                                                                                  taatatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAg                                                    <  1:1632370/150‑1 (MQ=255)
                                                                                                  taatatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAg                                                    <  1:104646/150‑1 (MQ=255)
                                                                                                   aatatTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAgg                                                   <  2:1566379/150‑1 (MQ=255)
                                                                                                        tttCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCAt                                              <  2:1410115/150‑1 (MQ=255)
                                                                                                        tttCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCAt                                              <  2:52289/150‑1 (MQ=255)
                                                                                                          tCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAg                                            <  1:575701/150‑1 (MQ=255)
                                                                                                             gTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCg                                         >  1:82704/1‑150 (MQ=255)
                                                                                                             gTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCg                                         >  2:1647906/1‑150 (MQ=255)
                                                                                                                cGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGtt                                      >  1:1090879/1‑150 (MQ=255)
                                                                                                                cGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGtt                                      >  2:1261876/1‑150 (MQ=255)
                                                                                                                    atTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAg                                  <  1:726445/150‑1 (MQ=255)
                                                                                                                      ttCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTg                                >  1:1308080/1‑150 (MQ=255)
                                                                                                                          tATGCAGCGCTGCGTTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCga                            <  2:707966/150‑1 (MQ=255)
                                                                                                                           aTGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCgat                           <  2:213553/150‑1 (MQ=255)
                                                                                                                            tGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCgatg                          >  1:1489597/1‑150 (MQ=255)
                                                                                                                            tGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCgatg                          >  2:1428891/1‑150 (MQ=255)
                                                                                                                              cATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCgatgat                        >  2:1630354/1‑150 (MQ=255)
                                                                                                                              cATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCgatgat                        <  2:456912/150‑1 (MQ=255)
                                                                                                                              cATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCgatgat                        >  2:1021671/1‑150 (MQ=255)
                                                                                                                              cATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCgatgat                        <  1:586638/150‑1 (MQ=255)
                                                                                                                               aTCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGatgatg                       >  2:614086/1‑150 (MQ=255)
                                                                                                                                  gCTGCGGTAAAACCAACTGGAACAGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACa                    <  2:507671/150‑1 (MQ=255)
                                                                                                                                  gCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGTCCATAACCGGATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACa                    <  2:1327296/150‑1 (MQ=255)
                                                                                                                                  gCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACa                    >  1:1264132/1‑150 (MQ=255)
                                                                                                                                  gCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACa                    <  1:186513/150‑1 (MQ=255)
                                                                                                                                  gCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACa                    >  1:697614/1‑150 (MQ=255)
                                                                                                                                    tGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATa                  >  2:169406/1‑150 (MQ=255)
                                                                                                                                     gtcatcaaaTCAACGGTTCCCGTTGGTTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATaa                 >  2:359252/7‑150 (MQ=255)
                                                                                                                                       ggTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATc               <  2:413692/150‑1 (MQ=255)
                                                                                                                                        gTAACACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGTTTTATCTCAACTACGTCTTTAATTACTGAATCTACACTGGATGTATTGAAATAATTAG‑TTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCAg             <  2:212596/150‑1 (MQ=255)
                                                                                                                                        gTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCa              <  2:82704/150‑1 (MQ=255)
                                                                                                                                         tcaaaTCAACGGTTCCCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTa                                                     <  2:771051/108‑1 (MQ=255)
                                                                                                                                         tcaaaTCAACGGTTCCCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTa                                                     >  1:771051/3‑110 (MQ=255)
                                                                                                                                          aaaaCCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCAGc            >  1:1142024/1‑150 (MQ=255)
                                                                                                                                           aaaTCAACGGTTCCCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCAGCa           >  2:516903/1‑150 (MQ=255)
                                                                                                                                           aaaTCAACGGTTCCCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCAGCa           >  1:477991/1‑150 (MQ=255)
                                                                                                                                             aCCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCAGCATc         >  2:1007068/1‑150 (MQ=255)
                                                                                                                                                   gggAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCAGCATCCCGGTa   >  1:900948/1‑150 (MQ=255)
                                                                                                                                                    ggAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCAGCATCCCGGTAg  >  1:1014917/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGAGTGGCGATGATGACATAATCAGCATCCCGGTAG  >  NC_000913/2099107‑2099404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑