Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,153,466 C→A 6.3% intergenic (+15/‑178) sibA → / → sibB sRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,4660CA 6.3% 354.3 / 6.9 118intergenic (+15/‑178)sibA/sibBsRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein
Reads supporting (aligned to +/- strand):  ref base C (63/47);  new base A (4/4);  total (67/51)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

AGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACA  >  NC_000913/2153318‑2153615
                                                                                                                                                    |                                                                                                                                                      
aGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTccc                                                                                                                                                       >  2:791699/1‑150 (MQ=255)
   gagaGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATATCCCTTTCCTTATTAAAAGCCATCTTCTCCGTGAGAGACATTACCGTTTCAGCGTCCCGCt                                                                                                                                                    >  2:825404/1‑150 (MQ=255)
   gagaGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCACTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCt                                                                                                                                                    >  1:507582/1‑150 (MQ=255)
   gagaGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCATTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCt                                                                                                                                                    >  2:1144186/1‑150 (MQ=255)
       ggTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCGGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                                                                                                >  1:307663/1‑150 (MQ=255)
       ggTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGaaa                                                                                                                                                >  2:1406305/1‑150 (MQ=255)
        ggttCCCGCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCAGCAGTATGATGCCGTGCGTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTGTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAat                                                                                                                                               <  2:371931/148‑1 (MQ=38)
        gTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaac                                                                                                                                               <  2:1034025/150‑2 (MQ=255)
         tttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaacg                                                                                                                                              <  1:116495/150‑3 (MQ=255)
          ttCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGGGACGCTGaaacgg                                                                                                                                             <  1:682636/150‑4 (MQ=255)
            ccccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAatcatc                                                                                                                                           <  1:791699/150‑1 (MQ=255)
             cccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCg                                                                                                                                          <  1:1556964/150‑1 (MQ=255)
              ccctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTACATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCgg                                                                                                                                         <  2:499738/150‑1 (MQ=255)
               cctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGc                                                                                                                                        <  1:894153/150‑1 (MQ=255)
               cctccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGc                                                                                                                                        <  1:833216/150‑1 (MQ=255)
                  ccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCCCCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTa                                                                                                                                     >  2:1570444/1‑150 (MQ=255)
                  ccccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTa                                                                                                                                     >  2:1576930/1‑150 (MQ=255)
                    ccctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTAcc                                                                                                                                   <  1:1346686/150‑1 (MQ=255)
                     cctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTAcca                                                                                                                                  >  1:1642191/1‑149 (MQ=255)
                      ctGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTAcctc                                                                                                                                 <  1:1485093/150‑1 (MQ=255)
                              cTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGcc                                                                                                                         >  2:424259/1‑150 (MQ=255)
                              cTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGATTACCTCATATCGcc                                                                                                                         >  2:194032/1‑150 (MQ=255)
                                  gTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCACCTTTCGCCCTGa                                                                                                                    >  1:639394/1‑150 (MQ=255)
                                    aaGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAAt                                                                                                                  <  2:1228847/150‑1 (MQ=255)
                                       ccTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCa                                                                                                               >  2:176975/1‑150 (MQ=255)
                                         tGGAAGATAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGt                                                                                                             <  1:1505019/150‑1 (MQ=255)
                                           gAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCt                                                                                                           <  2:638181/150‑1 (MQ=255)
                                               cTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAATTCATCGGCTTACCTCCTGTCGCCCTGAATGCAGTCTATCg                                                                                                       >  1:1488905/1‑150 (MQ=255)
                                                tAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGc                                                                                                      >  1:1257660/1‑150 (MQ=255)
                                                 aaTCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCt                                                                                                     <  2:818370/150‑1 (MQ=255)
                                                      cTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAAcgc                                                                                                >  2:913838/1‑150 (MQ=255)
                                                         agagTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTg                                                                                             <  2:271243/150‑1 (MQ=255)
                                                         agagTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTg                                                                                             >  1:382596/1‑150 (MQ=255)
                                                           agTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGaa                                                                                           <  2:509510/150‑1 (MQ=255)
                                                             tATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATa                                                                                         <  1:1103490/150‑1 (MQ=255)
                                                                cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTATCCCGTTTCAGCGTCACGCTGAAATCATCGGCTTACCTCCTTTCGACCTGAATGCAGTCTATCTCTAACTCGTTAATaaaa                                                                                      >  2:826082/1‑150 (MQ=37)
                                                                 aCCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAAt                                                                                     >  2:917683/1‑150 (MQ=255)
                                                                 aCCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAAt                                                                                     <  1:1708475/150‑1 (MQ=255)
                                                                 aCCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAAt                                                                                     >  1:1301627/1‑150 (MQ=255)
                                                                 aCCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATa                                                                                    >  2:1181116/1‑150 (MQ=255)
                                                                  ccAGTATGATGACGTGCTTCATCATACCCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATa                                                                                    >  1:82716/1‑150 (MQ=255)
                                                                      tATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCTGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGttt                                                                                <  1:743446/150‑1 (MQ=255)
                                                                        tgatgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTcc                                                                              <  2:1301627/150‑1 (MQ=255)
                                                                         gatgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCct                                                                             >  2:224498/1‑150 (MQ=255)
                                                                          atgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGTCTTACCTCCATTCGCCCTGAATTCAGTCTATCGCTTACGCGTGAATAAAATAGATTCctc                                                                            >  2:1134765/1‑150 (MQ=21)
                                                                          atgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCctc                                                                            <  1:1258602/150‑1 (MQ=255)
                                                                          atgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTGCCCGTTTAAGCGGCCCGCTGAAATCATGGGCTTACAGCCTTTGGCGCTGAATGCATTGTATAGCTTAGGCGTGAAGATATTAGtgtggta                                                                            >  2:1566306/1‑144 (MQ=21)
                                                                              cGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCtgtg                                                                        >  2:765804/1‑150 (MQ=255)
                                                                                  cTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATa                                                                                    <  2:851087/134‑1 (MQ=255)
                                                                                  cTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATa                                                                                    <  2:834396/134‑1 (MQ=255)
                                                                                  cTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATa                                                                                    >  1:851087/1‑134 (MQ=255)
                                                                                      atcatAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGc                                                                <  2:507582/150‑1 (MQ=255)
                                                                                           aaCCCTTTCCTTATTAAAATCCCTCTTCTCCTGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAAc                                                           <  1:813771/150‑1 (MQ=255)
                                                                                           aaCCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAAc                                                           <  1:224498/150‑1 (MQ=255)
                                                                                            aCCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACg                                                          <  1:1406305/150‑1 (MQ=255)
                                                                                             cccTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCGCTGAATGCAGTCTATCGATAACGCGTGAATAAATTAGTTTCCTCTGTGATTATTTCGTAACgc                                                         >  2:1563955/1‑149 (MQ=255)
                                                                                              ccTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGAc                                                        <  2:89210/150‑1 (MQ=255)
                                                                                                 ttCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCg                                                     <  2:307663/150‑1 (MQ=255)
                                                                                                 ttCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCg                                                     <  2:881896/150‑1 (MQ=255)
                                                                                                  tCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGc                                                    >  2:1611274/1‑150 (MQ=255)
                                                                                                  tCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGc                                                    >  1:53625/1‑150 (MQ=255)
                                                                                                      tattaAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAg                                                >  1:610134/1‑150 (MQ=255)
                                                                                                      tattaAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGAATCGCaaat                                                >  2:906449/1‑149 (MQ=255)
                                                                                                       attaAAAGCCCTCTACTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCATTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGATTAAACTAGTTTCCTCTGTGATTATTGCGTAACGACTCTCAAAGa                                               >  1:725783/1‑150 (MQ=25)
                                                                                                         taAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCAGAAATCAACGGCTTACCACCTTTCGCCATGAATGCAGTCAATCGCTAAGGCGTGAATAAAATAGTTTCATCTGTGATTATTGCGTAACGACTCGCATAGAat                                             >  1:1011304/1‑150 (MQ=25)
                                                                                                          aaaaGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATTGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAatt                                            >  2:717672/1‑149 (MQ=255)
                                                                                                          aaaaGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAata                                            >  1:729597/1‑150 (MQ=255)
                                                                                                            aaGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATAtt                                          <  2:534599/150‑1 (MQ=255)
                                                                                                             aGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATAttt                                         >  1:361594/1‑150 (MQ=255)
                                                                                                              gCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGt                                                              >  2:1069217/1‑128 (MQ=255)
                                                                                                              gCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGt                                                              <  1:1069217/128‑1 (MQ=255)
                                                                                                              gCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTg                                        <  1:962129/150‑1 (MQ=255)
                                                                                                              gCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTg                                        >  1:1331434/1‑150 (MQ=255)
                                                                                                               cccTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGc                                       <  2:1019152/150‑1 (MQ=255)
                                                                                                                     tctcCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAg                                 >  1:266902/1‑150 (MQ=255)
                                                                                                                      ctcCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAgg                                <  1:1108173/150‑1 (MQ=255)
                                                                                                                      ctcCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAgg                                >  1:718133/1‑150 (MQ=255)
                                                                                                                      ctcCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACACGCAAAGAATATTTGCTGGCAgg                                <  2:725681/150‑1 (MQ=255)
                                                                                                                        ccGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGAt                              >  1:1068710/1‑150 (MQ=255)
                                                                                                                          gggAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGCATATTTGATGGCAGGATCg                            >  1:279936/1‑150 (MQ=255)
                                                                                                                           ggAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGc                           <  2:1257660/150‑1 (MQ=255)
                                                                                                                            gagaGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGc                           >  2:1485942/1‑150 (MQ=255)
                                                                                                                             agagGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAg                         <  2:222294/150‑1 (MQ=255)
                                                                                                                              gagGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAgc                        >  2:126959/1‑149 (MQ=255)
                                                                                                                              gagGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGa                        >  1:900941/1‑150 (MQ=255)
                                                                                                                                ggCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACt                      <  1:1181116/150‑1 (MQ=255)
                                                                                                                                ggCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACt                      >  2:1265382/1‑150 (MQ=255)
                                                                                                                                  cTTTCCCGTTTCAGCGTCCCGCTGAAATCATAGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCGAACGCGTCAAAAAAATAGTTAACTCTGTGATGTTTGCGTAACGACTCGCAAAGAATATTTGCGGGCCGGATCGCAGACTAc                    >  2:1067005/1‑150 (MQ=25)
                                                                                                                                    ttCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACaa                  <  2:1668821/150‑1 (MQ=255)
                                                                                                                                     gatgatttCAGCGGGACGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACaaa                 >  1:477337/6‑150 (MQ=255)
                                                                                                                                        cGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGcc              >  1:1407279/1‑150 (MQ=255)
                                                                                                                                        cGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGcc              >  1:1242573/1‑150 (MQ=255)
                                                                                                                                         atttCAGCGGGACGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCt             >  1:1716791/2‑150 (MQ=255)
                                                                                                                                         atttCAGCGGGACGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCt             >  1:797125/2‑150 (MQ=255)
                                                                                                                                           ttCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGc           <  2:718133/150‑1 (MQ=255)
                                                                                                                                            tCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCg          >  1:336574/1‑150 (MQ=255)
                                                                                                                                            tCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCg          <  1:1553660/150‑1 (MQ=255)
                                                                                                                                             cAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCgg         >  2:551709/1‑150 (MQ=255)
                                                                                                                                                cGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGAtt      >  1:911734/1‑150 (MQ=255)
                                                                                                                                                    ccGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACa  >  1:905775/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
AGGGAGAGGTTTCCCCCTCCCCCTGGTGTTCTTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACA  >  NC_000913/2153318‑2153615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: