Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,168,423 A→T 6.9% intergenic (‑117/‑289) yegR ← / → yegS uncharacterized protein/phosphatidylglycerol kinase, metal‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,168,4230AT 6.9% 311.8 / 9.3 113intergenic (‑117/‑289)yegR/yegSuncharacterized protein/phosphatidylglycerol kinase, metal‑dependent
Reads supporting (aligned to +/- strand):  ref base A (49/56);  new base T (5/3);  total (54/59)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

Only 100 of 115 total aligned reads displayed.

ACTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCAT  >  NC_000913/2168274‑2168572
                                                                                                                                                     |                                                                                                                                                     
aCTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATaa                                                                                                                                                       <  1:773372/150‑1 (MQ=255)
 aaaattaattATATAGCAGCTATCTTTTTAATATCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAg                                                                                                                                                      <  2:1292755/148‑1 (MQ=255)
   aattaatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTa                                                                                                                                                    >  1:291666/1‑150 (MQ=255)
    attaatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAg                                                                                                                                                   >  2:211928/1‑150 (MQ=255)
      taatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGa                                                                                                                                                 <  2:299598/150‑1 (MQ=255)
      taatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACAATCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGa                                                                                                                                                 <  2:865271/150‑1 (MQ=255)
          gATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGaaatg                                                                                                                                             <  1:1475743/150‑4 (MQ=255)
           atatAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTAc                                                                                                                                            <  1:1070651/150‑1 (MQ=255)
            tataGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACg                                                                                                                                           <  2:1695684/150‑1 (MQ=255)
             ataGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACgg                                                                                                                                          >  1:69354/1‑150 (MQ=255)
             ataGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACgg                                                                                                                                          <  1:367116/150‑1 (MQ=255)
              taGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGaaatgttgt                                                                                                                                         <  2:1269650/150‑8 (MQ=255)
                   gcTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTCCAACATTTCATACATAAGTATTAATTACGGCCAttt                                                                                                                                    >  2:931716/1‑149 (MQ=255)
                   gcTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTg                                                                                                                                    >  2:756869/1‑150 (MQ=255)
                    cTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGa                                                                                                                                   <  2:1125049/150‑1 (MQ=255)
                     tATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAg                                                                                                                                  <  2:387405/150‑1 (MQ=255)
                      ttCTTTTTCATCTCTCCTTCCCCCATTAAATACCAACTACACTATCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACTACATTTCCTACATAAGTAGGAATTACGGACATTGAgg                                                                                                                                 <  1:1241601/149‑1 (MQ=255)
                        cTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACATTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGcc                                                                                                                               >  2:207043/1‑150 (MQ=255)
                        cTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGcc                                                                                                                               <  2:928959/150‑1 (MQ=255)
                          ttttCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCt                                                                                                                             >  1:81624/1‑150 (MQ=255)
                           tttCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATTTAATAATGGCATGATTATTATAATTGAATAGGATTATAATTAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCtt                                                                                                                            >  2:743557/1‑150 (MQ=255)
                               aTCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAgg                                                                                                                        <  2:688577/150‑1 (MQ=255)
                                 ctcACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTAAGGGt                                                                                                                      >  2:1140212/1‑150 (MQ=255)
                                  tcACCTTCCCCCAATAAATACCAACGACACTCTCTAGTGTTTAAATATAAAAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTa                                                                                                                     <  1:152214/150‑1 (MQ=255)
                                    aCCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGCAATTACGGACATTGAGGCCCTTCAGGGTAAc                                                                                                                   >  2:1089462/1‑150 (MQ=255)
                                     ccTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACt                                                                                                                  <  2:1278761/150‑1 (MQ=255)
                                           cccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATgg                                                                                                            >  1:1310567/1‑150 (MQ=255)
                                           cccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATgg                                                                                                            >  1:548819/1‑150 (MQ=255)
                                            ccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATggg                                                                                                           >  1:1149567/1‑150 (MQ=255)
                                            ccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATggg                                                                                                           >  2:699996/1‑150 (MQ=255)
                                             cATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATgggg                                                                                                          <  1:1552922/150‑1 (MQ=255)
                                               ttAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTATGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCTTgggggg                                                                                                        <  1:374146/150‑2 (MQ=255)
                                                 aaaTACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCtt                                                                                                      >  2:1104148/1‑150 (MQ=255)
                                                  aaTACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCttt                                                                                                     <  1:752257/150‑1 (MQ=255)
                                                  aaTACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATAGAGGCCCTTCAGGGTAACTCCATGGGGGCttt                                                                                                     >  2:149696/1‑150 (MQ=255)
                                                    tACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTaa                                                                                                   >  2:1001666/1‑150 (MQ=255)
                                                     aCCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAat                                                                                                  >  1:81700/1‑150 (MQ=255)
                                                     aCCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAat                                                                                                  <  2:1362824/150‑1 (MQ=255)
                                                      ccAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGTAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAata                                                                                                 <  1:130536/150‑1 (MQ=255)
                                                      ccAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAata                                                                                                 <  2:153868/150‑1 (MQ=255)
                                                      ccAACGACACTCTCTAGAGTATAAATATAATAATGGCATGATTATAATAATTGAATATGATTATAATAACTGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGTTAACTCCTTGGGGGCTTTAata                                                                                                 <  1:330505/150‑1 (MQ=255)
                                                       cAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGa                                                                                                                                                 <  1:1109748/101‑1 (MQ=255)
                                                       cAACGACACTCTCTAGTGTTAAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAatat                                                                                                <  1:105498/150‑1 (MQ=255)
                                                        aaCGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAatata                                                                                               >  2:1683256/1‑150 (MQ=255)
                                                              acTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAAGTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGTCCCTTCAGGGTAACTCCAGGGGCGCTTTAAtatattatat                                                                                         >  2:277784/1‑150 (MQ=255)
                                                                 ctctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGa                                                                                      >  2:1031158/1‑150 (MQ=255)
                                                                  tctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGaa                                                                                     >  2:743572/1‑150 (MQ=255)
                                                                   ctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAATTAGGAATTAAGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAg                                                                                    <  1:157590/150‑1 (MQ=255)
                                                                   ctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAg                                                                                    >  2:1177628/1‑150 (MQ=255)
                                                                    tAGTGTTTAAATATAATAATGGCATTATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGa                                                                                   <  2:1513384/150‑1 (MQ=255)
                                                                    tAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGa                                                                                   <  2:291666/150‑1 (MQ=255)
                                                                      gtgtTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATg                                                                                 >  1:27646/1‑150 (MQ=255)
                                                                      gtgtTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATg                                                                                 <  1:1477235/150‑1 (MQ=255)
                                                                      gtgtTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATg                                                                                 <  1:1104148/150‑1 (MQ=255)
                                                                      gtgtTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTCAATATAATATATTAAAgccc                                                                                 <  1:72352/150‑4 (MQ=255)
                                                                        gtTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGcc                                                                               >  1:321931/1‑150 (MQ=255)
                                                                         tttAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCCTTAATATATTATATTGAAGATGCCa                                                                              >  2:976221/1‑150 (MQ=255)
                                                                               tataATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGttt                                                                        <  1:1553958/150‑1 (MQ=255)
                                                                               tataATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGttt                                                                        <  1:614033/150‑1 (MQ=255)
                                                                               tataATAATGGCATGATTATTATAATTGAATAGGATTATAAAAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGACTTTAATATATTATATTGAAGATGCCACTGttt                                                                        <  2:1302311/150‑1 (MQ=255)
                                                                                  aataatGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGt                                                                     <  1:1031158/150‑1 (MQ=255)
                                                                                  aataatGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGt                                                                     <  2:1699923/150‑1 (MQ=255)
                                                                                  aataatGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAAATATTATATTGAACATGCCACTCTTTAGt                                                                     >  2:40469/1‑150 (MQ=255)
                                                                                    taatGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg                                                                   <  1:1140212/150‑1 (MQ=255)
                                                                                    taatGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg                                                                   <  1:207043/150‑1 (MQ=255)
                                                                                      atGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGaa                                                                 >  1:1714421/1‑150 (MQ=255)
                                                                                       tGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGTGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAat                                                                <  1:1717927/150‑1 (MQ=255)
                                                                                           aTGATTATTATAATTTAATAGGATTATAATAAAAGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCGTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa                                                            <  2:225329/150‑1 (MQ=255)
                                                                                           aTGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa                                                            >  1:1085501/1‑150 (MQ=255)
                                                                                              attattATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGt                                                         <  2:1089281/150‑1 (MQ=255)
                                                                                              attattATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGt                                                         <  2:155029/150‑1 (MQ=255)
                                                                                                    ataATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGGCCTTCAGTGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGc                                                   >  2:1201558/1‑150 (MQ=255)
                                                                                                    ataATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGc                                                   <  2:65387/150‑1 (MQ=255)
                                                                                                      aaTTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGctc                                                 <  1:1683256/150‑1 (MQ=255)
                                                                                                       aTTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGctct                                                >  2:1451525/1‑150 (MQ=255)
                                                                                                           aaTAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATTTTAGGTATATGCTCttttt                                            >  2:1633488/1‑150 (MQ=255)
                                                                                                             tatgATTATATTAAATGTTGTTTACAACATTTCCTACATAAGTATGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCTTGGGGGCTTTAATATATTATTTTGTAGATGCCACTGTTTAGTTGATTATTAGGTATATGCTCTTTTTTg                                          <  1:1555856/147‑1 (MQ=255)
                                                                                                             tAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTg                                          <  1:974966/150‑1 (MQ=255)
                                                                                                                       ataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCg                                >  2:1361469/1‑150 (MQ=255)
                                                                                                                        tggaTGATCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCgg                               <  2:314353/147‑1 (MQ=255)
                                                                                                                           aTGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTgg                            >  2:1567836/1‑150 (MQ=255)
                                                                                                                            tGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGATTATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGc                           >  2:1586553/1‑150 (MQ=255)
                                                                                                                               tCTGTACAACATTTCCTACATAAGTAGGAATTACTGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGc                        <  2:548819/150‑1 (MQ=255)
                                                                                                                                 tGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCg                      <  2:799816/150‑1 (MQ=255)
                                                                                                                                    acaacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTAAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTa                   >  2:474199/1‑150 (MQ=255)
                                                                                                                                     caacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAg                  >  2:593773/1‑150 (MQ=255)
                                                                                                                                     caacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAg                  >  2:1684232/1‑150 (MQ=255)
                                                                                                                                      cgtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATAAGCTCTTTTTTGAAATTTATCGTTGGCAGCCGTTAGt                 >  2:35654/6‑150 (MQ=255)
                                                                                                                                       gtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGATTATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTa                >  1:1265553/5‑150 (MQ=255)
                                                                                                                                       gtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTa                >  2:952840/5‑150 (MQ=255)
                                                                                                                                       gtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTa                >  2:105505/5‑150 (MQ=255)
                                                                                                                                          tttCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTc             >  2:1128494/1‑150 (MQ=255)
                                                                                                                                          tttCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTc             >  1:1235385/1‑150 (MQ=255)
                                                                                                                                          tttCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTc             <  2:81700/150‑1 (MQ=255)
                                                                                                                                           ttCCTACATAAGTAGGAATTAAGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTCTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAATTTTATCGGTGGCAGCCGTTAGTATTCg            >  2:1309399/1‑150 (MQ=255)
                                                                                                                                               tACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTACATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGt        <  1:1177628/150‑1 (MQ=255)
                                                                                                                                                aCATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTc       >  2:1302199/1‑150 (MQ=255)
                                                                                                                                                aCATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTc       >  1:645767/1‑150 (MQ=255)
                                                                                                                                                aCATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTc       <  2:1149567/150‑1 (MQ=255)
                                                                                                                                                     aGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCACTGTCCCCAt  <  2:1144591/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCCCAT  >  NC_000913/2168274‑2168572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: